Loading...
  • donors
  • This study will establish efficient protocols for generating iDA neurons through direct conversion of adult fibroblasts from healthy donors as well as people with idiopathic or genetic forms of Parkinson s disease. (michaeljfox.org)
  • On Nov. 21, 2005, on the eve of screning of a documentary by MBC network in Korea, Roh Sung-Il, a senior co-author of Hwang's and head of a Seoul fertility clinic (MizMedi Hospital), admitted compensating 16 donors of the ova used in Hwang's stem cell research. (climateaudit.org)
  • Production of cloned pigs by using somatic cells as donors. (springer.com)
  • Human
  • Human adult fibroblast-derived DA neurons will be tested for their ability to have an excitable membrane, establish synapses and produce and release dopamine. (michaeljfox.org)
  • Following is the text of Seoul National University's report on Dr. Hwang Woo Suk, the South Korean researcher who claimed to have cloned human cells. (nytimes.com)
  • The investigation on the 2004 Science paper in which the establishment of the first human ES cell line from cloned blastocyst was reported was initiated in response to various doubts raised on photographs of the cells and results from DNA fingerprinting analyses. (nytimes.com)
  • Although there are indications that human adult heart has certain level of endogenous regeneration capacity, with different estimations of the rate of cardiomyocyte turnover between studies, adult human heart cannot effectively regenerate after injury [ 3 - 6 ]. (hindawi.com)
  • Theoretically, de novo cardiomyocytes for cell replacement therapy could potentially solve the problem of availability of human cardiac tissue. (hindawi.com)
  • The paper not only seemed to validate the group's claim a year earlier that it had created a single cell line from a cloned human embryo, but it also reported a huge increase in efficiency for the technique. (climateaudit.org)
  • After Hwang and his colleagues announced in 2004 that they had derived stem cells from a cloned human embryo, a news article in Nature a couple of months later quoted a Ph.D. student from Hwang's lab saying that she and another junior colleague donated eggs for the experiments. (climateaudit.org)
  • This study is the first to use human iPS cells to generate electricall. (bio-medicine.org)
  • This study is the first to use human iPS cells to generate electrically active motor neurons, a key hallmark of functional maturation that is essential for any future application of iPS cells. (bio-medicine.org)
  • Led by William Lowry, and in collaboration with Bennett Novitch, Harley Kornblum, and Martina Wiedau-Pazos of the University of California Los Angeles, researchers compared the ability of different human cell lines to generate motor neuron progenitors and fully differentiated motor neurons. (bio-medicine.org)
  • It seems possible that disease-specific somatic cells may be reprogrammed and utilized to model, and ultimately to treat a variety of human neurological disorders," says Miodrag Stojković, co-editor of the journal. (bio-medicine.org)
  • Further oxidation of this position is required for normal mitochondrial translation and function in human somatic cells. (springer.com)
  • neurons
  • Through this procedure, it will be, then, possible to facilitate and accelerate the production of disease-affected iDA neurons for studying in the dish multiple pathogenetic mechanisms of PD with relevant patient s cells. (michaeljfox.org)
  • In addition, iDA neurons will be explored as a superior source for cell replacement therapeutic approaches in PD in order to restore a dopaminergic neuronal network competent to re-establish dopamine function when necessary. (michaeljfox.org)
  • To our knowledge, our results present the first demonstration of the electrical activity of iPS-derived neurons and further suggest the feasibility of using these cells to explore how changes in motor neuron activity contributes to the degeneration of these cells underlying these disorders," the authors state. (bio-medicine.org)
  • When measuring the electrophysical properties of the iPS-derived neurons, the researchers found that the iPS cells followed a normal developmental progression to mature, electrically active neurons. (bio-medicine.org)
  • stem cell
  • In conclusion, the research team of Professor Hwang does not possess patient-specific stem cell lines or any scientific bases for claiming having created one. (nytimes.com)
  • Cell Stem Cell 1:502-512. (koreascience.or.kr)
  • In October 2005, Seoul National University (SNU), where Hwang was a professor, launched the World Stem Cell Hub, headed by Hwang. (climateaudit.org)
