• Scholars@Duke publication: beta2-Adrenergic receptor regulation by GIT1, a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein. (duke.edu)
  • We have identified a novel G protein-coupled receptor kinase-interacting protein, GIT1, that is a GTPase-activating protein (GAP) for the ADP ribosylation factor (ARF) family of small GTP-binding proteins. (duke.edu)
  • The encoded protein is a small GTPase that contains both N-terminal and C-terminal guanine nucleotide-binding motifs. (nsjbio.com)
  • Canis lupus familiaris ADP ribosylation factor like GTPase 3 (ARL3), mRNA. (genscript.com)
  • Putative zinc fingers with GTPase activating proteins (GAPs) towards the small GTPase, Arf. (embl.de)
  • Classical GAP proteins accelerate GTPase activity by supplying an arginine finger to the active site. (embl.de)
  • Molecular characterization of the GTPase-activating domain of ADP-ribosylation factor domain protein 1 (ARD1). (embl.de)
  • Both guanine nucleotide-exchange proteins and GTPase-activating proteins (GAPs) for ARFs have been cloned recently. (embl.de)
  • Like Ras, ARFs are active in their GTP-bound form, and their duration of activity is controlled by GTPase-activating proteins (GAPs), which assist ARFs in hydrolyzing GTP to GDP. (embl.de)
  • However, unlike ARFs, ARL4 does not activate the cholera toxin ADP-ribosyltranferase. (prospecbio.com)
  • Arfs accomplish this task through modification of membrane lipids and the recruitment of proteins, including coat proteins and actin, to membrane surfaces. (portlandpress.com)
  • ADP-ribosylation factors (ARFs) are approximately 20-kDa guanine nucleotide-binding proteins recognized as critical components in intracellular vesicular transport and phospholipase D activation. (embl.de)
  • ADP ribosylation factors (ARFs), which are members of the Ras superfamily of GTP-binding proteins, are critical components of vesicular trafficking pathways in eukaryotes. (embl.de)
  • Gene encoding ADP-ribosylation factor and similar to other ARFs and ARF-like proteins. (or.jp)
  • ADP-ribosylation factor 1 is a protein that in humans is encoded by the ARF1 gene. (wikipedia.org)
  • ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. (wikipedia.org)
  • The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. (wikipedia.org)
  • The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. (wikipedia.org)
  • We show that the ubiquitous trafficking protein ADP Ribosylation Factor 1 (ARF1) and the hemocyte-specific endosomal regulator Asrij differentially regulate humoral immunity. (nih.gov)
  • In the present study, we identified ADP-ribosylation factor 1 (ARF1) contributed to the inhibitory effect of GBV-C E2 on HIV-1 Gag membrane targeting. (oncotarget.com)
  • In addition, GBV-C E2 expression also altered Golgi morphology and suppressed protein traffic through the secretory pathway, which are all consistent with a phenotype of disrupting the function of ARF1 protein. (oncotarget.com)
  • Arf1 and Arf6 are the most divergent and most studied human Arf proteins that localize predominantly to the Golgi complex and plasma membrane respectively. (portlandpress.com)
  • Arf1 can also stimulate the activity of phosphatidylinositol kinases and recruit coat proteins and actin cytoskeletal elements to the Golgi complex. (portlandpress.com)
  • Transport vesicles can move molecules between locations inside the cell, e.g., proteins from the rough endoplasmic reticulum to the Golgi apparatus . (wikipedia.org)
  • Most of these proteins mature in the Golgi apparatus before going to their final destination which may be to lysosomes , peroxisomes , or outside of the cell. (wikipedia.org)
  • Donaldson, J. G., Finazzi, D. & Klausner, R. D. Brefeldin A inhibits Golgi membrane-catalysed exchange of guanine nucleotide onto ARF protein. (nature.com)
  • The GTP-bound form of ARF is essential for the maintenance of normal Golgi morphology, it participates in recruitment of coat proteins which are required for budding and fission of membranes. (embl.de)
  • The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. (nih.gov)
  • The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. (nih.gov)
  • Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. (nih.gov)
  • They occur in a large number of functionally diverse proteins mainly from eukaryotes. (embl.de)
  • We have identified a novel pathway that directs Lyn/Src family tyrosine kinase signals to the invadopodia to regulate sarcoma cell invasion via the molecule AFAP-1-like-1 (AFAP1L1), a new member of the AFAP (actin filament-associated protein) family. (nature.com)
