• Pevonedistat (MLN4924) is a selective small molecule targeting the NEDD8-activating enzyme (NAE) and inhibits an early step in neddylation, resulting in DNA re-replication, cell cycle arrest and death. (nih.gov)
  • Global gene expression: We have determined that genes involved in the pro-inflammatory response, cell adhesion molecule regulation, apoptosis and cell cycle control are modulated during sepsis. (omicsonline.org)
  • However, multiple groups have created small molecule antagonists capable of targeting PCNA without affecting normal cells. (purdue.edu)
  • Galactosyltransferase (GalTase) is localized in the Golgi, where it functions in oligosaccharide synthesis, as well as on the cell surface where it serves as a cell adhesion molecule. (tmc.edu)
  • IHC analysis of Pax7 and neural cell adhesion molecule (NCAM) appeared to sufficiently and similarly represent the expansion of SCs after injury (28-36% increase). (mcmaster.ca)
  • DNAM-1 serves as a signaling-transducing adhesion molecule, and it physically and functionally associates with LFA-1 (CD11a/CD18) on NK and T cells. (biolegend.com)
  • Furthermore, the increased expression of PAI-1 and intercellular adhesion molecule-1 in diabetic renal cortex were also reduced by RSV administration. (hindawi.com)
  • The hormonally active form of vitamin D, 1,25-dihydroxyvitamin D (1,25D3), has shown to induce anti-cancerous effects through its cognate vitamin D receptor (VDR), in part through inducing the cell-cell adhesion molecule E-cadherin and inhibiting Wnt/β-catenin signalling. (bl.uk)
  • S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (cancerindex.org)
  • In Xenopus AGR2 plays a role in cement gland differentiation, but in human cancer cell lines high levels of AGR2 correlate with downregulation of the p53 response, cell migration, and cell transformation. (wikipedia.org)
  • In addition, their osteogenic differentiation potential was increased, and genes involved in cell adhesion, FGF-2 signalling, cell cycle, stemness, cell differentiation, and cell proliferation were upregulated, compared to that of the MSCs cultured on uncoated plates. (hindawi.com)
  • The differentiation of mesenchymal stem cells (MSCs) into multiple cell lineages can be exploited as an attractive strategy for cell-based therapy and regenerative medicine [ 1 ]. (hindawi.com)
  • The ECM provides structural and biochemical support to the cells and has various other functions including cell adhesion, cell to cell communication, and differentiation [ 17 , 18 ]. (hindawi.com)
  • Both agents concomitantly induced a series of changes in the CBS cells that influence proliferation and differentiation, but cellular responses to the two agents were not identical. (aacrjournals.org)
  • The object of this work has been to investigate the formation and function of GalTase-specific adhesions during F9 cell growth and differentiation. (tmc.edu)
  • Disruption of GalTase adhesion by exposure to anti-GalTase antibodies during this period resulted in extension of the G2 phase, a result similar to that seen with agents known to inhibit growth and induce differentiation. (tmc.edu)
  • Finally, other studies have suggested that a subset of cell adhesion molecules have the capability to induce differentiation in EC cells systems. (tmc.edu)
  • We have determined in F9 cells that dissociating GalTase adhesion by galactosylation of and release of the consociate glycoproteins induces differentiation, as defined by increased laminin synthesis. (tmc.edu)
  • The ability to induce differentiation by surface galactosylation was greatest in cells grown in cultures promoting cell-cell adhesions, relative to cultures with minimal cell-cell interactions. (tmc.edu)
  • The presence of 'stem cell-like' cells has previously been postulated based on sphere-forming capacity and differentiation in vitro 1 and evidence for the presence of label-retaining cells in the distal fallopian tube 2 . (nature.com)
  • Our data reveal a significant overlap between Notch-dependent genes in the fallopian epithelium and the defined 'stem cell signature' of the mouse intestine, suggesting the existence of a conserved pathway that regulates tissue renewal and directly controls cell fate specification and differentiation in the organoid by inhibiting cilliogenesis. (nature.com)
  • Mesenchymal stem cells (MSCs) have been widely tested for treating a variety of refectory medical indications such as type 1 diabetes mellitus, systemic lupus erythematosus, rheumatoid arthritis and Crohn's disease due to their multiple differentiation potentials and immunomodulation capability [1-5]. (researchsquare.com)
  • Although pHi was previously thought to be relatively constant as a homeostatic mechanism, we now know that pHi changes during normal cell cycle progression, cell migration, and cell differentiation. (ucsf.edu)
  • We also found that increased pHi is necessary for adult and embryonic stem cell differentiation as well as lineage specification (Ulmschneider et al. (ucsf.edu)
  • In previously reports , Plasmodium vivax was found to be able to directly inhibited erythroid cell proliferation and differentiation. (biomedcentral.com)
