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AdsorptionChromatography, High Pressure LiquidChromatography, AffinityChromatography, GelGelsChromatographyChromatography, Ion ExchangeChromatography, GasChromatography, Thin LayerChromatography, LiquidMolecular WeightChromatography, DEAE-CelluloseElectrophoresis, Polyacrylamide GelKineticsChromatography, AgaroseMolecular Sequence DataAmino Acid SequenceMass SpectrometryHydrogen-Ion ConcentrationGas Chromatography-Mass SpectrometrySurface PropertiesChromatography, Reverse-PhaseSepharoseAmino AcidsChromatography, PaperHydroxyapatitesSubstrate SpecificityCattleElectrophoresis, Gel, Two-DimensionalTemperatureSilicon DioxideEscherichia coliChemistryIsoelectric FocusingChemical PhenomenaSpectrophotometry, UltravioletMethodsTandem Mass SpectrometryProtein BindingRabbitsChemical FractionationCarbohydratesTrypsinDrug StabilitySpectrometry, Mass, Electrospray IonizationPeptidesHydrolysisPeptide FragmentsSolubilityBase SequenceLiverSurface-Active AgentsIsoelectric PointCountercurrent DistributionMagnetic Resonance SpectroscopyRecombinant ProteinsBinding SitesCarbohydrate SequenceChromatography, Micellar Electrokinetic CapillaryDurapatiteIndicators and ReagentsBentoniteChemical PrecipitationProteinsOligosaccharidesImmunodiffusionElectrophoresis, Agar GelElectrophoresisMacromolecular SubstancesPolystyrenesCell LineMicrochemistryHot TemperatureBacterial ProteinsSurface TensionCharcoalPolysaccharidesPhospholipidsRadioimmunoassayCloning, MolecularElectrophoresis, Gel, Pulsed-FieldWaterAnion Exchange ResinsSpecies SpecificityGlycoproteinsSerum Albumin, BovineChemistry, PhysicalPhysicochemical PhenomenaCalibrationAmmonium SulfateImmune SeraCentrifugation, Density GradientSwinePolymersCelluloseMolecular StructureElectrochemistryBlood ProteinsLectinsHydrophobic and Hydrophilic Interactions
Normal-phase liquid chromatographySilica gelHPLCThin-layer chromatoDesorptionAffinity chromatographyPermeation ChromatographyHydrophobic chromatographySelectivityFiltrationSolventAnalyticalStationary phaseSubstrateCompoundsSubstancesMoleculesSolventsMolecularly imprintedExtractionEluentChemistryEfficiencyMethodChromatographicExchangeColumnSalt concentrationSurfacesHydrophobicityLiquidProcessWidelyMethodsSolidConditionsSurfaceSampleTimeMagneticArticleChemicalSeparateMedium
Normal-phase liquid chromatography3
Silica gel8
HPLC8
  • Finally, the optimized DMIPs-MMMs method coupled with HPLC-MS/MS was validated and implemented to extract and detect five ATSs from wastewater and urine samples. (chromatographyonline.com)
  • The wide variety of equipment, columns, eluent and operational parameters involved makes high performance liquid chromatography (HPLC) method development seem complex. (pharmtech.com)
  • A theoretical description of analyte retention in high-performance liquid chromatography (HPLC) has been the subject of various publications. (springer.com)
  • Hydrophilic interaction liquid chromatography (HILIC) is an alternative high-performance liquid chromatography (HPLC) mode for separating polar compounds. (springer.com)
  • In the present study, cordycepin was prepared by preparative high‑performance liquid chromatography (prep-HPLC) and the purity obtained was 99.6%, indicating that this technique may be useful for the large‑scale isolation of cordycepin in the future. (spandidos-publications.com)
  • HPLC, high-performance liquid chromatography. (spandidos-publications.com)
  • Preparative high performance liquid chromatography (prep-HPLC) can shorten the production cycle, has relatively low operating costs, and is simple to carry out. (spandidos-publications.com)
  • In this paper, a new rapid and sensitive method based on sodium dodecyl sulfate modified Fe3O4@α-Linolenic acid nanocomposite combined with high-performance liquid chromatography-photo diode array detection (HPLC-PDA) has been proposed for the extraction and determination of tramadol (TRA) in water samples. (ac.ir)
Thin-layer chromato4
Desorption3
  • The adsorption and desorption conditions for MMIPs-SERS detection system were carefully investigated. (bvsalud.org)
  • The purpose of the flash column, that is, without sacrificing exchange efficiency (adsorption and desorption), use the fastest possible flow rate to reduce this lateral diffusion. (flashcolumns.com)
  • The main factors influencing extraction and desorption efficiency were optimized. (ac.ir)
Affinity chromatography3
Permeation Chromatography1
  • In this sense, the physicochemical characterisation of different industrial (technical) lignins are performed exploiting different methods, like Brunauer-Emmett-Teller N2 adsorption, particle size distribution, Fourier transform infrared spectroscopy, ultraviolet-visible absorption spectroscopy, gel permeation chromatography, and thermogravimetric analysis. (lu.se)
Hydrophobic chromatography3
  • Since the hydrophobic characteristics of the target product are difficult to obtain directly, when designing a hydrophobic chromatography process, pre-experiments should be carried out with fillers containing different hydrophobic ligands. (biolink.com)