  • A member of GTP-binding protein of the large ras superfamily involved in regulation of membrane trafficking by modulating ENDOCYTOSIS and ACTIN CYTOSKELETON remodeling. (bvsalud.org)
  • sigma-adaptin subunit of adaptor protein complex 3 (AP-3) status:Confirmed UniProt:Q8T874 protein_id. (yeastrc.org)
  • Adaptor complexes medium subunit family status:Partially_confirmed UniProt:P90761 protein_id:CAB0279. (yeastrc.org)
  • The product of this gene is an epsilon subunit of coatomer protein complex. (nih.gov)
  • During growth, genes associated with protein ubiquitination/deubiquitination, cell cycle progression, and proteasomal degradation were affected, while genes linked to protein and carbohydrate catabolism were affected during early development. (frontiersin.org)
  • Overall, this study reveals the impact of cln5 -deficiency on gene expression in D. discoideum , provides insight on the genes and proteins that play a role in regulating Cln5-dependent processes, and sheds light on the molecular mechanisms underlying CLN5 disease. (frontiersin.org)
  • Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that the candidate reference genes were significantly enriched in cellular metabolic process, protein metabolic process and ribosome in early development and normal adult tissues as well as in cellular localization and endocytosis in the hindgut under sulfide stress. (biomedcentral.com)
  • In the online version, these sections contain links to more information about proteins encoded by over 17,000 known or predicted human genes. (cshlpress.com)
  • Analyzing 5′-upstream non-protein-encoding regions of the human mitochondrial function-associated genes, we speculate that mitochondrial functions could be recovered or improved at a transcriptional level. (intechopen.com)
  • Béthune, J. and Wieland, F.T. (2018) Assembly of COPI and COPII Vesicular Coat Proteins on Membranes. (haw-hamburg.de)
  • Moxetumomab pasudotox-tdfk internalization results in ADP-ribosylation of elongation factor 2, inhibition of protein synthesis, and apoptotic cell death. (geisinger.org)
  • Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. (wikipedia.org)
  • Strong similarity to gbM95166 ADP-ribosylation factor from Arabidopsis thaliana. (cornell.edu)
  • These sequences represent the protein coding region of the ARL3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. (genscript.com)
  • The entire text of the Guide is searchable, and tools are available for identifying human protein sequences using those from other species. (cshlpress.com)
  • Recombinant human protein (amino acids R16-S428) was used as the immunogen for the ARL13B antibody. (nsjbio.com)
  • 1991). "Human ADP-ribosylation factors. (wikipedia.org)
  • Amyloid plaques and neighbouring non-plaque tissue were microdissected from human brain sections using laser capture microdissection and label-free LC-MS was used to quantify the proteins present. (researchsquare.com)
  • Ranibizumab is a recombinant humanized monoclonal antibody fragment which binds to and inhibits human vascular endothelial growth factor A (VEGF-A). Ranibizumab inhibits VEGF from binding to its receptors and thereby suppressing neovascularization and slowing vision loss. (geisinger.org)
  • Luxturna (voretigene-neparvovec-rzyl) is an adeno-associated virus vector-based gene therapy that delivers a normal copy of the gene encoding human retinal pigment epithelial 65 kDa protein (RPE65) to retinal cells thus augmenting reduced or absent levels of biologically active RPE65. (geisinger.org)
  • For each protein, basic characteristics about its composition and length, its human relatives and relatedness to proteins in other species, and direct links to resources at NCBI are included. (cshlpress.com)
  • Additional links to NCBI resources are provided for human noncoding RNAs and repeated DNA elements and for proteins of interest from other species. (cshlpress.com)
  • Human cytomegalovirus G protein-coupled receptor US28 promotes latency by attenuating c-fos. (uc.edu)
  • An intriguing aspect of protein self-assembly is the formation of amyloid fibrils, underlying several human diseases. (lu.se)
  • These proteins contain a characteristic zinc finger motif (Cys-x2-Cys-x(16,17)-x2-Cys) which displays some similarity to the C4-type GATA zinc finger. (embl.de)
  • The ARFGAP domain display no obvious similarity to other GAP proteins. (embl.de)
