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  • regenerative
  • To assess regenerative potential, human CDCs were injected into the border zone of acute myocardial infarcts in immunodeficient mice, with histological examination and echocardiographic left ventricular function as endpoints. (ahajournals.org)
  • Clonal long-term expansion of primary adult liver stem cells opens up experimental avenues for disease modeling, toxicology studies, regenerative medicine, and gene therapy. (uu.nl)
  • HEALTHeUNIVERSE Inc. is engaged in research, development and commercialization of regenerative medicine therapies utilizing non-embryonic adult stem cells for use in plastic and reconstructive surgery, orthopedic surgery, vascular surgery, cardiac surgery, and wound care. (marketwired.com)
  • cardiac
  • Cardiac myocytes have been traditionally regarded as terminally differentiated cells that adapt to increased work and compensate for disease exclusively through hypertrophy. (ahajournals.org)
  • Recent studies have even surmised the existence of resident cardiac stem cells, endothelial cells generating cardiomyocytes by cell contact or extracardiac progenitors for cardiomyocytes, but these findings are still controversial. (ahajournals.org)
  • Adult cardiac muscle, unlike skeletal muscle, lacks the ability to regenerate after ischemic injury, and death of cardiomyocytes promotes a cascade that results in heart failure. (ahajournals.org)
  • Over the past decade, cardiac cellular transplantation techniques have made significant gains, and a variety of cell types have been proposed as useful candidates (see review 1 ). (ahajournals.org)
  • 12 Recently, Bittner et al 13 showed that bone marrow-derived cells could be recruited to both skeletal and cardiac muscle in the mdx mouse, in which these striated muscles undergo continual remodeling. (ahajournals.org)
  • therapy
  • As any disorder involving loss of, or injury to, normal cells could be a candidate for stem cell replacement therapy, the potential of stem cells is profound. (mja.com.au)
  • Stem cell therapy for the nervous system has generated particular interest because of the debilitating nature and widespread occurrence of neurodegenerative disorders. (mja.com.au)
  • 1 These cells may be a candidate for cell-replacement therapy for nervous system disorders. (mja.com.au)
  • This study provides preclinical evidence for the future use of human DPSCs in cell therapy to improve outcome in stroke patients. (curehunter.com)
  • He then decided to consult Dr Pradeep Mahajan to be treated by stem cell therapy. (stem-cells-news.com)
  • fibroblasts
  • We propose it would be more desirable to induce expandable neural precursor cells directly from human fibroblasts. (pubstemcell.com)
  • This study represents a novel virus-free approach for direct reprogramming of human fibroblasts to a neural precursor fate. (pubstemcell.com)
  • [ 14-18 ] We therefore investigated whether adult human fibroblasts could be directly reprogrammed to become neural precursor cells through ectopic expression of specific neural transcription factors using non-viral technologies. (pubstemcell.com)
  • Here, we demonstrate the generation of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. (ceek.jp)
  • These findings demonstrate that iPS cells can be generated from adult human fibroblasts. (ceek.jp)
  • lineages
  • Inductions of the cells into multiple mesenchymal lineages resulted in the expression of several lineage-specific genes, proteins and specific metabolic activity. (h-brs.de)
  • embryos
  • The issue of stem cell research is politically and ethically charged, as so much emphasis has been placed on the use of stem cells derived from early human embryos. (mja.com.au)
  • Scientists have for the first time grown embryos that contain a combination of pig and human stem cells, in a step toward one day growing transplantable organs, a study said on Thursday. (stem-cells-news.com)
  • grown
  • Thirdly, about 80% of the stem cells grown in the muscle media differentiated into smooth muscle cells. (webwire.com)
  • Percutaneous endomyocardial biopsy specimens from 70 adult patients grown in primary culture developed multi-cellular clusters known as cardiospheres, which were plated to yield cardiosphere-derived cells (CDCs). (ahajournals.org)
  • Interestingly, Dr. Iacovitti notes, some of the cellular markers that would be expected to be expressed by new bone marrow cells were present in bone marrow stem cells grown in the original monolayers, though they were fewer in number. (blogspot.com)
  • bone marro
  • Neil Scolding, professor of clinical neurosciences for North Bristol NHS Trust, who is leading the trial, said: "We believe that bone marrow cells have the capability to repair precisely the type of damage that we see in the brain and spinal cord in MS.So by giving patients very large numbers of their own bone marrow cells we hope that this will help stabilise the disease and bring about some repair. (bioethics.com)
  • 9 When bone marrow cells from a normal male mouse were injected intravenously into the tail vein of affected mdx female mice, skeletal myotubes in the recipients were found to contain Y-positive nuclei. (ahajournals.org)
  • ii) History of Stem Cell Research Over 30 years ago, similar "uncommitted and untrained" bone marrow cells were discovered in animals ( and subsequently in adult humans) which had the potential of forming blood components including red and white corpuscles, blood clotting and immune factors and any other haemopoietic function required. (studyres.com)
