Adult cells 2000 clone cells. Medical search. Frequent questions

However, there are a number of factors limiting the procurement of organs and accordingly, therapeutic cloning that perhaps can yield still better results needs to be considered as an alternative. (scialert.net)
Otherwise, such a treaty would not recognize the inherent human nature of the early human embryo or fetus until after birth , and thus cloning them and using them for research - both "therapeutic" and "reproductive" -- would not be banned, and women undergoing "infertility treatments" could surely be put in danger. (lifeissues.net)
At just 31 years old, Hochedlinger has already worked on therapeutic cloning in a mouse model ( 2 ), reprogramming cancer nuclei ( 3 ), and the molecular mechanisms controlling stem cell pluripotency ( 4 ). (rupress.org)
Human cloning ought to be banned, both reproductive cloning and so-called therapeutic cloning-or as Stanford University recently referred to it, "human nuclear transplantation. (probe.org)
The Donaldson committee 1 examined the issue of research into 'therapeutic' cloning and reported in June 2000. (cmq.org.uk)
The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)
In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
However, the therapeutic utility of UCB HSPC has been limited to pediatric patients due to the low cell frequency per unit of UCB. (bvsalud.org)

In 1997, Ritchie and Campbell in collaboration with PPL (Pharmaceutical Proteins Limited) created another sheep named "Polly", created from genetically altered skin cells containing a human gene. (wikipedia.org)
Note: Please read The Little Lamb That Made a Monkey of Us All for the author's comments on the news of a successful lamb cloning (March 7, 1997). (probe.org)
Dr. Bohlin was named a Research Fellow of the Discovery Institute's Center for the Renewal of Science and Culture in 1997, 2000 and 2012. (probe.org)
In 1997, Lou Hawthorne began a quest to clone Missy, his mother's beloved collie-husky cross. (newscientist.com)
BACKGROUND: Acellular pertussis (aP) vaccines replaced whole cell pertussis (wP) vaccines for the U.S. childhood primary series in 1997. (cdc.gov)

In the recourse of FOXP3, RUNX1 means computer of these tumors( Shevach 2000, Maloy and Powrie 2001, Sakaguchi 2004, Ono et al. (erik-mill.de)
The former CIHR Guidelines for Human Pluripotent Stem Cell Research (2002, 2005, 2006, 2007, and 2010), the Report of the ad hoc working group (2002) and Discussion paper (2001) are available in electronic format on request. (gc.ca)
Species and cultivars of Ceanothus were obtained from nurseries in California and Oregon in Oct. 2000 and May 2001. (oregonstate.edu)

The report notes that the AAR, which bills itself as the leading citizen advocacy organization for improving the health of older Americans, "also happens to receive funding from private-sector biotechnology companies that have a financial stake in the outcome of the stem-cell debate, including Geron," the for-profit corporation that isolated embryonic stem cells in 1998. (christianitytoday.com)
Duane Gish, Ph.D. - The March 1998 Impact article"Cloning - What is It and Where is It Taking Us? (icr.org)
1998. Response of chaparral shrubs to below-freezing temperatures: acclimation, ecotypes, seedlings vs. adults. (oregonstate.edu)

Detailed descriptions of methods used in animal cloning and biotechnology are provided in the report Animal Biotechnology: Science-Based Concerns (NRC, 2002). (nationalacademies.org)
Today, December 27, 2002, it was announced that the first human clone was born at an undisclosed location. (probe.org)
December 27, 2002: First Human Clone Born? (hoaxes.org)
November 27, 2002: Imminent Birth of Human Clone? (hoaxes.org)
But in 2002 the Texans did produce Cc, the first cloned cat . (newscientist.com)
In January 2002, after a year of discussion and consultation, the group produced a report to CIHR's Governing Council, which was unanimously accepted and formed the basis of human pluripotent stem cell research guidelines that were publicly announced in March 2002. (gc.ca)
2002). In humans, SSEA4 is expressed by building the nervous system but also for their prospec- nonneural cells such as the erythrocytes (Kannagi et al. (lu.se)
explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)

