Adult stem adult mouse skeletal muscle skeletal muscle proliferation and differentiation. Medical search. Frequent questions

Still, the existence of these separate "MyoD-dependent" and "Myf5-dependent" subpopulations has been disputed, with some claiming that these MRFs are indeed coexpressed in muscle progenitor cells. (wikipedia.org)
FGF-6 can stimulate migration of myogenic stem cells and also control the maintenance of muscle progenitor cells. (biolegend.com)
Bober E, Franz T, Arnold HH, Gruss P, Tremblay P. Pax-3 is required for the development of limb muscles:a possible role for the migration of dermomyotomal muscle progenitor cells. (scielo.br)
We determined the developmental and functional status of both skeletal muscle progenitor cells as well as adult human satellite cells. (ca.gov)
A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells. (ca.gov)
Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
Skeletal muscle contains a number of different progenitor cells, including satellite and mesenchymal progenitor cells. (hindawi.com)
The goal is to develop a cellular therapy for human diseases either (a) with adult stem/progenitor cells (MSCs), or (b) with therapeutic factors that MSCs produce in response to signals from injured tissues. (tamu.edu)
Ensure high yields when passaging and culturing your primary human skeletal muscle progenitor cells (myoblasts) by using MyoCult™-SF Attachment Substrate, which is optimized to support myoblast attachment. (stemcell.com)
For more information on protocols for the derivation and expansion of human skeletal muscle progenitor cells with MyoCult™, please explore the Product Information Sheet . (stemcell.com)
Is Adipocyte Differentiation the Default Lineage for Mesenchymal Stem/Progenitor Cells after Loss of Mechanical Loading? (scirp.org)
Mesenchymal stem/progenitor cells (MSC/MPC) are found in many tissues and fluids including bone marrow, adipose tissues, muscle, synovial membranes, synovial fluid, and blood. (scirp.org)
Hart, D. (2014) Is Adipocyte Differentiation the Default Lineage for Mesenchymal Stem/Progenitor Cells after Loss of Mechanical Loading? (scirp.org)
Ando, W., Heard, B., Nakamura, N., Frank, C.B. and Hart, D.A. (2012) Ovine Synovial Membrane-Derived Mescenchymal Progenitor Cells Retain the Phenotype of the Original Tissue That Was Exposed to in Vivo Inflammation: Evidence for a Suppressed Chondrogenic Differentiation Potential of the Cells. (scirp.org)
There is a growing body of evidence which demonstrates that following ACS, microRNAs might inhibit fibroblast proliferation and scarring, as well as harmful apoptosis of cardiomyocytes, and stimulate fibroblast reprogramming into induced cardiac progenitor cells. (archivesofmedicalscience.com)
During development, stem cells and resulting progenitor cells are responsible for generating all the tissues and cells of an organism. (wisc.edu)

As one of the key cell markers of satellite cells (the stem cell pool for skeletal muscles), it plays an important role in the regeneration of adult muscle. (wikipedia.org)
Injection of FGF-6 recombinant protein into damaged mouse muscle can induce cyclin D1 mRNA and differentiation markers, including Cdkl1, MHC I and Tn1, which results in the acceleration of muscle regeneration. (biolegend.com)
During muscle regeneration, it has been shown that FGF-6 can be released from necrotic myofibers and be sequestered by basal laminae. (biolegend.com)
Cellular and molecular regulation of muscle regeneration. (scielo.br)
Satellite cells are essential for skeletal muscle regeneration:the cell on the edge returns centre stage. (scielo.br)
Regeneration of muscle after injury normally occurs rapidly with satellite stem cells that reside in the space between the basal lamina and the sarcolemma, playing a major role [ 11 ]. (hindawi.com)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
Regeneration of skeletal muscle is accompanied by the dynamic remodeling of the MuSC niche to promote each step of the regeneration process in a specific manner. (leibniz-fli.de)
They selected this protein because it was among the most prominently affected proteins in skeletal muscle during aging and its level increases progressively with age as well as during regeneration. (leibniz-fli.de)
Satellite cells and skeletal muscle regeneration, Compr. (copernicus.org)
Additionally, we are interested in mechanisms responsible for skeletal muscle recovery and regeneration after injury. (uncg.edu)
More specifically, my laboratory investigates the role inflammation plays in muscle atrophy, as well as during recovery and regeneration after injury. (uncg.edu)
Aged skeletal muscle displays increased fibrosis and impaired regeneration. (uncg.edu)
In aged skeletal muscle, impairments in regrowth and regeneration may be explained by a decreased responsiveness of muscle precursor cells (MPCs) to environmental cues such as growth factors. (uncg.edu)
Skeletal muscle growth and regeneration involves the activity of resident adult stem cells, namely satellite cells (SC). (sdbonline.org)
Collectively, this study provides the first evidence that transient levels of the evolutionarily conserved factor RACK1 are critical for adult SC activation and proper skeletal muscle regeneration, favoring the efficient progression of SC from a committed to a fully differentiated state. (sdbonline.org)
In the adult, stem cells exist in many tissues throughout life and may play critical roles in physiological functions and tissue regeneration. (wisc.edu)
In 2009 beginning of 2010 we have focused on investigating what factors human embryonic stem cells (hESCs) may produce that enhance regeneration and if those factors have any effects by themselves on regeneration. (ca.gov)
Our current running hypothesis is that the positive growth factors produced by hESCs trigger injured muscle to initiate and maintain regeneration, the TGF-beta inhibitors produced by hESCs reduce the TGF-beta signaling, and the combination assures the robust regeneration of muscle. (ca.gov)
Preliminary data indicates the effects on regeneration of old muscle look very promising. (ca.gov)
Specifically, our data suggest that several other ligands of MAPK pathway secreted by hESCs are likely to enhance and rejuvenate the regeneration of old muscle tissue. (ca.gov)
We have submitted the several manuscripts on topics of enhanced tissue regeneration and we are preparing the manuscript that identifies hESC-based novel strategies for restoring high regenerative capacity to old muscle. (ca.gov)
Finally, our data suggest that muscle stem cells either do not accumulate DNA damage with age or can efficiently repair such damage, when activated for tissue regeneration. (ca.gov)
He is developing a treatment strategy for abdominal aortic aneurysms by delivering mesenchymal stem cells or their secreted products to the periadventitial side of the aneurysm to inhibit the matrix degradation commonly seen in the disease progression and promote its regeneration. (pitt.edu)

