Adult stem cells myoblasts progenitor satellite cell. Medical search. Frequent questions

Myoblasts cultured using MyoCult™-SF Expansion Medium can be differentiated into multinucleated myotubes using MyoCult™ Differentiation Kit (Human) . (stemcell.com)
The embryonic AMPs are at the origin of all adult fly muscles and, as we demonstrate here, they express repressors of myogenic differentiation and targets of the Notch pathway known to be involved in muscle cell stemness. (biologists.com)
PKD is a family of serine-threonine kinases that regulate gene expression, protein secretion, cell proliferation, differentiation and inflammation. (bvsalud.org)
Functional assays showed that circIGF1R promoted myoblast differentiation of porcine skeletal muscle satellite cells (SMSCs), while it had no effect on cell proliferation. (bvsalud.org)
Furthermore, the rescue experiments showed that circIGF1R could counteract the inhibitory effect of miR-16 on cell myoblast differentiation. (bvsalud.org)
In conclusion, this study successfully screened candidate circRNAs involved in the regulation of porcine myogenesis and demonstrated that circIGF1R promotes myoblast differentiation via miR-16, which lays a theoretical foundation for understanding the role and mechanism of circRNAs in regulating porcine myoblast differentiation. (bvsalud.org)
The proliferation and differentiation of satellite cells are regulated by a number of extracellular signals ( Wang & Rudnicki, 2012 Wang YX, Rudnicki MA. (scielo.br)
A number of different local transcription factors called myogenic regulatory factors (MRFs), including MyoD, myf-5, myogenin, and myf-6, stimulate proliferation and differentiation of satellite cells [ 9 ]. (hindawi.com)
In addition, insulin, such as growth factor (IGF) 1Ea and/or IGF-1Ec (MGF), also stimulates proliferation and differentiation of satellite cells. (hindawi.com)
During stem cell cardiac differentiation, a distinct swish in the expression profile of laminin subunits has been reported with laminin-411/421 pre-dominantly expressed early in progenitors and laminin-211/221 expressed later in cardiomyocytes (Ja, 2015). (biolamina.com)
A highly reproducible, chemically defined, xeno-free laminin-based differentiation protocol to generate stem cell-derived cardiovascular progenitors (CVPs) was reported in 2019 (Yap, 2019). (biolamina.com)
Laminin-221 (LN221) was identified as the most likely expressed cardiac laminin and the authors show that this protein promotes differentiation of pluripotent human embryonic stem cells (hESCs) toward cardiomyocyte lineage and downregulates pluripotency and teratoma-associated genes. (biolamina.com)
This in accordance with recent data presented by researchers at Icagen Inc., demonstrating that the laminin-521 cell culture matrix maintains the differentiation potential of mouse and human satellite cell-derived myoblasts, even during long-term culture expansion (Penton, 2016). (biolamina.com)
Laminin-521 supports increased differentiation potential without altering the traditional Pax7/MyoD paradigm and the results are translational across several mouse backgrounds, human cells, and disease states. (biolamina.com)
All cells have been tested for myotube differentiation. (zen-bio.com)
Human skeletal myoblast are differentiated using Skeletal Muscle Differentiation Medium (cat# SKM-D) for at least 6 days. (zen-bio.com)
Specially formulated media are available for maintenance and differentiation of our adult skeletal myoblasts. (zen-bio.com)
The transcription factor CCAAT/enhancer binding protein beta (C/EBPβ) is expressed in Pax7 + satellite cells of healthy muscle and is downregulated during myoblast differentiation. (biomedcentral.com)
We find that loss of C/EBPβ leads to precocious differentiation at the expense of self-renewal in primary myoblast and myofiber cultures. (biomedcentral.com)
We find that C/EBPβ can regulate Notch2 expression and that restoration of Notch activity in myoblasts lacking C/EBPβ prevents precocious differentiation. (biomedcentral.com)
When overexpressed in the murine myoblast cell line C2C12 or in primary myoblasts, C/EBPβ reduced MyoD and other myogenic protein levels during differentiation, in addition to reducing myoblast fusion. (biomedcentral.com)
Loss of C/EBPβ in SCs results in precocious differentiation at the expense of self-renewal in both primary myoblasts and myofiber cultures. (biomedcentral.com)
IL-2/IL2R also promotes the differentiation of T cells into effector T cells. (ilreceptor.com)
Conversely, ectopic expression of PRDM16 in myoblasts induces their differentiation into brown fat cells. (bioseek.eu)
Fracture Healing in Diabetes Fracture repair entails recruitment of mesenchymal stem cells after which differentiation to chondrocytes that type cartilage (see Chapter 12). (dnahelix.com)
Deletion of HP1β, but not HP1α, in ESCs provokes a loss of the morphological and proliferative characteristics of embryonic pluripotent cells, reduces expression of pluripotency factors and causes aberrant differentiation. (biomedcentral.com)
However, in differentiated cells, loss of HP1β has the opposite effect, perturbing maintenance of the differentiation state and facilitating reprogramming to an induced pluripotent state. (biomedcentral.com)
We demonstrate an unexpected duality in the role of HP1β: it is essential in ESCs for maintaining pluripotency, while it is required for proper differentiation in differentiated cells. (biomedcentral.com)
This unique duality makes them an attractive system for potential regenerative medicine and cell therapies, but also for differentiation studies in vitro and for modeling diseases. (biomedcentral.com)
Serra M, Brito C and Alves PM. "Bioengineering strategies for stem cell expansion and differentiation. (sciepub.com)
Khadra M, Lyngstadaas SP, Haanaes HR, Mustafa K. "Effect of laser therapy on attachment, proliferation and differentiation of human osteoblast-like cells cultured on titanium implant material. (sciepub.com)
Therefore, promotion of myoblast proliferation and differentiation and induction of myotube hypertrophy should be beneficial for muscle regeneration and control of muscle mass. (kosfaj.org)