Neurons that arise in the adult nervous system originate from neural stem cells and neural progenitor cells. (elifesciences.org)
Neural stem cells can also give rise to neural progenitor cells, which proliferate rapidly during their short lives and then 'differentiate' into neurons or glia. (elifesciences.org)
Consequently, neural stem and progenitor cells have usually been studied retrospectively, based on their ability to form colonies in laboratory cell cultures. (elifesciences.org)
A region of the brain called the subventricular zone contains both neural stem cells and neural progenitor cells, and is one of only two regions of the brain where neural stem cells are found in adult mammals. (elifesciences.org)
Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
In the context of hematopoietic cell transplantation, hematopoietic stem/progenitor cells (HSPC) from the umbilical cord blood (UCB) present several advantages compared to adult sources including higher proliferative capacity, abundant availability and ease of collection, non-risk and painless harvesting procedure, and lower risk of graft-versus-host disease. (bvsalud.org)
1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)

In response to the Donaldson report last August, the Government signalled their intention to make explicit what they thought was implicit in the Human Fertilisation and Embryology Act 1990 that human cloning for reproductive purposes was banned. (parliament.uk)
If we pass this Bill today, as I am sure we shall because everyone agrees with it our constituents are not likely to lobby us to vote against banning human reproductive cloning I shall still be disappointed because those more important issues will remain unregulated, although they are more relevant today in the light of recent developments. (parliament.uk)
One such thing is human reproductive cloning and, in particular, the implantation of a cell into a woman. (parliament.uk)
Since the term "born" has been used as an essential part of the definition of " reproductive cloning " used by Weissman, the National Academy of Sciences, etc., then it is critical to use the accurate term with the proper meaning. (lifeissues.net)
Reproductive cloning is expensive and highly inefficient. (wikiquote.org)

Keith Henry Stockman Campbell (23 May 1954 - 5 October 2012) was a British biologist who was a member of the team at Roslin Institute that in 1996 first cloned a mammal, a Finnish Dorset lamb named Dolly, from fully differentiated adult mammary cells. (wikipedia.org)

However, somatic cloning has been inefficient in all species in which live clones have been produced. (biomedcentral.com)
This also might have explained why cloning is inefficient: only 1-3% of cloned embryos eventually develop into an adult clone. (rupress.org)

Campbell had a key role in the creation of Dolly, as he had the crucial idea of co-ordinating the stages of the "cell cycle" of the donor somatic cells and the recipient eggs and using diploid quiescent or "G0" arrested somatic cells as nuclear donors. (wikipedia.org)
I was the Minister responsible for science at the time of the cloning of Dolly the sheep. (parliament.uk)
Member for Norwich, North (Dr. Gibson) mentioned the number of cells that had to fail so that Dolly could eventually be cloned. (parliament.uk)
Then in '97 the paper by Ian Wilmut came out on the cloning of Dolly the sheep. (rupress.org)
This question had not been resolved unequivocally by the cloning of Dolly or other mammals. (rupress.org)
Dolly the sheep had just been cloned. (newscientist.com)

Then, in 1996, a team led by Ian Wilmut with Keith Campbell as the main (66% of the credit) contributor used the same technique and shocked the world by successfully cloning a sheep from adult mammary cells. (wikipedia.org)

