Adipocyte differentiation of 3t3. Medical search. Frequent questions

This study aimed to determine whether I3C or DIM could increase glucose uptake via enhanced insulin sensitivity in 3T3‐L1 adipocytes, as well as the mechanism involved. (researchgate.net)
DIM, a major metabolite of indole-3-carbinol, which is naturally produced in broccoli and cabbage, enhances glucose uptake through the improvement of insulin sensitivity in 3T3-L1 cells [13] . (researchgate.net)
While the physiological role of adipose tissue in cholesterol and oxLDL metabolism remains to be established, the induction of OLR1 is a potential means by which PPARγ ligands regulate lipid metabolism and insulin sensitivity in adipocytes. (jci.org)

Previous studies have found that DIM can improve type 2 diabetes by enhancing glucose uptake through the activation of insulin signaling in 3T3-L1 cells, and by lowering the plasma glucose levels in high-fat-diet-fed obese mice [13, 14]. (researchgate.net)
the effects of hypoxia and fibrosis may also play a role in obesity complications via notably ectopic-lipid accumulation in non-adipocyte cells. (springer.com)
Concentrations of cholesterol and triglycerides are strongly correlated in the adipocyte, but little is known about mechanisms regulating cholesterol metabolism in fat cells. (jci.org)
In vitro analysis with 3T3-F442A cells revealed anti-proliferative and anti-differentiation effects of OBEX. (nature.com)
In addition, OBEX induced a clear reduction of the lipid load in mature adipocytes obtained from 3T3-F442A cells. (nature.com)
Alteration by 2,3,7,8-Tetrachlorodibenzo-p-dioxin of CCAAT/enhancer binding protein correlates with suppression of adipocyte differentiation in 3T3-L1 cells. (aspetjournals.org)
Gel shift analysis of nuclear extract binding to an oligonucleotide containing a C/EBP DNA recognition sequence revealed no difference between extracts from control and TCDD-treated cells in the binding pattern at day 2 of differentiation. (aspetjournals.org)
Granéli C, Karlsson C, Brisby H, Lindahl A and Thomsen P: The effects of PPAR-γ inhibition on gene expression and the progression of induced osteogenic differentiation of human mesenchymal stem cells. (spandidos-publications.com)
The differentiation of precursor cells into mature adipocytes (adipogenesis) has been an area of increased focus, spurred by a rise in obesity rates. (oregonstate.edu)
Objective : We investigated the anti-adipogenic effects of T. majus ethanol extract (TME) on 3T3-L1 cells. (foodandnutritionresearch.net)
Design : 3T3-L1 cells were differentiated in the presence of different concentrations of TME. (foodandnutritionresearch.net)
Changes in the expression of proteins related to adipocyte differentiation in 3T3-L1 cells were determined by SDS-PAGE and western blotting. (foodandnutritionresearch.net)
Discussion : Taken together, the results of this study demonstrate that TME inhibits lipid accumulation and reduces the expression PPARG, CEBPA, and SREBF1, which regulate adipocyte differentiation in 3T3-L1 cells. (foodandnutritionresearch.net)
In the 3T3-L1 cells HuR is constitutively expressed and localized predominantly to the nucleus in the preadipocytes. (uncg.edu)
Constitutive GATA-2 and GATA-3 expression suppressed adipocyte differentiation and trapped cells at the preadipocyte stage. (nih.gov)
GATA-3-deficient embryonic stem cells exhibit an enhanced capacity to differentiate into adipocytes, and defective GATA-2 and GATA-3 expression is associated with obesity. (nih.gov)
Moreover, proliferating cell nuclear antigen (PCNA) and CD31 showed co-localization with α-SMA, suggesting the differentiation of hBMSCs into epithelial cells and myofibroblasts/fibroblasts. (ijbs.com)
In order to eliminate the interference from the transplanted cells themselves, fetal stem cells were used for transplantation because the proliferation and differentiation potentials of fetal stem cells are superior to the adult ones ( 8 ). (ijbs.com)
Additionally, we show that multiple changes in the UPP occur during the differentiation of 3T3-L1 cells into adipocytes. (elsevierpure.com)
1994). Furthermore, LOX participates in the dedication of pluripotent stem cells towards the adipocyte lineage (Huang et al. (exposed-skin-care.net)
In obese rats, the inhibition of LOX activity through -aminopropionitrile (BAPN, a particular inhibitor of LOX activity) decreases adipose cells fibrosis, partly corrects the adipocyte-size distribution design (moving it toward smaller sized sizes) and attenuates the upsurge in bodyweight and extra fat mass. (exposed-skin-care.net)