The miRNA-mRNA profiles and bioinformatics study suggested that the SFRP1 gene was potentially regulated by miRNA-1/206 during porcine skeletal muscle development. (biomedcentral.com)
The expression level of the SFRP1 was highly varied across numerous pig tissues and it was down-regulated during porcine skeletal muscle development. (biomedcentral.com)

These basic helix loop helix transcription factors act sequentially in myogenic differentiation. (wikipedia.org)

It functions during that time to commit myogenic precursor cells to become skeletal muscle. (wikipedia.org)
Furthermore, Myf5 is a master regulator of muscle development, possessing the ability to induce a muscle phenotype upon its forced expression in fibroblastic cells. (wikipedia.org)
While the product of this gene is capable of directing cells towards the skeletal muscle lineage, it is not absolutely required for this process. (wikipedia.org)
Despite only being present for a few days during embryonic development, Myf5 is still expressed in certain adult cells. (wikipedia.org)
Specifically, it allows a brief pulse of proliferation of these satellite cells in response to injury. (wikipedia.org)
The ED 50 is 0.02 - 0.1 ng/mL, corresponding to a specific activity 1.0 - 5.0 x 10 7 units/mg, as determined by a dose-dependent proliferation of NIH3T3 cells in the presence of 1.5 µg/mL heparin. (biolegend.com)
To determine their possible roles in tissues and satellite cells in vitro, their expression pattern was examined in tissues from 40-day-old chickens and in satellite cells from the breast muscles of 1-week-old and 2-week-old chickens using RT-PCR and immunocytochemistry analyses. (scielo.br)
Upon increasing culture times in chicken´s primary skeletal muscle satellite cells, SULF1 and SULF2 expression in 1-week-old chickens was significantly higher than in 2-week-old chickens, suggesting that sulfatases play a key role in satellite cell development. (scielo.br)
When skeletal muscle is injured due to physical or chemical insult, a pool of self-renewing muscle stem cells residing within the skeletal muscles, called satellite cells, can give rise to differentiated myofibers to repair injured muscle ( Charge & Rudinicki, 2004 Chargé SBP, Rudnicki MA. (scielo.br)
Gros J, Manceau M, Thomé V, Marcelle C. A common somitic origin for embryonic muscle progenitors and satellite cells. (scielo.br)
MicroRNA-133 controls brown adipose determination in skeletal muscle satellite cells by targeting Prdm16. (scielo.br)
The proliferation and differentiation of satellite cells are regulated by a number of extracellular signals ( Wang & Rudnicki, 2012 Wang YX, Rudnicki MA. (scielo.br)
Satellite cells, the engines of muscle repair. (scielo.br)
Isolation and culture of individual myofibers and their satellite cells from adult skeletal muscle. (scielo.br)
Günther S, Kim J, Kostin S, Lepper C, Fan CM, Braun T. Myf5-positive satellite cells contribute to Pax7-dependent long-term maintenance of adult muscle stem cells. (scielo.br)
Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity without affecting sarcopenia. (scielo.br)
We are developing a target product profile for human satellite cells derived from human pluripotent stem cells which will be used to use muscle stem cells in personalized medicine for patients with devastating muscle wasting or degenerative diseases. (ca.gov)
We will molecularly and functionally define muscle stem cells in human muscle in development, juvenile and adult and develop strategies to generate the most regenerative muscle stem cells from hPSCs. (ca.gov)
We will perform single cell RNA sequencing (scRNA-seq) of embryonic, fetal, juvenile and adult muscle stem cells (MuSCs) and identify unique gene signatures and cell states across human myogenesis. (ca.gov)
We will identify unique cell surface markers on in vivo derived MuSCs that distinguish PAX7-positive cells from other muscle cells and across cell states. (ca.gov)
in the proliferation and differentiation of mouse embryonic stem cells. (edu.au)
It will also have links through our USA collaborators to understanding the role of such growth factors in the proliferation tumor cells within the brain (U87 a nasty brain tumor). (edu.au)
In particular, this project is aimed at looking at the signalling pathways that are activated when these growth factors activate their receptors, which are on these stem and tumor cells. (edu.au)
Crypts begin to form around day 7 of postnatal mice (P7), and Paneth cells usually appear within the first 2 weeks. (researchsquare.com)
All intestinal epithelial cells (IECs) originate from intestinal stem cells (ISCs) that reside in niches of the lower crypt. (researchsquare.com)
In addition, Paneth cells provide a niche for leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5)-expressing stem cells in crypts (Sato, et al. (researchsquare.com)
