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  • Myf5
  • A subpopulation of Pax7 + satellite cells that does not express Myf5 has been proposed to be responsible for repopulating the stem cell niche in the regenerated muscle and once again become quiescent ( 26 ). (physiology.org)
  • 2008). Yet, ~10% of Pax7-expressing SCs, which have never expressed Myf5, show a privileged contribution to the SC compartment compared to Pax7-positive, Myf5-expressing cells indicating heterogeneity within the SC population (Kuang et al. (a-443654.com)
  • 2007). Critically missing from previous studies is the role of Mouse monoclonal to TLR2 Pax7 in long-term maintenance and expansion of these heterogeneous populations of muscle stem cells defined by Myf5 expression. (a-443654.com)
  • In addition to anatomic location, satellite cells are typified by markers such as M-cadherin, Pax7, Myf5, and neural cell adhesion molecule-1. (pitt.edu)
  • Regulation
  • Skeletal muscle is composed of long multinucleated cells, muscle fibers (myofibers), which function as primary effectors for force production and contribute to the regulation of whole body metabolism. (elifesciences.org)
  • demonstrate the importance of peripheral clock regulation in chondrocytes and report that the circadian transcription factor BMAL1 plays an essential role in maintaining the integrity of cartilage tissue. (jci.org)
  • These results indicate the effects of resistance exercise on the regulation of satellite cells and provide insight into the molecular mechanism of satellite cell activation following physical exercise. (hindawi.com)
  • Exercise positively affects muscle fiber composition via regulation of satellite cells to improve muscle performance. (hindawi.com)
  • Fei J, Tamski H, Cook C, Santanam N (2013) MicroRNA regulation of adipose derived stem cells in aging rats. (springer.com)
  • Because of their potential to regulate a large number of protein-encoding genes, miRNAs have been implicated in numerous biological processes, including development, stem cell regulation and human diseases. (biologists.org)
  • The investigation of the hitherto unappreciated layer of gene regulation mediated by miRNAs has also provided new insights into our understanding of stem cell biology and pointed to new directions for therapeutic applications of stem cells. (biologists.org)
  • Moreover, previous studies have shown that altered expression levels of miRNAs can contribute to pathological conditions, such as cancer, due to the loss of cell cycle regulation. (springer.com)
  • However, the precise mechanism underlying miRNA-mediated regulation of cell cycle in stem cells is still incompletely understood. (springer.com)
  • Later on Mitf and Chx10 continue to be expressed in adjacent regions of the eye, suggesting a reciprocal regulation between these two factors ( Nguyen and Arnheiter, 2000 ). (biologists.org)
  • To fully harness the therapeutic value of these cells for cartilage repair, an in-depth understanding of the molecular regulation of chondrogenesis is essential. (biomedcentral.com)
  • multipotent
  • It has been shown that the bulge region of the upper hair follicle is a repository of multipotent stem cells. (medsci.org)
  • Throughout their lifetime, tissue-specific, multipotent somatic stem cells maintain homeostasis of individual tissues and organs. (biologists.org)
  • markers
  • Cells coexpressing myogenic and endothelial cell markers (CD56, CD34, CD144) were identified by immunohistochemistry and flow cytometry. (pitt.edu)
  • This protocol employs fractionation strategies to deplete cells expressing the cell surface markers CD45, CD31, CD11b and Ly-6A/E-Sca1, both by magnetic separation and FACS-based exclusion, and positively select for cells expressing a7-integrin and CD34. (bio-protocol.org)
  • Moreover
  • Moreover, we find that a family of salamander methyltransferases is expressed specifically in adult appendages. (diva-portal.org)
  • Moreover, bulge stem cells can give rise to different epithelial cell types of hair follicles and participate in the formation of hair follicles, epidermis and sebaceous glands. (medsci.org)
  • protein
  • These mice had modifications to their muscle stem cells that allowed the number of these cells to be artificially reduced, and some cells also produced a fluorescent protein that allowed them to be tracked. (elifesciences.org)
  • TNF receptor-associated factor 6 (TRAF6) is an adaptor protein and an E3 ubiquitin ligase that mediates the activation of multiple cell signaling pathways in a context-dependent manner. (jci.org)
  • Lymphoid enhancer binding factor-1 (Lef1) is an essential regulatory protein in the Wnt signal pathway, which controls cell growth and differentiation. (medsci.org)