Adipogenesis continues throughout the life of adipose tissue, with continual differentiation of preadipocytes essential to maintaining tissue function during aging, and UPP alterations in mature adipocytes are likely to directly modulate adipose function during aging. (elsevierpure.com)
Taken together, these data demonstrate that changes in the UPP occur in response to adipose aging and adipogenesis and strongly suggest that proteasome inhibition is sufficient to decrease adipose differentiation, as well as increasing oxidative stress in mature adipocytes, both of which probably promote deleterious effects on adipose aging. (elsevierpure.com)

Methods 3T3‐L1 preadipocytes were differentiated by using a mixture of adipogenic inducers, including a suboptimal concentration of insulin. (researchgate.net)
Examination of the expression of several early marker genes of preadipocyte differentiation through Northern blot analysis and of differentiation-dependent mitosis showed that TCDD did not interfere with the earliest known responses of preadipocytes to inducers of differentiation. (aspetjournals.org)

through the coordinated actions of several transcription factors (TFs) In this study, we examined the dynamics of several key transcription factors (TFs) - PPARγ, C/EBPβ, CREB, NFAT, FoxO1, and SREBP-1c - during adipogenic differentiation (week 1) and ensuing lipid accumulation. (tufts.edu)
Here we report that antidiabetic thiazolidinediones (TZDs) and other ligands for the nuclear receptor PPARγ dramatically upregulate oxidized LDL receptor 1 (OLR1) in adipocytes by facilitating the exchange of coactivators for corepressors on the OLR1 gene in cultured mouse adipocytes. (jci.org)
These data identify OLR1 as a novel PPARγ target gene in adipocytes. (jci.org)
The differentiation-dependent induction of PPAR gamma mRNA was also blocked by TCDD. (aspetjournals.org)
It is also related to increased adiponectin (APN) expression associated with adipocyte differentiation, as well as the expression of adipogenic genes such as peroxisome proliferator-activated receptor ( PPAR) ( 22 , 23 ). (frontiersin.org)
The main pathogenesis of OP is excessive proliferation and differentiation of osteoclasts, in which the peroxisome proliferator‑activated receptor γ (PPARγ) pathway has a pivotal role. (spandidos-publications.com)
Regarding the underlying mechanism, inhibition of HSP90α could block the nuclear import of PPARγ to inhibit osteoclast differentiation and proliferation. (spandidos-publications.com)
2003). Selective Cellular uptake of fatty acids and following storage in the form of disruption of Pparγ2 or adipocyte-speci﫿c Pparγ knockout leads TGs in adipocytes are key steps in lipid storage. (deepdyve.com)

In the first one, local adipose tissue hypoxia could induce fibrosis accumulation which in turn through adipocyte dysfunctions might provoke adipose tissue inflammation. (springer.com)
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and related compounds elicit multiple effects on the function of adipose tissue and adipogenic cell lines, including the suppression of adipocyte differentiation. (aspetjournals.org)
Adipocytes arise from the commitment and differentiation of adipose precursors in white adipose tissue (WAT). (nih.gov)
How the UPP is altered in aging adipose tissue and adipocyte differentiation and the effects of proteasome inhibition on adipocyte homeostasis and differentiation are critical issues to elucidate experimentally. (elsevierpure.com)

Results DIM, but not I3C, increased adipocyte differentiation through upregulation of peroxisome proliferator‐activated receptor γ and CCAAT/enhancer‐binding protein α. (researchgate.net)
Additionally, TME concentrations ranging from 20 µg/mL to 500 µg/mL led to a decrease in the expression of adipocyte differentiation regulators, peroxisome proliferator-activated receptor γ, CCAAT element binding protein α, and sterol regulatory element binding transcription factor 1. (foodandnutritionresearch.net)

DIM also enhanced glucose uptake by increasing expression of glucose transporter 4 in adipocytes. (researchgate.net)
3,3′-diindolylmethane (DIM)-a natural compound produced from indole-3-carbinol, found in cruciferous vegetables-enhances glucose uptake by increasing the activation of the insulin signaling pathway in 3T3-L1 adipocytes. (researchgate.net)

