MSCsADSCsTransplantationRegenerationHarvested from adipose tissueASCsAutologousDifferentiationOsteogenicStromal vascular fraction cellsSubcutaneous adiposeCanine adiposeVascularOsteogenesisOsteoarthritisAdultRegenerativeVitro and in vivoProliferationTissuesCryopreservationHypoxiaDifferentiate into variousScaffoldsProgenitorInjectionsExosomesBone tissueADSCADMSCsViabilityLumpsInflammatoryPotentialCytokinesEmbryonicCartilageInjectionClinicalInflammationAbdominal
MSCs19
- Adipose tissue (AT) represents a commonly used source of mesenchymal stem/stromal cells (MSCs) whose proregenerative potential has been widely investigated in multiple clinical trials worldwide. (mdpi.com)
- In recent years, one of the main research strategies used to induce angiogenesis in vivo has been via stem cell therapy, especially therapy using mesenchymal stem cells (MSCs). (minervamedica.it)
- Therefore, this study aimed to investigate the effects of hypoxia-preconditioned MSCs from human adipose tissue-derived MSCs (AT-MSCs) and from human umbilical cord-derived MSCs (UC-MSCs). (minervamedica.it)
- METHODS: Both AT-MSCs and UC-MSCs were obtained from an established cell bank and expanded in vitro . (minervamedica.it)
- The concentrations of growth factors (such as HGF, bFGF, and VEGF) in the cell culture supernatants collected from culture media of the hypoxia-preconditioned AT-MSCs and UC-MSCs (as well as those from AT-MSCs and UC-MSCs cultured in normoxia) were measured by ELISA. (minervamedica.it)
- Adipose tissue-derived stem cells display multi-lineage developmental plasticity, very much like bone marrow-derived mesenchymal stromal cells (BM-MSCs), and can differentiate into several cell lines, including adipocytes, chondrocytes and osteoblasts, as well as endothelial and neural lineages. (dermatologytimes.com)
- Mesenchymal stem cells (MSCs) derived from adipose are widely used as a source of stem cells. (unair.ac.id)
- It is hoped that the survival, engraftment, and function of implanted MSCs by preconditioning the cells will be improved. (unair.ac.id)
- Compared with mesenchymal stem cells (MSCs) derived from other tissues such as umbilical cord and bone marrow, isolation of ASCs from human white adipose tissue (WAT) has great advantages due to its rich tissue source and simple surgical procedure. (edu.au)
- About this last point, a ray of hope comes from the potential of Mesenchymal Stem Cells (MSCs) that has already been established in Coronavirus Disease 2019 (COVID-19), and in particular from the Adipose-Derived Mesenchymal Stem Cells (AD-MSCs). (torvergata.it)
- In a randomized, double-blinded, controlled clinical trial [ 3 ], 24 % of a total of 55 patients who underwent a partial medial meniscectomy followed by intra-articular (IA) injection with 5-15 × 10 7 allogeneic bone marrow-derived MSCs achieved a 15 % increase in the meniscal volume determined by magnetic resonance imaging (MRI) at the 2-year follow-up, suggesting evidence of meniscus regeneration after MSC treatment. (biomedcentral.com)
- Another proof-of-concept clinical trial in which OA patients ( n = 9) treated with IA injection of 10 × 10 7 adipose-derived autologous MSCs demonstrated improved Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) scores and regeneration of hyaline-like cartilage in the affected joint [ 4 ]. (biomedcentral.com)
- Recently, allogeneic bone marrow-derived MSCs were also used in an OA clinical trial. (biomedcentral.com)
- Adipose-derived stem cells (ASCs) are a readily available population of mesenchymal stem/stromal cells (MSCs) that can be isolated within minimally invasive surgery. (upmbiomedicals.com)
- Recently, mesenchymal stem cells (MSCs) have been confirmed as a potential treatment in kidney regeneration. (tmu.edu.tw)
- Mesenchymal stem cells (MSCs) have been studied for their potential benefits in treating acute respiratory distress syndrome (ARDS) and have reported mild effects when trialed within human clinical trials. (lu.se)
- Human integrin α10β1-selected adipose tissue-derived MSCs (integrin α10β1-MSCs) have shown immunomodulatory and regenerative effects in various disease models. (lu.se)
