• The aim of this study was to investigate the effects of SCFAs on adiponectin and leptin expression in adipocytes, and also to determine whether the effects of SCFA treatment in visceral adipocytes obtained from healthy subjects are different relative to the effects in adipocytes from patients with type 2 diabetes. (acta-endo.ro)
  • SCFAs inhibited lipid droplet accumulation and increased mRNA expression of adiponectin and leptin in T2D-derived adipocytes. (acta-endo.ro)
  • The nature of dietary fat determines the extent of metabolic alterations reflected in adipocytes through modifications in the pattern of adipokines secretion and modulation of key genes mRNA expression. (biomedcentral.com)
  • 2.3 Western Blots for SRF and Adiponectin 3 F442A adipocytes or mouse adipocytes were incubated in DMEM supplemented with 1% FBS made up of conjugated fatty acid for 16?h. (bios-mep.info)
  • 05. 3 Outcomes 3.1 Aftereffect of DHA on SRF and Adiponectin Amounts in 3T3-F442A Adipocytes Our prior studies show that polyunsaturated fatty acids DHA and EPA increased adiponectin PF-04217903 synthesis and secretion via PPAR (bios-mep.info)
  • Adipocytes were treated as specified control … We examined SRF and adiponectin mRNA in main cultures of mouse adipocytes treated with DHA DHA+ GW9662 or GW9662 alone for 16?h. (bios-mep.info)
  • The mRNA levels of adiponectin in mature adipocytes were examined using real-time RT-PCR. (ewha.ac.kr)
  • In 3T3-L1 adipocytes, adiponectin secretion was inhibited by 50 mmol/L glucose, PDS diluted 2-fold, and H 2 O 2 (200 μmol/L). In addition, H 2 O 2 downregulated expression of adiponectin mRNA and secretion of adiponectin oligomer complexes. (ewha.ac.kr)
  • Conclusions: Our data suggest that ROS induced by con-ventional glucose-based PDS may contribute to pathophysi-ological changes in abdominal fat and downregulate adiponectin secreted from adipocytes during long-term PD. (ewha.ac.kr)
  • Ostarine downregulates the expression of leptin and adiponectin mRNAs, as well as decreases their release from rat adipocytes. (homatics.co.kr)
  • H PPAR activation in adipocytes may well underlie its pharmacological functions, as adiponectin contributing to insulin-sensitizing and antiatherogenic effects is nicely established [8]. (lipophilic-platinum.com)
  • The adiponectin receptors, AdipoR1 and AdipoR2, are thought to transmit the insulin-sensitizing, anti-inflammatory, and atheroprotective effects of adiponectin. (diabetesjournals.org)
  • Adiponectin binds to a number of receptors. (wikipedia.org)
  • So far, two receptors have been identified with homology to G protein-coupled receptors, and one receptor similar to the cadherin family: Adiponectin receptor 1 (AdipoR1) Adiponectin receptor 2 (AdipoR2) T-cadherin - CDH13 These have distinct tissue specificities within the body and have different affinities to the various forms of adiponectin. (wikipedia.org)
  • Peroxisome Proliferator-Activated Receptors gamma-2 (PPARγ2) and estrogen receptor alpha (ERα) mRNA expression levels in adipose tissue were decreased up to 70% in HFD-fed mice but were more markedly reduced in male mice as compared with female mice. (biomedcentral.com)
  • Expression of Adiponectin and Adiponectin receptors. (aging-us.com)
  • mRNA levels of Adiponectin in WHT ( A ). Immunoblot results ( B ) and protein expression of Adiponectin ( C ). mRNA expression of the Adiponectin receptors AdipoR1 ( D ), AdipoR2 ( E ) and T-cadherin ( F ). The transcripts were analyzed by qPCR. (aging-us.com)
  • Adiponectin (APN) is the most abundant adipocytokine primarily secreted by adipose tissues and regulates various physiological processes via its receptors (AdipoR1, AdipoR2, and T-cadherin) [ 16 ]. (biomedcentral.com)
  • There are three known receptors for adiponectin: adiponectin receptor 1 (AdipoR1), adiponectin receptor 2 (AdipoR2), and T-cadherin. (encyclopedia.pub)
  • Under normal circumstances adiponectin presents anti-inflammatory, pro-apoptotic and anti-proliferative effects (Booth et al . (scielo.br)
  • Low plasma levels of the anti-inflammatory factor adiponectin characterize obesity and insulin resistance. (diabetesjournals.org)
  • The mediators of the specific weight effect on the kidney appear to be an obesity-related inflammation, aggravated by a lowered anti-inflammatory adiponectin level, an increased oxidative stress and advanced glycation, and an enhanced TGF-beta expression, all of which might constitute promising therapeutic targets. (hindawi.com)
  • The last few years have brought a rising body of evidence that adiponectin is a multipotential protein with anti-inflammatory, metabolic, anti-atherogenic, and reactive oxygen species (ROS) protective actions. (encyclopedia.pub)