  • Moreover, in vitro cultures of erythroid cells derived from haematopoietic stem cells has demonstrated that P. vivax is able to directly inhibit erythroid cell proliferation and differentiation [ 22 ]. (biomedcentral.com)
  • miR-203 drives breast cancer cell differentiation. (medscape.com)
  • Recent advances in live imaging and genetics of mammalian division, movement and cell differentiation leading to development which integrate observations of biochemical tissue formation [14 ]. (lu.se)
  • Results: Fibroblastic meningioma exhibited upregulated PI3K/Akt and TGFβ signaling pathways, and accelerated G1/S progression cell cycle. (omicsdi.org)
  • UCK2 promoted cell cycle progression of HCC by preventing the degradation of mTOR protein and maintaining the stability of PDPK1 mRNA. (biomedcentral.com)
  • Increased levels of polyamines in cells are considered to be involved in cancer progression. (spandidos-publications.com)
  • Additionally, miR-146a silencing or restoration of FLAP led to a reduction of HepG2 cell proliferation, cell cycle progression, migration, and invasion. (biomedcentral.com)
  • Using gene manifestation profiling, practical siRNA library testing and reverse-phase protein arrays (RPPA), we buy Dihydrotanshinone I found that J2 cells and Y produced a cooperative effect on human being foreskin keratinocytes (HFKs), which resulted in suppression of TGF- signaling juxtaposed with up-regulation of changes in cell cycle progression, cell adhesion and motility. (cell-metabolism.com)
  • The RB1 gene is involved in the regulation of cell cycle progression and genomic stability. (atlasgeneticsoncology.org)
  • Accumulated data suggest that the predicted evolution of diabetic glomerulopathy is comprised of an early, transient mesangial cell proliferation and subsequent hypertrophy of these cells that herald the slow progression into glomerulosclerosis [ 2 ]. (hindawi.com)
  • Methods Melanoma cell lines and patient-derived tumor xenografts (PDTX) treated with pevonedistat were assessed for viability/apoptosis and tumor growth, respectively, to identify sensitive/resistant models. (nih.gov)
  • 0.3 μM) and induced apoptosis in a subset of melanoma cell lines. (nih.gov)
  • DNA replication and overall genomic stability are negatively impacted by rapid cell proliferation and is mitigated by dysregulation of DNA damage repair (DDR) and apoptosis pathways. (purdue.edu)
  • I limited the scope of this work to DDR, cell cycle, DNA replication, apoptosis, and MAPK associated genes. (purdue.edu)
  • New anticancer therapy strategies refer to the inhibition of CDK-cyclin complexes as an important target to prevent uncontrolled proliferation and induce apoptosis in cancer cells ( 2 ). (spandidos-publications.com)
  • Although purvalanol-induced cell cycle arrest and apoptotic cell death were demonstrated in prostate ( 5 ), breast ( 6 ) and colon cancer cells ( 7 ), the exact molecular mechanism of purvanol-induced apoptosis has not been elucidated yet. (spandidos-publications.com)
  • The ability of lovastatin, mevastatin and simvastatin to inhibit the melanoma cell proliferation was examined using cytotoxicity and apoptosis assays. (biomedcentral.com)
  • Besides the effect on cell migration, exogenous Tβ 4 was found to have multifunctional activities such as angiogenesis, anti-apoptosis, anti-oxidative stress and anti-inflammation ( 8 - 11 ), which emphasize its therapeutic potential in the repair of damaged tissues or wound healing. (spandidos-publications.com)
  • Our previously-obtained impressive results of highly increased C2C12 mouse myoblast adhesion to amine plasma polymers (PPs) motivated current detailed studies of cell resistance to trypsinization, cell proliferation, motility, and the rate of attachment carried out for fibroblasts (LF), keratinocytes (HaCaT), rat vascular smooth muscle cells (VSMC), and endothelial cells (HUVEC, HSVEC, and CPAE) on three different amine PPs. (nature.com)
  • Ito et al ( 2 ) showed that overexpression of Tβ 4 enhanced the formation of actin-based pseudopodia and cell motility in prostate cancer cells, providing direct evidence that Tβ 4 plays a role in cell migration. (spandidos-publications.com)
  • A complete of eight glomeruli had been noticed and five of these were regular aside from three glomeruli that demonstrated mesangial cell proliferation and synechiae adhesions. (nanoker-society.org)
  • In vitro , RSV attenuated high glucose-induced plasminogen activator inhibitor (PAI-1) expression and mesangial cell proliferation, as well as Akt and nuclear factor-kappa B (NF- κ B) activation. (hindawi.com)
  • These results suggest that RSV prevents diabetes-induced renal inflammation and mesangial cell proliferation possibly through Akt/NF- κ B pathway inhibition. (hindawi.com)
  • demonstrated that RSV prevented high glucose-induced kidney mesangial cell proliferation and fibronectin expression through inhibition of high glucose-induced JNK and NF- B activation, NADPH oxidase activity elevation, and reactive oxygen species production [ 10 ]. (hindawi.com)
  • In the present study, therefore, we aimed to determine whether RSV treatment attenuated renal inflammation and mesangial cell proliferation under diabetic condition both in vivo and in vitro . (hindawi.com)
  • Here we demonstrate the existence of adult stem cells in the human fallopian tube epithelium, which gives rise to a monolayer of differentiated epithelial cells in a complex 3D organoid in vitro . (nature.com)
