• That is what New Jersey legislators did when they passed and then Governor James McGreevey signed S-1909 last year, a law that was sold to the public as outlawing human cloning but which actually permits the creation of cloned human life, and its implantation and gestation up to and including the very moment prior to the emergence of the cloned baby from the birth canal. (cbc-network.org)
  • Some prohibit only cloning for reproductive purposes and allow the creation of cloned human embryos for research, whereas others prohibit the creation of cloned embryos for any purpose. (who.int)
  • The artificial cloning of organisms, sometimes known as reproductive cloning, is often accomplished via somatic-cell nuclear transfer (SCNT), a cloning method in which a viable embryo is created from a somatic cell and an egg cell. (wikipedia.org)
  • In 1996, Dolly the sheep achieved notoriety for being the first mammal cloned from a somatic cell. (wikipedia.org)
  • Dolly the Sheep, the first mammal to be cloned from an adult derived somatic cell, was born in 1996. (nottingham.ac.uk)
  • The primary cloning technique is called "somatic cell nuclear transfer" (SCNT). (cbc-network.org)
  • It is the policy of Washington state that research involving the derivation and use of human embryonic stem cells, human embryonic germ cells, and human adult stem cells from any source, including somatic cell nuclear transplantation , is permitted upon full consideration of the ethical and medical implications of this research. (cbc-network.org)
  • Somatic-cell nuclear transfer, the technique by which Dolly was created, was first used 40 years ago in research with tadpoles and frogs. (who.int)
  • The nucleus of an adult somatic cell (such as a skin cell) is removed and transferred to an enucleated egg, which is then stimulated with electric current or chemicals to activate cell division. (who.int)
  • if it implants and the pregnancy goes to term, the resulting individual will carry the same nuclear genetic material as the donor of the adult somatic cell. (who.int)
  • Scientists were initially interested in somatic-cell nuclear transfer as a means of determining whether genes remain functional even after most of them have been switched off as the cells in a developing organism assume their specialized functions as blood cells, muscle cells, and so forth. (who.int)
  • 체세포 핵 치환 (Somatic-cell nuclear transfer, SCNT)은 난자 의 핵 을 제거한 후에, 체세포 의 핵을 이식하여 복제 를 하는 기술을 말한다. (wikipedia.org)
  • In contrast, Dolly was produced by what's called somatic cell nuclear transfer. (wptv.com)
  • Dolly demonstrated that adult somatic cells also could be used as parents. (wptv.com)
  • By my calculations, Dolly was the single success from 277 tries at somatic cell nuclear transfer. (wptv.com)
  • Sometimes the process of cloning by somatic cell nuclear transfer still produces abnormal embryos, most of which die. (wptv.com)
  • Professor Campbell was instrumental in the creation of Dolly the Sheep, the first cloned mammal, a breakthrough which paved the way for the successful cloning of many other mammal species. (nottingham.ac.uk)
  • His pioneering studies into cell-cycle control and cellular differentiation led to the programme of work at Roslin that gave birth to the first mammal to be cloned from adult cells - ie. (nottingham.ac.uk)
  • It's been 20 years since scientists in Scotland told the world about Dolly the sheep , the first mammal successfully cloned from an adult body cell. (wptv.com)
  • If it is to be brought to birth, the process is usually called "reproductive cloning. (cbc-network.org)
  • Elaboration of an international convention against reproductive cloning of human beings has been under consideration in the United Nations since December 2001 when the subject was included in the agenda of the fifty- sixth session as a supplementary agenda item at the request of France and Germany. (who.int)
  • Canovas S, Ross PJ, Kelsey G, Coy P. DNA Methylation in Embryo Development: Epigenetic Impact of ART (Assisted Reproductive Technologies). (animal-reproduction.org)
  • The second way to reproduce is a strictly human invention - known as "asexual" reproduction - or more commonly, cloning. (cbc-network.org)
  • Cloning of a human being" means asexual reproduction by implanting or attempting to implant the product of nuclear transplantation [e.g., an embryo] into a uterus or substitute for a uterus with the purpose of producing a human being. (cbc-network.org)
  • This is junk biology since implanting isn't the act of asexual reproduction: SCNT cloning is. (cbc-network.org)
  • He then moved to PPLTherapeutics, the company that was spun out from Roslin Institute, where that procedure and his expertise led to the birth of cloned and genetically modified sheep, pigs and cattle. (nottingham.ac.uk)
  • As part of its charge, the committee was asked to prepare a subreport evaluating methods for detecting potential unintended compositional changes across the spectrum of messenger ribonucleic acid (mRNA), proteins, metabolites and nutrients that may occur in food derived from cloned animals that have not been genetically modified via genetic engineering methods. (nationalacademies.org)
