• Neonatal rat cardiomyocytes were isolated and subjected to hypoxia/reoxygenation (H/R) injury, with or without HPC. (spandidos-publications.com)
  • HPC protected neonatal rat cardiomyocytes against H/R injury by increasing cell viability, while reducing LDH release and cell apoptosis. (spandidos-publications.com)
  • KCNQ channels are involved in the regulatory volume decrease response in primary neonatal rat cardiomyocytes. (ku.dk)
  • In addition, neonatal rat cardiomyocytes (NRCMs), cardiac fibroblasts (CFs), and macrophages were isolated for in vitro experiments. (hindawi.com)
  • We established a novel technique for quantifying [Na + ] i in cultured neonatal rat cardiomyocytes attached to a 96-well plate using a microplate reader in combination with SBFI and probenecid. (biomedcentral.com)
  • Research led by Dr Alzbeta Hulikova and Professor Pawel Swietach has, for the first time, described the potential regulation of nuclear acid-base chemistry in neonatal and adult cardiomyocytes, and explained its relevance in the context of heart physiology and pathology. (ox.ac.uk)
  • By growing cardiac myocytes from neonatal rats in media with different pH, the team were able to identify genes which are pH-sensitive. (ox.ac.uk)
  • For the first time, researchers have described the possible regulation of nuclear acid-base chemistry in neonatal and adult cardiomyocytes. (ox.ac.uk)
  • Using immunoblotting, we established that moderate levels of H2O2 activate ASK1 in neonatal rat cardiomyocytes and perfused rat hearts. (ox.ac.uk)
  • Tahrir FG, Gordon J, Feldman AM, Cheung J, Khalili K, Mohseni Ahooyi T ( 2019 ) Evidence for the impact of BAG3 on electrophysiological activity of primary culture of neonatal cardiomyocytes. (multichannelsystems.com)
  • Vesicular trafficking genes affected by AS during normal development (when Celf1 is down-regulated) show a reversion to neonatal splicing patterns after Celf1 re-expression in adults. (abt-888.net)
  • Cardiomyocyte hypertrophy is a mechanism by which myocardial mass is increased to compensate for any elevated physical demands placed upon the heart, thus ensuring that adequate perfusion of body tissues is maintained during these periods. (europeanpharmaceuticalreview.com)
  • These cells can be treated with different agents to stimulate cardiomyocyte hypertrophy in vitro or induce collagen synthesis in cardiac fibroblasts. (gcure.nl)
  • Systemic hypertension increases cardiac workload causing cardiomyocyte hypertrophy and increased cardiac fibrosis. (ox.ac.uk)
  • However, it suppressed hypertension-induced cardiac hypertrophy resulting from angiotensin II (0.8 mg/[kg·d], 7d), with inhibition of Nppa/Nppb mRNA upregulation, reduced cardiomyocyte hypertrophy and, notably, significant reductions in interstitial and perivascular fibrosis. (ox.ac.uk)
  • However, the knockdown of miR‑133b‑5p in the cardiomyocytes blocked HPC‑mediated cardioprotection as reflected by the aggravation of cell injury and apoptosis. (spandidos-publications.com)
  • The aim of the present study was to investigate the effect of grape seed extract consumption with moderate intensity aerobic exercise on some markers of cardiac myocyte apoptosis in male Wistar rats with type 1 diabetic. (magiran.com)
  • Losmapimod effectively improved memory, reduced the frequency of seizures, protected the neuron from damage, and limited the apoptosis of neurons in epilepsy rats. (frontiersin.org)
  • We found that Fgl2 gene silencing inhibits apoptosis and improves heart function of streptozotocin (STZ)-induced diabetes rats, the possible mechanism maybe that Fgl2 gene silencing reduces the tumour necrosis factor (TNF)±levels, decreases the expression of B-cell lymphoma-2 (bcl2), bcl-2-associated X (bax), toll-like receptors 4 (TLR4) and p38 mitogen-activated protein kinase (MAPK). (silverchair.com)
  • The aim of this study was to investigate whether resveratrol (RSV) could ameliorate ischemia- and hypoxia-associated cardiomyocyte apoptosis and injury via inhibiting senescence signaling and inflammasome activation. (hindawi.com)