  • Figure 1: Single fallopian epithelial cells give rise to organoids that can be maintained long term. (nature.com)
  • The bile ducts carry bile, a fluid that is produced by the liver cells (hepatocytes) and modified by the biliary lining (epithelial) cells as it flows through the ducts to the small intestine. (emedicinehealth.com)
  • Co-culture of human being main epithelial cells with irradiated 3T3 fibroblast feeder cells (J2 cells) and the Rho kinase inhibitor Y-27632 (Y) allows for the unrestricted growth of cells of epithelial source by the process termed conditional reprogramming. (cell-metabolism.com)
  • Recently, this co-culture technique was altered to include epithelial cells from prostate, buy Dihydrotanshinone I breast, trachea, liver and lung, wherein the use of J2 cells and the Rho kinase inhibitor Y-27632 (Y) resulted in the quick reprogramming of cells into immortalized karyotype-stable ethnicities [6, 7]. (cell-metabolism.com)
  • These conditionally reprogrammed epithelial cells indicated markers of adult stem cells, but not of embryonic and induced pluripotent stem cells, and appeared to consist of cell populations resembling their main tissue of source, making them ideal to study tissue regeneration, as well as normal physiologic and pathologic processes [8]. (cell-metabolism.com)
  • Similar alterations within the VDR signalling pathway are known to occur in the context of colon cancer, in which a diminished response of 1,25D3 signal is associated with increased expression of the epithelial mesenchymal transcription factor (EMT) SNAIL1, which can directly inhibit the activities of VDR and E-cadherin, resulting in epithelial cells to lose their polarity. (bl.uk)
  • In addition, extracellular, administered Tβ 4 promoted the cell migration of various types of cells including cardiomyocytes, human umbilical vein endothelial cells and conjunctival epithelial cells ( 4 - 6 ). (spandidos-publications.com)
  • Our study provides a comprehensive analysis of the synergistic cyto- and genotoxic effects resulted from short-term exposure of human lung epithelial cells to pristine (as manufactured) and user-tailored SWCNTs, as a function of their physicochemical properties. (cdc.gov)
  • A major aspect our research program is determining in molecular detail how intracellular (cytoplasmic) pH (pHi) dynamics regulates cell behaviors, with a focus on epithelial plasticity. (ucsf.edu)
  • MDR is often related to the expression of drug efflux transporters belonging to the ATP Binding Cassette (ABC) family, that pump drugs outside the cells limiting their intracellular accumulation and toxicity. (mdpi.com)
  • However, results of a recent study showed that Tβ 4 was rapidly internalized by cells, suggesting the involvement of an intracellular receptor in the effects of the peptide ( 7 ). (spandidos-publications.com)
  • In breast cancer, HSP90 is a molecular chaperone expressed in tumor cells when there exists an excess of unfolded protein, and its co-chaperone has been reported to induce expression of AGR2, so AGR2 may be used by the endoplasmic reticulum to assist with protein folding to alleviate proteotoxic stress. (wikipedia.org)
  • AGR2 may help regulate the protein and mRNA levels in a cell overall as well. (wikipedia.org)
  • We found that Ca 2+ and/or 1,25(OH) 2 D 3 stimulated CaSR promoter activity and CaSR protein expression in the human colon carcinoma CBS cells, which possessed a functional CaSR. (aacrjournals.org)
  • Development of a Pten-null LUSC mouse model revealed that tumors with PTEN loss were refractory to antiprogrammed cell death protein 1 (anti-PD-1), highly metastatic and fibrotic, and secreted TGFß/CXCL10 to promote conversion of CD4+ lymphocytes into regulatory T cells (Treg). (bvsalud.org)
  • An early event in the signal transduction pathway induced by these cell-cell interactions is the rapid inactivation of a flagellar protein kinase that phosphorylates a 48 kDa flagellar protein. (elsevierpure.com)
  • Immunoblot analysis indicated that expression of SksC was ubiquitous: this soluble protein was found in both flagella and cell bodies and was expressed throughout the cell cycle and gametogenesis. (elsevierpure.com)
  • rOmpA encodes a surface-expressed protein of SFG-rickettsiae that is important for adhesion to host cells ( 10 ). (cdc.gov)
  • I sought to use a network approach to leverage differential gene expression (DGE), gene ontology (GO) terms, and protein-protein interactions (PPI) data to determine synergistic drug combinations in cancer cell lines with disparate DDR backgrounds. (purdue.edu)
  • Uvomorulin, lysosome-associated membrane protein-1 (LAMP-1), and laminin were recognized by surface GalTase and are, therefore, potential components in GalTase-specific adhesions. (tmc.edu)
  • GalTase expression at both the transcription and protein levels were differentially regulated during the cell cycle, with surface expression greatest in the G1 phase. (tmc.edu)
  • For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. (angioproteomie.com)