  • The use of embryonic stem cells, which can be produced through SCNT, in some stem cell research has attracted controversy. (wikipedia.org)
  • Inevitably most people will remember him for Dolly the sheep although his recent work was focused on fundamental and applied stem cell research as a tool for the study of human disease. (nottingham.ac.uk)
  • The bill purports to promote stem-cell research, while outlawing the cloning of a human being. (cbc-network.org)
  • While stem-cell research holds enormous potential for treating or even curing some diseases, the cloning of a human being is morally and ethically unacceptable…Any attempt to clone a human being is in direct conflict with the public policies of this state. (cbc-network.org)
  • Dolly was an important milestone, inspiring scientists to continue improving cloning technology as well as to pursue new concepts in stem cell research. (wptv.com)
  • If artificial cloning and natural cloning both lead to the same result, which is the formation of a clone, that is, an organism with identical or nearly identical genes to another organism, then the plight of This creation is very different between the two creatures. (wikipedia.org)
  • On the other hand, a chimera is defined as an organism in which cells from two or more different organisms have contributed. (frontiersin.org)
  • At that point - and this is important to understand - there is no more cloning to be done since a new human organism now exists. (cbc-network.org)
  • If the cloned human organism is to be experimented upon and destroyed, the process is often called "therapeutic cloning. (cbc-network.org)
  • If the authors of this bill really meant what they appear to have written, their legislation would ban all human cloning, since as we have seen, biologically, a new human organism, that is, a new human being, comes into existence with the completion of SCNT. (cbc-network.org)
  • Or to put it the other way around, cloning, not implantation, is what produces a new and distinct human organism. (cbc-network.org)
  • Occasionally, the term cloning is misleadingly used to refer to the identification of the chromosomal location of a gene associated with a particular phenotype of interest, such as in positional cloning. (wikipedia.org)
  • Via positional cloning, we show that mutations in a zebrafish LTCC encoding gene, cav1.3a , underlie the auditory-vestibular defects of gemini ( gem ) circler mutants. (jneurosci.org)
  • Professor Campbell was a cell biologist/embryologist with a research career spanning more than 30 years, the majority of which was in the field of cell growth and differentiation. (nottingham.ac.uk)
  • NT3 is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. (origene.com)
  • In addition, TFs generally initiate and guide cell fate such as lineage progression and control the stability of cell differentiation [ 5 ]. (biomedcentral.com)
  • However, a number of other features are needed, and a variety of specialised cloning vectors (small piece of DNA into which a foreign DNA fragment can be inserted) exist that allow protein production, affinity tagging, single-stranded RNA or DNA production and a host of other molecular biology tools. (wikipedia.org)
  • I've been working with mammalian embryos for over 40 years, with some work in my lab specifically focusing on various methods of cloning cattle and other livestock species. (wptv.com)
  • Other recent studies verified the presence of PAPP-A mRNA in granulosa cells of humans, monkeys, cattle, mice, and pigs. (bioone.org)
  • Following two postdoctoral positions he joined the Roslin Institute in Scotland in 1991, where he applied his previous experience to the production of mammalian embryos by nuclear transfer. (nottingham.ac.uk)
  • The advent of techniques to propagate animals by nuclear transfer, also known as cloning, potentially offers many important applications to animal agriculture, including reproducing highly desired elite sires and dams. (nationalacademies.org)
  • It may occur accidentally in the case of identical twins, which are formed when a fertilized egg splits, creating two or more embryos that carry almost identical DNA. (wikipedia.org)
  • In sexual reproduction, clones are created when a fertilized egg splits to produce identical (monozygous) twins with identical genomes. (who.int)
  • These germ cells are the only ones in the body that have their genetic material all jumbled up and in half the quantity of every other kind of cell. (wptv.com)
  • NANOG is a core transcription factor (TF) in embryonic stem cells (ESCs) and primordial germ cells (PGCs). (biomedcentral.com)
  • Although NANOG plays a key role in germ cells, the molecular mechanism underlying its transcriptional regulation in PGCs has not been studied. (biomedcentral.com)
  • A germ cell-specific gene regulatory network is required to maintain the unique properties of primordial germ cells (PGCs) for transmission of genetic information to the next generation [ 6 ]. (biomedcentral.com)
  • It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. (origene.com)
  • The sodium current underlying action potentials in guinea pig hippocampal CA1 neurons. (xenbase.org)
  • It is, however, important to distinguish the use of bST from other biotechnologies, such as transgenic or cloned animals. (nationalacademies.org)