TEMRAs form aggregates with autologous platelets, release interferon gamma, and trigger platelet activation and apoptosis via the T-cell receptor-mediated release of cytotoxic granules. (medscape.com)
Apoptosis is a genetically controlled cell death that was initially recognized for its role in development. (jneurosci.org)
Nearly half of neural cells die by apoptosis during brain development. (jneurosci.org)
Eldadah and Faden, 2000 ), we hypothesized that suppression of apoptotic capability during maturation of mammalian brain results from repression of genes involved in the caspase-3 activation pathway, and that injury-induced neuronal apoptosis in the mature brain results from reactivation of these genes. (jneurosci.org)
After antigen recognition and activation, CD8 + cytotoxic T cells may trigger keratinocyte apoptosis. (medscape.com)
Shan J, Ma JM, Wang R, Liu QL, Fan Y. Proliferation and Apoptosis of Peripheral Blood Mononuclear Cells in Patients with Oral Lichen Planus. (medscape.com)
Current data suggest that oral lichen planus (OLP) is a T-cell-mediated autoimmune disease in which autocytotoxic CD8 + T cells trigger the apoptosis of oral epithelial cells. (medscape.com)

Somatic cell cloning (cloning or nuclear transfer) is a technique in which the nucleus (DNA) of a somatic cell is transferred into an enucleated metaphase-II oocyte for the generation of a new individual, genetically identical to the somatic cell donor (Figure 1 ). (biomedcentral.com)
As part of its charge, the committee was asked to prepare a subreport evaluating methods for detecting potential unintended compositional changes across the spectrum of messenger ribonucleic acid (mRNA), proteins, metabolites and nutrients that may occur in food derived from cloned animals that have not been genetically modified via genetic engineering methods. (nationalacademies.org)
In biology , cloning is the process of producing similar populations of genetically identical individuals that occurs in nature when organisms such as bacteria , insects or plants reproduce asexually . (wikiquote.org)
Developments in biotechnology have raised new concerns about animal welfare, as farm animals now have their genomes modified (genetically engineered) or copied (cloned) to propagate certain traits useful to agribusiness, such as meat yield or feed conversion. (wikiquote.org)

Bush promised in January to review a Clinton administration rule that allowed federal funding for researchers experimenting on embryo cells from fertility clinics. (christianitytoday.com)
The rule circumvented a 1995 congressional ban on using federal money for biomedical research on embryos outside the womb by allowing researchers to use stem cells extracted by a third party. (christianitytoday.com)
Researchers value the cells for their ability to replicate quickly and turn into any kind of human tissue. (christianitytoday.com)
The prolife lobby also received help from Do No Harm, a coalition of researchers, bioethicists, and doctors who spearheaded a nationwide petition urging Bush to oppose destructive human embryonic stem-cell research. (christianitytoday.com)
The groups argue that rather than waste embryos that will be destroyed along with their stem cells, researchers should use them to help save those whose lives are being cut short by disease. (christianitytoday.com)
These researchers demonstrated that adults with chronic ITP have clonal expansion of terminally differentiated effector memory CD8+ T cells (TEMRA), compared with age-matched controls. (medscape.com)
Two other independent researchers, Severino Antinori (an Italian working in an undisclosed Muslim country) and Panos Zavos (from Lexington, Kentucky) have also been hinting at human cloning success and suggesting that a birth will be announced soon. (probe.org)
This incredibly high 50% success rate for human cloning leaves most researchers believing that either this isn't really a clone or they simply aren't revealing all the other failures. (probe.org)
Texas A&M researchers announced that they had cloned a cat from the cumulus cell of an adult female cat. (hoaxes.org)
Oregon researchers announced the birth of Tetra, a rhesus macaque cloned by a process known as embryo splitting. (hoaxes.org)
Researchers at the University of Hawaii announce that mice have been successfully cloned from adult cells. (hoaxes.org)
Japanese researchers also announce the successful cloning of eight identical calves. (hoaxes.org)
The Korean researchers cloned the first dog in 2005, before you shut down. (newscientist.com)
Until then, Canada had no laws to govern human pluripotent stem cell research, nor were there any guidelines for researchers, research ethics boards, or funding agencies on how human pluripotent stem cells may be derived and used. (gc.ca)

The hemostatic system consists of platelets, coagulation factors, and the endothelial cells lining the blood vessels. (medscape.com)
These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)

In July 1995 Keith Campbell and Bill Ritchie succeeded in producing a pair of lambs, Megan and Morag from embryonic cells, which had differentiated in culture. (wikipedia.org)
1995) and subsequently in primary cells (Heyworth et al. (lu.se)