  • In addition, (-)-catechin upregulated the secretion of adiponectin in hBM-MSC culture. (nih.gov)
  • Myotubes from 40 metabolically characterized donors expressed 1.8-fold more AdipoR1 than AdipoR2 mRNA (588 ± 35 vs. 321 ± 39 fg/μg total RNA). (diabetesjournals.org)
  • AdipoR1 mRNA expression was positively correlated with in vivo insulin and C-peptide concentrations, first-phase insulin secretion, and plasma triglyceride and cholesterol concentrations before and after adjustment for sex, age, waist-to-hip ratio, and body fat. (diabetesjournals.org)
  • In multivariate linear regression models, mRNA expression of AdipoR1, but not AdipoR2, was a determinant of first-phase insulin secretion independent of insulin sensitivity and body fat. (diabetesjournals.org)
  • Globular adiponectin has the highest affinity to AdipoR1, therefore in animal studies it acts mainly in muscle cells [ 1 ] . (encyclopedia.pub)
  • In view of the absence of reports regarding adiponectin levels in dogs and the occurrence of malignant neoplasms, the present study aimed to compare the serum levels of adiponectin in clinically normal bitches and in those with a diagnosis of mammary carcinoma. (scielo.br)
  • Adiponectin (also referred to as GBP-28, apM1, AdipoQ and Acrp30) is a protein hormone and adipokine, which is involved in regulating glucose levels and fatty acid breakdown. (wikipedia.org)
  • There are several factors regulating the expression of adiponectin gene ( AdipoQ ), such as: forkhead box O1 (FOXO1), peroxisome proliferator-activated receptor γ (PPAR-γ), CCAAAT-enhancer binding protein α, and sterol regulatory element binding protein 1c (SREBP-1c) [ 9 ] . (encyclopedia.pub)
  • Adiponectin, an adipokine commonly regarded as a negative indicator of metabolic disease, is reported to be downregulated in its gene level in end-stage renal disease patients. (ewha.ac.kr)
  • Adiponectin is the adipokine associated with insulin sensitization, reducing liver gluconeogenesis, and increasing fatty acid oxidation and glucose uptake. (encyclopedia.pub)
  • TAN, P.H. Evolving role of adiponectin in cancer controversies and update. (scielo.br)
  • MANTZOROS, C.S. The role of adiponectin in cancer: a review of current evidence. (scielo.br)
  • Conclusion: These new mechanistic and pathophysiology studies provide evidence for an important role of adiponectin in colon cancer. (nuthealth.org)
  • Salutary effects of adiponectin on colon cancer: in vivo and in vitro studies in mice. (nuthealth.org)
  • No previous studies have directly investigated the mechanistic effects of adiponectin on colon cancer in the settings of obesity, a high-fat diet and/or adiponectin deficiency. (nuthealth.org)
  • Objective: To investigate the effects of adiponectin on the growth of colorectal cancer in adiponectin-deficient or wild-type-C57BL/6 mice fed a low-fat or high-fat diet. (nuthealth.org)
  • Expression of AdipoR2 mRNA clearly associated only with plasma triglyceride concentrations. (diabetesjournals.org)
  • Circulating adiponectin concentrations increase during caloric restriction in animals and humans, such as in patients with anorexia nervosa. (wikipedia.org)
  • Treatment with increasing concentrations of simvastatin lowered leptin mRNA (A) and leptin secretion (B). secretion, cells had been incubated with PD98059 (30?via T0070907 avoided statin\mediated lowers in leptin secretion (simvastatin: reduced leptin secretion towards the same level as that induced by atorvastatin and simvastatin. (monossabios.com)
  • Treatment with raising concentrations of simvastatin reduced leptin mRNA (A) and leptin secretion (B). Treatment with raising concentrations of atorvastatin reduced leptin mRNA (C) and leptin secretion (D). Data are shown as mean? (monossabios.com)
  • We used increasing concentrations of atorvastatin and in addition examined the consequences of statins in leptin leptin and mRNA secretion. (monossabios.com)
  • Adiponectin concentrations are gender specific, with higher levels in females [ 2 ] [ 3 ] . (encyclopedia.pub)
  • The function of thiazolidinediones may well increase insulin sensitivity by growing concentrations of adiponectin and by decreasing cost-free fatty acid and inflammatory factor TNF- levels in diabetic subjects and Caspase 6 medchemexpress animal models [12, 13]. (lipophilic-platinum.com)
  • However, coronary artery disease has been found to be positively associated with high molecular weight adiponectin, but not with low molecular weight adiponectin. (wikipedia.org)