- We hypothesized that integrin α10β1 selected-MSCs can be used to treat a sepsis-induced ARDS in a porcine model when administering cells after established injury rather than simultaneously. (lu.se)
- 6 cells/kg integrin α10β1-selected MSCs and both groups were followed for 12 h. (lu.se)
ADSCs19
- In the present study, the potential of using exosomes isolated from adipose‑derived stem cells (ADSCs) as a therapeutic tool for reducing chronic inflammation and promoting chondrogenesis was investigated using patient‑derived primary cells. (spandidos-publications.com)
- First, it was tested whether patient‑derived ADSCs could differentiate into chondrogenic and osteogenic lineages. (spandidos-publications.com)
- The present study provided evidence in support for the use of patient‑derived exosomes, produced from ADSCs, for potential chondrogenic regeneration and subsequent amelioration of osteoarthritis. (spandidos-publications.com)
- Adipose tissue-derived stem cells (ADSCs) have been characterized as having the ability to self-renew and differentiate into different connective tissue cells, including osteoblasts, adipocytes, chondrocytes and myocytes, under specific inductive stimuli ( 4 ). (spandidos-publications.com)
- In total, 1-10% of nucleated cells in adipose tissue are ADSCs, whereas only 0.0001-0.01% of nucleated cells in bone marrow are stem cells ( 5 ). (spandidos-publications.com)
- Most clinical trials which utilize ADSCs for OA treatment have been based on the autologous cells from the stromal vascular fraction, as have most of the in vivo studies ( 6 , 7 ). (spandidos-publications.com)
- Furthermore, ADSCs are known to have various cytokine-secreting properties and beneficial paracrine effects on surrounding cells or tissues through secretion of multiple angiogenic and antiapoptotic growth factors, including vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), basic fibroblast growth factor (bFGF) and platelet-derived growth factor (PDGF). (dermatologytimes.com)
- By modulating the cell cycle and activating the anagen phase in the hair cycle, the application of the ADSCs appear to promote hair growth by increasing the proliferation of the dermal papilla cells and possibly the HaCaT epithelial cells. (dermatologytimes.com)
- We noticed less of an influence of the ADSCs on the proliferation of HaCaT cells and organ culture, with relatively narrow therapeutic concentrations of ADSC-CM,' Dr. Kim says. (dermatologytimes.com)
- In previous studies, Dr. Kim identified secretory factors derived from ADSCs by ELISA and proteomic analysis, including several factors related to IGF binding protein precursors, PDGF, bFGF, keratinocyte growth factor (KGF), HGF, VEGF and fibronectin. (dermatologytimes.com)
- More recently, stem cells collected from adipose tissue (adipose-derived stem cells or ADSCs) have gained traction due to their relative ease of collection and differentiation potential. (psu.edu)
- Both undifferentiated and Schwann cell-like differentiated ADSCs have been used to seed conduits with variable results. (psu.edu)
- Adipose-derived stem cells (ADSCs) are the focus of this post, since they have been the subject of numerous research papers in the hair growth field. (hairlosscure2020.com)
- We investigated in vitro the paracrine effects of human adipose-derived stem cells (ADSCs) on wound healing mechanisms for human tympanic membrane-derived keratinocytes (hTM) and immortalized human keratinocytes (HaCaT). (oslomet.no)
- Together with gene regulation associated with hypoxia in ADSCs, this study has provided several strong leads for a stem cell-derived approach to TM wound healing. (oslomet.no)
- In this study we tracked i.c.-injected adipose-derived stem cells (ADSCs) in various tissues. (stemcellclinicsofamerica.net)
- Adipose-derived stem cells (ADSCs) have generated great interest in recent years because of their multipotency and ease of harvest, and they have already been verified to differentiate into Schwann-like cells (SLCs) in vitro. (tokushima-u.ac.jp)
- Intravenous adipose mesenchymal stem cells (ADSCs) attenuate renal ischemia/reperfusion (IR) injury but with major drawbacks, including the lack of a specific homing effect after systemic infusion, cell trapping in the lung, and early cell death in the damaged microenvironment. (ntnu.edu.tw)