ENGLISH ABSTRACT: Introduction: Adult mammalian tissue regeneration recruits progenitor stem cells. (sun.ac.za)
Fibroblast Growth Factors (FGFs) are fundamental in embryonic development but also in adult skeletal muscle regeneration from injury or pathology. (sun.ac.za)
Satellite cells are essential for skeletal muscle regeneration:the cell on the edge returns centre stage. (scielo.br)
Regeneration of muscle after injury normally occurs rapidly with satellite stem cells that reside in the space between the basal lamina and the sarcolemma, playing a major role [ 11 ]. (hindawi.com)
In 2009 beginning of 2010 we have focused on investigating what factors human embryonic stem cells (hESCs) may produce that enhance regeneration and if those factors have any effects by themselves on regeneration. (ca.gov)
Finally, our data suggest that muscle stem cells either do not accumulate DNA damage with age or can efficiently repair such damage, when activated for tissue regeneration. (ca.gov)
Direct isolation of satellite cells for skeletal muscle regeneration. (medecinesciences.org)
Alameddine HS, Dehaupas M, Fardeau M. Regeneration of skeletal muscle fibers from autologous satellite cells multiplied in vitro. (medecinesciences.org)
Identification of a novel population of muscle stem cells in mice: potential for muscle regeneration. (medecinesciences.org)
Using genetic tools to conditionally abrogate C/EBPβ expression in Pax7 + cells, we examined the role of C/EBPβ in self-renewal of satellite cells during muscle regeneration. (biomedcentral.com)
After a single muscle injury, C/EBPβ-deficient satellite cells fail to self-renew resulting in a reduction of satellite cells available for future rounds of regeneration. (biomedcentral.com)
These findings demonstrate that C/EBPβ is a novel regulator of satellite cell self-renewal during muscle regeneration acting at least in part through Notch2. (biomedcentral.com)
In this study, we investigate the effects of neural cell integration into the bioprinted skeletal muscle construct to accelerate functional muscle regeneration in vivo. (nature.com)
Myofiber-specific ablation of IRE1α or XBP1 in mice diminishes skeletal muscle regeneration that is accompanied with reduced number of satellite cells. (elifesciences.org)
Finally, targeted ablation of IRE1α also reduces Notch signaling, abundance of satellite cells, and skeletal muscle regeneration in the mdx mice, a model of Duchenne muscular dystrophy. (elifesciences.org)
Collectively, our experiments suggest that the IRE1α-mediated signaling promotes muscle regeneration through augmenting the proliferation of satellite cells in a cell non-autonomous manner. (elifesciences.org)
The findings shed new light on mechanisms of regeneration of healthy myofibers after severe tissue injury and suggest interplay between these fibers and muscle satellite cells though mechanisms remain to be elucidated. (elifesciences.org)
Injection injury activates regeneration of host myofibers that fuse with the engrafted myoblasts, sharing their nuclei in a common gene pool of the syncytium. (jgc301.com)
One of the great advantages of tissue engineering of skeletal muscle is that adult skeletal muscle contains many myogenic precursor stem cells, termed "satellite cells", which are involved in the repair and regeneration of skeletal muscle throughout the lifespan. (josam.org)