  • Adipocyte size, both in epicardial and in subcutaneous fat, is positively related with insulin resistance, shows negative association with local adiponectin gene expression, and is decreased in subjects with coronary artery disease. (univr.it)
  • To elucidate the relationship between plasma levels of adiponectin, adiponectin gene expression in adipose tissue, and markers of inflammation, we obtained blood samples, anthropometric measures, and subcutaneous adipose tissue samples from 65 postmenopausal healthy women. (diabetesjournals.org)
  • The fasting blood glucose and serum insulin were measured, the insulin action index (IAI) was calculated, the serum adiponectin was determined by ELISA, and the expression of adiponectin mRNA in abdominal subcutaneous adipose tissues and periepididymal adipose tissues was detected by quantitative Real-Time PCR. (shsmu.edu.cn)
  • However, rosiglitazone significantly increased adiponectin protein expression in WAT, suggesting that WAT contributes to hyperadiponectinemia in this context. (frontiersin.org)
  • Adiponectin protein expression was obtained by Western blot analysis and quantified with Image Lab 6.1 software. (aging-us.com)
  • Our benefits showed that TG and 2TG increased adiponectin mRNA and protein expression and that this impact was mediated by AMPK phosphorylation. (lipophilic-platinum.com)
  • Transgenic mice with increased adiponectin show reduced adipocyte differentiation and increased energy expenditure associated with mitochondrial uncoupling. (wikipedia.org)
  • Catechin increased the mRNA levels of various adipogenic markers, such as adiponectin, peroxisome proliferator-activated receptor gamma (PPARgamma), FABP4, and LPL, as measured during adipocyte differentiation in hBM-MSCs. (nih.gov)
  • It is also related to increased adiponectin (APN) expression associated with adipocyte differentiation, as well as the expression of adipogenic genes such as peroxisome proliferator-activated receptor ( PPAR) ( 22 , 23 ). (frontiersin.org)
  • High adiponectin levels correlate with a lower risk of diabetes mellitus type 2. (wikipedia.org)
  • Plasma levels of adiponectin are lower in obese subjects than in lean subjects. (wikipedia.org)
  • Humans with lower levels of adiponectin have reduced cognitive function. (wikipedia.org)
  • Adiponectin plasma levels and adipose-tissue gene expression were significantly lower in obese subjects and inversely correlated with obesity-associated variables, including high-sensitive C-reactive protein (hs-CRP) and interleukin-6 (IL-6). (diabetesjournals.org)
  • 0.05), whereas the inverse correlation between adiponectin plasma levels or adiponectin gene expression in adipose tissue with plasma IL-6 were largely dependent on the clustering of obesity-associated variables. (diabetesjournals.org)
  • In conclusion, our data suggest a transcriptional mechanism leading to decreased adiponectin plasma levels in obese women and demonstrate that low levels of adiponectin are associated with higher levels of hs-CRP and IL-6, two inflammatory mediators and markers of increased cardiovascular risk. (diabetesjournals.org)
  • These studies suggest that InnoSlim® could achieve this by activating a specific pathway called the adiponectin and AMPK signaling pathway, which plays a role in regulating the levels of fats and sugar in the bloodstream. (nulivscience.com)
  • Decreased levels of adiponectin are linked to difficulties in regulating blood sugar and breaking down fatty acids. (nulivscience.com)
  • When adiponectin levels increase, there is a subsequent rise in AMPK phosphorylation, which promotes the breakdown of fatty acids. (nulivscience.com)
  • By increasing adiponectin levels and secretion in fat cells, InnoSlim® works to stimulate the phosphorylation and activation of AMPK in skeletal muscles. (nulivscience.com)
  • 1, 2, 3 Moreover, human clinical trials have further supported these findings by demonstrating that InnoSlim® may enhance the adiponectin and AMPK signaling pathway in tested subjects, leading to potential improvements in blood sugar and lipid levels. (nulivscience.com)
  • In addition, mRNA levels of adiponectin, leptin and Peroxisome Proliferator-Activated Receptor γ (PPARγ) were determined by Real-Time PCR system. (acta-endo.ro)
  • Low adiponectin levels may play an important role in the development of colon and other obesity-related malignancies. (nuthealth.org)
  • Adiponectin treatment decreased serum insulin levels in mice on a high-fat-diet and increased serum-interleukin (IL)-12 levels in adiponectin-deficient mice. (nuthealth.org)