  • In order to investigate the effect of hLZ-containing milk on the inflammatory response, young pigs were fed pasteurized milk from hLZ or non-transgenic control goats and quantitative real-time PCR was performed to assess local expression of TNF-α, IL-8, and TGF-β1 in the small intestine. (shengsci.com)
  • We generated a transgenic (Tg) mouse line expressing Cre recombinase under the control of the Gpr88 promoter within a bacterial artificial chromosome clone. (shengsci.com)
  • Transgenic expression of prothymosin alpha on zebrafish epidermal cells promotes proliferation and attenuates UVB-induced apoptosis. (shengsci.com)
  • His research blossomed after he came to Roslin Institute where in a series of papers he put the intellectual framework into the method of mammalian cloning that ultimately led to the birth of Dolly in 1996. (nottingham.ac.uk)
  • Although many species produce clonal offspring in this fashion, Dolly, the lamb born in 1996 at a research institute in Scotland, was the first asexually produced mammalian clone. (who.int)
  • What was special about Dolly is that her "parents" were actually a single cell originating from mammary tissue of an adult ewe. (wptv.com)
  • Dolly was an exact genetic copy of that sheep - a clone. (wptv.com)
  • In fact, one of the coauthors of the paper announcing Dolly worked in our laboratory for three years prior to going to Scotland to help create the famous clone. (wptv.com)
  • Dolly was the culmination of hundreds of cloning experiments that, for example, showed diploid embryonic and fetal cells could be parents of offspring. (wptv.com)
  • It produces malignancy in human and newborn hamster kidney cell cultures. (lookformedical.com)
  • In the field of biotechnology, cloning is the process of creating cloned organisms of cells and of DNA fragments. (wikipedia.org)
  • Detailed descriptions of methods used in animal cloning and biotechnology are provided in the report Animal Biotechnology: Science-Based Concerns (NRC, 2002). (nationalacademies.org)
  • Natural cloning occurs through a variety of natural mechanisms, from single-celled organisms to complex multicellular organisms. (wikipedia.org)
  • Most natural cloning occurs in those species that produce their descendants asexually, that is, without combining the male and female genetic material. (who.int)
  • Increases in the amount of PAPP-A mRNA in granulosa cells during follicular development occurs in some but not all species, indicating that other proteases or protease inhibitors may be involved in IGFBP degradation. (bioone.org)
  • Cloning is the process of producing individual organisms with identical genomes, either by natural or artificial means. (wikipedia.org)
  • The term applies not only to entire organisms but also to copies of molecules (such as DNA) and cells. (who.int)
  • There are now two ways to create new mammalian life, including humans. (cbc-network.org)
  • The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE , infecting B-cells in humans. (lookformedical.com)
  • Another example of artificial cloning is molecular cloning, a technique in molecular biology in which a single living cell is used to clone a large population of cells that contain identical DNA molecules. (wikipedia.org)
  • Molecular cloning refers to the process of making multiple molecules. (wikipedia.org)
  • Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response. (lookformedical.com)
  • Cell adhesion molecules: implications for a molecular histology. (academicinfluence.com)
  • Cell adhesion and the molecular processes of morphogenesis. (academicinfluence.com)
  • Specific molecular sites or structures on or in cells with which interferons react or to which they bind in order to modify the function of the cells. (lookformedical.com)
  • However, though BC is emerging as a potential organ transplant option, challenges regarding organ size scalability, immune system incompatibilities, long-term maintenance, potential evolutionary distance, or unveiled mechanisms between donor and host cells remain. (frontiersin.org)
  • Many studies have investigated germ cell-specific gene promoters to understand their regulatory mechanisms. (biomedcentral.com)
  • depictions commonly involve themes related to identity, the recreation of historical figures or extinct species, or cloning for exploitation (i.e. cloning soldiers for warfare). (wikipedia.org)
  • Cloning is commonly used to amplify DNA fragments containing whole genes, but it can also be used to amplify any DNA sequence such as promoters, non-coding sequences and randomly fragmented DNA. (wikipedia.org)
  • Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
  • Mares were killed 7.5-8.5 days after transfer and the uterus and oviducts flushed for embryo recovery. (bioone.org)
  • Examples of a novel series of phenanthrolinones are shown to be potent competitive inhibitors of avian prolyl 4-hydroxylase, and of collagen hydroxylation, in embryonic chick tendon cells and human foreskin fibroblasts in vitro and in the oestradiol-stimulated rat uterus in vivo. (embl.de)
  • With preconception genetic counseling, this does not put embryos at risk, you could diagnose serious diseases. (diyhpl.us)