  • Our work bridges protein electrostatics and structure with cell biology to reveal how pHi dynamics regulates cell behaviors through protonation of titrating amino acids as a post-translational modification to regulate protein structure and function (Schönichen et al. (ucsf.edu)
  • The high impact our pioneering work bridging protein electrostatics and cell biology is highlighted by our publication h-index of 46. (ucsf.edu)
  • RNA-seq, co-immunoprecipitation and RNA-binding protein immunoprecipitation were used to determine the mechanism of UCK2 promoting HCC. (biomedcentral.com)
  • Cyclin-dependent kinases (CDKs), are members of the serine-threonine protein kinase family and are responsible for taking control of cell cycle regulation in eukaryotic cells. (spandidos-publications.com)
  • Protein acts as a building block of cell and also plays a major role in growth, maintenance, enzyme regulation, cell signaling, and also acts as biocatalyst [ 10 ] . (encyclopedia.pub)
  • Abnormal expression of 5-Lipoxygenase Activating Protein (FLAP) has been detected in many tumor cells. (biomedcentral.com)
  • We demonstrated that miR-146a expression is overexpressed, while FLAP protein and mRNA are suppressed in hepatocellular carcinoma tissues and HepG2 cells compared to para-carcinoma tissues and HL-7702 cells. (biomedcentral.com)
  • Less than 0.1 EU per µg protein as determined by the LAL method. (biolegend.com)
  • ED 50 = 0.25 - 1.0 μg/mL as measured by the ability of immobilized protein to support the adhesion of U937 cells. (biolegend.com)
  • Additionally, approximately 66% of melanomas harbour the BRAFV600E mutation, which represents a constitutive signal of the mitogen-activated protein kinase (MAPK) that is known to phosphorylate a range of substrates, resulting in the regulation involved in hyper-proliferation and survival of transformed cells. (bl.uk)
  • In vitro, we show that 1,25D3 to modestly increase the expression of E-cadherin mRNA and protein in SK-MEL-28, but remained absent in A375, a cell line that expresses relatively higher levels of EMT transcription factors including SNAI1 and ZEB1. (bl.uk)
  • The mRNA and protein expression of matrix metalloproteinase 2 (MMP2) and vascular endothelial growth factor (VEGF) in Tβ4-treated RP cells was assessed by quantitative polymerase chain reaction (RT-qPCR) and western blot analysis, respectively. (spandidos-publications.com)
  • Tβ4 also increased the mRNA and protein expression of MMP2 and VEGF in RP cells. (spandidos-publications.com)
  • DeMarini, and Chapter 20, by Rice and cell death determine the size protein in several signal ing path- and Herceg). (who.int)
  • Mechanistically, we discovered a miR-455-5p/PD-L1 regulatory axis which drives chemotherapy resistance, hallmarks metastases with active IFN-γ response pathway (an inducer of PD-L1 expression), and impacts T cells viability and relative abundances in tumor microenvironment (TME). (bvsalud.org)
  • We are currently determining if modulation of these cytokines and transcription factors during the disease will increase viability. (omicsonline.org)
  • Fifteen micromoles of purvalanol induced a reduction in cell viability by 20 and 35% within 24 and 48 h, respectively. (spandidos-publications.com)
  • Cell viability, adhesion and migration of primary cultured RP cells were observed in the presence of Tβ4 at various concentrations ranging from 1 to 1,000 ng/ml. (spandidos-publications.com)
  • Tβ4 did not affect cell viability and adhesion, but RP cell migration was stimulated by Tβ4 at concentrations of 100 and 1,000 ng/ml. (spandidos-publications.com)
  • Our results show that the degree of cell transformation is a function of the physicochemical properties of the SWCNT, with the nanotube with higher length, higher metal content and larger agglomerate size reducing cell viability to a larger extent. (cdc.gov)
  • Such changes in cell viability are also complemented by changes in cell structure, cycle and cell-cell interactions, all responsible for maintaining cell fate. (cdc.gov)
  • This implies the importance of AGR2 in maintaining epithelial barrier function, which is supported by FOXA1 and FOXA2 molecules (transcription factors for epithelial goblet cells) which can activate the AGR2 promoter. (wikipedia.org)
  • We speculate that BRAFV600E has altered VDR gene targeting by inducing VDR translocation into the cytosol and/or its association capacity with its target molecules that usually results in the abrogation of cell proliferation, including β-catenin for its cytosolic re-distribution and ubiquitin ligase FBXW7 with which VDR corporately induce the degradation of genes involves in cell cycle arrest like c-myc. (bl.uk)
  • We demonstrated the striking difference in the resistance to trypsin treatment between endothelial and non-endothelial cells. (nature.com)
  • MiR-146a has also been shown to promote the angiogenic activity of endothelial cells in HCC patients [ 11 ]. (biomedcentral.com)
  • Additionally, DNAM-1 regulates monocyte extravasation through PVR expressed by endothelial cells. (biolegend.com)
  • Our scientists pursue every aspect of cancer research-from exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. (mskcc.org)
  • When keratinocytes Rabbit Polyclonal to CSTL1 are co-cultured with irradiated 3T3 fibroblast feeder cells (J2 cells) and growth factors [1, 4, 5], they undergo continuous replication over many decades resulting in their immortalization. (cell-metabolism.com)