  • Body weight control thus impacts on local intrarenal advanced glycation and oxidative stress through inflammation and adiponectin levels. (hindawi.com)
  • Body weight, plasmatic parameters and adipose tissue mRNA expression levels of key genes were evaluated after 20 weeks of HFD feeding. (biomedcentral.com)
  • Although its plasma levels remained unchanged in females, adiponectin mRNA levels were significantly reduced in adipose tissue of both HFD-fed groups with a more marked decrease in AD group compared to VD group. (biomedcentral.com)
  • Adiponectin mRNA levels were determined by real-time polymerase chain reaction on subcutaneous fat and epicardial fat biopsies. (univr.it)
  • Adiponectin levels in the conditioned media were measured by ELISA and immunoblot assays. (ewha.ac.kr)
  • Results: The levels of adiponectin in plasma and spent dialysate were significantly downregulated by PDS and this effect was suppressed by NAC. (ewha.ac.kr)
  • Conclusions: Given the decreased expression levels of sestrin 2, adiponectin, and AMPK genes as tumor inhibitors and the increased expression level of the mTOR gene as an oncogene in the patients with AML in our study, it is thought that disruption of this pathway may be involved in leukemogenesis and can be considered as an effective factor in the progression of cancer. (ijbiotech.com)
  • After 8 weeks of combined station training, the levels of adiponectin in experimental group increased significantly ( P =0.001) while the levels of hs-CRP decreased ( P =0.001). (ac.ir)
  • Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. (wikipedia.org)
  • In vitro, it was found that adiponectin directly controls malignant potential (cell proliferation, adhesion, invasion and colony formation) and regulates metabolic (AMPK/S6), inflammatory (STAT3/VEGF) and cell cycle (p21/p27/p53/cyclins) signalling pathways in both mouse MCA38 and human HT29, HCT116 and LoVo colon cancer cell lines in a LKB1-dependent way. (nuthealth.org)
  • As we expected, MTMR14 mRNA and protein were almost undetectable in the metabolic tissues of male KO mice at different ages (Fig. 1 b, c). (biomedcentral.com)
  • Adiponectin is secreted from adipose tissue (and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones. (wikipedia.org)
  • Adiponectin is secreted into the bloodstream, where it accounts for about 0.01% of all plasma protein at around 5-10 μg/mL. (wikipedia.org)
  • 10 mmol/L). ELISA was used to quantify adiponectin in plasma and spent dialysate. (ewha.ac.kr)
  • Rosiglitazone elevated plasma adiponectin level and normalized peroxisomal fatty acid β-oxidation rate. (cancerhugs.com)
  • Ethanol publicity reduced plasma FFA and cholesterol amounts but didn't have an effect on various other variables including blood sugar insulin and adiponectin. (cancerhugs.com)
  • Rosiglitazone attenuated ethanol's influence on ALT plasma cholesterol and ketone systems and elevated plasma adiponectin level by threefold. (cancerhugs.com)
  • Adiponectin functions by controlling the cellular energy metabolism through the regulation of the AMPK signaling pathway. (nulivscience.com)
  • In this study, we tested our hypothesis that oxidative stress induced by PDS may play a role in the regulation of adiponectin. (ewha.ac.kr)
  • Adiponectin connects energy balance regulation in the central nervous system and peripheral tissues [ 10 ] . (encyclopedia.pub)
  • Regulation of adiponectin expression requires a complicated array of intracellular signaling pathways involving PPAR and AMPK [14, 15]. (lipophilic-platinum.com)
  • Mediators of the renoprotection, specifically linked to obesity and body weight control, include a reduced renal inflammation and TGF-beta expression, together with an enhanced level of adiponectin. (hindawi.com)
  • Adiponectin in combination with leptin has been shown to completely reverse insulin resistance in mice. (wikipedia.org)
  • TZD-induced hyperadiponectinemia is closely associated with increased adiponectin production in MAT but is not prevented by the partial loss of MAT that occurs in Ocn- Wnt10b mice. (frontiersin.org)
  • To generate mice lacking TGs in adipose tissue (ADGAT DKO), we crossed adipose tissue-specific Dgat1 knockout mice (Cre-transgene expressed under control of the mouse adiponectin promoter 3 ) with Dgat2 flox mice 4 . (elifesciences.org)
  • Adiponectin treatment also suppressed angiogenesis assessed by CD31 staining and VEGFb and VEGFd mRNA expression in tumours obtained from mice fed a high-fat diet and from adiponectin-deficient mice. (nuthealth.org)
  • Objective To investigate the effect of curcumin on expression of adiponectin in mice with insulin resistance. (shsmu.edu.cn)
