• We described how Streptomyces spores hitch a ride by sticking to Bacillus subtilis flagella for local journeys or by emitting geosmin to attract springtails they are transported to faraway lands . (asmblog.org)
  • To that end, the authors tested the interactions between nano-C 60 and two common laboratory bacteria, E. coli DH5α, a Gram-negative bacterium, and Bacillus subtilis , a Gram-positive bacterium. (medscape.com)
  • Natto, a traditional soy food fermented by Bacillus subtilis (natto), is made by steaming or cooking soaked soybean seeds, inoculating them with the bacteria, and then letting them sit for an incubation period. (researchgate.net)
  • Spontaneous Bacillus anthracis mutants resistant to infection by phage AP50c (AP50R) exhibit a mucoid colony phenotype and secrete an extracellular matrix. (nih.gov)
  • The revelation in May 2015 of the shipment of γ irradiation-inactivated wild-type Bacillus anthracis spore preparations containing a small number of live spores raised concern about the safety and security of these materials. (cdc.gov)
  • Wip1 is a recently isolated phage that infects Bacillus anthracis, the notorious biothreat pathogen and gram-positive bacterium that causes anthrax disease. (rockefeller.edu)
  • The current standard for identifying suspected B. anthracis involves testing for Wγ phage sensitivity. (rockefeller.edu)
  • The findings that we report here also demonstrate that Wip1 phage and its receptor-binding ligand p23 are promising, new tools in the detection of B. anthracis. (rockefeller.edu)
  • This report describes a 49-year-old male construction worker who acquired a Bacillus anthracis infection after working on a sheep farm. (cdc.gov)
  • Bacillus anthracis has traditionally been considered the etiologic agent of anthrax. (cdc.gov)
  • Here we utilized a Roche/454-based whole genome sequencing approach to identify mutations that are candidates for conferring AP50c phage resistance, followed by genetic deletion and complementation studies to validate the whole genome sequence data and demonstrate that the implicated gene is necessary for AP50c phage infection. (nih.gov)
  • Infection and transformation BACTERIAL MUTATION STUDIES Phage resistance mutants-Quantitative mutation studies are easiest when the mutants can be counted by inspection. (nih.gov)
  • Conventionally, phage therapy relies on the use of naturally-occurring phages to infect and lyse bacteria at the site of infection. (wjgnet.com)
  • Although patients with tuberculosis also generate larger particles containing numerous bacilli, these particles do not serve as effective vehicles for transmission of infection because they do not remain airborne, and if inhaled, do not reach alveoli. (cdc.gov)
  • BACTERIOPHAGE T4), and T6, and the phage T5 are called "autonomously virulent" because they cause cessation of all bacterial metabolism on infection. (lookformedical.com)
  • Significant GrSRs were observed with the presences of host genes in promoting the infection cycle at stages of adsorption, establishment, and viral release, but nonsignificant with the presence of immune systems, such as restriction-modification systems and CRISPR-Cas systems. (biomedcentral.com)
  • The following two species are assigned to the genus: Bacillus virus Camphawk Bacillus virus SPO1 Viruses in Okubovirus are non-enveloped, with head-tail geometries, and T=16 symmetry. (wikipedia.org)
  • Myoviridae were assigned to the order Caudovirales in 1998 SPO1-like phages were renamed SPO1-like viruses in 1999 Bacillus phage SP8 was merged into Bacillus phage SPO1 in 1999 (as type species) of SPO1-like viruses. (wikipedia.org)
  • The following genera and species are recognized: Beecentumtrevirus Bacillus phage Nf Bacillus virus B103 Bacillus virus Goe1 Salasvirus Bacillus virus Goe6 Bacillus virus Gxv1 Bacillus virus phi29 Bacillus virus PZA Viruses in Picovirinae are non-enveloped, with icosahedral or prolate heads of about 50-55 nm in diameter, and short tails. (wikipedia.org)
  • The practice of phage therapy, which uses bacterial viruses (phages) to treat bacterial infections, has been around for almost a century. (wjgnet.com)
  • Viruses whose host is Bacillus. (lookformedical.com)
  • A temperate inducible phage and type species of the genus lambda-like viruses, in the family SIPHOVIRIDAE. (lookformedical.com)
  • Second, high-host density stimulated by a high-growth rate cloud provide more chances of host cells encountering more virus species and enhances the virus adsorption rate [ 20 ], which may increase the probability in coinfection or even superinfection with diverse viruses in the host and generally modelled by Kill-the-Winner dynamic [ 21 ]. (biomedcentral.com)
  • By mixing the E. coli phage PHH01 with motile B. cereus in a flow cell, phage reached and infected the E. coli in the submerged biofilm more effectively that in the absence of B. cereus . (asmblog.org)
  • In addition, when mixed with the phage, B. cereus colonized the preformed E. coli biofilm more effectively than in the absence of phage. (asmblog.org)
  • High over the biofilm they flow a solution that can contain either Bacillus cereus , the coliphage PHH01, or a mixture of both. (asmblog.org)
  • But when phage and B. cereus are mixed before entering the chamber, both reach the biofilm. (asmblog.org)
  • However, anthrax-like illness has been documented in welders and other metal workers infected with Bacillus cereus group spp. (cdc.gov)
  • double-stranded dna phages of both gram-positive and gram-negative bacteria typically use a holin-endolysin system to achieve lysis of their host. (liverpool.ac.uk)
  • in this study, the lysis genes of staphylococcus aureus phage p68 were characterized. (liverpool.ac.uk)
  • Biotechnological advances have further expanded the repertoire of potential phage therapeutics to include novel strategies using bioengineered phages and purified phage lytic proteins. (wjgnet.com)
  • Current research on the use of phages and their lytic proteins against multidrug-resistant bacterial infections, suggests phage therapy has the potential to be used as either an alternative or a supplement to antibiotic treatments. (wjgnet.com)
  • Bacillus phage SPO1 was assigned to the genus SPO1-like phages in 1996 as type species. (wikipedia.org)
  • As expected from these results, a targeted deletion or frame-shift mutations introduced into csaB in a different genetic background, in a strain not exposed to AP50c, resulted in a phage resistant phenotype. (nih.gov)
  • Most bacteriophages interact with bacterial surfaces using tail fibers, but Wip1 is a tailless phage possessing an icosahedral protein coat covering an internal lipid membrane. (rockefeller.edu)
  • Account must be taken, however, of genetic variations in the phage stocks used (6). (nih.gov)
  • The chemical nature of the phage-inactivating agent in bacterial extracts. (nih.gov)
  • The phenomenon by which a temperate phage incorporates itself into the DNA of a bacterial host, establishing a kind of symbiotic relation between PROPHAGE and bacterium which results in the perpetuation of the prophage in all the descendants of the bacterium. (lookformedical.com)
  • Observations on phage inhibition by bacillary extracts. (nih.gov)
  • i- ede 2 LEDERBERG for resistance to bacteriophages have many virtues, as the number of resistant mutants in a population is estimated by plate counts of the survivors of phage attack. (nih.gov)
  • a phage lambda vector with a q(-) mutation was used to enhance the expression of the lambda system. (liverpool.ac.uk)
  • These features indicate that Wip1 belongs to the family Tectiviridae, a relatively rare phage group with a proposed evolutionary lineage to the mammalian adenovirus. (rockefeller.edu)
  • And, at high flow rates, when alone the phages do not reach the biofilm - they are washed out through the outlet of the chamber. (asmblog.org)