Our previous study showed that the absorptive cells of the jejunum and ileum change rapidly and dramatically from suckling to adult type during the weaning period (Fujita, et al. (researchsquare.com)
A number of different local transcription factors called myogenic regulatory factors (MRFs), including MyoD, myf-5, myogenin, and myf-6, stimulate proliferation and differentiation of satellite cells [ 9 ]. (hindawi.com)
In addition, insulin, such as growth factor (IGF) 1Ea and/or IGF-1Ec (MGF), also stimulates proliferation and differentiation of satellite cells. (hindawi.com)
Total and conditional knock out of PPARγ in hair follicle bulge stem cells showed similar result where scarring alopecia was observed in mice with the loss of pilosebaceous units and inflammatory cells infiltration 18 , 19 . (nature.com)
The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
Stem cells are undifferentiated cells which can give rise to a succession of mature functional cells. (justia.com)
Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)
In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
Intrinsic changes in muscle stem cells (MuSCs) alter their proteome in addition to changes in the stem cell niche. (leibniz-fli.de)
By investigating the interplay between changes in the proteome of MuSCs and in their niche, the study published in Cell Reports provides a better understanding of how muscle stem cells are affected during aging. (leibniz-fli.de)
Skeletal muscle has a remarkable ability to regenerate that depends on muscle stem cells (MuSCs) - also termed satellite cells. (leibniz-fli.de)
IL-2/IL2R also promotes the differentiation of T cells into effector T cells. (ilreceptor.com)
Skeletal muscles are the largest cells in the body and are one of the few syncytial ones. (frontiersin.org)
There is a general agreement that hypertrophy is accompanied by the addition of new nuclei from stem cells to help the muscles meet the enhanced synthetic demands of a larger cell. (frontiersin.org)
However, since there are many different non-muscle cell populations that reside within the tissue, these experiments cannot easily distinguish true myonuclei from those of neighboring mononuclear cells. (frontiersin.org)
Skeletal muscle is fairly unique in that the mature cells are syncytial and can contain hundreds of nuclei. (frontiersin.org)
In Drosophila with depleted RACK1 in all muscle cells or, specifically, in SC lineage resulted in a delayed recovery of skeletal muscle after physical damage as well as the low presence of active SC in the wound area. (sdbonline.org)
Our group specializes in determining the cellular and molecular mechanisms of beneficial effects of mesenchymal stem cells (MSCs) in diseases that include heart disease, diabetes, and peritonitis. (tamu.edu)
Adult mesenchymal stem cells (MSCs) represent a subject of intense experimental and biomedical interest. (biomedcentral.com)
Borlongan, C.V., Glover, L.E., Tajiri, N., Kaneko, Y. and Freeman, T.B. (2011) The Great Migration of Bone Marrow-Derived Stem Cells towards the Ischemic Brain: Therapeutic Implications for Stroke and Other Neurological Disorders. (scirp.org)
Batsali, A.K., Kastrinaki, M.C., Papadaki, H.A. and Pontikoglou, C. (2013) Mesenchymal Stem Cells Derived from Wharton's Jelly of the Umbilical Cord: Biological Properties and Emerging Clinical Applications. (scirp.org)
The research in our laboratory focuses on understanding the molecular mechanisms that regulate neural stem cells and neurodevelopment with the goal of applying this knowledge in the treatment of neurological disorders and injuries. (wisc.edu)
Stem cells have two fundamental properties: self-renewal and multipotency. (wisc.edu)
Neural stem cells in the postnatal brain have significant roles in both normal brain functions, such as learning and memory and the brain's response to injuries. (wisc.edu)
My laboratory is investigating the mechanisms governing the behaviors and functions of neural stem cells in both healthy conditions and in neurological diseases. (wisc.edu)
Using neural stem cells as model systems, we are investigating the molecular mechanisms that regulate neuronal development during postnatal period and their implications in human neurodevelopmental disorders such as Rett Syndrome, Autism, and Fragile X syndrome. (wisc.edu)
We confirmed that typical growth factor signaling was in fact occurring in muscle cells exposed to hESC produced factors, and that hESCs produce a TGF-beta antagonist. (ca.gov)
In the next reporting period we will re-confirm that the levels of candidate proteins from the 500 antibody array actually are very highly produced by hESCs and that the signals from these proteins are perceived by regenerating muscle cells. (ca.gov)
Our work is at the stage of understanding the molecular mechanisms by which the aging of the regenerative potential of organ stem cells can be reversed by particular human embryonic factors that are capable of neutralizing the affects of aged niches on tissue regenerative capacity. (ca.gov)