  • Hypoxia/reoxygenation (H/R)-induced cardiomyocyte cell apoptosis is critical in developing myocardial infarction. (biomedcentral.com)
  • Our data speculated that STA protects H/R injury and inhibits oxidative stress and apoptosis in cardiomyocytes by activation of the SIRT1-Nrf2 pathway. (biomedcentral.com)
  • Adult rats were subjected to myocardial ischemia/reperfusion (I/R) injury with or without ischemic preconditioning (IPC), and the level of miR‑133b‑5p in myocardium was measured. (spandidos-publications.com)
  • Cardioprotective Effect of Ranolazine in the Process of Ischemia-reperfusion in Adult Rat Cardiomyocytes [J].2016,(1). (wanfangdata.com.cn)
  • ASK1 was activated during ischemia in adult rat hearts, but not on reperfusion, consistent with activation by moderate (not high) reactive oxygen species levels. (ox.ac.uk)
  • During ischemia and reoxygenation of the myocardium, the levels of free radicals increase in cardiomyocytes and Ca2+ transport as well as contractility are impaired due to oxidative damage to the ion-transporting protein complexes. (cardioweb.ru)
  • We study the mechanisms of protection of cardiomyocytes under ischemia and oxidative stress. (cardioweb.ru)
  • Our previously-obtained impressive results of highly increased C2C12 mouse myoblast adhesion to amine plasma polymers (PPs) motivated current detailed studies of cell resistance to trypsinization, cell proliferation, motility, and the rate of attachment carried out for fibroblasts (LF), keratinocytes (HaCaT), rat vascular smooth muscle cells (VSMC), and endothelial cells (HUVEC, HSVEC, and CPAE) on three different amine PPs. (nature.com)
  • We can isolate and culture cardiomyocytes and cardiac fibroblasts (see Table 2). (gcure.nl)
  • Human and rat hearts are composed of 66% cardiac fibroblasts (CF) 30 cardiomyocytes (CM) and 4% endothelial and vascular smooth muscle cells5-7. (abt-888.net)
  • Methodology Adult Rat Ventricular Myocytes (ARVMs) were isolated from male Wistar rats by enzymatic digestion and placed into primary culture. (bmj.com)
  • Forty adult male Wistar rats with an initial weight range of 160-220 g were divided into 5 groups of including diabetic / training + extract, diabetic / training, diabetic / extract, diabetic / control and healthy / control (8 heads per group). (magiran.com)
  • Cell labeling, tracking, and molecular biology techniques indicated that the grafted cardiomyocytes survived in the rat heart 1 month after iPSC-CM transplantation. (biomedcentral.com)
  • Damaged or stressed cells, cardiomyocytes, in particular, emit damage-associated molecular patterns (DAMPs) including HMGB1, S100 A8/A9, HSP70, and other molecules, unfolding paracrine mechanisms that induce an innate immune response. (highwire.org)
  • Myocardial infarction (MI), the most common heart disease, is characterized by a significant reduction in the number of functional cardiomyocytes and leads to the development of progressive heart failure. (biomedcentral.com)
  • Treatment with the gap junction modifier rotigaptide (ZP123) reduces infarct size in rats with chronic myocardial infarction. (ku.dk)
  • Eight-week-old male Sprague-Dawley rats were randomly selected to receive an intramyocardial injection of 5% albumin solution with or without 1 × 10 7 human iPSC-CMs 10 days after undergoing left anterior descending (LAD) coronary artery ligation. (biomedcentral.com)
  • We were interested in the developmental potential of single cardiac progenitor cell into cardiomyocytes, smooth muscle cells, and endothelial cells. (stanford.edu)
  • Qian Y, Qian F, Zhang W, Zhao L, Shen M, Ding C, Guo J ( 2019 ) Shengjiang Powder ameliorates myocardial injury in septic rats by downregulating the phosphorylation of P38-MAPK. (multichannelsystems.com)
  • Efficacious and safe intramyocardial delivery of modified mRNA encoding cell cycle regulators as a gene therapy for cardiac regeneration through resident cardiomyocyte proliferation. (ca.gov)