MicroRNAs constitute a pervasive post-transcriptional filter on protein or mRNA expression levels that are likely to control developmental timing, cellular differentiation, stress responses, metabolism and proliferation. (mdpi.com)
In addition, risperidone influences adipocyte metabolism, differentiation, and lipid accumulation-related functions through transcriptome analysis. (frontiersin.org)
In adipocyte Senp2-de﫿ciency mice, accumulation of the SUMOylated Setdb1 suppressed the expression of Pparg and Cebpa genes as well as lipid metabolism-related target genes, which would decrease the ability of lipid storage in adipocytes. (deepdyve.com)

Firstly, the expression of HSP90α in osteoclast differentiation was detected by western blotting in vitro, then the effect of HSP90α inhibition on the formation and differentiation of osteoclasts was examined. (spandidos-publications.com)
In vitro data link observed UPP alterations to increased levels of oxidative stress and altered adipose biology relevant to both aging and differentiation. (elsevierpure.com)

The activation profiles of these TFs at different times following induction of adipogenic differentiation were quantified using 3T3-L1 reporter cell lines constructed to secrete the Gaussia luciferase enzyme upon binding of a TF to its DNA binding element. (tufts.edu)
Furthermore, we show that in maintaining early adipose precursors, Sox9 activates Meis1, which prevents adipogenic differentiation. (nih.gov)

Classically the fat mass expansion results with an increase in the volume (hypertrophy) and the number (hyperplasia) of adipocytes [ 5 , 6 ]. (springer.com)
Obesity can be viewed as a state of long-term lipid disequilibrium that is marked by massive adipocyte hypertrophy and is a major risk factor for developing insulin resistance and type 2 diabetes. (jci.org)

If the capacity of the adipocyte to store lipids is exceeded, it can no longer regulate the release of FFAs into the circulation, which ultimately leads to the abnormal accumulation of lipid in nonadipose depots. (jci.org)
Thus, GATA-2 and GATA-3 regulate adipocyte differentiation through molecular control of the preadipocyte-adipocyte transition. (nih.gov)

Here, we show that murine GATA-2 and GATA-3 are specifically expressed in white adipocyte precursors and that their down-regulation sets the stage for terminal differentiation. (nih.gov)

We began to examine the mechanism by which TCDD inhibits differentiation of the established preadipocyte cell line 3T3-L1. (aspetjournals.org)

Lipid accumulation in adipocytes reflects a balance between enzymatic pathways leading to the formation and breakdown of esterified lipids, primarily triglycerides. (tufts.edu)
Risperidone promotes adipocyte differentiation and lipid accumulation through Oil Red O staining and reverse transcription-polymerase chain reaction (RT-PCR). (frontiersin.org)
Risperidone increases adipocyte lipid accumulation by plausibly inhibiting long-chain fatty acid β-oxidation through targeting MAPK14 and MAPK8. (frontiersin.org)
Adipocyte Senp2 de﫿ciency resulted in less adipose lipid storage accompanied by an ectopic fat accumulation and insulin resistance under high-fat diet feed- ing. (deepdyve.com)
adqcKO Mechanistically, adipocyte Senp2 de﫿ciency caused the downregula- Senp2 mice exhibit an ectopic lipid accumulation and tion of Pparg and Cebpa as well as their downstream target genes insulin resistance related to lipid storage. (deepdyve.com)

Thus any modification of ECM components can be transmitted via integrins and impact on cell movement during development and proliferation, differentiation, apoptosis. (springer.com)

In addition, its action on the 3T3-F442A cell line was tested in terms of regulation of proliferation capacity and differentiation into mature adipocytes as well as its effects on mature adipocytes. (nature.com)
These data indicate that suppression of differentiation in the 3T3-L1 preadipocyte cell line by TCDD occurs at a short but defined period, during the differentiation program, and involves altered regulation of C/EBP, including the inhibition of C/EBP alpha. (aspetjournals.org)
Rayalam, S., Della-Fera, M.A. and Baile, C.A. (2008) Phytochemicals and Regulation of the Adipocyte Life Cycle. (scirp.org)