In 2000, after joining PPL Ltd, Campbell and his PPL team (based in North America) were successful in producing the world's first piglets by Somatic-cell nuclear transfer (SCNT), the so-called cloning technique. (wikipedia.org)
Because cattle are a species widely used for nuclear transfer studies, and more laboratories have succeeded in cloning cattle than any other specie, this review will be focused on somatic cell cloning of cattle. (biomedcentral.com)
The somatic cell and the oocyte is then fused (f) and the embryos is allowed to develop to a blastocyst in vitro (g). (biomedcentral.com)
discussed the procedure of cloning by somatic cell transfer. (icr.org)
체세포 핵 치환 (Somatic-cell nuclear transfer, SCNT)은 난자 의 핵 을 제거한 후에, 체세포 의 핵을 이식하여 복제 를 하는 기술을 말한다. (wikipedia.org)

Developmental defects, including abnormalities in cloned fetuses and placentas, in addition to high rates of pregnancy loss and neonatal death have been encountered by every research team studying somatic cloning. (biomedcentral.com)

The fact remains, however, that the Government's view last year was that the 1990 Act extended to embryos created by cell nuclear replacement. (parliament.uk)
Recommendation 2: In licensing any research using embryos created by cell nuclear replacement, the Human Fertilisation and Embryology Authority should satisfy itself that there are no other means of meeting the objectives of the research. (cmq.org.uk)

HN - 2008 BX - Von Ebner's Glands MH - Cumulus Cells UI - D054885 MN - A05.360.319.114.630.535.200.500 MN - A06.407.312.497.535.300.500 MN - A11.436.300.500 MS - The granulosa cells of the cumulus oophorus which surround the OVUM in the GRAAFIAN FOLLICLE. (bvsalud.org)
HN - 2008 BX - Granulosa Cells, Cumulus MH - Coronary Sinus UI - D054326 MN - A07.231.908.194.500 MS - A short vein that collects about two thirds of the venous blood from the MYOCARDIUM and drains into the RIGHT ATRIUM. (bvsalud.org)

In fact, low levels of telomerase activity have been found in human adult stem cells including haematopoietic and non-haematopoietic stem cells such as neuronal, skin, intestinal crypt, mammary epithelial, pancreas, adrenal cortex, kidney, and mesenchymal stem cells (MSCs) ( Table 1 ). (nature.com)
Bone marrow stromal cells (BMSCs, also known as bone marrow mesenchymal stem cells) are a plastic-adherent heterogeneous cell population that contain inherent skeletal progenitors and a subset of multipotential skeletal stem cells (SSCs). (bvsalud.org)
Here, we hypothesize that supplementation of embryo culture medium with extracellular vesicles from endometrial-derived mesenchymal stem cells (EV-endMSCs) may have a positive impact on the embryo competence of aged oocytes. (bvsalud.org)

2000. Diversification of the North American shrub genus Ceanothus (Rhamnaceae): conflicting phylogenies from nuclear ribosomal DNA and chloroplast DNA. (oregonstate.edu)
A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)

Unlike some other tissues, it has not been possible to identify or purify neural stem cells directly from the tissue. (elifesciences.org)
and altering cell and tissue characteristics for biomedical research and manufacturing. (nationalacademies.org)
In that procedure, the nucleus from a cell derived from an embryo, a fetus, or tissue of an adult is inserted. (icr.org)
Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
If there are intact cells in this tissue they have been 'stored' frozen. (wikiquote.org)
It is also our view that there are no sound reasons for treating the early-stage human embryo or cloned human embryo as anything special, or as having moral status greater than human somatic cells in tissue culture. (wikiquote.org)
A blastocyst (cloned or not), because it lacks any trace of a nervous system, has no capacity for suffering or conscious experience in any form - the special properties that, in our view, spell the difference between biological tissue and a human life worthy of respect and rights. (wikiquote.org)
and from mature adult tissue cells reprogrammed to behave like stem cells. (cmq.org.uk)
These three sources of stem cells do not create the same serious ethical concerns as those derived from embryonic and foetal tissue. (cmq.org.uk)
Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
The role of autoimmunity in the pathogenesis is supported by many autoimmune features of oral lichen planus, including its chronicity, onset in adults, predilection for females, association with other autoimmune diseases, occasional tissue-type associations, depressed immune suppressor activity in patients with oral lichen planus, and the presence of autocytotoxic T-cell clones in lichen planus lesions. (medscape.com)