  • We propose gene delivery mediated by modified mRNA, as used in COVID vaccines, to reprogram adult cardiomyocytes transiently into a proliferative state for cardiac regeneration, thereby improving heart function. (ca.gov)
  • Transplantation of embryonic cardiomyocytes after extensive MI in a rat model attenuate LV dilatation, infarct thinning, and myocardial dysfunction. (nih.gov)
  • Development provides an outstanding opportunity to identify coordinated AS regulation critical for physiological transitions from embryonic to adult functions. (abt-888.net)
  • Previously we showed that genes that undergo AS regulation during heart development produce transitions from GSK1324726A embryonic to adult protein isoforms GSK1324726A largely without changes in overall transcript levels presenting a new paradigm for understanding developmentally regulated gene expression in heart3. (abt-888.net)
  • Based on their origin, there are two main types of stem cells - embryonic stem cells (ES cells) and postnatal or adult stem cells (AS cells). (bvsalud.org)
  • Previous studies have shown that the stimulation of adenosine type 1 receptors (A1.Rs) induces PP2AC carboxylmethylation and altered subcellular distribution in adult rat ventricular myocytes (ARVM). (kingston.ac.uk)
  • A well-established method of cardiomyocyte differentiation from PSCs provides an ideal source of cardiomyocytes and makes regenerative therapy for myocardial muscle damage a promising strategy. (biomedcentral.com)
  • We applied this method to computationally combine confocal-scale (~ 200 nm) data of RyR clusters with 3D electron microscopy data (~ 30 nm) of myofibrils and mitochondria, both collected from adult rat left ventricular myocytes. (pubchase.com)
  • We report that both the intramyocellular circadian clock and diurnal variations in fatty acid responsiveness observed in the intact rat heart in vivo persist in adult rat cardiomyocytes. (elsevierpure.com)
  • Chronic overexpression of MT or acute in vitro treatment with the flavoprotein inhibitor diphenyleneiodonium or the angiotensin II type I receptor antagonist losartan eliminated excess ROS production in diabetic cardiomyocytes. (diabetesjournals.org)
  • Efficacy of modRNA delivery of cell cycle regulators on improving cardiac function in chronic post-MI rats. (ca.gov)
  • To illustrate the utility of the new method, we also examined the chronic effects of aldosterone on [Na + ] i in cultured cardiomyocytes. (biomedcentral.com)
  • N -acetylcysteine prevents the deleterious effect of tumor necrosis factor-alpha on calcium transients and contraction in adult rat cardiomyocytes. (edu.pl)
  • To investigate this hypothesis, the H9c2 cardiomyocyte cell line was used to establish an in vitro myocardial H/R injury model to explore the roles and potential mechanisms of STA in myocardial H/R injury. (biomedcentral.com)
  • To address these hypotheses, we examined contractility, Ca 2+ handling, and ROS levels in individual cardiomyocytes isolated from control hearts, diabetic OVE26 hearts, and diabetic hearts overexpressing antioxidant protein metallothionein (MT). Our data showed that diabetic myocytes exhibited significantly reduced peak shortening, prolonged duration of shortening/relengthening, and decreased maximal velocities of shortening/relengthening as well as slowed intracellular Ca 2+ decay compared with control myocytes. (diabetesjournals.org)
  • Modulation of severity of reperfusion stunning in the isolated rat heart by agents altering calcium flux at onset of reperfusion. (wanfangdata.com.cn)
  • Short-term Celf1 induction in adult animals results in disrupted transverse tubule organization and calcium handling. (abt-888.net)
  • Upregulation of miR‑133b‑5p contributes to HPC‑mediated cardioprotection in cardiomyocytes, and the mechanism may be associated with inhibition of caspase‑8 and caspase‑3 apoptotic signaling. (spandidos-publications.com)