  • It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES. (lookformedical.com)
  • instead of half the genetic material coming from a sperm and half from an egg, it all comes from a single cell. (wptv.com)
  • Here, we aimed to characterize DMRs previously identified in embryos, in the blood and sperm of adult progenies of two groups of heifers (low S/Co and control). (animal-reproduction.org)
  • They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation. (lookformedical.com)
  • Histological changes were also investigated, specifically looking at villi width, length, crypt depth, and lamina propria thickness along with cell counts for intraepithelial lymphocytes and goblet cells. (shengsci.com)
  • Significantly higher expression of anti-inflammatory cytokine TGF-β1 was seen in the ileum of pigs fed pasteurized milk containing hLZ (P = 0.0478), along with an increase in intraepithelial lymphocytes (P = 0.0255), and decrease in lamina propria thickness in the duodenum (P = 0.0001). (shengsci.com)
  • In addition to antiviral activity, it activates NATURAL KILLER CELLS and B-LYMPHOCYTES , and down-regulates VASCULAR ENDOTHELIAL GROWTH FACTOR expression through PI-3 KINASE and MAPK KINASES signaling pathways. (lookformedical.com)
  • Cloning has been proposed as a means of reviving extinct species. (wikipedia.org)
  • it's highly variable, though, depending on the cell type used and the species. (wptv.com)
  • The present report gives an overview of the terms and methods used in cloning and summarizes the debates in the General Assembly. (who.int)
  • In addition, the committee was charged with evaluating methods to detect potential, unintended, adverse health effects of foods derived from cloned animals. (nationalacademies.org)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
  • Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
  • She lived to six and a half years, when she was eventually put down after a contagious disease spread through her flock, infecting cloned and normally reproduced sheep alike. (wptv.com)
  • The term "clone", from the Greek word for twig, denotes a group of identical entities. (who.int)
  • Thus, the clone would be genetically identical to the nucleus donor only if the egg came from the same donor or from her maternal line. (who.int)
  • Beyond this scientific interest, the commercial concern in animal cloning focuses on replicating large numbers of genetically identical animals, especially those derived from a progenitor that has been modified genetically. (who.int)
  • When the one-cell embryo duplicates its genetic material, both cells of the now two-cell embryo are genetically identical. (wptv.com)
  • When they in turn duplicate their genetic material, each cell at the four-cell stage is genetically identical. (wptv.com)
  • Even while clones are genetically identical, their phenotypes - the characteristics they express - will be different. (wptv.com)
  • These proteins have been implicated in control of viral transcription and may possibly serve as the "glue" that binds the nucleocapsid to the appropriate membrane site during viral budding from the host cell. (lookformedical.com)
  • Proteins secreted by vertebrate cells in response to a wide variety of inducers. (lookformedical.com)
  • Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. (lookformedical.com)
  • This pioneering study has helped pave the way for others to develop gene and stem-cell based strategies for therapeutic purposes. (nottingham.ac.uk)
  • The nucleus of a body cell from the DNA donor is removed, and put into the place formerly occupied by the egg's nucleus. (cbc-network.org)
  • However, an animal created through this technique would not be a precise genetic copy of the source of its nuclear DNA because each clone derives a small amount of its DNA from the mitochondria of the egg (which lie outside the nucleus) rather than from the donor of cell nucleus. (who.int)
  • In this process, researchers remove the genetic material from an egg and replace it with the nucleus of some other body cell. (wptv.com)
  • PHOSPHORYLATION of IRF-3 causes the protein to be translocated from the CYTOPLASM to CELL NUCLEUS where it binds DNA , and activates transcription. (lookformedical.com)
  • There he continued his research on the cloning and genetic modification of livestock. (nottingham.ac.uk)
  • From that moment forward, nearly all cells in that body have the same genetic makeup. (wptv.com)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • Based on these results we conclude that consuming pasteurized milk containing hLZ does not induce an inflammatory response and improves the health of the small intestine in pigs. (shengsci.com)
  • In a previous study, we found that low levels of sulphur and cobalt (low S/Co) in the diet offered to oocyte donors altered the DNA methylome of bovine embryos. (animal-reproduction.org)
  • Therefore, due to the highly dynamic epigenetic state during early embryonic development, we suggest that is essential to validate the DMRs found in embryos in adult individuals. (animal-reproduction.org)
  • Regulation of the NANOG gene by TFs, epigenetic factors, and autoregulatory factors is well characterized in ESCs, and transcriptional regulation of NANOG is well established in these cells. (biomedcentral.com)