While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)
There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
A perivascular origin for mesenchymal stem cells in multiple human organs. (medecinesciences.org)

This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
Postnatal growth and repair of skeletal muscle relies upon a population of quiescent muscle precursor cells, called satellite cells that can be activated to proliferate and differentiate into new myofibers, as well as self-renew to replenish the satellite cell population. (biomedcentral.com)
Pluripotent embryonic stem cells (ESCs) have the unique ability to differentiate into every cell type and to self-renew. (biomedcentral.com)
These monocyte myoblasts proliferate and differentiate, following which, they fuse with existing muscle fibers to form multinucleated myotubes and myofibers. (kosfaj.org)

Most studies investigating satellite cells in vitro use already activated satellite cells, called myoblasts. (sun.ac.za)
Methods: Isolated satellite cells from human muscle biopsies were expanded in vitro creating primary human myoblast (PHM) clones. (sun.ac.za)
To determine their possible roles in tissues and satellite cells in vitro, their expression pattern was examined in tissues from 40-day-old chickens and in satellite cells from the breast muscles of 1-week-old and 2-week-old chickens using RT-PCR and immunocytochemistry analyses. (scielo.br)
Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro. (medecinesciences.org)
Satellite cells are in general easy to isolate from explanted skeletal muscle, and presumably muscle biopsies from an adult mammal would provide an ample supply of cells committed to the myogenic developmental pathway to facilitate the in vitro engineering of functional skeletal muscle. (josam.org)
To study the capacity of satellite cells to form into functional skeletal muscle tissue de novo in culture, we have developed several in vitro tissue models. (josam.org)
Since only small numbers of cells are obtainable from the patient, it requires expansion of cells in vitro . (sciepub.com)
Nevertheless, these primary cells grow slowly in vitro and lose their general characteristics during prolonged culturing period. (sciepub.com)

Embryonic stem cells (ESCs), derived from the blastocyst-stage embryo, are capable of generating all cell types of the mammalian body (pluripotency) and of maintaining the capacity for indefinite self-renewal without compromising their genomic integrity. (biomedcentral.com)

In this report, we show that class I histone deacetylase (HDAC) inhibition, and in particular HDAC8 inhibition, attenuated PKD phosphorylation in skeletal C2C12 myoblasts in response to phorbol ester, angiotensin II and dexamethasone signaling independent of changes in total PKD protein expression. (bvsalud.org)
Gene expression of AMP-activated protein kinase (AMPK), MyoD1, and myogenin, along with the muscle atrophy factors muscle RING finger-1 (MuRF-1) and forkhead box O3a (FoxO3a) upon addition of deer antler extracts to muscle-atrophied C2C12 cells was determined by qRT-PCR after treatment with AICAR. (kosfaj.org)

At the same time, the first three trials using cells derived from pluripotent cells have begun. (biomedcentral.com)

When skeletal muscle is injured due to physical or chemical insult, a pool of self-renewing muscle stem cells residing within the skeletal muscles, called satellite cells, can give rise to differentiated myofibers to repair injured muscle ( Charge & Rudinicki, 2004 Chargé SBP, Rudnicki MA. (scielo.br)
Isolation and culture of individual myofibers and their satellite cells from adult skeletal muscle. (scielo.br)
Transplanted myoblasts can migrate several millimeters to fuse with damaged myofibers in nonhuman primate skeletal muscle. (medecinesciences.org)