- The stromal vascular fraction from abdominal adipose tissue was subcultured for ten passages to enrich for and expand the number of ADSCs. (vinastemcelllab.com)
Transplantation7
- Autologous transplantation of hADSCs is only the best choice in ages that these cells have the optimum efficacy. (ac.ir)
- Improvement of this method may help the formation of an unlimited source of cells for transplantation. (celljournal.org)
- As of 2016[update], the only established medical therapy using stem cells is hematopoietic stem cell transplantation, first performed in 1958 by French oncologist Georges Mathé. (wikipedia.org)
- By 1998, human embryonic stem cells were first isolated by American biologist James Thomson, which made it possible to have new transplantation methods or various cell types for testing new treatments. (wikipedia.org)
- She contributed as a speaker at global webinar on stem cell during November 11-12-2021, she also attended the BIT's 10th World Congress of Regenerative Medicine & Stem Cells -2016 in China and gave a talk titled as ' Bone Marrow Mesenchymal Stem Cell Transplantation in a Rabbit Corneal Alkali Burn Model . (magnusconferences.com)
- We examined whether intrarenal arterial transplantation of dexmedetomidine (DEX) preconditioning ADSC-derived microvesicles (DEX-MVs) could promote further therapeutic potential to reduce renal IR injury. (ntnu.edu.tw)
- Another form of lipodystrophy that fits the classification of "acquired partial" not involving the complement pathway is associated with hematopoietic stem cell transplantation (HSCT) to treat leukemia or neuroblastoma. (medscape.com)
Regeneration12
- When dissociated, they can be co-cultured with SC-like adipose-derived stem cells (ASC), providing a valuable model to study in vitro nerve regeneration and myelination, mimicking the in vivo environment at the injury site. (jove.com)
- Strategies for regeneration of the bone using porcine adult adipose-derived mesenchymal stem cells. (oregonstate.edu)
- Bone marrow mesenchymal stem cells (BMSC) have been the choice, thus far, for stem cell therapy for bone regeneration. (oregonstate.edu)
- Stem cell-based therapy is one of the most promising strategies in promoting tissue regeneration. (unair.ac.id)
- Treatment using adipose-derived stem cell-conditioned medium appears highly effective for alopecia and may represent a new therapy for hair regeneration. (hairlosscure2020.com)
- Induced osteogenesis of adipose-derived mesenchymal stem cells (AMSCs) has been used to facilitate bone regeneration. (aging-us.com)
- This will allow us to better understand the causal relationship between Ca2+ dynamics and differentiation, potentially leading to the development of novel, more effective interventions for both bone regeneration and control of adipose growth. (agu.edu.tr)
- Schwann cells (SCs) are expected to be applied in cell therapy because of their ability to promote peripheral nerve regeneration. (tokushima-u.ac.jp)
- These findings demonstrated that the potential of ADSC-derived SLCs to promote nerve regeneration could be significantly increased using our modified differentiation protocol and by assembling cells into a 3D sphere conformation. (tokushima-u.ac.jp)
- Adipose-derived stem cells have been shown to enhance bone healing and regeneration in numerous experimental models . (bvsalud.org)
- The adipose tissue is an important reservoir of adult stem cells which have capacity of differentiating in osteoblasts with potential implication in reaching bone regeneration. (bvsalud.org)
- Tissue bioengineering is a new branch of Science aiming to investigate and integrate techniques of tissue regeneration thorugh new biomaterials including natural markers (growth factors) and tissue and cellular components (mesenchymal stem cells). (bvsalud.org)
Harvested from adipose tissue1
- Stem cells are easily harvested from adipose tissue through a less painful and minimally invasive technique with minimal risk to the patient. (ypo.education)
ASCs12
- In an effort to reduce the negative effects of inflammatory cytokines on tendon fibroblasts, adipose-derived mesenchymal stem cells: ASCs) were incorporated into the two in vitro models and their ability to modulate inflammation was investigated. (wustl.edu)