  • Materials and Methods Cells Human being keratinocytes were cultured from discarded and de-identified neonatal foreskin cells. (cell-metabolism.com)
  • Psoriasis is a complex, chronic, multifactorial, inflammatory disease that involves hyperproliferation of the keratinocytes in the epidermis, with an increase in the epidermal cell turnover rate (see the image below). (medscape.com)
  • It is an autoimmune skin disorder characterized by the loss of intercellular adhesion of keratinocytes in the upper parts of the epidermis (acantholysis), resulting in the formation of superficial blisters. (medscape.com)
  • sensitive models were enriched for genes involved in DNA repair, replication and cell cycle regulation, while immune response and cell adhesion pathways were upregulated in resistant models. (nih.gov)
  • KEGG analysis also demonstrated that focal adhesion and ECM-receptor interaction pathways were activated in anaplastic meningioma. (omicsdi.org)
  • 105:14436) and the focal adhesion kinase FAK (Choi et al. (ucsf.edu)
  • Cellular senescence, which is morphologically characterized by an enlarged and flattened cell shape, was first described by Hayflick [ 9 ]. (hindawi.com)
  • Cellular senescence refers to active cells that eventually enter a state of irreversible growth arrest. (hindawi.com)
  • The increased resistance observed for the non-endothelial cell types was accompanied by an increased rate of cellular attachment, even though spontaneous migration was comparable to the control, i.e., to the standard cultivation surface. (nature.com)
  • We have revealed the design principles and functions of "pH sensors" described as endogenous proteins regulated within the cellular pH range, including guanine nucleotide exchange factors regulating cell polarity (Frantz et al. (ucsf.edu)
  • However, it is plausible that chronic infection by microorganisms or self-DNA released from necrotic or incompletely apoptosed cells, or possibly even cellular retrotransposons, may activate innate immune pathways that cause inflammation-aggravated autoimmunity ( 1 - 3 ). (aai.org)
  • Polarization is a fundamental cellular property that plays a vital role in various biological processes in multi-cellular as well as single-cell organisms. (elifesciences.org)
  • However, isolated reports showed that user-tailoring could induce deleterious effects in exposed cells, from decrease in cellular proliferation, to changes in cellular adhesion, generation of reactive oxygen species or phenotypical variations, just to name a few. (cdc.gov)
  • Before full implementation of SWCNTs is achieved, their toxicological profiles need to be mechanistically correlated with their physicochemical properties to determine how the induced cellular fate is related to the exposure conditions or samples' characteristics. (cdc.gov)
  • By identifying changes in active hallmarks involved in cell-cell connections and maintaining epithelial layer integrity, we also determine the role that short-term exposure to SWCNTs plays in the overall cellular fate and cellular transformation. (cdc.gov)
  • Background The neddylation pathway conjugates NEDD8 to cullin-RING ligases and controls the proteasomal degradation of specific proteins involved in essential cell processes. (nih.gov)
  • Urinalysis demonstrated red bloodstream cells 100/high power field and urine proteins was 3+. (nanoker-society.org)
  • As demonstrated on LF fibroblasts, the resistance to trypsin was similar in serum-supplemented and serum-free media, i.e., medium without cell adhesion-mediating proteins. (nature.com)
  • Based on all the results, the increased resistance to trypsinization of C2C12, LF, HaCaT, and VSMC cells on amine PPs can be explained most probably by a non-specific cell adhesion such as electrostatic interaction between the cells and amine groups on the material surface, rather than by the receptor-mediated adhesion through serum-derived proteins adsorbed on the PPs. (nature.com)
  • These findings suggest that parasite proteins are able to inhibit erythroid cell growth by down-regulation of ezrin phosphorylation, leading to ineffective erythropoiesis ultimately resulting in severe malarial anaemia. (biomedcentral.com)
  • Many molecular pathways in eukaryotic cells are modulated by specific signaling proteins that are controlled, by phosphorylation and dephosphorylation, through the activity of kinase and phosphatase enzymes. (biomedcentral.com)
  • The regulation of complex and dynamic signal transduction proteins contributes to the destination of targeting proteins and the signal transduction of cell growth, and exposure to parasites can also influence signaling pathways. (biomedcentral.com)
  • The samples were tested in U937 cell adhesion assay. (biolegend.com)
  • Antibody blocking assay demonstrated that DNAM-1 may play a role in polarization of T cells, activation and effectors of Th1 cells. (biolegend.com)
  • We examined the effects of statin drugs on in vitro proliferation, migration and invasion of melanoma cells. (biomedcentral.com)
  • Effects on cell migration and invasion were assessed using transwell invasion and migration chambers. (biomedcentral.com)
