Adult satellite cells express myogenic factor pax7 transcription factor. Medical search. Frequent questions

The transcription factor CCAAT/enhancer binding protein beta (C/EBPβ) is expressed in Pax7 + satellite cells of healthy muscle and is downregulated during myoblast differentiation. (biomedcentral.com)
Persistent expression of C/EBPβ upregulates Pax7, inhibits MyoD, and blocks myogenic differentiation. (biomedcentral.com)
CCAAT/enhancer binding proteins (C/EBPs) are a family of transcription factors that regulate cellular growth and differentiation, metabolism, and inflammation [ 18 ]. (biomedcentral.com)
When overexpressed in the murine myoblast cell line C2C12 or in primary myoblasts, C/EBPβ reduced MyoD and other myogenic protein levels during differentiation, in addition to reducing myoblast fusion. (biomedcentral.com)
Activated satellite cells initially proliferate as skeletal myoblasts before undergoing myogenic differentiation. (wikipedia.org)
However, Pax7 is expressed prominently after satellite cell differentiation. (wikipedia.org)
These dividing cells are known as the "transit amplifying pool" before undergoing myogenic differentiation to form new (post-mitotic) myotubes. (wikipedia.org)
One of the first roles described for IGF-1 was its involvement in the proliferation and differentiation of satellite cells. (wikipedia.org)
Their fate is regulated by the expression of various transcription factors, including paired box 7 (Pax7) and myogenic differentiation (MyoD) [ 6 ]. (biomedcentral.com)
Using synchronized cultures of C2C12 myoblasts to model these divergent programs, we show that p8 (also known as Nupr1 ), a G1-induced gene, negatively regulates the cell cycle and promotes myogenic differentiation. (biologists.com)

Greater activation also results in increased expression of myogenic basic helix-loop-helix transcription factors MyoD, myogenin, and MRF4 - all responsible for the induction of myocyte-specific genes. (wikipedia.org)
In the resected muscle and in the passively stretched antagonist muscle, there was a dramatic increase in the number of myofibers positive for neonatal MyHC and in the number of BrdU- and MyoD-positive satellite cells. (arvojournals.org)

More specifically, upon activation, satellite cells can re-enter the cell cycle to proliferate and differentiate into myoblasts. (wikipedia.org)

In addition, IGF-1 expression in skeletal muscle extends the capacity to activate satellite cell proliferation (Charkravarthy, et al. (wikipedia.org)
Proliferating, BrdU-positive cells in muscle sections were detected with immunohistochemistry indicating different cell types and decreasing proliferation with age. (nature.com)
Cell culture experiments indicated that Gln could promote cell proliferation in a dose dependent manner, but expression of myogenesis regulatory genes was not altered. (nature.com)
Overall, Gln supplementation stimulated cell proliferation in muscle tissue and in vitro in myogenic cell culture, whereas muscle growth regulatory genes were barely altered. (nature.com)
6 These manipulations result in satellite cell activation and proliferation, which in turn are responsible for muscle repair and regeneration. (arvojournals.org)
A single, early local administration of high-dose bFGF prevented atrophic changes in the thyroarytenoid muscles by activating satellite cell proliferation and reforming neuromuscular junctions. (biomedcentral.com)
Hepatocyte growth factor (HGF) is produced by stromal and mesenchymal cells, and it stimulates epithelial cell proliferation, motility, morphogenesis and angiogenesis in various organs via tyrosine phosphorylation of its cognate receptor, Met. (spandidos-publications.com)
WAT is characterised by its capacity to adapt and expand in response to surplus energy through processes of adipocyte hypertrophy and/or recruitment and proliferation of precursor cells in combination with vascular and extracellular matrix remodelling. (springer.com)

By contrast, transforming growth factor (TGF)-β1 was demonstrated to markedly downregulate HGF gene expression ( 10 , 11 ). (spandidos-publications.com)

Understanding the effects of strabismus surgery on muscle cell biological reactions and myofiber remodeling may suggest new approaches for improving surgical outcomes. (arvojournals.org)
There are a number of factors that initiate myofiber remodeling in adult skeletal muscles. (arvojournals.org)