Thus, the use of hESC-produced pro-regenerative factors for boosting the regenerative capacity of organ stem cells is likely to yield healthy, young tissue. (ca.gov)
In the next funding period we also plan to accomplish transition from mouse model to human cells and studies. (ca.gov)
Although functional organ stem cells persist in the old, tissue damage invariably overwhelms tissue repair, ultimately causing the demise of an organism. (ca.gov)
The poor performance of stem cells in an aged organ, such as skeletal muscle, is caused by the changes in regulatory pathways such as Notch, MAPK and TGF‐β, where old differentiated tissues and blood circulation inhibit the regenerative performance of organ stem cells. (ca.gov)
reporter mice had been injected with AAV8-CMV-Cre at postnatal day time 2 (P2) and cells had been analyzed at P10 and P56, respectively. (cancercurehere.com)
No YFP-labeled cells had been recognized in the liver organ of mice treated using the control vector AAV8-CMV-RFP (B). AAV8-CMV-Cre tagged CK19-expressing cholangiocytes and HNF4-expressing hepatocytes (C,D). Yellowish arrowheads: YFP+CK19+ cholangiocytes. (cancercurehere.com)
But according to a review published in Frontiers in Physiology, modern lab techniques now allow us to see that nuclei gained during training persist even when muscle cells shrink due to disuse or start to break down. (robert-gorter.info)
But by far our biggest cells - and biggest syncytia - are our muscles. (robert-gorter.info)
Muscle growth is accompanied by the addition of new nuclei from stem cells to help meet the enhanced synthetic demands of larger muscle cells," explains Schwartz. (robert-gorter.info)
But modern cell-type-specific dyes and genetic markers have shown that the dying nuclei other researchers had detected were in fact inflammatory and other cells recruited to atrophic muscle. (robert-gorter.info)
During adolescence muscle growth is enhanced by hormones, nutrition and a robust pool of stem cells, making it an ideal period for individuals to "bank" myonuclei that could be drawn upon to remain active in old age. (robert-gorter.info)
Promotes neurogenesis in human induced pluripotent stem cells (iPSCs) when used in combination with SB 431542 (Cat. (tocris.com)
DMH-1 induces intestinal differentiation in human intestinal organoids (hIOs) derived from human pluripotent stems cells (hPSCs). (tocris.com)
Here he also utilizes mesenchymal stem cells or their products incorporated in a biodegradable scaffold that undergoes substantial in vivo remodeling to develop a native-like blood vessel. (pitt.edu)
Human mesenchymal stem cells (MSC) with the capacity to differentiate into osteoblasts provide potential for the development of novel treatment strategies, such as improved healing of large bone defects. (biomedcentral.com)
In recent years mesenchymal stem cells (MSC) have generated a great deal of interest as a potential source for cell-based therapeutic strategies. (biomedcentral.com)
We monitored these cells during their expansion ex vivo with respect to proliferation kinetics, surface marker profile and differentiation potential. (biomedcentral.com)
More often than not stem cells are the form of living cells used in this technology. (asu.edu)
Induced Pluripotent Stem Cells (iPSCs) are cells derived from non-pluripotent cells, such as adult somatic cells, that are genetically manipulated so as to return to an undifferentiated, pluripotent state. (asu.edu)
Research on iPSCs, initiated by Shinya Yamanaka in 2006 and extended by James Thompson in 2007, has so far revealed the same properties as embryonic stem cells (ESCs), making their discovery potentially very beneficial for scientists and ethicists alike. (asu.edu)
6,7 Mesenchymal stem cells (MSCs) have also been isolated from other sources, such as bone marrow, 8 the placenta, 9 muscle, 10 or blood. (jcadonline.com)
The bone marrow contains various populations of skeletal stem cells (SSCs) in the stromal compartment, which are important regulators of bone formation. (nature.com)
It is well-described that leptin receptor (LepR) + perivascular stromal cells provide a major source of bone-forming osteoblasts in adult and aged bone marrow. (nature.com)
These Fgfr3-creER -marked endosteal stromal cells contribute to a stem cell fraction in young stages, which is later replaced by Lepr-cre -marked stromal cells in adult stages. (nature.com)
These Fgfr3 + stem/stromal cells with OCT identities are abundant in the young bone marrow and depleted in the old bone marrow, denoting their transitional nature. (nature.com)
Overall, our findings indicate that Fgfr3 + endosteal stem/stromal cells with OCT identities dictate active and aggressive osteogenesis, identifying these cells as an important regulator of long-term bone homeostasis. (nature.com)