  • Hydrophobic chromatography is a chromatographic mode for purification based on the difference in hydrophobicity between the target product and impurities. (biolink.com)
  • Generally speaking, hydrophobic chromatography is mainly used for the removal of high molecular weight polymers. (biolink.com)
Selectivity2
  • The adsorption capacity and selectivity of DMIPs were also investigated. (chromatographyonline.com)
  • Mobile phase composition, for example, is the most powerful way of optimizing selectivity whereas temperature has a minor effect and would only achieve small selectivity changes. (pharmtech.com)
Filtration1
  • Because the separation principle of hydrophobic interaction chromatography is completely different from chromatographic techniques such as ion exchange chromatography or gel filtration chromatography, this technique is often used in combination with the latter two to separate complex biological samples. (welch-us.com)
Solvent7
  • Column chromatography can be done using gravity to move the solvent, or using compressed gas to push the solvent through the column. (wikipedia.org)
  • Because we use compressed air to push the solvent through the column, it is called flash column chromatography. (flashcolumns.com)
  • Adsorption occurs on surfaces when solvent molecules from liquid become attracted to certain areas on its exterior surface. (ablogwithadifference.com)
  • The basic method for collection of carbon tetrachloride from the ambient atmosphere is adsorption on a solid phase, followed by removal by thermal or solvent elution for subsequent analysis. (cdc.gov)
  • The effects of dye-adsorption solvent on the performances of dye-sensitized solar cells based on phenothiazine dyes were investigated in this study. (hindawi.com)
  • Cell performance using EtOH as a dye-adsorption solvent showed relatively higher performance than that using THF. (hindawi.com)
  • Electrochemical and photochemical tests of phenothiazine dyes in solution and adsorbed on the TiO 2 surface showed less dye loading and coverage on the TiO 2 surface during adsorption in the case of THF, which decreased the solar cell performance of the DSSC using THF as adsorption solvent compared with using EtOH as adsorption solvent. (hindawi.com)
Analytical3
  • The polymers were detailed characterized by using series of analytical techniques, and their adsorption and recognition performance were validated by adsorption tests. (bvsalud.org)
  • The composition of the eluent flow can be monitored and each fraction is analyzed for dissolved compounds, e.g. by analytical chromatography, UV absorption spectra, or fluorescence. (wikipedia.org)
  • As is true for most volatile organic compounds, the preferred analytical technique for carbon tetrachloride is gas chromatography (GC). (cdc.gov)
Stationary phase5
  • The main advantage of column chromatography is the relatively low cost and disposability of the stationary phase used in the process. (wikipedia.org)
  • The stationary phase or adsorbent in column chromatography is a solid. (wikipedia.org)
  • Our Aluminium oxide Basic is a stationary phase that has been used in column chromatography for many years. (column-chromatography.com)
  • The classical preparative chromatography column, is a glass tube with a diameter from 5 mm to 50 mm and a height of 5 cm to 1 m with a tap and some kind of a filter (a glass frit or glass wool plug - to prevent the loss of the stationary phase) at the bottom. (blogspot.com)
  • Hydrophobic interaction chromatography (HIC) uses a filler with moderate hydrophobicity as the stationary phase and a salt-containing aqueous solution as the mobile phase, and uses the difference in the hydrophobic properties of the solute molecules to achieve separation by the difference in the strength of the hydrophobic interaction with the stationary phase, chromatographic method. (welch-us.com)
Substrate2
  • Film thickness and optical constants of an adsorption layer or oxide film on a substrate surface can be determined with exceptional sensitivity. (jascoinc.com)
  • Different methods of the LAL assay are available: the gel-clot assay, the chromogenic substrate method, and the turbidimetric method [ 10 ]. (springeropen.com)
Compounds7
  • A thin-layer chromatograph can show how a mixture of compounds will behave when purified by column chromatography. (wikipedia.org)
  • Size exclusion chromatography would normally be considered for analysing high molecular weight compounds (.2000). (pharmtech.com)
  • Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to effectively separate small polar compounds on polar stationary phases. (springer.com)
  • NP-LC has been widely used to separate various compounds, from nonpolar to highly polar compounds (note that chromatography was first introduced as a method used in separation science). (springer.com)