Using genetic tools to conditionally abrogate C/EBPβ expression in Pax7 + cells, we examined the role of C/EBPβ in self-renewal of satellite cells during muscle regeneration. (biomedcentral.com)
After a single muscle injury, C/EBPβ-deficient satellite cells fail to self-renew resulting in a reduction of satellite cells available for future rounds of regeneration. (biomedcentral.com)
These findings demonstrate that C/EBPβ is a novel regulator of satellite cell self-renewal during muscle regeneration acting at least in part through Notch2. (biomedcentral.com)
These cells represent the oldest known adult stem cell niche, and are involved in the normal growth of muscle, as well as regeneration following injury or disease. (wikipedia.org)
1 2 Residing outside the sarcolemma but within the basal lamina of individual myofibers is a population of cells that are responsible for muscle repair and regeneration, the satellite cells. (arvojournals.org)
Satellite cells (SCs), which are precursors of skeletal muscles, such as the TA muscle, play a key role in muscle homeostasis and regeneration. (biomedcentral.com)
SCs are normally in a quiescent state in adult muscles, and start proliferating during muscle regeneration in response to denervation. (biomedcentral.com)
Conditional Met KO mice were generated using Cre‑loxP methodology and characterization of these mice indicated that the HGF‑Met signaling pathway is essential in regeneration, protection, and homeostasis in various tissue types and cells. (spandidos-publications.com)

The basic fibroblast growth factor (bFGF) is a member of the fibroblast growth factor family and is a key protein in regenerative medicine. (biomedcentral.com)
To further define which neurons express Eph, antibodies were generated to the cytoplasmic portion of the Eph protein. (sdbonline.org)

Adult regenerative myogenesis is dependent on progenitor cells. (biologists.com)

Both active and passive stretch of the rectus muscles produced by strabismus surgery dramatically upregulated the processes of satellite cell activation, integration of new myonuclei into existing myofibers, and concomitant upregulation of immature myosin heavy chain isoforms. (arvojournals.org)
It is considered postmitotic, in that the myonuclei residing within these myofibers do not divide in adult muscle. (arvojournals.org)

Myocyte nuclear factor (MNF), and c-met proto-oncogene (receptor for hepatocyte growth factor (HGF)) are less commonly used markers. (wikipedia.org)
Hepatocyte growth factor (HGF). (spandidos-publications.com)

Postnatal growth and repair of skeletal muscle relies upon a population of quiescent muscle precursor cells, called satellite cells that can be activated to proliferate and differentiate into new myofibers, as well as self-renew to replenish the satellite cell population. (biomedcentral.com)

Various growth factors, cytokines, and prostaglandins upregulate HGF gene expression, including basic fibroblast growth factor, oncostatin M, hypoxia-inducible factor 1α and nuclear factor-κB (NF-κB) ( 9 ). (spandidos-publications.com)

HGF is produced and secreted by adjacent stromal and mesenchymal cells, it contributes to the development of epithelial organs in a paracrine fashion, exerts regenerative effects on epithelia in the liver, kidney, lung, and other tissues, and promotes the regression of fibrosis in numerous organs ( 7 , 8 ). (spandidos-publications.com)

Satellite cells are precursors to skeletal muscle cells, able to give rise to satellite cells or differentiated skeletal muscle cells. (wikipedia.org)

Satellite cells express a number of distinctive genetic markers. (wikipedia.org)

10 Even more compelling is a recent study that subjected individual quiescent satellite cells isolated from adult limb muscle to stretching in vitro, and showed that direct stretching of the satellite cells themselves results in activation and bromodeoxyuridine (BrdU) incorporation. (arvojournals.org)

Research on regenerative medicine using basic fibroblast growth factor (bFGF) has recently advanced in the field of laryngology. (biomedcentral.com)

Therefore, we complemented our investigations into the cellular development of the skeletal muscle of neonatal piglets with in vitro studies using a primary porcine myogenic cell culture model with Gln supplementation. (nature.com)

Nuclear factor of activated T-cell (NFAT. (biologists.com)