They used adult cells, but it was possible that the cells that gave rise to successfully cloned animals were derived from rare adult stem cells. (rupress.org)

These developmental defects have been attributed to incomplete reprogramming of the somatic nuclei by the cloning process. (biomedcentral.com)
The vasopressin (VP) magnocellular neurosecretory cells (MNCs) in the supraoptic and paraventricular (PVN) nuclei are regulated by estrogen and exhibit robust expression of estrogen receptor (ER)-β. (jneurosci.org)
Systemic vasopressin (VP) and oxytocin (OT) are chiefly produced by magnocellular neurosecretory cells (MNCs) in the supraoptic (SON) and paraventricular (PVN) nuclei of the hypothalamus. (jneurosci.org)

Cloning by nuclear transfer using mammalian somatic cells has enormous potential application. (biomedcentral.com)
It was thought that mammalian cells might be refractory to cloning. (rupress.org)

Campbell's interest in cloning mammals was inspired by work done by Karl Illmensee and John Gurdon. (wikipedia.org)

Characterization of the human and mouse HEY1, HEY2, and HEYL genes: cloning, mapping, and mutation screening of a new bHLH gene family. (jax.org)
The basic helix-loop-helix genes Hesr1/Hey1 and Hesr2/Hey2 regulate maintenance of neural precursor cells in the brain. (jax.org)
It was also demonstrated that USF1 directly binds to the promoter region of 2,492 genes, which may be involved in the viral progression and cell proliferation pathways. (spandidos-publications.com)
Promoter region motif analysis indicated that ETV5 has more binding motifs and genes than USF1 itself for USF1‑regulated DEGs, indicating that USF1 may indirectly modulate gene expression by regulating ETV5 expression in Huh7 cells. (spandidos-publications.com)
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)

None of the few cells that were produced reached anywhere near blastocyst stage a mass of about 100 cells. (parliament.uk)
The blastocyst can then be transferred to a recipient (h) and cloned animals are born after completion of gestation (i). (biomedcentral.com)

Campbell believed all potential stem cell populations should be used for both basic and applied research which may provide basic scientific knowledge and lead to the development of cell therapies. (wikipedia.org)
Basically, given the difference of telomere and telomerase activity in human and mouse cells, the telomere and telomerase status in stem cell populations is different between humans and mice ( Harrington, 2004 ). (nature.com)
Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)

In somatic cells, the activity of telomerase, a reverse transcriptase that can elongate telomeric repeats, is usually diminished after birth so that the telomere length is gradually shortened with cell divisions, and triggers cellular senescence. (nature.com)
In most human somatic cells except for stem cells and lymphocytes, telomerase activity is diminished after birth so that telomere length shortens with each cell division. (nature.com)
A live birth of a primate clone will occur by January 1, 2000 and be reported within 6 months. (ideosphere.com)
If qualifying reports appear in at least three of the papers before July 1, 2000 (even if before January 1, 2000) that a live birth occurred before January 1, 2000, the claim will be judged true. (ideosphere.com)
In particular, as long as the report happens before July 1, 2000, and the birth happened before January 1, 2000, I will judge this claim YES. (ideosphere.com)
The Raelians have been hinting for months that a successful cloned birth was expected. (probe.org)
Even then, many of the clones which survive to birth develop complications in their first months of life, as high as 10% in cattle. (probe.org)
Reproduction and birth have always been subjects that quickly get people's attention, especially when you're talking about the extreme limits of the phenomena: multiple-births, clones, or the birth of creatures that seem to defy what some believe to be the natural order. (hoaxes.org)
Gathered here are examples of birth hoaxes throughout the ages, from the very recent claims about human cloning (which seem more and more likely to be a hoax) to the ancient. (hoaxes.org)
Dr. Brigitte Boisselier, chief executive of Clonaid, convened a press conference to announce the recent birth of 'Eve', a baby girl cloned from the skin cell of her 31-year-old mother. (hoaxes.org)
Dr. Severino Antinori, an Italian physician, announced that a woman who had participated in a scientific project that he assisted with would give birth to a human clone in January. (hoaxes.org)