  • For example, it is suitable for analyzing compounds in complex systems that always elute near the void in reserved-phase chromatography. (springer.com)
  • Hydrophilic interaction liquid chromatography has established itself as the separation mode of choice for uncharged highly hydrophilic and amphiphilic compounds that are too polar to be well retained in RP-LC but have insufficient charge to allow effective electrostatic retention in ion-exchange chromatography. (springer.com)
  • Column chromatography in chemistry is a method used to purify individual chemical compounds from mixtures of compounds. (blogspot.com)
Substances6
  • Flash chromatography is a kind of fast chromatographic separation mode, which separates substances by using optimized pre-packaged medium and low-pressure columns. (flashcolumns.com)
  • Chemisorption and Physisorption are two forms of physical adsorption used to extract substances from a medium through interactions on solid surfaces. (ablogwithadifference.com)
  • Chemisorption (also referred to as chemical Adsorption) occurs when substances that adsorb onto an adsorbed substance are held there by chemical bonds formed during its attachment process. (ablogwithadifference.com)
  • Adsorption occurs via various mechanisms that depend on the properties and relationships between various substances that adsorb, such as chemical and physical . (ablogwithadifference.com)
  • Adsorption processes depend on many variables, including characteristics of both adsorbate and adsorbent as well as pressure, temperature, and shape of substances present within a system, plus environmental conditions like pressure or temperature and size/shape considerations. (ablogwithadifference.com)
  • HILIC also allows the analysis of charged substances, as in ion chromatography (IC). (springer.com)
Molecules3
  • The porous architecture allows adsorption of guest molecules with high efficiency, which is advantageous for rapid detection of the guest molecules. (nature.com)
  • Therefore, in the HIC process, a solution with high salt concentration is used in the sample adsorption stage, so that the target molecules are bound in the chromatographic column, and in the elution stage, the hydrophobicity between the solute and the chromatographic medium is made by reducing the salt concentration in the eluent. (welch-us.com)
  • At this download chemical, the Molecules on the microparticle are overrepresented by Acts in the gel-electrophoresis. (arm-sind-die-anderen.de)
Solvents1
  • The highest conversion efficiency of 3.78% was obtained using ethanol (EtOH) and 2.53% for tetrahydrofuran (THF), respectively, as dye-adsorption solvents. (hindawi.com)
Molecularly imprinted3
  • The results showed that the magnetic molecularly imprinted polymers possessed typically core-shell structure and exhibited class-specific recognition, fast adsorption saturation (only 1 min), and good magnetic separation performance towards targets. (bvsalud.org)
  • The scientists used a combination of dummy molecularly imprinted polymers (DMIPs) and molecularly imprinted polymer mixed matrix membranes (MMMs), and their findings were recently published in the Journal of Chromatography A (1). (chromatographyonline.com)
  • Wang, D. Dummy molecularly imprinted polymers-agarose gel mixed matrix membrane for extraction of amphetamine-type stimulants in wastewater and urine. (chromatographyonline.com)
Extraction1
  • For this issue, this study presents a comprehensive method for detecting bronopol content in aquaculture tissues using liquid chromatography-tandem mass spectrometry (LCâ MS/MS). The methodology was optimized, involving extraction with Cu-Zn precipitant, cleanup using a small HLB column, separation on a T3 column, and gradient elution with water and acetonitrile mobile phases. (bvsalud.org)
Eluent2
  • During the entire chromatography process the eluent is collected in a series of fractions. (wikipedia.org)
  • It is chosen so that the retention factor value of the compound of interest is roughly around 0.2 - 0.3 in order to minimize the time and the amount of eluent to run the chromatography. (wikipedia.org)
Chemistry1
Efficiency1
  • The remarkable specific area of 150 +- 10%m2/g allows for profound interactions with the analytes and optimizes separation efficiency. (column-chromatography.com)
Method6
  • More importantly, this developed method have good application potential in rapid analyzing field for neonicotinoids due to the amazing rapid adsorption time for extracting targets from complex food matrix (only 1 min). (bvsalud.org)
  • Flash column chromatography, also known as a flash column, is a fast separation method, but it does not sacrifice resolution as others have said. (flashcolumns.com)
  • Flash column chromatography is a fast and usually easy method to separate complex mixtures. (flashcolumns.com)