- Human Adipose-derived Stem/Stromal Cells (ASCs) have been widely used in stem cell and obesity research, as well as clinical applications including cell-based therapies, tissue engineering and reconstruction. (edu.au)
- This ease of availability and their ability to undergo osteogenic differentiation makes ASCs promising candidates for cell-based therapies for bone fractures. (upmbiomedicals.com)
- To elucidate the osteoinductive potential of a combination of these biophysical cues on ASCs, cells were embedded within anionic nanofibrillar cellulose (aNFC) hydrogels and exposed to electrical stimulation (ES) for up to 21 days. (upmbiomedicals.com)
- Taken together, our data suggest that a combination of ES with 3D cell culture can increase the osteogenic potential of ASCs. (upmbiomedicals.com)
- Adipose-derived mesenchymal stromal cells (ASCs) are multipotent stem cells which can differentiate into various cell types, including osteocytes and adipocytes. (agu.edu.tr)
- In this study, we used 2-photon live Ca2+ imaging to assess the fraction of cells showing spontaneous oscillations and the frequency of the oscillation (measured as interpeak interval-IPI) in ASCs undergoing osteogenic or adipogenic differentiation, using undifferentiated ASCs as controls. (agu.edu.tr)
- We observed that both time and differentiation treatment are important factors associated with a reduced fraction of cells showing Ca2+ oscillations, paralleled by increased IPI times, in comparison with untreated ASCs. (agu.edu.tr)
- A specific population of adipose-derived stem cells (ASCs) is expected to be an alternate source of bone marrow-derived mesenchymal stem cells (BMSCs). (jarde.jp)
- These findings suggested that overex-pression of the Cbfa1 gene induced a remarkable and rapid differentiation of ASCs into osteogenic cells. (jarde.jp)
- In vitro and in vivo results implied that Cbfa1-overexpressing ASCs may be an alternative cell source for BMSCs for bone tissue engineering. (jarde.jp)
- Group II: CAL group received autologous fat mixed with adipose derived stem cells (ASCs). (magnusconferences.com)
Autologous1
- However, the availability of viable, autologous bone grafts is limited making alternative cell-based strategies a promising therapeutic alternative. (upmbiomedicals.com)
Differentiation10
- Both adipogenic and osteogenic differentiation resulted in a reduction in Ca2+ dynamics, such as the fraction of cells showing intracellular Ca2+ oscillations and their frequency. (agu.edu.tr)
- Adipogenic differentiation was associated with a more pronounced reduction of Ca2+ dynamics compared to cells differentiating towards the osteogenic fate. (agu.edu.tr)
- These data suggest that adipogenic and osteogenic differentiation cell fates are associated with specific changes in spontaneous Ca2+ dynamics over time. (agu.edu.tr)
- While this observation is interesting and provides useful information to understand the functional correlates of stem cell differentiation, further studies are required to clarify the molecular and mechanistic correlates of these changes. (agu.edu.tr)
- Cell attachment, proliferation, and osteoblastic differentiation (RT-qPCR and alkaline phosphatase activity) were measured in human adipose-derived stem cells grown on the mantle surface. (port.ac.uk)
- To evaluate the effect of Exendine-4 (EX-4), a Glucagon-like peptide 1 (GLP-1) receptor agonist, on the differentiation of insulin-secreting cells (IPCs) from rat adipose-derived mesenchymal stem cells(ADMSCs). (celljournal.org)
- Expression of Pdx-1, Glut-2 and Insulin genes in EX-4 treated cells was significantly higher than the cells exposed to differentiation media without EX-4. (celljournal.org)
- Here, for the first time, we characterize the protein complexes associated with DDX6 in human adipose tissue-derived stem cells (hASCs) and analyze the dynamics of this helicase under different conditions of translational activity and differentiation. (fiocruz.br)
- 13 verified that stromal cells (CD 34+) derived from the adipose tissue has the potential of osteogenic differentiation both in vitro and in vivo, being considered as a promising cellular therapy for the healing of bone defects. (bvsalud.org)