Systemic delivery of allogenic muscle stem cells induces long-term muscle repair and clinical efficacy in Duchenne muscular dystrophy dogs. (medecinesciences.org)
In living organisms, if the temperature exceeds the normal range (thermo-neutral zone), it disturbs the normal physiological functioning and induces cell injury. (frontiersin.org)

When activated by injury, satellite cells reenter the cell cycle and proliferate in response to extracellular growth factors ( Relaix & Zammit, 2012 Relaix F, Zammit PS. (scielo.br)

Also, a vascularization strategy using co-culture with endothelial cells (ECs) and fibroblasts improved the survival of the bioengineered skeletal muscle tissues 20 , 21 . (nature.com)
Adult and aged rat myooids had only ~35-60% of the skeletal muscle MHC content of control skeletal muscle from rats, the remaining MHC content consisting of isoforms found in cultured fibroblasts but not in control skeletal muscle. (josam.org)
In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)

Primary satellite cells can be harvested from muscle tissue to investigate or even use as potential therapeutic application. (sun.ac.za)
Satellite cells exist in quiescence in the muscle tissue and only become activated following an insult. (sun.ac.za)
Both miRNAs and siRNAs need to be delivered into the target tissue or cell in order to activate the desired therapeutic effect," Muthiah Manoharan, Ph.D., senior vp, drug discovery, Alnylam Pharmaceuticals, explained. (genengnews.com)
These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
Our work is at the stage of understanding the molecular mechanisms by which the aging of the regenerative potential of organ stem cells can be reversed by particular human embryonic factors that are capable of neutralizing the affects of aged niches on tissue regenerative capacity. (ca.gov)
Thus, the use of hESC-produced pro-regenerative factors for boosting the regenerative capacity of organ stem cells is likely to yield healthy, young tissue. (ca.gov)
Although functional organ stem cells persist in the old, tissue damage invariably overwhelms tissue repair, ultimately causing the demise of an organism. (ca.gov)
More importantly, the bioprinted constructs with neural cell integration facilitate rapid innervation and mature into organized muscle tissue that restores normal muscle weight and function in a rodent model of muscle defect injury. (nature.com)
Skeletal muscle, the most abundant tissue of the body, has remarkable regenerative capacity mainly due to its resident muscle stem cells, also known as satellite cells. (elifesciences.org)
The compromised tissue repair program is attributable to the gradual depletion of stem cells and to altered regulatory signals. (biomed.news)
Adult stem cells play key roles in homeostasis and tissue repair. (biomed.news)
Human Myoblast Genome Therapy (HMGT) is a platform technology of cell transplantation, nuclear transfer, and tissue engineering. (jgc301.com)
Results Histological examination showed survival of lac-z expressing myoblasts in host tissue. (jgc301.com)
These kind of medical grade honey examines are usually mostly tissue-or population-based, while the specific connection is from cell-to-cell. (mrt67307inhibitor.com)
Prior to tissue engineering of functional skeletal muscle from satellite cells harvested from aged mammals, two key questions must be addressed. (josam.org)
The purpose of the current study was to compare the excitability and contractility of myooids engineered from skeletal muscles of 32-month-old rats with that of myooids engineered from adult rat tissue. (josam.org)
Tissue-engineering and cell-based therapies, two prominent branches of Regenerative Medicine, are considered as the most suitable techniques to repair the tissue damage. (sciepub.com)

In another application of HMGT on ischemic cardiomyopathy, the first human myoblast transfer into porcine myocardium revealed that it was safe and effective. (jgc301.com)

Aldehyde dehydrogenase activity promotes survival of human muscle precursor cells. (medecinesciences.org)
WAT is characterised by its capacity to adapt and expand in response to surplus energy through processes of adipocyte hypertrophy and/or recruitment and proliferation of precursor cells in combination with vascular and extracellular matrix remodelling. (springer.com)

A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)
Characterization of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny. (sciepub.com)

For example, when the variant H2AZ is incorporated into the nucleosome it increases the accessibility of DNA to specific transcription factors and promotes the conversion of somatic cells to iPS cells. (jove.com)
Low power laser irradiation (LPLI) promotes proliferation of multiple cells, which (especially red and near infrared light) is mainly through the activation of mitochondrial respiratory chain and the initiation of cellular signaling. (biomedcentral.com)