  • Lovastatin, mevastatin and simvastatin inhibited the growth, cell migration and invasion of HT144, M14 and SK-MEL-28 melanoma cells. (biomedcentral.com)
  • This study showed that miR-146a has a stimulatory role in HepG2 cells and promotes HepG2 cell migration and invasion by targeting FLAP mRNA. (biomedcentral.com)
  • Enhancement of RP cell migration and angiogenesis is likely to be involved in the promotion of wound healing. (spandidos-publications.com)
  • Upregulation of Tβ 4 by cDNA-mediated transfection was found to cause actin depolymerization in fibroblast cells ( 1 ), which demonstrated that the modulation of Tβ 4 affected the polymerization state of actin cytoskeleton, as well as other cell processes associated with the organization of actin cytoskeleton, such as cell migration. (spandidos-publications.com)
  • Colon cancer cells overexpressing Tβ 4 also exhibited enhanced cell migration, due in part to the activation of the Rac1 signaling pathway ( 3 ). (spandidos-publications.com)
  • The mechanism by which exogenous Tβ 4 influences cell migration remains to be elucidated. (spandidos-publications.com)
  • Therefore, many laboratories try with great effort to develop resorbable tissue scaffolds that could support the patient´s cells. (nature.com)
  • ECM provides mechanical support for cells and also determines the shape of tissue 9 . (nature.com)
  • Intended therapeutic targets, though important, are detected in only a fraction of signatures, and cell line tissue of origin has been identified as a confounding factor throughout the signature detection procedure. (biomedcentral.com)
  • In this study, FC analysis revealed that the number of Pax7(+) cells per milligram isolated from 50 mg of fresh tissue increased 36% 24 h after exercise-induced muscle injury (300 unilateral maximal eccentric contractions). (mcmaster.ca)
  • The latter findings point to the participation of Nogo-A/NgR1 signaling in the regulation of other aspects of growth, such as tissue expansion or turnover by cell proliferation. (jneurosci.org)
  • of the most critical events which lead to tissue patterning involves mechanisms going beyond single cells. (lu.se)
  • The misplaced endometrial tissue can irritate nearby tissues, causing bands of scar tissue (adhesions) to form between structures in the abdomen. (msdmanuals.com)
  • DNAM-1 is predominantly expressed on cell surface of T cells, NK cells, monocytes/ macrophages, platelets and megakaryocytes and a subset of B cells. (biolegend.com)
  • Several evidences demonstrated that DNAM-1 is involved in NK and T cell-mediated cytotoxicity (CTL) against ligand-expressing tumor cells. (biolegend.com)
  • However, MSCs need to be expanded in vitro in order to obtain sufficient cells for clinical trials since they are extremely rare in various tissues. (hindawi.com)
  • EGFL6 mRNA levels were also determined in brain arachnoidal tissues, meningiomas, and other tumors by qRT-PCR. (omicsdi.org)
  • The largest cell panels available today are derived from cancerous tissues, since a crucial step towards personalized cancer medicine is the identification of transcriptional signatures that can guide drug prescription. (biomedcentral.com)
  • Herein, we aimed to demonstrate the expression of miR-146a and FLAP in human HCC tissues and liver cancer cell lines. (biomedcentral.com)
  • Tβ 4 is distributed ubiquitously in most tissues and cells, and is also known to concentrate highly at blood platelets ( 12 ). (spandidos-publications.com)
  • Deep molecular characterization of immune cells, cytolytic-activity and tumor-inflammatory status revealed diversity of the local immune infiltration landscape in the melanoma clusters. (jcancer.org)
  • DNAM-1, also known as CD226, was first identified as T lineage-specific activation antigen (TLiSA) and subsequently renamed as Platelet and T-cell activation antigen 1 (PTA). (biolegend.com)
  • GalTase-specific adhesions are functional in a number of important biological events, including F9 embryonal carcinoma (EC) cell adhesions. (tmc.edu)
  • Immune cell infiltration level and immune-related functional scores were evaluated to assess the link between tumour microenvironment and UCK2. (biomedcentral.com)
  • Comparison of the structural and functional specialisations of cells lining the stomach with those of the cells lining the small intestine. (markedbyteachers.com)
  • Mesenchymal stem cells (MSCs) possess great therapeutic potential. (hindawi.com)
  • To compare the heterogeneities of human umbilical cord mesenchymal stem cells (HUCMSCs) derived from different donors and test their therapeutic variations in mouse liver fibrosis model. (researchsquare.com)
  • Downregulation of UCK2 induced cell cycle arrest and activated the TNFα/NFκB signalling pathway-related senescence-associated secretory phenotype to modify the tumour microenvironment. (biomedcentral.com)
  • In the present study, we found that knockdown of UCK2 induced cell cycle arrest through dual mechanisms in HCC and that targeting UCK2 promoted the secretory features involved in the senescence-associated secretory phenotype (SASP) and inflammasomes. (biomedcentral.com)
  • Purvalanol, a novel cyclin-dependent kinase inhibitor, is referred to as a strong apoptotic inducer which causes cell cycle arrest in various cancer cells such as prostate, breast and colon cancer cell lines. (spandidos-publications.com)