He supported the use of SCNT for the production of personalised stem cell therapies and for the study of human diseases and the use of cybrid embryo production to overcome the lack of human eggs available for research. (wikipedia.org)
Again, Saunders is referring to SCNT as "THE" cloning procedure, when there are many other ways to clone a human being as well, and he is scientifically mis-defining the product of SCNT (i.e., the cloned human embryo). (lifeissues.net)

Transferring a terminally differentiated cell nucleus into an egg cell that has had its own nucleus removed wipes away the epigenetic marks of differentiation and allows the nucleus once again to code for any cell type. (rupress.org)
2004). In vivo and in vitro differentiation of male germ cells in the mouse. (edu.au)
The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)
However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)

Herein, we describe a scalable serum-free co-culture system for the expansion of UCB-derived CD34+-enriched cells using microcarrier-immobilized human bone marrow-derived mesenchymal stromal cells as feeder cells. (bvsalud.org)

Can Humans Be Cloned Like Sheep? (probe.org)

Nonetheless, the enhanced myogenesis leads to the formation of an extensive network of contractile myofibers, and this enhanced myogenesis can be conferred upon myogenic cells from clonal populations, such as G8 or C2C12 cells, if they are co-cultured with the limb mesenchymal cells. (biologists.com)

Sugerman PB, Satterwhite K, Bigby M. Autocytotoxic T-cell clones in lichen planus. (medscape.com)

In nature, things get cloned and we mix genetics each time that we crossbreed animals. (parliament.uk)
He is the co-author of the book The Natural Limits to Biological Change , served as general editor of Creation, Evolution and Modern Science , co-author of Basic Questions on Genetics, Stem Cell Research and Cloning (The BioBasics Series) , and has published numerous journal articles. (probe.org)

I asked whether a terminally differentiated cell is still amenable to reprogramming and able to give rise to a cloned animal. (rupress.org)
In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)

Currently, the efficiency for nuclear transfer is between 0-10%, i.e., 0-10 live births after transfer of 100 cloned embryos. (biomedcentral.com)
Various strategies have been employed to modify donor cells and the nuclear transfer procedure in attempts to improve the efficiency of nuclear transfer. (biomedcentral.com)
Although the efficiency of nuclear transfer has been dramatically improved from the initial success rate of one live clone born from 277 embryo transfers [ 1 ], none of the aforementioned efforts abolished the common problems associated with nuclear transfer. (biomedcentral.com)
The advent of techniques to propagate animals by nuclear transfer, also known as cloning, potentially offers many important applications to animal agriculture, including reproducing highly desired elite sires and dams. (nationalacademies.org)
Nuclear transfer is one of the most sophisticated, fickle, and challenging techniques in cell biology. (rupress.org)
More than 100 nuclear transfer procedures could be required to produce one viable clone. (wikiquote.org)