  • The fact that the DNA of a fully differentiated (adult) cell could be stimulated to revert to a condition comparable to that of a newly fertilized egg and to repeat the process of embryonic development demonstrates that all the genes in differentiated cells retain their functional capacity, although only a few are active. (who.int)
  • PGCs express several pluripotency-related TFs such as NANOG, POU5F3 , and SOX2 , and their expression controls transcription of germness-related genes in these cells [ 11 , 29 ]. (biomedcentral.com)
  • [12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
  • Coined by Herbert J. Webber, the term clone derives from the Ancient Greek word κλών (klōn), twig, which is the process whereby a new plant is created from a twig. (wikipedia.org)
  • In 1913, E. Steinhardt, C. Israeli, and R. A. Lambert grew vaccinia virus in fragments of guinea pig corneal tissue. (wikipedia.org)
  • A little shot of electricity comes next, and if all goes well, a new human cloned embryo comes into being and begins to develop in the same way as a sexually created embryo. (cbc-network.org)
  • In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
  • It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. (lookformedical.com)
  • and altering cell and tissue characteristics for biomedical research and manufacturing. (nationalacademies.org)
  • Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
  • After the cells of interest have been isolated from living tissue , they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
  • They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. (lookformedical.com)
  • To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)
  • from nationalreview.com Let's call it "stealth human-cloning legalization. (cbc-network.org)
  • It's easy to do: First, write a proposed law that you claim outlaws human cloning. (cbc-network.org)
  • your supposed cloning ban actually authorizes human cloning, implantation, and gestation through the ninth month. (cbc-network.org)
  • And now Washington joins the infamous list with Senate Bill 5594, a thoroughly disingenuous piece of legislation that purports to outlaw the cloning of human beings, but by manipulating language and redefining terms, actually permits human cloning and gestation of the resulting cloned embryos through the ninth month. (cbc-network.org)
  • They are different uses for the cloned human lives created via cloning. (cbc-network.org)
  • It defines the term "cloning of a human being" inaccurately. (cbc-network.org)
  • Moreover, while the term "human being" is not defined in the legislation, in this context, it can only mean the birth of a cloned baby. (cbc-network.org)
  • To date, some 35 countries have adopted laws forbidding human cloning. (who.int)
  • L-type Ca 2+ channels (LTCCs) drive the bulk of voltage-gated Ca 2+ entry in vertebrate inner ear hair cells (HCs) and are essential for mammalian auditory processing. (jneurosci.org)
  • Adult and embryonic Brugia malayi are shown to be susceptible to inhibitors of vertebrate prolyl 4-hydroxylase, with exposed parasites exhibiting pathologies consistent with a disruption in cuticle biosynthesis. (embl.de)
  • This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • In bioethics, there are a variety of ethical positions regarding the practice and possibilities of cloning. (wikipedia.org)
  • Expressed sequence tag (EST) analysis and developmental polymerase chain reaction (PCR) studies demonstrated that Ov-phy-1 was expressed in L3 and adult parasites. (embl.de)
  • They confer resistance against many different viruses, inhibit proliferation of normal and malignant cells, impede multiplication of intracellular parasites, enhance macrophage and granulocyte phagocytosis, augment natural killer cell activity, and show several other immunomodulatory functions. (lookformedical.com)
  • Cell density regulates prolyl 4-hydroxylase activity independent of mRNA levels. (embl.de)
  • That way when these so-called haploid cells come together at fertilization, they produce one cell with the full complement of DNA. (wptv.com)
  • Proinsulin C-peptide is known to bind specifically to cell membranes and to exert intracellular effects, but whether it is internalized in target cells is unknown. (researchgate.net)
  • Since the term entered the popular lexicon in a more general context, the spelling clone has been used exclusively. (wikipedia.org)
  • Therefore, identification of regulatory elements within the promoter region is considered crucial to understand the mechanism underlying transcriptional regulation in specific cell types. (biomedcentral.com)
  • Therefore, regulation of NANOG expression plays a critical role in determining the fate of pluripotent cells. (biomedcentral.com)
  • Alternative sites for cell density regulation of the enzyme could rely on its sensitivity to sulfhydryl groups. (embl.de)
  • More than 10 different cell types have been used successfully as "parents" for cloning. (wptv.com)
  • Established cell cultures that have the potential to propagate indefinitely. (lookformedical.com)
  • Chemical characterization of a neural cell adhesion molecule purified from embryonic brain membranes. (academicinfluence.com)