- A fraction of ADIPOSE TISSUE prepared to enrich in STEM CELLS with the capacity for multi-lineage differentiation. (bvsalud.org)
Osteogenic3
- In this study, we aimed at generating osteogenic and vasculogenic constructs starting from the stromal vascular fraction (SVF) of human adipose tissue as a single cell source. (nih.gov)
- In conclusion, direct perfusion of human adipose-derived cells through ceramic scaffolds establishes a 3D culture system for osteoprogenitor and endothelial cells and generates osteogenic-vasculogenic constructs. (nih.gov)
- Although there was no significant variation in the protein composition of these complexes during early adipogenic or osteogenic induction, there was a change in the distribution pattern of DDX6: the number of DDX6 granules per cell was reduced during adipogenesis and was enhanced during osteogenesis. (fiocruz.br)
Stromal vascular fraction cells1
- After stromal vascular fractions were isolated, a mean of 4.04 × 10 6 stem cells (9.7 % of 4.16 × 10 7 stromal vascular fraction cells) were prepared and injected in the selected knees of patients after arthroscopic lavage. (thestemcellfoundation.com)
Subcutaneous adipose2
- Subcutaneous adipose tissue was harvested from both buttocks by liposuction. (thestemcellfoundation.com)
- The adult unilocular subcutaneous adipose cells have the potential in vitro to redifferentiate in osteoblast (transdifferentiation). (bvsalud.org)
Canine adipose2
- For this purpose, mesenchymal stem cells were isolated from canine adipose tissue and bee venom samples were collected from Apis mellifera anatoliaca in Muğla province of Türkiye. (trdizin.gov.tr)
- The canine adipose cells obtained not exceeding the second passage was used for topical administration with appropriate substrate on the recipient wound bed. (ijcmas.com)
Vascular7
- Adipose-derived stem cell-conditioned medium is rich in growth factors such as vascular endothelial growth factor, hepatocyte growth factor, platelet-derived growth factor, and insulin-like growth factor 1. (hairlosscure2020.com)
- Recently, the adipose tissue-derived stromal vascular fraction has become the most popular source for obtaining mesenchymal stem cells because it is less expensive and is easier to perform than bone marrow concentrate harvesting. (balkanmedicaljournal.org)
- However, no study has investigated the factors affecting the mesenchymal stem cell population in adipose tissue derived stromal vascular fraction. (balkanmedicaljournal.org)
- Understanding the interaction of patient factors with the mesenchymal stem cell count and cell viability in adipose tissue-derived stromal vascular fraction could provide crucial information for surgeons to improve patient selection and outcomes. (balkanmedicaljournal.org)
- To evaluate the factors affecting the mesenchymal stem cell count, total cell count, and cell viability in adipose tissue-derived stromal vascular fraction. (balkanmedicaljournal.org)
- This study retrospectively reviewed the medical records of 30 patients who underwent liposuction to harvest adipose tissue-derived stromal vascular fraction at our stem cell center. (balkanmedicaljournal.org)
- We recorded the mesenchymal stem cell population, cell viability, and cell count of stromal vascular fraction, and we analyzed the results to determine statistical significance. (balkanmedicaljournal.org)
Osteogenesis1
- Osteogenesis in transplants of bone marrow cells. (trdizin.gov.tr)
Osteoarthritis3
- Stem cell injections are simple and easy, so osteoarthritis patients no longer need to worry about risky surgeries or lengthy hospital visits. (cellbank.co.jp)
- Human adipose-derived mesenchymal stem cells (haMSCs) have shown efficacy in treating osteoarthritis (OA) both preclinically and clinically via intra-articular (IA) injection. (biomedcentral.com)
- In the present study, the clinical outcomes and second-look arthroscopic findings of intra-articular injection of stem cells with arthroscopic lavage for treatment of elderly patients with knee osteoarthritis (OA) were evaluated. (thestemcellfoundation.com)
Adult5
- Adult stem cells that are derived from living adults. (hairlosscure2020.com)