We've got designed a fresh neuron-to-neuron, serum-free coculture tactic which was utilized to decide the higher-level cell phone peptidome of person primary mammalian nerves. (mrt67307inhibitor.com)

In murine-to-murine transplantation experiments, CXCR4 expression marks a population of adult murine satellite cells with robust engraftment potential in mdx mice, and CXCR4-positive murine muscle-derived SP cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice. (biomedcentral.com)
In this study, we used a canine-to-murine xenotransplantation model to quantitatively compare canine muscle cell engraftment, and test the most effective cell population and modulating factor in a canine model of DMD using allogeneic transplantation experiments. (biomedcentral.com)
Furthermore, the canine-to-murine xenotransplantation model accurately predicted results in canine-to-canine muscle cell transplantation. (biomedcentral.com)
Yet, it remains unclear from murine transplantation experiments which cell population most effectively engrafts into diseased skeletal muscle. (biomedcentral.com)

Indeed, CXCR4-positive muscle-derived side population (SP) cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice [ 10 ]. (biomedcentral.com)
Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity without affecting sarcopenia. (scielo.br)
Transplantation of genetically corrected human iPSC-derived progenitors in mice with limb-girdle muscular dystrophy. (medecinesciences.org)
Myofiber-specific ablation of IRE1α dampens Notch signaling and canonical NF-κB pathway in skeletal muscle of adult mice. (elifesciences.org)

Human primary cultured skeletal myoblasts can directly reflect a patient's metabolic phenotype, because many of the signaling pathways are maintained intact. (zen-bio.com)

The unit can offer higher-throughput systems than typical technologies such as for example atomic drive microscopy and micropipette aspiration [5] and will be used like a comparative tool between different subpopulations of cells. (globaltechbiz.com)
Single-cell RNA sequencing of CVPs derived from hESC lines identified three main progenitor subpopulations. (biolamina.com)

In 2010 beginning of 2011, we have approached the identification and characterization of the proteins that are produced by hESCs and have the rejuvenating and pro-regenerative activity on adult muscle. (ca.gov)
The poor performance of stem cells in an aged organ, such as skeletal muscle, is caused by the changes in regulatory pathways such as Notch, MAPK and TGF‐β, where old differentiated tissues and blood circulation inhibit the regenerative performance of organ stem cells. (ca.gov)

Mesodermal iPSC-derived progenitor cells functionally regenerate cardiac and skeletal muscle. (medecinesciences.org)

Secondly, the quiescent state is an integral part of stem cell regulation, therefore choosing the right protocol for inducing quiescence is important. (sun.ac.za)
Finally, FGF6 and FGF2, both individually and sequentially, were used to treat quiescent myoblasts to determine their involvement in activation and proliferation with the use of cell cycle analysis and mRNA assessment of ki67, p21, myf5, and MyoD. (sun.ac.za)
Unlike FGF2, individual treatment with FGF6 was adequate to activate the quiescent PHMs and aid their re-entry into cell cycle with consistency in all three PHM clones by upregulating ki67 expression. (sun.ac.za)
The inverse sequential treatment order did not demonstrate any significant effect on both activation and proliferation of the quiescent cells. (sun.ac.za)
In Drosophila , a population of muscle-committed stem-like cells called adult muscle precursors (AMPs) keeps an undifferentiated and quiescent state during embryonic life. (biologists.com)
For example, muscle stem cells (MuSCs), located beneath the basal lamina, exist in the quiescent state but can transition to an activated, proliferative state upon injury. (biomed.news)

Intramuscular transplantation of myogenic cells in primates: importance of needle size, cell number, and injection volume. (medecinesciences.org)
Dystrophin expression in muscles of Duchenne muscular dystrophy patients after high-density injections of normal myogenic cells. (medecinesciences.org)
Functional three-dimensional skeletal muscle constructs (myooids) were engineered from myogenic cells harvested from the muscles of adult and aged rats. (josam.org)
Myooids formed by self-organization of myogenic cells in the absence of an artificial scaffold and were maintained in culture. (josam.org)