Some evidence shows that Myf5 and MyoD are responsible for the development of separate muscle lineages, and are not expressed concurrently in the same cell. (wikipedia.org)
FGF-6 is involved in both proliferation and differentiation of the myogenic lineages. (biolegend.com)
2008). Lgr5 deficiency leads to premature Paneth cell differentiation in the small intestine without detectable effects on the differentiation of other cell lineages, or on proliferation or migration (Garcia, et al. (researchsquare.com)

There is a longstanding belief that a given nucleus controls a defined volume of cytoplasm, so when a muscle grows (hypertrophy) or shrinks (atrophy), the number of myonuclei change accordingly. (frontiersin.org)
Recently, two independent models, one from rodents and the other from insects, have demonstrated that nuclei are not lost from skeletal muscle fibers when they undergo either atrophy or programmed cell death. (frontiersin.org)
Similarly, in preclinical models treated with multiple intramuscular injections of Botulinum Toxin A to induce muscle weakness and atrophy, there is also an infiltration of the muscle with fat. (scirp.org)
This assumption long seemed valid, with many researchers reporting the presence of disintegrating nuclei in muscle tissue during atrophy induced by inactivity, injury or paralysis. (robert-gorter.info)
Muscles get damaged during extreme exercise, and often have to weather changes in food availability and other environmental factors that lead to atrophy. (robert-gorter.info)

DMH-1 suppresses lung cancer cell proliferation, migration, invasion in vitro and reduces tumor growth in a mouse lung cancer xenograft model. (tocris.com)

Li, L. and Jiang, J. (2011) Regulatory Factors of Mesenchymal Stem Cell Migration into Injured Tissues and Their Signal Transduction Mechanisms. (scirp.org)
Tuberous sclerosis complex (TSC) is a genetic disorder affecting cellular differentiation, proliferation, and migration early in development, resulting in a variety of hamartomatous lesions that may affect virtually every organ system of the body. (medscape.com)

It is a protein with a key role in regulating muscle differentiation or myogenesis, specifically the development of skeletal muscle. (wikipedia.org)
In zebrafish, Myf5 is the first MRF expressed in embryonic myogenesis and is required for adult viability, even though larval muscle forms normally. (wikipedia.org)
As no muscle is formed in Myf5;Myod double mutant zebrafish, Myf5 cooperates with Myod to promote myogenesis. (wikipedia.org)
MicroRNA-1/206(miRNA-1/206) is specifically expressed in skeletal muscle and play a critical role in myogenesis. (biomedcentral.com)

During aging the regenerative capacity of skeletal muscle declines, in part due to reduced numbers of MuSCs, but also due to impaired MuSC functionality. (leibniz-fli.de)

Differentiation begins (regulated by other genes) after this initial proliferation. (wikipedia.org)
In each of these mice we have inactivated the genes that encode for synaptic signalling molecules, such as agrin and laminins, or molecules that are needed to cluster ion channels in the postsynaptic membranes of muscles and/or neurones, such as rapsyn and gephyrin. (edu.au)
This project in the first part is simply a promoter and 3' UTR analysis of some 30 genes that appear at the same time in the postsynaptic region of skeletal muscle. (edu.au)
In some cases, "master" genes have been described which control differentiation versus self-renewal. (justia.com)
Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
Panobinostat influences methylation and gene expression pattern in vivo To deal with whether or not panobinostat also influences expres sion of DNMTs and related target genes in vivo, we ana lyzed HepG2 xenograft samples from a previously described nude mouse model. (ilreceptor.com)
Microarray analysis of MSC during osteogenic differentiation identified three candidate genes for further examination and functional analysis: ID4, CRYAB, and SORT1. (biomedcentral.com)

Liver kinase B1 inhibits smooth muscle calcification via high mobility group box 1. (omicsdi.org)

At 8 weeks of age, these mice were injected with tamoxifen to induce deletion of beta cell-specific Atgl (also known as Pnpla2), and the mice were fed an ND or HFD. (omicsdi.org)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)

We also want to know if these signalling pathways activate proliferation and differentiation. (edu.au)
In this review, we focus on the impact of ncRNA post-transcriptional regulatory mechanisms, especially those of microRNAs and lncRNAs, in RA signalling pathways during differentiation and disease. (mdpi.com)
Additionally, we were able to reconstruct the three developmental phases during osteoblast differentiation: proliferation, matrix maturation, and mineralization, and illustrate the activation of the SMAD signaling pathways by TGF-β2 and BMPs. (biomedcentral.com)

Moreover, decreased insulinaemia in B-Atgl-KO mice was associated with increased energy expenditure, and lipid metabolism in brown (BAT) and white (WAT) adipose tissues, leading to reduced fat mass and body weight. (omicsdi.org)
In clinical practice, these involve the life-long immunosuppression of the organ transplantation patients, the creation of the supporting environment for engineered tissues in the mended organ, the unsolved issues of cell survival and differentiation of the cell-based therapy, and the selection and development of vectors for gene therapy. (biomedcentral.com)
GDF11 regulates essential cell differentiation and proliferation responses and is expressed in numerous tissues, including the skin, heart, skeletal muscle , and developing nervous system . (fightaging.org)
MSCs can be isolated from a range of tissues, but the most commonly cited sources are bone marrow (BM), adipose tissue, muscle, bone, and perinatal tissues (e.g. (biomedcentral.com)
What was surprising is that administering these inhibitors to the whole animal appears to reduce TGF-beta levels in the whole animal, suggesting some kind of feed-back and perhaps effects on other tissues as well as muscle. (ca.gov)