  • Purvalanol is a purine-derived CDKI that binds with a high selectivity and competitively to the ATP binding side of CDK1/2 and leads to G2/M cell cycle arrest ( 4 ). (spandidos-publications.com)
  • Astrocytes bear multiple vital functions such as maintaining the ion homeostasis, contributing to the blood-brain barrier, restoring synaptic integrity, regulating immune response, and acting as neural stem cells ( Kettenmann and Ransom, 2012 ). (frontiersin.org)
  • Natural polyamines, putrescine, spermidine and spermine, play essential roles in the regulation of cell growth and proliferation. (spandidos-publications.com)
  • Using gene removal as a model of inhibition, I measured resulting network disruption to determine synergistic relationships. (purdue.edu)
  • Microarray analysis reveals that inhibition of Notch signalling causes downregulation of stem cell-associated genes in parallel with decreased proliferation and increased numbers of ciliated cells and that organoids also respond to oestradiol and progesterone treatment in a physiological manner. (nature.com)
  • The HUCMSCs derived different donors have individual heterogeneity, which potentially lead to distinct therapeutic outcomes in mouse liver fibrosis, indicating we could make use of the donor-variation of MSCs to screen out guaranteed general indicators of MSCs for specific diseases in further stem cell therapy. (researchsquare.com)
  • Our hospital is approved to be qualified for implementing stem cell clinic trials by government agencies [19]. (researchsquare.com)
  • Outside the stem cell niches, however, these glial cells are not neurogenic. (frontiersin.org)
  • and stem cell regeneration. (lu.se)
  • interactions between cells. (lu.se)
  • Gene expression microarray and gene set enrichment analyses were performed in cell lines to determine the expression profiles and pathways of sensitivity/resistance. (nih.gov)
  • Proliferating cell nuclear antigen (PCNA) is an essential gene to numerous tumor-dysregulated processes including DNA replication and repair. (purdue.edu)
  • Conventional wisdom would prohibit targeting PCNA due to its status as an essential gene, as directly antagonizing it would cause toxic effects in healthy cells. (purdue.edu)
  • HCT 116 colon cancer cells were exposed to purvalanol, which activated ER stress via upregulation of PERK, IRE1α gene expression, eIF-2α phosphorylation and ATF-6 cleavage at early time-points in the HCT 116 colon cancer cells. (spandidos-publications.com)
  • We apply our approach to 189 drugs tested in 671 cancer cell lines and observe a connection between gene expression levels of the modules and mechanisms of action of the drugs. (biomedcentral.com)
  • Gene expression profiling has become a mainstay approach to characterize cell properties and status, unveiling links between gene activities and disease phenotypes. (biomedcentral.com)
  • Provided that the panel of cell lines is large enough, this approach allows for a new type of gene expression analysis where basal expression levels are correlated to drug response phenotypes. (biomedcentral.com)
  • The pathogenic role of del(13q) in CLL has been related to lack of B-cell proliferation control allegedly determined by deletion of the DLEU2 / MIR15A / MIR16-1 locus, which is known to contain negative regulators of the expression of the BCL2 gene (Cimmino et al. (atlasgeneticsoncology.org)
  • Pevonedistat also reduced tumor growth in melanoma cell line xenografts and PDTX with variable responses. (nih.gov)
  • We established a Good Manufacturing Practice (GMP) grade cell facility to produce clinic-grade human umbilical cord derived MSCs (HUCMSCs) for treating premature ovarian failure (POF) and recurrent uterine adhesion [18, 20]. (researchsquare.com)
  • Other sequelae associated with PID include dyspareunia, pyosalpinx, tubo- ovarian abscess, and pelvic adhesions (6). (cdc.gov)
  • The radiographer should get the patients history as they may determine exposure factors or inidcate any varitations that may be needed. (markedbyteachers.com)
  • This technique was employed recently to examine the differential drug response of cells acquired from a patient with recurrent respiratory papillomatosis to identify a drug that was effective in controlling this disease [9]. (cell-metabolism.com)
  • Additionally, immunohistochemistry results indicated that RSV treatment reduced the density of proliferating cell nuclear antigen-positive cells significantly in glomeruli of diabetic mice. (hindawi.com)
  • Bacterial DNA and RNA: We have determined that bacterial DNA and RNA induce a depression of myocyte contraction. (omicsonline.org)
  • Unlike embryonic stem cells, adult stem cells (MSCs) have a limited lifespan and stop proliferating during in vitro culture due to replicative senescence [ 8 ]. (hindawi.com)
  • The molecular mechanisms mediating pHi-regulated cell behaviors, however, remain understudied and largely unknown. (ucsf.edu)
  • Therefore, finding out molecular mechanisms by which reactive astrocytes can be coaxed into neurons will be of utmost importance for regenerative therapies as these astrocytes are the imminent cell types around the lesion site. (frontiersin.org)