More research teams are accelerating the use of other types of adult stem cells, in particular neural stem cells for diseases where beneficial outcome could result from either in-lineage cell replacement or extracellular factors. (biomedcentral.com)
Here we show by in vivo fate mapping that brown, but not white, fat cells arise from precursors that express Myf5, a gene previously thought to be expressed only in the myogenic lineage. (bioseek.eu)
Taken together, these data indicate that PRDM16 specifies the brown fat lineage from a progenitor that expresses myoblast markers and is not involved in white adipogenesis. (bioseek.eu)

Ensure high yields when passaging and culturing your primary human skeletal muscle progenitor cells (myoblasts) by using MyoCult™-SF Attachment Substrate, which is optimized to support myoblast attachment. (stemcell.com)
human pluripotent stem cell (hPSC)-derived myoblasts may be cultured on cultureware coated with Corning® Matrigel® hESC-Qualified Matrix. (stemcell.com)
The supplement is combined with a basal medium to prepare MyoCult™-SF Expansion Medium for the culture of primary human and hPSC-derived myoblasts. (stemcell.com)
For more information on protocols for the derivation and expansion of human skeletal muscle progenitor cells with MyoCult™, please explore the Product Information Sheet . (stemcell.com)
Mature human red blood cell (RBC) is perhaps among the simplest cells to model, lacking nucleus and internal cytoskeleton. (globaltechbiz.com)
In the next funding period we also plan to accomplish transition from mouse model to human cells and studies. (ca.gov)
Long-term engraftment (16 years) of myoblasts in a human infarcted heart. (medecinesciences.org)
Pericytes of human skeletal muscle are myogenic precursors distinct from satellite cells. (medecinesciences.org)
Aldehyde dehydrogenase activity identifies a population of human skeletal muscle cells with high myogenic capacities. (medecinesciences.org)
Laminin-211 has also been used for the culture of adult human cardiomyocytes (Kuroda, 2015). (biolamina.com)
ZenBio offers human primary skeletal myoblasts from a variety of donors, including obese donors with Type 2 diabetes. (zen-bio.com)
Human skeletal myoblasts satellite cells are isolated from consented patients with good representation from all levels of adiposity and age. (zen-bio.com)
This strategy allows the particular elucidation regarding cell-specific prohormone processing along with the finding of mobile or portable cellular signaling peptides.Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 can be a mainly food-borne bacterial virus that's competent at creating life-threatening human being infections and also positions a life threatening obstacle in order to public wellness worldwide. (mrt67307inhibitor.com)
Attachment of human marrow stromal cells to titanium surfaces. (sciepub.com)
Osathanon T, Bespinyowong K, Arksornnukit M, Takahashi H, Pavasant P. "Human osteoblast-like cell spreading and proliferation on Ti-6Al-7Nb surfaces of varying roughness. (sciepub.com)

These constructs (mm 3 -cm 3 scale) consisted of tens to hundreds of long parallel myofiber bundles, containing densely packed, highly viable, and aligned muscle cells. (nature.com)
Ex vivo cultures of myofiber explants demonstrate that ablation of IRE1α reduces the proliferative capacity of myofiber-associated satellite cells. (elifesciences.org)
These cells are located beneath the basal lamina of myofiber in a dormant state. (elifesciences.org)

Together, these data suggest that CXCR4 plays an important role in donor cell engraftment. (biomedcentral.com)
We show that CXCR4 expressing cells are important for donor muscle cell engraftment, yet FACS sorted CXCR4-positive cells display decreased engraftment efficiency. (biomedcentral.com)
However, diprotin A, a positive modulator of CXCR4-SDF-1 binding, significantly enhanced engraftment and stimulated sustained proliferation of donor cells in vivo . (biomedcentral.com)
We used a clinically acceptable regimen of hematopoietic stem cell transplantation to establish mixed donor/host blood cell chimerism and immune tolerance in a canine model of Duchenne muscular dystrophy ( cxmd ) [ 9 ]. (biomedcentral.com)
Intramuscular injection of donor muscle-derived cells into chimeric cxmd recipients restored dystrophin expression for at least 24 weeks in the absence of post-transplant immunosuppression, indicating that cell transplantation may be a viable therapeutic option for muscular dystrophy. (biomedcentral.com)
We used the xenotransplant model to show that CXCR4 expression on canine donor muscle cells is also important for cell engraftment, but FACS sorting for CXCR4-positive cells decreased their engraftment efficiency. (biomedcentral.com)
Diprotin A stimulation of CXCR4, however, significantly increased the number of canine dystrophin-positive muscle fibers and canine-derived satellite cells by enhancing donor cell proliferation. (biomedcentral.com)