Further compounding the loss in function with age is preferential loss of cross-sectional area of stronger and faster contracting type II muscle fibers [ 9 ]. (hindawi.com)
RACK1 was expressed transiently in the skeletal muscle of post-natal mice, being abundant in the early phase of muscle growth and almost disappearing in adult mature fibers. (sdbonline.org)
After acute injury in muscle of both mouse and the fruit fly Drosophila melanogaster (used as alternative in vivo model) this study found that RACK1 accumulated in regenerating fibers while it declined with the progression of repair process. (sdbonline.org)
Until recently, scientists thought this meant that nuclei - the cell control centers that build and maintain muscle fibers - are also lost to sloth. (robert-gorter.info)
Two independent studies - one in rodents and the other in insects - have demonstrated that nuclei are not lost from atrophying muscle fibers, and even remain after muscle death has been initiated. (robert-gorter.info)

FGF-6 regulates muscle differentiation through a calcineurin-dependent pathway and regulates myofiber size through IGF-II/TGF2R pathway. (biolegend.com)

In 2010 beginning of 2011, we have approached the identification and characterization of the proteins that are produced by hESCs and have the rejuvenating and pro-regenerative activity on adult muscle. (ca.gov)

Although it is expressed in both the epaxial (to become muscles of the back) and hypaxial (body wall and limb muscles) portions of the myotome, it is regulated differently in these tissue lines, providing part of their alternative differentiation. (wikipedia.org)
Skeletal muscle is a target tissue for anabolic steroids. (frontiersin.org)
This will involve looking at mice we are making where we the gene for TGFbeta is knockout out in adult muscle and/or in the adult spinal cord (a tissue inducible knockout out of TGFbeta 2). (edu.au)
These results demonstrate that Lkb1 plays an important role in maintaining body weight, liver and adipose tissue function, blood glucose homeostasis and survival in adult mice. (omicsdi.org)
A beta cell ATGL-lipolysis/adipose tissue axis controls energy homeostasis and body weight via insulin secretion in mice. (omicsdi.org)
If, for example, damage to the muscle occurs because of an injury, MuSCs start to divide very quickly and replace the damaged muscle tissue. (leibniz-fli.de)
Skeletal muscle is the largest tissue in the body. (frontiersin.org)
GDF11 was identified in parabiosis studies as beneficially influencing stem cell and tissue function. (fightaging.org)
MSCs can be defined by their ability to adhere to tissue culture plastic, their expression of several cell surface molecular epitopes-cluster of differentiation CD73, CD90, and CD105, and others-as well as their lack of several surface markers, including CD45 [ 6 ]. (biomedcentral.com)

The origins and basis for these fat deposits are largely unknown, but there is a possibility that the altered mechanical and biological environments lead to dysregulation of MSC/MPC and progression to preferential differentiation towards the adipocyte lineage. (scirp.org)

Cellular and molecular mechanisms regulating skeletal muscle development. (scielo.br)
However, the pathophysiological mechanisms underlying this muscle syndrome and its relationship with plasma level of androgens are not completely understood. (frontiersin.org)
This involves exploring the signal transduction mechanisms that are activated when signalling molecules such as neuregulin-1 (ARIA, beta-heregulin 1), or agrin active ErbB or MuSK receptors in the membrane of muscle respectively. (edu.au)
My research focuses on investigating the mechanisms responsible for sarcopenia (the age-related loss of skeletal muscle mass and strength) and developing exercise interventions for the treatment/prevention of sarcopenia. (uncg.edu)
Additionally, our data in progress suggest that muscle and brain age by similar molecular mechanisms and thus, therapeutic strategies for rejuvenating muscle repair might be applicable to the restoration of neurogenesis in aged brain. (ca.gov)
Despite growing experience and knowledge concerning human MSC and their use in cell-based strategies, the molecular mechanisms that govern MSC self-renewal, expansion and multilineage differentiation are not well understood and remain an active area of investigation. (biomedcentral.com)

We have developed methods that allow us to measure thousands of proteins in both, skeletal muscles as well as freshly isolated MuSCs," reports Dr. Svenja Schüler, who led the study as part of her PhD thesis in the Ori lab. (leibniz-fli.de)
In the next step, we focused on the proteins that were significantly affected in both old and geriatric MuSCs and found evidence that they play an important role in metabolic processes and protein homeostasis, as well as influence the communication with the stem cell niche. (leibniz-fli.de)
The analysis of the skeletal muscle proteome revealed close to two hundred extracellular or secreted proteins affected by aging. (leibniz-fli.de)
We were able to assign the majority of ECM proteins that are altered in aging skeletal muscle to at least one distinct cell type," reports Dr. Julia von Maltzahn, research group leader at FLI. (leibniz-fli.de)