  • G protein-coupled receptor activation leads to the membrane recruitment and activation of G protein-coupled receptor kinases, which phosphorylate receptors and lead to their inactivation. (duke.edu)
  • Moreover, they provide a mechanism for integration of receptor activation and endocytosis through regulation of ARF protein activation by GRK-mediated recruitment of the GIT1 ARF GAP to the plasma membrane. (duke.edu)
  • GB virus type C (GBV-C) glycoprotein E2 protein disrupts HIV-1 assembly and release by inhibiting Gag plasma membrane targeting, however the mechanism by which the GBV-C E2 inhibits Gag trafficking remains unclear. (oncotarget.com)
  • Membrane-bound and secreted proteins are made on ribosomes found in the rough endoplasmic reticulum . (wikipedia.org)
  • Recently, ARMER, a novel integral ER-membrane protein was shown to protect cells from ER stress-induced apoptosis. (thermofisher.com)
  • To solve this problem, the cell membrane contains proteins that are selective for unique, water soluble molecules. (wikibooks.org)
  • The availability of free energy is one of the factors that determine if a molecule will move across a membrane, the other being the permeability of the molecule in the lipid bilayer. (wikibooks.org)
  • Arf (ADP-ribosylation factor) GTP-binding proteins function in cells to regulate membrane traffic and structure. (portlandpress.com)
  • Pertussis toxin B-pentamer mediates intercellular transfer of membrane proteins and lipids. (uc.edu)
  • Around 60-80% of cases1 are caused by mutations of the gene that encodes optic atrophy protein 1 (OPA1), a protein that has a key role in inner mitochondrial membrane fusion and remodelling of cristae and is crucial for the dynamic organization and regulation of mitochondria2. (bvsalud.org)
  • While endosomal regulation of Drosophila hematopoiesis is reported, the role of endosomal proteins in cellular and humoral immunity is not well-studied. (nih.gov)
  • The results of recent studies demonstrate that ADP-ribosylation contributes to transcription regulation. (elsevierpure.com)
  • Furthermore, we study the functional consequences of protein-ligand interactions and the regulation of these interactions. (lu.se)
  • This gene encodes a member of the ADP-ribosylation factor-like family. (nsjbio.com)
  • Stimulation of Gi-coupled receptors leads to the activation of mitogen-activated protein kinases (MAP kinases). (duke.edu)
  • The beta-adrenergic receptor kinase 1 (beta ARK1) is a member of the G protein-coupled receptor kinase (GRK) family that mediates the agonist-dependent phosphorylation and desensitization of G protein-coupled receptors. (duke.edu)
  • We are primarily using cytomegaloviruses to examine how the pathogens alter signaling pathways directed by G-protein coupled receptors (GPCRs) to facilitate robust replication in tissues important for host-host dissemination. (uc.edu)
  • ADP-ribosylation is a reversible posttranslational modification mediated by poly-ADP-ribose polymerase (PARP). (elsevierpure.com)
  • Here, we report that transcription factor NFAT binds to and is ADP-ribosylated by PARP-1 in an activation-dependent manner. (elsevierpure.com)
  • Together, these results demonstrate that ADP-ribosylation mediated by PARP-1 provides a molecular switch to positively regulate NFAT-dependent cytokine gene transcription. (elsevierpure.com)
  • We had reported that the ARF domain of ARD1 binds specifically GDP and GTP and that the amino-terminal extension acts as a GAP for the ARF domain of ARD1 but not for ARF proteins. (embl.de)
  • PAPbeta, a protein that binds to and is phosphorylated by the non-receptor tyrosine kinase PYK2, contains several modular signaling domains including a pleckstrin homology domain, an SH3 domain, ankyrin repeats and an ARF-GAP domain. (embl.de)
  • Here we demonstrate a functional role for endosomal proteins in immune homeostasis. (nih.gov)
  • Our study highlights the role of endosomal proteins in modulating the immune response by maintaining the balance of AMP production. (nih.gov)
  • Endosomal/lysosomal proteins were particularly highly enriched in amyloid plaques. (researchsquare.com)
  • Fluorescent immunohistochemistry was used to validate the enrichment of four proteins in amyloid plaques (moesin, ezrin, ARL8B and SMOC1) and to compare the amount of total Aβ, Aβ40, Aβ42, phosphorylated Aβ, pyroglutamate Aβ species and oligomeric species in EOAD and DS. (researchsquare.com)
  • AFAP1L1 is a novel adaptor protein of the AFAP family that interacts with cortactin and localizes to invadosomes. (nature.com)