  • Conclusion Curcumin has definite protective effect on mice with insulin resistance, and the mechanism may be the upregulation of expression of adiponectin mRNA. (shsmu.edu.cn)
  • Adiponectin gene expression was lower in the latter than in the former, although not significantly. (univr.it)
  • Adiponectin gene expression is significantly lower in epicardial- than in subcutaneous fat. (univr.it)
  • PPAR-γ mRNA level in the adipose tissues was reduced by ethanol feeding that was normalized by rosiglitazone supplementation significantly. (cancerhugs.com)
  • Our prior study demonstrated that PPAR agonist rosiglitazone inhibits monocyte adhesion to fibronectin-coated plates via de novo adiponectin production in human monocytes [11]. (lipophilic-platinum.com)
  • The aims of this study have been to investigate the effects of TG and 2TG on the adiponectin expression in THP-1 cells and to decide no matter if PPAR and AMPK have been involved. (lipophilic-platinum.com)
  • Objective: In this study, expression of mTOR genes and three upstream targets (5' adenosine monophosphate-activated protein kinase (AMPK, adiponectin, and sestrin 2) involved in mTOR signaling was investigated. (ijbiotech.com)
  • Materials and Methods: In this study, expression of mTOR, AMPK, sestrin 2, and adiponectin genes in 60 patients with AML were evaluated compared to those of 30 healthy individuals as controls using the Real-Time polymerase chain reaction (Real-Time RT-PCR) method. (ijbiotech.com)
  • Expression of the mTOR gene was increased, while expression of AMPK, sestrin 2, and adiponectin genes was decreased in the patients with AML. (ijbiotech.com)
  • Data from large cross-sectional and cohort studies showed a positive correlation between serum adiponectin and mortality in chronic kidney disease. (encyclopedia.pub)
  • 185 genes in signature reported in the publication with PubMedID mRNA_KAT5_22196727 from the ESCAPE Omics Signatures of Genes and Proteins for Stem Cells dataset. (maayanlab.cloud)
  • Radix Astragali and Notoginseng saponins improve glucose and fatty acid metabolism through upregulated adiponectin and its downstream signaling. (nulivscience.com)
  • Interestingly, animal studies have shown that adiponectin ameliorates the proinflammatory effect of saturated free fatty acid rich diet in hypothalamus. (encyclopedia.pub)
  • In this study, we examined whether AdipoR mRNA expression in human myotubes correlates with in vivo measures of insulin sensitivity. (diabetesjournals.org)
  • Since its discovery, adiponectin has been identified as one of the key regulators involved in glucose and lipid metabolism. (encyclopedia.pub)
  • Adiponectin lowers glucose production by increasing SOGA. (nih.gov)
  • Since prior studies have recommended that ERK works through the activation of PPARpathways to modulate transcription of focus on protein (Paumelle and Staels 2007), chances are that statins activate ERK1/2 which activates PPARand thus lowers the transcription of leptin mRNA. (monossabios.com)
  • As an example, adiponectin might be one of the key mediators responsible for regular physical activity benefits in humans. (encyclopedia.pub)
  • Thus, the implication of adiponectin in the pathogenesis of cancer is well established in humans, but still undetermined in dogs. (scielo.br)
  • It is generated by proteolytic cleavage of full-length adiponectin and has biological activity in humans [ 1 ] . (encyclopedia.pub)
  • Despite more than 20 years of constant studies on adiponectin function in animals and humans, an increasing quantity of data has been added recently. (encyclopedia.pub)
  • High-molecular-weight adiponectin was further found to be associated with a lower risk of diabetes with similar magnitude of association as total adiponectin. (wikipedia.org)
  • SEM (inhibitor (T0070907) decreased the secretion of MCP1 (marginally decreased great molecular pounds adiponectin secretion (pathway didn't alter secretion of MCP1 (inhibitors didn't further alter secretion of MCP1 (PD98059 vs. PD98059+simvastatin, pathway are essential for the statin\mediated legislation of MCP1, total and high molecular pounds adiponectin. (monossabios.com)
  • Since excessive fat deposit is involved in increased reactive oxygen species (ROS), PD solution (PDS) may contribute to ROS production, resulting in dysregulation of adiponectin. (ewha.ac.kr)
  • Some studies have indicated that T-cadherin is a major binding partner for adiponectin, and causes its accumulation in the heart, vascular endothelium, and skeletal muscle. (encyclopedia.pub)