To directly assess the role of beta cell lipolysis in insulin secretion and whole-body energy homeostasis, inducible beta cell-specific adipose triglyceride lipase (ATGL)-deficient (B-Atgl-KO) mice were studied under normal diet (ND) and high-fat diet (HFD) conditions. (omicsdi.org)
ND-fed male B-Atgl-KO mice showed decreased insulinaemia and glucose-induced insulin secretion (GSIS) in vivo. (omicsdi.org)
B-Atgl-KO male mice fed an HFD showed reduced insulinaemia, glycaemia in the fasted and fed states and after glucose challenge, as well as enhanced insulin sensitivity. (omicsdi.org)
Decreased insulinaemia due to lowered GSIS protects B-Atgl-KO mice from diet-induced obesity, improves insulin sensitivity, increases lipid mobilisation from WAT and causes BAT activation. (omicsdi.org)
Obesity is associated with lower skeletal muscle capillarization and lower insulin sensitivity. (uncg.edu)

It was shown to inhibit keratinocyte proliferation and promotes terminal differentiation 17 . (nature.com)

mice showed intensified vascular calcification, which was alleviated by treatment with the HMGB1 inhibitor glycyrrhizic acid. (omicsdi.org)
Vascular endothelial growth factor (VEGF) is important for the maintenance of the skeletal muscle capillaries. (uncg.edu)
Recently, we observed that muscle capillarization, vascular endothelial growth factor (VEGF) protein, and the VEGF mRNA response to acute exercise were lower in aged compared with young women. (uncg.edu)

2007). It has been suggested that the intestinal transcription factor Blimp-1 is a critical driver of the postnatal epithelial phenotype, whose expression loss in the third postnatal week is a likely requirement for the maturation of the neonatal epithelium to adult epithelium (Harper, et al. (researchsquare.com)
This is supported by reduced proliferation of human dermal papilla and predominantly epithelial keratinocytes after incubation with AICAR, the agonist for AMPK signaling which activates PPARGC1a and serves as co-activator of PPARγ. (nature.com)

Studies targeted at establishing such effects at cellular level and their correlations with in vivo models, will broaden our understanding of the roles played by androgens on skeletal muscle function in elderly. (frontiersin.org)
In this process, MuSCs interact with their immediate cellular environment, also known as the stem cell niche. (leibniz-fli.de)
It has also been suggested that GDF11 is involved in the age-related global physiological decline in function, and that restoring circulating blood levels of GDF11 could reverse some of the cellular and physiological dysfunctions observed in aged mice. (fightaging.org)

This work could provide a cell therapy for muscle disorders including muscular dystrophies or sarcopenia. (ca.gov)
A large majority of California residents are affected or will be affected with a muscle disease in their lifetime including severe muscular dystrophies, aging related muscle loss called sarcopenia or muscle weakness. (ca.gov)
Sarcopenia, the age-related loss of skeletal muscle mass and function, is becoming more prevalent as the lifespan continues to increase in most populations. (frontiersin.org)
Testosterone concentrations decline as age increase, suggesting that low plasma testosterone levels can cause or accelerate muscle- and age-related diseases, as sarcopenia. (frontiersin.org)
Currently, there is increasing interest on the anabolic properties of testosterone for therapeutic use in muscle diseases including sarcopenia. (frontiersin.org)
This age-related decline in skeletal muscle mass and strength generation, the primary function of skeletal muscle mass, is known as sarcopenia ( 2 , 3 ). (frontiersin.org)
Indeed, most of the intrinsic as well as extrinsic (systemic) muscle changes that occur with age are believed to be involved in the development of sarcopenia ( 5 , 6 ). (frontiersin.org)
The term sarcopenia was first proposed in 1989 by Irwin Rosenberg to describe a multifactorial syndrome that occurs with age and results in loss of skeletal muscle mass and function ( 3 , 4 ). (frontiersin.org)
Significant loss of muscle mass may occur in cachexia and sarcopenia, which are major causes of mortality and disability. (mdpi.com)
These adverse changes in muscle quantity, muscle quality, and the resulting functional deficits are referred to under a common name of "sarcopenia. (hindawi.com)
4 ] used the relative skeletal muscle mass index which is calculated by normalizing appendicular skeletal muscle mass, measured by dual-energy X-ray absorptiometry, to height squared in meters to diagnose sarcopenia. (hindawi.com)
The European Working Group on Sarcopenia in Older People (EWGSOP) recommends diagnosis of sarcopenia based on the presence of low muscle mass and low muscle strength or low physical performance [ 5 ]. (hindawi.com)
For the purposes of this paper, we define sarcopenia as originally defined by Rosenberg [ 3 ], an age-related loss of muscle, and view the functional aspects of the most recent definitions as consequences of sarcopenia. (hindawi.com)
As well, many older individuals suffer from sarcopenia, a protracted muscle wasting disorder that typically begins after the age of 50 and involves a loss of about 1% of muscle mass per year ( Woo, 2017 ). (frontiersin.org)

It has been noted that in humans and preclinical animal models that exposure to microgravity/space flight or prolonged bed rest (a surrogate for microgravity) can lead to infiltration of skeletal muscle and bone marrow with fat. (scirp.org)

Androgens are the main physiologic anabolic steroid hormones and normal testosterone levels are necessary for a range of developmental and biological processes, including maintenance of muscle mass. (frontiersin.org)
However, before we can gain a proper understanding of their role in mice that are missing these ion channel subunits, we need to know their developmental expression profile in the developing mouse. (edu.au)
Hiroyuki Koike is an Associate Professor in the Department of Medical Biochemistry, Tokyo Medical and Dental University, specializing in stem cell biology and developmental biology. (tmd.ac.jp)