Conditional deletion of the Bmi-1 polycomb protein depleted pre-GEPCOT and GEPCOT cells, though pre-GEPCOT cells were more dependent upon Bmi-1 for Cdkn2a ( p16 Ink4a ) repression. (elifesciences.org)
He had also developed a method to clone predynorphin, the precursor protein which forms first in the genetic sequence that expresses dynorphin. (nih.gov)
These glycoproteins include the CD98 heavy chain protein of Mus musculus (gbU25708) and the orthologous 4F2 cell surface antigen heavy chain of Homo sapiens (spP08195). (tcdb.org)
Full length human recombinant protein of human TP63 (NP_003713) produced in SF9 cell. (origene.com)
Besides, the protein formation and other functions in cells can be several orders of magnitude more information-intensive. (oversigma.com)
In healthy individuals, nicotine improves memory impairment caused by sleep deprivation by enhancing the phosphorylation of calmodulin‑dependent protein kinase II, an essential regulator of cell proliferation and synaptic plasticity. (spandidos-publications.com)

The Raelians believe that the successful cloning of a human being will be the first step on the path towards immortality. (hoaxes.org)
It seems possible that Dr. Antinori's announcement might have prompted the Raelians to quickly get in on the act and establish their priority in the race to be the first to clone a human. (hoaxes.org)

While research on human adult stem cells was not included in the Working Group's mandate, recent scientific research has confirmed the possibility of generating human pluripotent stem cells with properties similar to embryonic stem cells from adult cells (e.g., induced pluripotent stem cells). (gc.ca)

Hochedlinger pushes cells' developmental rewind buttons to examine their epigenetic history. (rupress.org)

Biotechnology companies specializing in stem-cell research stand to reap huge financial windfalls from successful therapies developed via this science," said the CPI report. (christianitytoday.com)
Cloning in biotechnology refers to processes used to create copies of DNA fragments ( molecular cloning ), cells (cell cloning), or organisms . (wikiquote.org)

Five of the clones spontaneously aborted within three weeks while the other five have continued without complication. (probe.org)

Cells are collected from donor (a) and cultured in vitro (b). (biomedcentral.com)
A matured oocyte (c) is then enucleated (d) and a donor cell is transferred into the enucleated oocyte (e). (biomedcentral.com)
Most of these efforts are focused on donor cells. (biomedcentral.com)
We had the dubious distinction of having produced the world's first clone that did not resemble its genetic donor . (newscientist.com)
Cell kill indicates that the recipient has antibodies against the donor cells. (medscape.com)

Since neurosphere-forming cells can self-renew and differentiate into neurons and glia, the ability of cells to form neurospheres has generally been taken as evidence that they are stem cells. (elifesciences.org)
However, the exact relationship between neural stem cells and neurosphere-forming cells has been uncertain. (elifesciences.org)
have used a technique called flow cytometry to identify and isolate neural stem cells and neurosphere-forming cells directly from the subventricular zone. (elifesciences.org)
The neurosphere-forming cells, named GEPCOT cells, were short-lived and highly proliferative in the brain. (elifesciences.org)

2000. The effect of raloxifene on the uterine weight response in immature mice exposed to 17- estradiol, 1,1,1-trichloro-2,2- bis ( p -chlorophenyl)ethane, and methoxychlor. (cdc.gov)
A) Sex differences in density of -gal+ (PR+) cells in adult PRPL/PL mice. (cdc.gov)
F) The vast majority of -gal+ (PR+) cells in various regions in PRPL mice are neurons, as evidenced by co-labeling with NeuroTrace (fluorescent Nissl) in the arcuate and NeuN for the remaining regions. (cdc.gov)
G) No sex difference in nuclear or soma size of PR+ VMHvl cells in PRPL mice. (cdc.gov)
E, F) Cre expression mirrors that of PR in the VMHvl of adult PRCre/+ mice in adjacent sections. (cdc.gov)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)

The reason I then was drawn into epigenetics and stem cell biology was a lecture at the IMP by Rudolf Jaenisch. (rupress.org)

Neurosphere formation is commonly used as a surrogate for neural stem cell (NSC) function but the relationship between neurosphere-initiating cells (NICs) and NSCs remains unclear. (elifesciences.org)
Eve was delivered by Caesarian section from her twin sister (the woman who donated the nuclear genetic material from which she was cloned also served as the surrogate mother). (probe.org)