Transplantation of myogenic stem cells possesses great potential for long-term repair of dystrophic muscle. (biomedcentral.com)
Autologous transplantation of muscle-derived CD133 + stem cells in Duchenne muscle patients. (medecinesciences.org)
Myoblasts cultured from satellite cells of adult muscle biopsies survive, develop, and function to revitalize degenerative muscles upon transplantation. (jgc301.com)

Normalizing for skeletal muscle MHC content and isoform expression predicts myooid forces of ~6-18% of control adult skeletal muscles. (josam.org)

Embryonic myogenic progenitor cells express CXCR4, G-protein coupled cell surface receptor, and migrate towards regions of SDF-1 expression during limb muscle development, suggesting that CXCR4/SDF-1 plays a role in muscle cell homing. (biomedcentral.com)

Bober E, Franz T, Arnold HH, Gruss P, Tremblay P. Pax-3 is required for the development of limb muscles:a possible role for the migration of dermomyotomal muscle progenitor cells. (scielo.br)
Unlike stem cells, myoblasts are differentiated, immature cells destined to become muscles. (jgc301.com)
Myoblasts are safe and efficient gene transfer vehicles endogenous to muscles that constitute 50% of body weight. (jgc301.com)
Myoblast-injected DMD muscles showed improved histology. (jgc301.com)
Because of the decreased proliferation potential [9] and lower absolute number of satellite cells in muscles from the aged rats compared with adult rats [1,10,11] , one may hypothesize that the aged rats would not yield enough myoblasts to produce myooids in culture. (josam.org)

Heparan sulfate proteoglycans (HSPGs) are present on the cell surface and in the extracellular matrix in all metazoans. (scielo.br)
Semaphorins are a wide family of extracellular regulatory signals with a multifaceted role in cell-cell communication. (biomed.news)
Recently, a large number of signaling proteins reported play an important key role in the process of LPLI-induced cell proliferation, probably due to the fact that the molecular events they are involved in are the basic response of the cells to extracellular stimuli. (biomedcentral.com)

We confirmed that typical growth factor signaling was in fact occurring in muscle cells exposed to hESC produced factors, and that hESCs produce a TGF-beta antagonist. (ca.gov)

Yamashita D, Machigashira M, Miyamoto M, Takeuchi H, Noguchi K, Izumi Y, Ban S. "Effect of surface roughness on initial responses of osteoblast-like cells on two types of zirconia. (sciepub.com)
Medium pH modulates matrix, mineral, and energy metabolism in cultured chick bones and osteoblast-like cells. (sciepub.com)

The epidemic of obesity, closely associated with increases in diabetes, hypertension, hyperlipidemia, cancer and other disorders, has propelled a major interest in adipose cells and tissues. (bioseek.eu)
Adipose tissues contain two distinct types of fat cells, white and brown. (bioseek.eu)
The light must be absorbed by the endogenous chromophores of cells or tissues for actions. (biomedcentral.com)

MicroRNA-133 controls brown adipose determination in skeletal muscle satellite cells by targeting Prdm16. (scielo.br)

We demonstrate that mdPCR can give exact molecule counts per cell for mRNA isoforms encoding the myogenic transcription factor Pax3. (biomed.news)
In addition, myooids expressed only developmental isoforms of skeletal muscle MHC, known to generate less specific force than adult isoforms. (josam.org)

Stem cell function, self-renewal, and behavioral heterogeneity of cells from the adult muscle satellite cell niche. (medecinesciences.org)

In skeletal muscle, these are primary satellite cells. (sun.ac.za)
Upon increasing culture times in chicken´s primary skeletal muscle satellite cells, SULF1 and SULF2 expression in 1-week-old chickens was significantly higher than in 2-week-old chickens, suggesting that sulfatases play a key role in satellite cell development. (scielo.br)

The contractile ring components generate the force necessary to divide the cell. (jove.com)

Anatomy65

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