  • Amend N, Thiermann H, Worek F, Wille T ( 2019 ) The arrhythmogenic potential of nerve agents and a cardiac safety profile of antidotes - A proof-of-concept study using human induced pluripotent stem cells derived cardiomyocytes (hiPSC-CM). Toxicol Lett 308:1-6. (multichannelsystems.com)
  • Kraushaar U, Guenther E ( 2019 ) Assay Procedures for Compound Testing of hiPSC-Derived Cardiomyocytes Using Multiwell Microelectrode Arrays. (multichannelsystems.com)
  • The first four postnatal weeks involve a period of extensive physiological remodeling with dynamic changes as the fetal heart adapts to birth and converts to adult function. (abt-888.net)
  • We believe that by understanding the mechanisms regulating the formation of the heart during fetal development we can then apply these principles to understand the causes of adult heart diseases such as heart attack and heart failure. (stanford.edu)
  • Human iPSC-CM grafts survived in infarcted rat hearts and restored myocardial function 4 weeks after transplantation. (biomedcentral.com)
  • Heart regenerative therapy, such as transplantation of myocytes [ 4 ] and cardiomyocytes to replace lost cells, has become a new strategy for the treatment of heart failure caused by MI [ 5 ]. (biomedcentral.com)
  • Seven days after extensive MI, rats were randomized to cell (1.5x10(6)) transplantation (n=11) or culture medium injection (n=16) into the myocardial scar. (nih.gov)
  • CONCLUSIONS: Adults with IBD were more likely to receive many types of preventive care than adults without IBD. (cdc.gov)
  • Human-induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) have shed great light on cardiac regenerative medicine and specifically myocardial repair in heart failure patients. (biomedcentral.com)
  • Literature review: This review summarizes current knowledge, barriers, and challenges in the clinical use of adult stem cells, scaffolds, and growth factors for the development and evaluation of regenerative endodontic therapies. (bvsalud.org)
  • The most valuable cells for regenerative endodontics are postnatal or adult stem cells. (bvsalud.org)
  • This cardiomyopathy may be due to either organ-based damage, such as fibrosis, or to direct damage to cardiomyocytes. (diabetesjournals.org)
  • ANP, BNP), Western blot protein analysis, in particular post-translational modifications (e.g. phospho-Akt), histochemistry to determine cardiomyocyte size, capillary density and fibrosis, and immuno-histochemistry. (gcure.nl)
  • In addition, high glucose and angiotensin II promoted significantly increased generation of ROS in diabetic cardiomyocytes. (diabetesjournals.org)
  • By combining the antioxidant and anti-inflammatory effects of moderate-intensity aerobic exercise along with the consumption of grape seed extract, more anti-apoptotic effects may be induced in the myocytes of type 1 diabetic rats. (magiran.com)
  • Activation of the MAPK signaling pathway was observed in epilepsy rats. (frontiersin.org)
  • ASK1 was not activated by IL1β in cardiomyocytes and activation in perfused hearts was due to increased reactive oxygen species. (ox.ac.uk)
  • During postnatal development the heart undergoes a rapid and dramatic transition to adult function through transcriptional and post-transcriptional mechanisms including alternative splicing (AS). (abt-888.net)
  • Specifically, the endogenous H 2 S level in the substantia nigra (SN) is significantly reduced along with 6-hydroxydopamine (6-OHDA) treatment in rats, while supplementation of H 2 S not only reverses 6-OHDA-induced neuronal loss but also attenuates the following disorders of movement, suggesting a protective effect of H 2 S in Parkinson's disease (PD). (springer.com)
  • Therefore, the purpose of this study was to investigate the involvement of cholinergic neurotransmission present within PL and IL in cardiovascular responses to an acute session of restraint stress in rats. (bvsalud.org)
  • BACKGROUND: Previous reports suggest that adults with inflammatory bowel disease (IBD) receive suboptimal preventive care. (cdc.gov)