The dense sub-epithelial mononuclear infiltrate in oral lichen planus is composed of T cells and macrophages, and there are increased numbers of intra-epithelial T cells. (medscape.com)
Therefore, early in the formation of oral lichen planus lesions, CD8 + T cells may recognize an antigen associated with the major histocompatibility complex (MHC) class I on keratinocytes. (medscape.com)
[ 8 ] T cells in oral lichen planus contain mRNA for TNF and secrete TNF in vitro. (medscape.com)
Oral lichen planus affects approximately 1-2% of the general adult population, although the prevalence of the disease is unknown in many areas. (medscape.com)
[ 22 ] Oral lichen planus is a common noninfectious oral mucosal disorder among adult patients who attend oral pathology and oral medicine clinics. (medscape.com)
Oral lichen planus predominantly occurs in adults older than 40 years, although younger adults and children can be affected. (medscape.com)
Reported associations between oral lichen planus and systemic diseases may be coincidental, because (1) oral lichen planus is relatively common, (2) oral lichen planus occurs predominantly in older adults, and (3) many drugs used in the treatment of systemic diseases trigger the development of oral lichenoid lesions as an adverse effect. (medscape.com)

7. "[footnote 16]: The cloning procedure supplies the oocyte with a complete set of chromosomes, all of which are contained in the nucleus which is transferred into the denucleated oocyte. (lifeissues.net)

A critical length of telomere repeats is required to ensure proper telomere function and avoid the activation of DNA damage pathways that result in replicative senescence or cell death. (nature.com)
Therefore, it's necessary to express Cs sPLA2 in water-soluble form and determine whether the enzymatic activity of Cs sPLA2 or cell signalling pathways is involved in liver fibrosis caused by clonorchiasis. (biomedcentral.com)

however, the level of telomerase activity is low or absent in the majority of stem cells regardless of their proliferative capacity. (nature.com)
As stem cells have elongated proliferative capacity, they should have a mechanism that maintains telomere length through many cell divisions. (nature.com)

We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)

Today, this unconventional biotech entrepreneur shares his home with three Missy clones - Mira and her younger sisters, Chingu and Sarang - thanks to a link-up with South Korea's disgraced cloning expert Woo Suk Hwang . (newscientist.com)
Taking creatine by mouth seems to somewhat improve muscle strength in both younger and older adults. (medlineplus.gov)

ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)

When JNK signalling pathway is blocked by inhibitor SP600125, hepatic stellate cells (HSCs) activation is inhibited, with less proliferation and reduced expression of α-smooth muscle actin (α-SMA) [ 15 ]. (biomedcentral.com)

Anatomy19

Organisms4

Chemicals and Drugs22

Analytical, Diagnostic and Therapeutic Techniques and Equipment21

Phenomena and Processes45

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Named Groups1

Health Care2

Therapeutic9

19975

20013

19983

20028

Progenitor cells9

Reproductive5

Differentiated adult mammary1

Inefficient2

Dolly6

19961

Apoptosis7

Genetically4

Researchers14

Endothelial cells4

19952

Somatic cell5

Fetuses1

Embryos created by cell nuclear replacement2

Cumulus2

Mesenchymal stem3

Diversification2

Tissue12

Successfully1

Nuclei3

Mammalian2

Mammals1

Genes6

Blastocyst2

Stem cell populations3

Birth11

SCNT2

Differentiation12

Bone marrow1

Sheep1

Populations1

Autocytotoxic1

Genetics2

Terminally differentiated cell2

Nuclear transfer6

Protein6

Raelians2

Human adult stem1

Developmental1

Biotechnology2

Spontaneously1

Donor5

Neurosphere-formi4

Mice6

Biology1

Surrogate2

Oral Lichen7

Procedure1

Pathways2

Proliferative capacity2

Hematopoietic1

Younger2

Abstract1

Proliferation1