  • In this study, a phosphoproteomic approach was performed to dissect the molecular mechanism of phosphoprotein regulation, which is involved in the inhibitory effect of parasites on erythroid cell development. (biomedcentral.com)
  • The taxonomic positions of these bacteria have been determined with much greater scientific clarity by the recent application of molecular methods, which were critical for the identification of E. chaffeensis and essential for that of human granulocytic ehrlichia (HGE) ( 1 , 2 ). (cdc.gov)
  • Here, we reconstructed Par-dependent polarity using non-polarized Drosophila S2 cells expressing all three components endogenously in the cytoplasm. (elifesciences.org)
  • Thus, this reconstruction system provides an experimental paradigm to study features of the assembly process and structure of Par-dependent cell-autonomous polarity. (elifesciences.org)
  • We used Drosophila Schneider cells (S2 cells) of mesodermal origin, as host cells for cell-autonomous reconstruction of cell polarity ( Schneider, 1972 ). (elifesciences.org)
  • With this goal in mind, we wanted to determine the signaling pathways associated with J2 cells and Y that are paramount to the immortalization process. (cell-metabolism.com)
  • Here we report on the establishment of long-term, stable 3D organoid cultures from human fallopian tubes, indicative of the presence of adult stem cells. (nature.com)
  • In the adult mammalian subventricular zone (SVZ), GFAP-positive neural stem cells (NSCs) generate neuroblasts that migrate tangentially along the rostral migratory stream (RMS) toward the olfactory bulb (OB). (jneurosci.org)
  • This result suggests that phospho-ezrin is important for actin cytoskeleton regulation during erythroid cell growth and division. (biomedcentral.com)
  • Fertilization in the biflagellated eukaryote, Chlamydomonas, is initiated by flagellar adhesion between gametes of opposite mating types. (elsevierpure.com)
  • It is lined by simple columnar epithelium containing secretory and ciliated cells, which produce tubular fluid and facilitate transport of gametes, respectively. (nature.com)
  • In this study, we show that the cells of the colon crypt acquire CaSR expression as they differentiate and migrate towards the apex of the crypt. (aacrjournals.org)
  • The median cluster was characterized by an inability to draw immune cells, highlighting possible immune escape mechanisms, and lower CXCL9 and CXCL10 expression, which was correlated to poor prognosis. (jcancer.org)
  • These data indicated that the increase in cell adhesions expected with increased cell-matrix interaction in differentiated F9 cells is not the consequence of increased surface GalTase expression and, more interestingly, that the two pools of GalTase are under differential regulation. (tmc.edu)
  • We also used a machine learning approach to identify robust BCNHL biomarkers that include YES1, FERMT2, and FAM98B, which have not previously been associated with BCNHL in the literature, but together provide ~99.9% combined specificity and sensitivity for differentiating lymphoma cells from healthy B-cells based on measurement of transcript expression levels in B-cells. (fortunepublish.com)
  • Knockdown of miR-146a also resulted in increased FLAP expression of cancer cells. (biomedcentral.com)
  • A knockdown of SNAI1 and, independently of ZEB1, in A375 cells is insufficient to recover E-cadherin expression. (bl.uk)
  • We also determined the absence of E-cadherin expression in A375 to not be regulated by methylation. (bl.uk)
  • Astrocytes are abundant cell types in the vertebrate central nervous system and can act as neural stem cells in specialized niches where they constitutively generate new neurons. (frontiersin.org)
  • The concentrations required to inhibit proliferation of melanoma cells (0.8-2.1 μM) have previously been achieved in a phase I clinical trial of lovastatin in patients with solid tumours, (45 mg/kg/day resulted in peak plasma concentrations of approximately 3.9 μM). (biomedcentral.com)
  • Two ligands for DNAM-1 have been identified, the poliovirus receptor (PVR/CD155) and its family member nectin-2 (PRR/CD112), which are located at cell junctions and broadly expressed on epithelial, endothelial and neuronal cells. (biolegend.com)
  • Particularly affected was ezrin (phosphorylated at Thr567), which is normally localized to gEC cell extension peripheral processes. (biomedcentral.com)
  • Cell polarization involving the Par-complex in situ is linked to various other processes. (elifesciences.org)
  • Because of such association between Par-dependent polarization and other processes, the Par-complex exhibits different behavioral characteristics in an individual context, making it difficult to determine general features of the dynamic process taking place during cell polarization by the Par-complex. (elifesciences.org)
  • Immunotherapy resistance in non-small cell lung cancer (NSCLC) may be mediated by an immunosuppressive microenvironment, which can be shaped by the mutational landscape of the tumor. (bvsalud.org)
  • Downregulated UCK2 induced a secretory phenotype, which could improve the microenvironment, and decreased UCK2 remodelling metabolism could lower the resistance of tumour cells to T-cell-mediated killing. (biomedcentral.com)