  • The method conditions are optimized using a two-level factorial design to achieve best purification conditions of the contaminated biologics and indicated endotoxin removal percentage 85.12% and maximum adsorption capacity of 38.5 mg/g, for histidine-coupled MNPs. (springeropen.com)
  • When absolute confidence in compound identity is required, gas chromatography/mass spectrometry (GC/MS) is the method of choice. (cdc.gov)
  • A composite of nano-zero valent iron (nZVI) supported on granular activated carbon (GAC) was synthesized through adsorption-reduction method, and its performances used as catalyst of Fenton-like were investigated to degrade methylene blue (MB) in model wastewater. (atlantis-press.com)
Chromatographic1
  • In theory, HIC and RPC are two closely related liquid chromatography techniques, both of which are based on the flow-water interaction between the hydrophobic regions on the surface of biomolecules and the hydrophobic ligands (alkyl or aromatic) on the chromatographic medium , however, the chromatographic mechanisms at the molecular level as well as the practical level differ between the two techniques. (welch-us.com)
Exchange2
Column11
Salt concentration1
  • Therefore, it is necessary to optimize the salt concentration during sample loading(ammonium sulfate is usually selected as the pretreatment salt for sample loading). (biolink.com)
Surfaces2
  • Physical Adsorption refers to the process by which gases such as nitrogen or hydrogen atoms adsorb onto surfaces like charcoal at lower temperatures, adhering themselves firmly. (ablogwithadifference.com)
  • To reduce the influence of CH2O adsorption at the surfaces, we realize a PA cell similar to that used in ref. 16, but with the inner walls of the cell gold coated. (gsk-3inhibitors.com)
Hydrophobicity1
  • The hydrophobicity of the hydrophobic ligand butyl-octyl-phenyl increases sequentially, and the hydrophobicity of the target product can be predicted through the combined adsorption test with different hydrophobic fillers. (biolink.com)
Liquid3
  • While gas or liquid chromatography-mass spectrometry are the techniques mostly used for quantifying ATSs, scientists still face challenges finding test methods that are rapid, efficient, involves minimal sample preparation, and can produce improved purification. (chromatographyonline.com)
  • Enhancing our understanding of retention behavior in HILIC increases the scope of possible applications of liquid chromatography. (springer.com)
  • Normal or reversed-phase liquid chromatography can be used for analysis. (springer.com)
Process4
  • Also referred to as physical adsorption, Physical Sorption is an exothermic process with minimal energy requirements for its operation - approximately 20-40kJ/mol of heat is typically required per mole. (ablogwithadifference.com)
  • Understanding the properties of the lignins increases the possibility to optimize the purification process. (lu.se)
  • In the downstream separation and purification process of biopharmaceuticals, chromatography is one of the indispensable separation methods. (biolink.com)
  • How To Select The Appropriate Chromatography Resin For My Own Process? (biolink.com)
Widely1
  • And it is a fast and inexpensive technology compared with traditional chromatography, which is widely used in drug research, sample purification, and purification of natural products currently. (flashcolumns.com)
Methods1
Solid1
  • Semi-solid (gels) or solid formulations (freeze-dried tablets with controlled release) were also developed. (europa.eu)
Conditions1
  • AndroGel is a transdermally administered gel prescribed to men with abnormally low testosterone levels ("hypogonadism") associated with various medical conditions. (mentalhealthdaily.com)
Surface2
  • The stationary phases are usually finely ground powders or gels and/or are microporous for an increased surface, though in EBA a fluidized bed is used. (wikipedia.org)
  • The optimized pore structure and surface area of the product enhance purification. (column-chromatography.com)
Sample1
  • A multi-layer film model is developed for the sample, the film thickness and optical constants are optimized to minimize the error for the measured values. (jascoinc.com)
Time1
Magnetic1
  • This work described a proof of concept study of hybrid nanogel-based magnetic resonance contrast agents, SPIO@GCS/acryl/biotin@Mn-gel, abb. (duke.edu)
Article1
  • This article provides testing instructions for the piston-operated pipette BRAND Transferpette® according to the standard DIN EN ISO 8655. (sigmaaldrich.com)
Chemical1
  • So, the download chemical of number of any liver through the seizure spins on its T for the pH gemeinsamen, its paper of oxidation, and the Chromatography and camera of complicating results in the comparison. (arm-sind-die-anderen.de)
Separate1
Medium1
  • The RPC medium can be considered as a continuous hydrophobic phase, and the degree of substitution of ligands such as C4~C18 alkyl groups is usually in the hundreds of micromoles/mL gel: while the HIC medium has ligands such as C2~C alkyl groups or simple aromatic groups. (welch-us.com)