There is also evidence suggesting that these cells are capable of fusing with existing myofibers to facilitate growth and repair. (wikipedia.org)
Satellite cells proliferate following muscle trauma and form new myofibers through a process similar to fetal muscle development. (wikipedia.org)
3 When a muscle is injured, this specialized cell population becomes activated, divides, and either repairs damaged fibers or forms new myofibers. (arvojournals.org)

Myosatellite cells are located between the basement membrane and the sarcolemma of muscle fibers, and can lie in grooves either parallel or transversely to the longitudinal axis of the fibre. (wikipedia.org)
Satellite cells are able to differentiate and fuse to augment existing muscle fibers and to form new fibers. (wikipedia.org)

Immunohistochemistry indicated that bFGF significantly increased the number of satellite cells in the thyroarytenoid muscle up to day 14 and that of neuromuscular junctions on days 28 and 56. (biomedcentral.com)

for example, greater activation results in the progressive loss of Pax7 expression as they enter the proliferative stage. (wikipedia.org)
In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)

Jyotsna Dhawan ABSTRACT Adult stem cells persist in mammalian tissues by entering a state of reversible quiescence, referred to as G 0 , which is associated with low levels of transcription. (biologists.com)
Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)

The insertional 6 mm of the superior rectus muscles of adult rabbits were resected and reattached to the original insertion site. (arvojournals.org)
Irradiation treatment, which eliminates satellite cells from these muscles, prevents the compensatory hypertrophy in these types of experiments. (arvojournals.org)

On the other hand, activated satellite cells have an increased number of caveolae, cytoplasmic organelles, and decreased levels of heterochromatin. (wikipedia.org)

They become activated and re-enter the cell cycle. (wikipedia.org)

eph dsRNA was injected into syncytial stage embryos to perturb eph expression at the larval time points relevant to axon targeting in the adult visual system. (sdbonline.org)

CD34 and Myf5 markers specifically define the majority of quiescent satellite cells. (wikipedia.org)

In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)

The delayed growth of LBW piglets is mainly due to a slower development of skeletal muscle as the myogenic activity is usually impaired in these animals during the fetal and early postnatal period 3 , 4 . (nature.com)

Studies rely almost exclusively on Flow cytometry and fluorescence activated cell sorting (FACS) analysis, which gives no information about cell lineage or behaviour. (wikipedia.org)

In healthy young adults, the skeletal muscle mass comprises approximately 60% of total body mass. (frontiersin.org)

Thousands of copies of the circular mtDNA are present in most cell types that are packaged by TFAM into higher-order structures called nucleoids1. (regenerativemedicine.net)

HGF was cloned as a growth factor for hepatocytes ( 1 , 2 ), is identical to scatter factor (SF) and was originally discovered as a fibroblast-derived cell motility factor for epithelial cells ( 3 ). (spandidos-publications.com)

In response to mechanical strain, satellite cells become activated. (wikipedia.org)

Myosatellite cells, also known as satellite cells, muscle stem cells or MuSCs, are small multipotent cells with very little cytoplasm found in mature muscle. (wikipedia.org)

The field of satellite cell biology suffers from the same technical difficulties as other stem cell fields. (wikipedia.org)

Eph continues to be expressed in the larval CNS and imaginal discs, as well as pupal and adult stages as assayed by Northern blot analysis. (sdbonline.org)

Anatomy11

Organisms4

Chemicals and Drugs33

Analytical, Diagnostic and Therapeutic Techniques and Equipment6

Phenomena and Processes24

Disciplines and Occupations1

Information Science4

Differentiation10

MyoD2

Myoblasts1

Proliferation8

Downregulate1

Myofiber2

Regeneration8

Protein2

Progenitor cells1

Myonuclei2

Hepatocyte growth2

Proliferate and differentiate1

Upregulate1

Promotes1

Precursors1

Genetic1

BrdU1

Regenerative1

Vitro1

Nuclear1

Myofibers3

Fibers2

Immunohistochemistry1

Activation2

Tissues2

Muscles2

Cytoplasmic1

Cycle1

Expression1

Define1

Addition1

Activity1

Studies1

Healthy1

Types1

Growth1

Response1

Small1

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