- Adult stem cells are found in a few select locations in the body, known as niches, such as those in the bone marrow or gonads. (wikipedia.org)
- A comparative study between the possible protective role of melatonin versus its combination with adipose derived-mesenchymal stem cells on experimentally induced diabetic retinopathy in adult male albino rats (Histological and immunohistochemical study). (qxmd.com)
- 50 adult male rats were equally divided into four groups control, diabetic, melatonin, and melatonin plus stem cells. (qxmd.com)
- bioceramics and bioglasses, raising great interest in the research with adipose tissue as a reservoir of adult stem cells, generating good results. (bvsalud.org)
Regenerative3
- However, most donor stem cells die before their regenerative effects appear. (unair.ac.id)
- Hypoxia preconditioning represents a comprehensive increase in regenerative gene expression in stem cell therapy. (unair.ac.id)
- Stem cell therapy is a form of regenerative medicine that utilizes the body's natural healing ability to repair and regenerate damaged or diseased cells, tissues and organs. (ypo.education)
Vitro and in vivo1
- Detection thresholds of the labeled cells in vitro and in vivo were determined to be 10 4 and 10 5 cells, respectively. (biomedcentral.com)
Proliferation5
- We designed a study to find theoretical evidence for the induction, movement, fusion, proliferation, and safety of human adipose mesenchymal stem cells (hADSCs) in intraocular application. (molvis.org)
- The upregulation of miR‑145 and miR‑221 was associated with the enhanced proliferation of periosteal cells and chondrogenic potential, respectively. (spandidos-publications.com)
- Results showed that the ADSC-CM increased the proliferation of the dermal papilla cells (DPCs) and the HaCaT cells. (dermatologytimes.com)
- The objective of this study is to determine the effects of bee venom on the proliferation capacity of mesenchymal stem cells and wound healing. (trdizin.gov.tr)
- According to the results of our study's cell proliferation assay and wound healing test, bee venom had no proliferative effect on mesenchymal stem cells within the defined dose range. (trdizin.gov.tr)
Tissues2
- Stem cells send signals to surrounding tissues to make new cells. (cellbank.co.jp)
- Cells Tissues Organs. (cancercentrum.se)
Cryopreservation2
- Given this, the ALINE MANICA2 cryopreservation of adipose tissue-derived mesenchymal stem cells (ADMSCs) could be a JULIANA CRISTINA SCHMIDT2 way to collect them in one surgical procedure and after performing fractional fat grafting. (bvsalud.org)
- Cryopreservation of mesenchymal stem cells: Alternative to fat grafting in breast reconstruction análise das CTDAs, após 1 e 3 meses de congelamento, indicou que as amostras tratadas com L-prolina + trealose apresentaram viabilidade semelhante àquelas preservadas com DMSO e SFB ( p =0,444). (bvsalud.org)
Hypoxia2
- There are significant advantages in stem cell therapy with hypoxia preconditioning. (unair.ac.id)
- We evaluated the effect of DEX-MVs on NRK-52E cells migration, hypoxia/reoxygenation (H/R)-induced cell death, and reactive oxygen species (ROS) amount and renal IR model in rats. (ntnu.edu.tw)
Differentiate into various2
- These super building blocks of life can differentiate into various other specialized cells or into yet other identical stem cells. (hairlosscure2020.com)
- In multicellular organisms, stem cells are undifferentiated or partially differentiated cells that can differentiate into various types of cells and proliferate indefinitely to produce more of the same stem cell. (wikipedia.org)
Scaffolds2
- SVF cells from human lipoaspirates were seeded and cultured for 5 days in porous hydroxyapatite scaffolds by alternate perfusion through the scaffold pores, eliminating standard monolayer (two-dimensional [2D]) culture. (nih.gov)
- SVF cells were also expanded in 2D culture for 5 days and statically loaded in the scaffolds. (nih.gov)
Progenitor4
- Understanding how cancer stem cells affect tumor heterogeneity, the contribution of resident stem and progenitor cells to the tumor niche, and the search for true cancer stem cell markers are other examples of scientific challenges in this fast-growing research area. (hindawi.com)