  • Recombinant plasmids were transformed into BL21 competent cells (Stratagene, La Jolla, CA, USA) and purified protein was obtained by glutathione column affinity chromatography . (world-of-links.com)
  • When a cell enters the G1 phase, a protein called cyclin D increases in response to mitogenic stimuli. (springer.com)
  • To determine the effect of high YAP activity on chondrogenesis, C3H10T1/2 MSC-like cells were transduced with human (h)YAP and treated in micromass with bone morphogenetic protein-2 (BMP-2). (biomedcentral.com)
  • bone marrow
  • These include resident endothelial cells that restore capillary bed function during sprouting angiogenesis ( 5 ), as well as other stem cell populations from the bone marrow compartment ( 57 ). (physiology.org)
  • Molecular
  • Furthermore, the molecular mechanisms by which exercise-stimulating extracellular factors control the satellite cell activation and differentiation remain unclear. (hindawi.com)
  • Karolinska Institute, Department of Cell and Molecular Biology. (diva-portal.org)
  • We thus identified potential molecular mediators of the inhibitory effects of SOD1 G93A on myogenic program and disclosed potential signaling, activated by SOD1 G93A , that affect the identity of the myogenic cell population. (hindawi.com)
  • This manuscript documents anatomic, molecular and developmental relationships between endothelial and myogenic cells within human skeletal muscle. (pitt.edu)
  • Elucidation of the molecular mechanisms underlying myogenic differentiation has been greatly facilitated by the availability of several immortalized myogenic cell lines, including C2C12 cells, which are derived from mouse MSCs ( Yaffe and Saxel, 1977 ). (rupress.org)
  • Clarifying the developmental lineage of specific cell populations is fundamental to understanding syndromic diseases and to unravel the molecular etiology and relationship between various phenotypic outcomes. (jneurosci.org)
  • postnatal
  • Deletion of in mice leads to normal numbers of stem cells 1211441-98-3 at birth followed by excessive wasting of stem cells during the first weeks of postnatal development (Oustanina et al. (a-443654.com)
  • mRNA
  • Concomitant with the loss of Pax7-positive SCs, we also noted a rapid decline of Pax7 mRNA concentrations in TAM-treated Pax7CE/loxP-Gu mice (Figures S3A and S3F) and a rapid decline of SCs as assessed by the expression of calcitonin receptor (Calcr), a marker. (a-443654.com)
  • In the present study, we compared mRNA expression of these factors between the two types. (semanticscholar.org)
  • demonstrate
  • In the present study, we utilize transgenic technology and demonstrate that the 4.6 kb upstream fragment of the Foxk1 gene directs β‐galactosidase expression to the myogenic progenitor cell population. (embopress.org)
  • These data demonstrate that Schwann cells and olfactory ensheathing cells share a common developmental origin. (jneurosci.org)
  • By using two complementary mouse models, we demonstrate the NC origin of the OECs and an NC contribution to subsets of GnRH-1 neurons and cells of the olfactory epithelium (OE) and vomeronasal organ (VNO). (jneurosci.org)
  • zebrafish
  • The pattern of alternating blue and golden stripes displayed by adult zebrafish is composed of three kinds of pigment cells: black melanophores, yellow xanthophores, and silvery-blue iridophores. (zfin.org)
  • miRNAs
  • In the present chapter, we present a brief overview of these studies and discuss their contributions toward our understanding of the importance of miRNAs in normal and aged stem cell function in various model systems. (springer.com)
  • In this Commentary, we review the most recent developments in our understanding of the biological role of miRNAs in the differentiation and maintenance of mammalian stem cells, discuss lessons learned from recent studies and offer our views on future challenges. (biologists.org)
  • Several miRNAs have been suggested to target transcripts that directly or indirectly coordinate the cell cycle progression of stem cells. (springer.com)
  • In this review, we discuss current knowledge of miRNAs regulatory role in cell cycle progression of stem cells. (springer.com)
  • We describe how specific miRNAs may control cell cycle associated molecules and checkpoints in embryonic, somatic and cancer stem cells. (springer.com)
  • We further outline how these miRNAs could be regulated to influence cell cycle progression in stem cells as a potential clinical application. (springer.com)
  • pluripotent
  • At early developmental stages, pluripotent NC cells migrate throughout the organism and give rise to many derivates including neurons and glia of the peripheral nervous system, bone and cartilage of the skull, and melanocytes ( Bronner-Fraser, 1995 ). (jneurosci.org)