  • A few weeks later, South Korea's JoongAng Ilbo newspaper reported that Schatten had asked Hwang for a half share in the patent for patient-specific stem cell cloning six weeks before his withdrawal from the project. (climateaudit.org)
  • He said he did so without informing Woo Suk Hwang, the high-profile stem cell biologist who used the eggs in his research and has been embroiled in controversy in the 10 days since his American collaborator broke off relations and charged the Korean team with unethical behavior. (climateaudit.org)
  • viable
  • Studies in amphibians, rabbits, and mice suggested that the very early cleavage stages (two-cell to four-cell) were flexible and that each blastomere could yield a viable blastocyst. (pnas.org)
  • These findings support the possibility that reprogrammed somatic cells might prove to be a viable alternative to embryo-derived cells in regenerative medicine," the authors note. (bio-medicine.org)
  • mesenchymal cells
  • A) LacZ-expressing cardiomyocytes of neonatal rats were cocultured with GFP+ bone marrow mesenchymal cells. (nih.gov)
  • GFP+ mesenchymal cells (a, arrow) expressed cTnT (b, arrow) and β-gal (c, arrow) in the same cell (merged on d). (nih.gov)
  • Proliferation
  • In particular, we are asking two questions: (1) how fate controls the pace of proliferation and (2) how cells find their proper migration paths and destination during gastrulation/morphogenesis. (mskcc.org)
  • Hwang's
  • The DNA samples for the ES cell line included those from 20 subcultured NT-1 cell lines in culture or in frozen state from Professor Hwang's laboratory, one deposited to the Korean Cell Line Bank for the purpose of securing a patent, one maintained in Professor Shin Yong Moon's laboratory at SNU, and one maintained in the MizMedi Hospital. (nytimes.com)
  • Among the twenty independent subcultures from Professor Hwang's laboratory, nine produced the identical pattern to the aforementioned three samples, but the other eleven produced a distinct pattern that was in fact identical to the fingerprinting pattern of MizMedi ES cell line #5 derived from IVF egg. (nytimes.com)
  • While the fingerprinting pattern of the anonymous donor A, the source of the somatic and egg cells according to Professor Hwang's team, was identical to what was reported in the Science article, it was clearly different from that of NT-1 line. (nytimes.com)
  • Korean Times reported that, in June 2005, an investigative reporting program of MBC, a major broadcaster in South Korea, received a tip that there is a chance that Hwang's team had violated ethics codes and his 2005 paper in Science on tailormade stem cells is fraudulent. (climateaudit.org)
  • blastocyst stage
  • Prior to the blastocyst stage, cells in the early embryo, called blastomeres, divide without increasing in mass between each division: thus the term cleavage divisions-each cell cleaves in half. (pnas.org)
  • ESCs
  • In contrast to monolayer cultures, however, the spheroid structures that are formed when ESCs aggregate enables the non-adherent culture of EBs in suspension, making EB cultures inherently scalable, which is useful for bioprocessing approaches, whereby large yields of cells can be produced for potential clinical applications. (wikipedia.org)
  • subsequent
  • The stem cells that Professor Hwang claims to have created subsequent to the 2005 publication were also turned out to have originated from frozen fertilized eggs and not from cloned blastocysts. (nytimes.com)
  • organ
  • Molecular analyses of these phenomena are hindered by the fact that testis is a complex organ with many types of intimately intermingled somatic and germline cells. (biomedcentral.com)
  • 2016
  • In 2016 scientists reported no defects in thirteen cloned sheep, including four from the same cell line as Dolly. (wikipedia.org)
  • genes
  • We sorted out 829 genes predominantly expressed from the germinal part and 944 genes from the somatic part of the testis. (biomedcentral.com)
  • We created a catalogue of genes expressed in the adult mouse testis of the C57BL/6J (abbreviated here B6) inbred strain. (biomedcentral.com)
  • The production of Dolly showed that genes in the nucleus of such a mature differentiated somatic cell are still capable of reverting to an embryonic totipotent state, creating a cell that can then go on to develop into any part of an animal. (wikipedia.org)
  • fluorescence
  • The main cell types can be fractionated, via gravity sedimentation, centrifugal elutriation or fluorescence activated cell sorting, but the time required can be fairly long to exclude possible artificial changes of mRNA levels. (biomedcentral.com)
  • An embryoid body whose cells differentiated into enhanced green fluorescence protein -expressing spontaneously beating cardiomyocytes . (wikipedia.org)