- They are usually distinguished from progenitor cells, which cannot divide indefinitely, and precursor or blast cells, which are usually committed to differentiating into one cell type. (wikipedia.org)
- they are vastly outnumbered by the progenitor cells and terminally differentiated cells that they differentiate into. (wikipedia.org)
- The stem cells coming from the adipose tissue can be an important source of progenitor mesenchymal stem cells which can be employed in tissue engineering for bone repairing 8 . (bvsalud.org)
Injections4
- Adipose injections often come with a risk of lumps forming in new tissue. (cellbank.co.jp)
- Adipose injections can help visibly reduce signs of aging by filling in wrinkles and giving skin new elasticity. (cellbank.co.jp)
- Cosmetic results from adipose-derived stem cell treatment are long-lasting and effective, offering new anti-aging alternatives to inconsistent hyaluronic acid injections. (cellbank.co.jp)
- Stem cell injections combined with arthroscopic lavage were administered to 30 elderly patients (≥65 years) with knee OA. (thestemcellfoundation.com)
Exosomes2
- Previously, we showed that exosomes derived from human adipose tissue-derived mesenchymal stem cells (ASC-exosomes) attenuate AD-like symptoms by reducing multiple inflammatory cytokine levels. (mdpi.com)
- Periosteal cells treated with exosomes exhibited higher levels of microRNA (miR)‑145 and miR‑221. (spandidos-publications.com)
Bone tissue3
- The resulting cell-scaffold constructs were either enzymatically treated to extract and characterize the cells or subcutaneously implanted in nude mice for 8 weeks to assess the capacity to form bone tissue and blood vessels. (nih.gov)
- Importantly, constructs generated under 3D perfusion, and not those based on 2D-expanded cells, reproducibly formed bone tissue. (nih.gov)
- Mesenchymal stem cells have the ability to differentiate in osteoblasts with potential implications for the engineering of human bone tissue 11 . (bvsalud.org)
ADSC1
- In a cell cycle analysis, ADSC-CM shortened G1 arrest and prolonged the synthesis and mitosis phases in the dermal papilla cells. (dermatologytimes.com)
ADMSCs2
- The percentage of insulin positive cells in the EX-4 treated group was ap- proximately 4-fold higher than in the EX-4 untreated ADMSCs. (celljournal.org)
- Dimethyl sulfoxide (DMSO) is a cryopreservative used in cell research, but it is potentially toxic, which would make it impossible to use cryopreserved ADMSCs in clinical practice. (bvsalud.org)
Viability5
- Cell viability test was performed on mesenchymal stem cells exposed to various concentrations (40 ppm, 20 ppm, 10 ppm, 5 ppm, 2.5 ppm, 1.25 ppm, 0.625 ppm and 0.312 ppm) of bee venom. (trdizin.gov.tr)
- The relationship of patient factors (age, body mass index, hypertension, and coronary artery disease) with cell viability and mesenchymal stem cell counts may be important for clinical applications. (balkanmedicaljournal.org)
- Cell viability was determined by MTT assay. (confex.com)
- The viability of hADSCs and A549 cells were not affected by the treatment of LPS and P25 TiO2. (confex.com)
- After 30 and 90 days, the samples were thawed, and cell viability was assessed using the MTT technique. (bvsalud.org)
Lumps2
- These lumps are a result of engraftment failure, but stem cells have a high engraftment rate so there's almost no risk of lumps with adipose-derived stem cells. (cellbank.co.jp)
- They observed lumps in the spleens of the mice that were linearly proportional to the number of bone marrow cells injected. (wikipedia.org)
Inflammatory5
- Adipose tissue-derived mesenchymal stem cells (AD-MSC) show great potential to treat inflammatory and degradative processes in OA and have demonstrated paracrine effects in chondrocytes. (karger.com)
- To examine the effect of inflammatory cytokines on tendon fibroblasts, the native cell type that must eventually re-build the injured tendon, we established two in vitro models of inflammation: one induced by exogenous IL-1β and one induced by macrophages: the primary cellular source of IL-1β). (wustl.edu)
- This study shows the different inflammatory responses of hADSCs and A549 cells to different stimulating materials. (confex.com)