  • Progenitors
  • After plating the cells at 1 to 1,000 cells/cm 2 , the cultures underwent a time-dependent transition from early progenitors, defined as thin, spindle-shaped cells (RS-1A), to wider, spindle-shaped cells (RS-1B), and to still wider, spindle-shaped cells (RS-1C). (wiley.com)
  • Distinct gene expression program dynamics during erythropoiesis from human induced pluripotent stem cells compared with adult and cord blood progenitors. (ox.ac.uk)
  • We hypothesised that substantial differential regulation of gene expression during erythroid development accounts for these important differences between hiPSC-derived cells and those from adult or cord-blood progenitors. (ox.ac.uk)
  • Whilst the dynamics of erythroid program elaboration from adult and cord blood progenitors were very similar, the emerging erythroid transcriptome in hiPSCs revealed radically different program elaboration compared to adult and cord blood cells. (ox.ac.uk)
  • Endogenous
  • Human and porcine CDCs co-cultured with neonatal rat ventricular myocytes cycled calcium in sync with neighboring myocytes, demonstrated nodal- and ventricular-like action potentials, exhibited endogenous inward sodium currents, inwardly rectifying potassium currents, and L-type calcium currents when transduced with the β-subunit of the L-type calcium channel. (ahajournals.org)
  • Endogenous morphogens and fibrin bioscaffolds for stem cell therapeutics. (uni-bielefeld.de)
  • Prochymal
  • The characteristics and biologic activity of Prochymal®, along with a good safety profile in human trials to date, suggest that Prochymal® may be a good candidate for addressing this unmet medical need. (clinicaltrials.gov)
  • Protocol 260 - Subjects will be randomized with equal probability to the treatment arms (2 million cells/kg of Prochymal or 8 million cells/kg of Prochymal) using a stratified block design. (knowcancer.com)
  • clinical
  • Major hurdles remain to be overcome before human clinical trials can be embarked upon. (mja.com.au)
  • Because these cells also have been isolated and expanded from human heart biopsy specimens, they could have a significant impact on future clinical strategies to treat patients with heart disease. (ahajournals.org)
  • However, the PD plus patients did not show any change in their clinical status after stem cell transplantation. (hindawi.com)
  • Resources and Clinical Issues : i) Criteria for the Use of Stem Cells They must be capable of self renewal and of producing the same or different progeny. (studyres.com)
  • As such, somatic cell reprogramming provides a unique platform by which to dissect the molecular mechanisms that underlie epigenetic reprogramming and lineage specification as well as the exciting opportunity to personalize human cells for both research and clinical application. (pubstemcell.com)
  • Culture bag systems for clinical applications of adult human neural crest-derived stem cells. (uni-bielefeld.de)
  • melanocytes
  • For example, after 14 days, 20% to 40% of the cells in the melanocyte media took on a weblike shape typical of melanocytes. (webwire.com)
  • Also in the basal layer few melanocytes with melanin pigments in the cells cytoplasm were found.The 20-day old cultured epithelium had 8-10 layers consisting of small and round basal cells, scquamous cells and 2-3 layers of keratinized cells. (ac.ir)
  • biopsy
  • The biopsy was cut into thin pieces and treated with trypsin at 4° C overnight (cold trypsin method) to obtain a single-cell suspension. (ac.ir)
  • novel
  • RESULTS: Our high resolution transcriptional view of definitive erythropoiesis captures the regulation of genes relevant to cell-cycle control and confers statistical power to deploy novel bioinformatics methods. (ox.ac.uk)
  • genes
  • HiPSCs show reduced expression of c-KIT and key erythroid transcription factors SOX6, MYB and BCL11A, strong HBZ-induction, and aberrant expression of genes involved in protein degradation, lysosomal clearance and cell-cycle regulation. (ox.ac.uk)
  • methods
  • Methods and Results - A small bone marrow aspirate was taken from the iliac crest of healthy human volunteers, and hMSCs were isolated as previously described. (ahajournals.org)
  • of leukaemia and other haematological malignancies, we still need research into : (i) methods of culture using Cytokines and stimulatory growth factors, (ii) readily available sources of stem cells (eg. (studyres.com)
  • neuronal
  • After 14 days, about 10% of the stem cells in the neuronal cell line -- a type of cell not present in skin or hair -- grew dendrites, the long extensions typical of nerve cells and expressed neuronal proteins. (webwire.com)
  • Phase
  • Primary cultures were usually subcultured when the cells were in exponential growth phase .Colonies of keratinocytes were expanded and after 7-10 days fused and formed a coherent stratified epithelium. (ac.ir)
  • membrane
  • The presence of lipid molecules, including cholesterol in particular, in the plasma membrane plays a crucial role in regulating a variety of physiological processes in cells. (springer.com)
  • Findings from this study will contribute to the understanding of how cholesterol/CAV-1/caveolae regulates aspects of the cell membrane important to cell adhesion, substrate sensing, and microenvironment interaction. (springer.com)
  • The membrane fluidity in the five experimental cell groups were measured using pyrene fluorescence probe staining followed by FACS analysis. (springer.com)