Inhibition of H3K27 trimethylation suppressed crypt formation and Paneth cell maturation on organoids derived from ileum of early second postnatal mouse. (researchsquare.com)
The expression level of the SFRP1 was significantly higher in the embryonic skeletal compared with postnatal skeletal muscle, whereas miR-206 showed the inverse pattern of expression. (biomedcentral.com)
Meat science and muscle biology symposium: stem cell niche and postnatal muscle growth, J. Anim. (copernicus.org)
Consequently, we treated postnatal day time 2 (P2) mice with AAV8-CMV-Cre and livers had been examined at P10 (Shape 1A). (cancercurehere.com)

The dynamics of RACK1 levels in isolated adult SC of mice, i.e., progressively high during differentiation and low compared to proliferating conditions, and RACK1 silencing indicated that RACK1 promotes both the formation of myotubes and the accretion of nascent myotubes. (sdbonline.org)

The biological part of this project will involve making transgenic mice with test non-coding DNA linked to reporter constructs such as green fluorescent protein (GFP). (edu.au)

Clinical studies of androgen supplementation in age-related diseases and muscle wasting are a focus of emerging interest ( 11 ). (frontiersin.org)
Although serum GDF11 levels were found to be decreased in old mice, supplementation with GDF11 "rejuvenated" them, thereby suggesting that GDF11 is a key player in mammalian life span. (fightaging.org)

human pluripotent stem cell (hPSC)-derived myoblasts may be cultured on cultureware coated with Corning® Matrigel® hESC-Qualified Matrix. (stemcell.com)
Myoblasts cultured using MyoCult™-SF Expansion Medium can be differentiated into multinucleated myotubes using MyoCult™ Differentiation Kit (Human) . (stemcell.com)

FGF-6 mRNA can also be detected in testis, heart, and skeletal muscle in adults. (biolegend.com)
Circular transcripts of the testis-determining gene Sry in adult mouse testis, Cell, 73, 1019-1030, https://doi.org/10.1016/0092-8674(93)90279-y, 1993. (copernicus.org)

Develop transcription factor over-expression in combination with improved directed differentiation strategies to generate MuSCs from hPSCs with genetic and functional signatures of the in vivo MuSCs. (ca.gov)
The maintenance of their "stemness" state and commitment to differentiation are tightly controlled by both intrinsic genetic and epigenetic programs and extrinsic stimuli. (wisc.edu)

Gene expression of Hanwoo satellite cell differentiation in longissimus dorsi and semimembranosus, Bmc Genomics, 20, 156, https://doi.org/10.1186/s12864-019-5530-7, 2019. (copernicus.org)

To address this question we first developed human oligonucleotide microarrays with 30.000 elements and then performed large-scale expression profiling of long-term expanded MSC and MSC during differentiation into osteoblasts. (biomedcentral.com)

As constitutive knockout of Lkb1 in mice leads to embryonic lethality, whether Lkb1 is required for the growth and survival of adult mice is unclear. (omicsdi.org)

It has been shown that the expression of FGF-6 can transiently be upregulated by muscle injuries in both fast and slow myofibers. (biolegend.com)

Cell Stem Cell 2013;13(5):590-601. (scielo.br)

Taken together, we identified an aging signature of the ECM that is conserved between different skeletal muscles supporting the notion that the MuSC niche is progressively remodeled with increasing age in different muscles of the body. (leibniz-fli.de)

While acknowledging progress in the other areas mentioned, this work will focus on the current debates concerning sourcing, MSC alterations of angiogenesis, cell differentiation/stimulation, and strategies to improve MSC differentiation. (biomedcentral.com)

These and other data argue against the current interpretation of the myonuclear domain hypothesis and suggest that once a nucleus has been acquired by a muscle fiber it persists. (frontiersin.org)

As such, the findings have important implications beyond understanding muscle biology. (robert-gorter.info)

Anatomy66

Organisms17

Diseases14

Chemicals and Drugs67

Analytical, Diagnostic and Therapeutic Techniques and Equipment32

Phenomena and Processes59

Disciplines and Occupations4

Anthropology, Education, Sociology and Social Phenomena2

Technology, Industry, Agriculture1

Information Science3

Health Care1

Progenitor cells16

Regeneration24

Porcine skeletal muscle2

Myogenic differentiation1

Cells91

Lineages3

Atrophy5

Vitro1

Migration2

Myogenesis4

Aging the regenerative capacity1

Genes7

Inhibits1

Induce2

Pathways3

Tissues5

Fibers5

Regulates1

Regenerative1

Tissue9

Lineage1

Mechanisms6

Proteins4

Insulin5

Terminal differentiation1

Vascular3

Epithelial2

Cellular3

Sarcopenia14

Bone1

Developmental3

Postnatal4

Promotes1

Transgenic mice1

Supplementation2

Myoblasts2

Testis2

Genetic2

Genomics1

Osteoblasts1

Knockout1

Transiently1

Cell Stem Cell1

Progressively1

Angiogenesis1

Hypothesis1

Findings1