Analysis of mRNA for three isoforms of the CCAAT/enhancer binding protein (C/EBP) revealed that TCDD (5 nM) selectively inhibited the induction of C/EBP alpha mRNA but did not block the induction of C/EBP beta and C/EBP delta in response to differentiation inducers. (aspetjournals.org)

This mouse also showed an ectopic lipid in﫿ammation indicates that HFD induced adipocyte death would distribution and insulin resistance. (deepdyve.com)

irreversible inhibitor of LOX activity, attenuated the upsurge in bodyweight and extra fat mass that was seen in obese pets and shifted adipocyte size toward smaller sized adipocytes. (exposed-skin-care.net)

This balance is extremely important, as both high and low lipid levels in adipocytes can have deleterious consequences. (tufts.edu)

Finally, BAPN also normalises the insulin-stimulated blood sugar uptake and proteins degrees of GLUT4, adiponectin and SOCS3 buy 76584-70-8 in the TNF-induced insulin-resistant 3T3-L1 adipocyte model. (exposed-skin-care.net)

This was associated with DIM‐enhanced phosphorylation of the signaling intermediates Akt, insulin receptor substrate‐1, and insulin receptor early in differentiation. (researchgate.net)

Analysis of transcription factor network underlying 3T3-L1 adipocyte differentiation. (tufts.edu)

Inhibition of HSP90α could block the formation and differentiation of osteoclasts, and remit osteoporosis in mice. (spandidos-publications.com)
Although less fat stor- adqcKO pose lipid storage in adipocyte-speci﫿c Senp2 knockout mice fed age was shown in Senp2 adipose tissues, the increased with high-fat diets (HFD). (deepdyve.com)

However, the role of microRNA in mammalian tissue development and differentiation is still quite uncharacterized. (mdpi.com)

RESULTS: The deletion of BSCL2 in mature brown adipocytes increased sympathetic nervous system-independent cAMP/protein kinase A (PKA) signaling in BAT. (bvsalud.org)

Increased OLR1 expression, resulting either from TZD treatment or adenoviral gene delivery, significantly augments adipocyte cholesterol content and enhances fatty acid uptake. (jci.org)
As a result, in the process of osteoclast differentiation and proliferation, the expression of HSP90α was upregulated. (spandidos-publications.com)

TZDs markedly stimulate the uptake of oxidized LDL (oxLDL) into adipocytes, and this requires OLR1. (jci.org)

However within 30 min of exposure to the differentiation stimulus the HuR content in the cytosol increases consistent with HuR regulating the availability of relevant mRNAs for translation. (uncg.edu)

In addition, on the 3T3-F442A cell line, OBEX inhibited proliferation of pre-adipocytes, blocked the differentiation of pre-adipocytes into new adipocytes, and decreased the lipid load in mature adipocytes. (nature.com)

The present study discusses the efficacy of Aloe emodin-8-O-glycoside (AEG), a plant derived anthroquinone, on alleviating insulin resistance and augmenting glycogen synthesis in L6 myotubes and 3T3L1 adipocytes. (wiley.com)
The study showed that Bayesian network is a good method to deal with the information of symptoms and signs for syndrome differentiation, but it is also not to reflect comprehensively the thinking ability of TCM doctors in doing syndrome differentiation. (jcimjournal.com)

Whole cell extracts (30 µg lysate) of 3T3-L1 (Lane 1), 3T3-L1 differentiated to adipocytes (Lane 2), Mouse Adipose (Lane 3), Rat Adipose (Lane 4), Mouse Brown Fat (Lane 5) and Mouse Skeletal Muscle (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322B. (thermofisher.com)

Correspondence to: Jinke Cheng, E-mail: [email protected] Edited by Feng Liu One major function of adipocytes is to store excess energy in the form of triglycerides. (deepdyve.com)

Anatomy37

Organisms14

Diseases7

Chemicals and Drugs124

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes55

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science3

Insulin sensitivity3

Cells21

Adipogenesis2

Preadipocytes2

PPAR8

Adipose tissue4

Peroxisome2

Glucose uptake2

Metabolism3

Vitro2

Adipogenic differentiation2

Hypertrophy2

Regulate2

Precursors1

Inhibits1

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MRNA1

Ectopic-lipid1

Obese1

Deleterious1

Proteins1

Phosphorylation1

Transcription1

Mice2

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Expression2

Uptake1

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