- The response of hADSCs to inflammatory agents are more dynamics than A549 cells, which suggests that hADSC may facilitate some manipulation in certain inflammatory situation, such as chronic airway disease. (confex.com)
- Severe inflammatory infiltration with giant cells formation and fibrosis were detected Significant decrease of VEGF reaction was seen. (magnusconferences.com)
Potential4
- However, it has been shown that adipose-derived stem cells (ASC) have similar immunophenotype, morphology, multilineage potential, and transcriptome compared to BMSC. (oregonstate.edu)
- Summary Technical and ethical issues surrounding stem cells' self-expansion potential and genetic makeup exist. (psu.edu)
- The interplay between stem cell and tumor biology offers an exceptional opportunity to advance our knowledge about cancer, one of the leading causes of death worldwide, with potential impacts in cancer diagnosis and therapy. (hindawi.com)
- Therefore, these cells have great potential and can be used in the clinical treatment of PNI. (tokushima-u.ac.jp)
Cytokines1
- Human adipose tissue-derived stem cells(hADSCs) have various influences on many types of cells through production and secretion of several cytokines. (confex.com)
Embryonic6
- Embryonic stem cells that are derived from human embryos. (hairlosscure2020.com)
- In mammals, roughly 50-150 cells make up the inner cell mass during the blastocyst stage of embryonic development, around days 5-14. (wikipedia.org)
- However, when they are isolated and cultured in vitro, they can be kept in the stem-cell stage and are known as embryonic stem cells (ESCs). (wikipedia.org)
- Since 1998 however, it has been possible to culture and differentiate human embryonic stem cells (in stem-cell lines). (wikipedia.org)
- Somatic cell nuclear transfer is a cloning method that can be used to create a cloned embryo for the use of its embryonic stem cells in stem cell therapy. (wikipedia.org)
- In 1981, embryonic stem (ES) cells were first isolated and successfully cultured using mouse blastocysts by British biologists Martin Evans and Matthew Kaufman. (wikipedia.org)
Cartilage2
- Adipose-derived stem cell therapy for elderly patients with knee OA was effective in cartilage healing, reducing pain, and improving function. (thestemcellfoundation.com)
- Trang chủ Công trình KHCN Sách/Chương sách Expanded Adipose Tissue-Derived Stem Cells for Articular Cartilage Injury Treatment: A Safety. (vinastemcelllab.com)
Injection4
- The intracavernous (i.c.) injection of stem cells (SCs) has been shown to improve erectile function in various erectile dysfunction (ED) animal models. (stemcellclinicsofamerica.net)
- Another group of rats received i.c. injection of EdU-labeled allogeneic penile smooth muscle cells (PSMCs). (stemcellclinicsofamerica.net)
- So, enriching the transplanted fat with stem cells (cell assisted lipotransfer) or oral administration of alpha-lipoic acid (ALA) before and after graft injection, were thought to increase the survival rate of the transplanted fat. (magnusconferences.com)
- Following the injection of stem cells, samples of rat retina were collected for light and electron microscopy analyses. (qxmd.com)
Clinical3
- Mesenchymal stem cells for chronic wound healing: current status of preclinical and clinical studies. (trdizin.gov.tr)
- Of all the cells, mesenchymal stem cells have been and still are of interest to the researchers for use in pre-clinical and clinical studies due to being easily accessible, pluripotency, secretion of numerous growth factors, low risk of immunological rejection, and not having ethical issues for clinical use. (ac.ir)
- Stem Cells (ADSC's) for clinical research and deployment. (stemcellclinicsofamerica.net)
Inflammation2
- Temporal changes in immune cell population and gene expression of inflammation-, matrix degradation-, and extracellular matrix-related factors were examined. (wustl.edu)
- Adipose-derived stem cells can reduce inflammation and help patients manage chronic knee pain. (cellbank.co.jp)
Abdominal1
- AD-MSC were isolated by collagenase treatment from adipose tissue from healthy individuals subjected to abdominal lipectomy surgery. (karger.com)