  • This in turn leads to a reduction in the exchange of respiratory gasses and nutrients between working cardiomyocytes and blood which results in reduced energy levels in these cells. (europeanpharmaceuticalreview.com)
  • Heart muscle cells die en masse after injury, yet the adult mammalian heart retains little capacity to regenerate them. (natureasia.com)
  • The survival of grafted cardiomyocytes was confirmed by observing the fluorescent cell tracer Vybrant™ CM-DiI or expression of the enhanced green fluorescent protein (eGFP) in transplanted cells, or survival was demonstrated by polymerase chain reaction (PCR)-based detection of human mitochondrial DNA. (biomedcentral.com)
  • Rat cardiomyocyte H9c2 cells underwent H/R (hypoxia for 4 h and reoxygenation for 12 h). (biomedcentral.com)
  • Cells were pretreated with STA (50 µM) 2 h before H/R. Cardiomyocyte injury was evaluated by CCK-8 assay and lactate dehydrogenase (LDH) release. (biomedcentral.com)
  • Selonsertib (ASK1 inhibitor) prevented activation of p38-MAPKs (but not JNKs) by oxidative stresses in cultured cardiomyocytes and perfused hearts. (ox.ac.uk)
  • Cardiac myosin binding protein-C (cMyBP-C) is one of the proteins that make up the contractile apparatus of cardiomyocytes. (scirp.org)
  • Longman, Michael R. , Ranieri, Antonella , Avkiran, Metin and Snabaitis, Andrew K. (2014) Regulation of PP2AC carboxylmethylation and cellular localisation by inhibitory Class G-Protein coupled receptors in cardiomyocytes. (kingston.ac.uk)
  • Energy starvation in hypertrophied cardiomyocytes is believed to occur as a result of a combination of factors, such as the reduction in the ratio in the surface area between capillaries and cardiomyocytes and increased myocyte diameter, see Figure 1 3 . (europeanpharmaceuticalreview.com)
  • The Mouse Adult Gross Anatomy Ontology and Mammalian Phenotype Ontology are downloaded weekly from the Mouse Genome Informatics databases at Jackson Laboratories (ftp://ftp.informatics.jax.org/pub/reports/index.html). (mcw.edu)
  • Still, many grafts remain isolated and do not differentiate into an adult phenotype, even when studied 2 months after grafting. (nih.gov)
  • NF- κ B and AP-1 are chronically activated in cardiomyocytes in human heart failure. (vin.com)
  • Human iPS-derived cardiomyocytes successfully transduced with modified RNA (modRNA) encoding human cell cycle regulators. (ca.gov)
  • The objective of this study was to evaluate the ability of human iPSC-CMs to improve myocardial function in a rat postinfarction heart failure model. (biomedcentral.com)
  • Finally, using our unique method for generating RyR cluster distributions, we demonstrate the robustness in the [Ca2+]i transient to differences in RyR cluster distributions measured between rat and human cardiomyocytes. (pubchase.com)
  • Adults with diabetes are 2-4 times more likely to die of heart failure than the rest of the population. (silverchair.com)
  • These nuclear pH differences are important in heart pathology, and are significantly dysregulated in myocytes from cryo-infarcted adult rats or sheep with heart failure. (ox.ac.uk)
  • Vascular endothelial cell (EC)-derived factors play an important role in endothelial-cardiomyocyte crosstalk and could save cardiomyocytes (CMs) from injury. (mdpi.com)
  • Therefore, the study aimed to investigate the protective effects and mechanisms of STA on H/R injury of cardiomyocytes. (biomedcentral.com)
  • However, the role of STA on the H/R injury of cardiomyocytes through the SIRT1-Nrf2 pathway remains unexplored. (biomedcentral.com)
  • RESULTS: The prevalence of a doctor visit in the past 12 months was significantly higher among adults with IBD than those without. (cdc.gov)
  • 2010 ). In addition, the p38 MAPK inhibitor effectively shortened the duration of seizures in epileptic rats (Yang et al. (frontiersin.org)
  • For this, rats received bilateral microinjection of the choline uptake inhibitor hemicholinium-3 before exposure to restraint stress. (bvsalud.org)
  • Evaluate safety parameters for modRNA delivery of cell cycle regulators in mice and rats. (ca.gov)