  • It should be noted that ARMER is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains. (thermofisher.com)
  • Jackson, C. L. & Casanova, J. E. Turning on ARF: the Sec7 family of guanine-nucleotide-exchange factors. (nature.com)
  • We have previously shown that second-messenger-dependent kinases (cAMP-dependent kinase, protein kinase C) in the olfactory system are essential in terminating second-messenger signaling in response to odorants. (duke.edu)
  • These results suggest an essential role for ARF proteins in regulating beta2-adrenergic receptor endocytosis. (duke.edu)
  • Direct evidence that Gi-coupled receptor stimulation of mitogen-activated protein kinase is mediated by G beta gamma activation of p21ras. (duke.edu)
  • Functional desensitization of the isolated beta-adrenergic receptor by the beta-adrenergic receptor kinase: potential role of an analog of the retinal protein arrestin (48-kDa protein). (duke.edu)
  • Morinaga, N., Tsai, S.-C., Moss, J. & Vaughan, M. Isolation of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP ribosylation factor (ARF) 1 and ARF3 that contains a Sec7-like domain. (nature.com)
  • Lysine acetylation targets protein complexes and co-regulates major cellular functions. (nih.gov)
  • Amaya Ramirez C., Egetemaier S. and Béthune J. (2021) Context-Specific and Proximity-Dependent Labeling for the Proteomic Analysis of Spatiotemporally Defined Protein Complexes with Split-BioID. (haw-hamburg.de)
  • Schopp, I.M. and Béthune, J. (2018) Split-BioID - Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment. (haw-hamburg.de)
  • Analyzing context-specific protein complexes. (haw-hamburg.de)
  • ARL4A is related Specifically to ARL6 and ARL7 and belongs to the ARF-like protein (ARL) subfamily of small GTPases. (prospecbio.com)
  • Cherfils, J. & Chardin, P. GEFs: structural basis for their activation of small GTP-binding proteins. (nature.com)
  • They are involved in protein-protein interactions. (embl.de)
  • Protein folding and assembly are processes governed by a large number of non-covalent protein-protein interactions as well as interactions between the proteins and their environment. (lu.se)
  • Within this area of research we study the relative contribution of different types of non-covalent interactions to protein folding and oligomerisation. (lu.se)
  • Amyloid plaques contain many proteins in addition to beta amyloid (Aβ). (researchsquare.com)
  • they provide insight into the factors that drive amyloid plaque development and are potential biomarkers or therapeutic targets for Alzheimer's disease (AD). (researchsquare.com)
  • The aim of this study was to comprehensively identify proteins that are enriched in amyloid plaques using unbiased proteomics in two subtypes of early onset AD: sporadic early onset AD (EOAD) and Down Syndrome (DS) with AD. (researchsquare.com)
  • We focused our study on early onset AD as the drivers of the more aggressive pathology development in these cases is unknown and it is unclear whether amyloid-plaque enriched proteins differ between subtypes of early onset AD. (researchsquare.com)
  • 48 proteins were consistently enriched in amyloid plaques in EOAD and DS. (researchsquare.com)
  • The most enriched proteins in amyloid plaques in both EOAD and DS were: COL25A1, SMOC1, MDK, NTN1, OLFML3 and HTRA1. (researchsquare.com)
  • Overall, we observed that amyloid plaques in EOAD and DS largely contained the same proteins, however the amount of enrichment of some proteins was different in EOAD and DS. (researchsquare.com)
  • Our study highlights the significant enrichment of many proteins in amyloid plaques, many of which may be potential therapeutic targets and/or biomarkers for AD. (researchsquare.com)
  • Amyloid plaques are a neuropathological hallmark of Alzheimer's disease and primarily consist of the protein beta amyloid (Aβ). (researchsquare.com)
  • However, it is often overlooked that amyloid plaques also contain hundreds of proteins in addition to Aβ. (researchsquare.com)
  • Therefore, comprehensively profiling the proteins that are enriched in amyloid plaques would increase our understanding about AD pathogenesis, and possibly identify new biomarkers and/or new therapeutic targets for AD. (researchsquare.com)
  • Previous studies have typically used immunohistochemistry to identify amyloid plaque proteins. (researchsquare.com)
  • Mass spectrometry-based proteomics is an alternative approach that allows efficient quantification of thousands of amyloid plaque proteins simultaneously. (researchsquare.com)