Presence of tunicamycinMoleculesThapsigarginMoleculeTransmembraneTransientMDCKGlycosylationBovineMonoclonalGrowthAntibodiesProteinMammalian cellsEpithelial cellsTreatmentTissuesAbnormalApoptosisImmunofluorescenceMorphogenesisEndoplasmic reticulumTissueTumorPathwaysVivoBiolGlycoproteinsDefectiveMechanismProliferationCellularCyclosporinHumanDifferentialLargeDeathHighRoleSimilar
Presence of tunicamycin2
- The monoclonal antibodies reacted with neu protein on intact cells by immunofluorescence and immunoprecipitated neu in metabolically labeled cells, also in the presence of tunicamycin. (prinsesmaximacentrum.nl)
- The glycosylation of GPIIb-IIIa was examined in megakaryocytes by metabolic labeling in the presence of tunicamycin, monensin, or treatment with endoglycosidase H. The polypeptide backbones of the GPIIb and the GPIIIa have molecular masses of 120 and 90 kD, respectively. (rupress.org)
Molecules8
- The desmosomal glycoproteins appear to bind calcium, as shown by 45 Ca blotting, while the desmosomal adhesion molecules yield a soluble, 42,000 molecular weight, glycosylated calcium protected fragment when treated with trypsin. (soton.ac.uk)
- In animals, cadherin domain-containing proteins are adhesion molecules that modulate a wide variety of processes including cell polarization and migration but they have also been identified in yeast and magnetotactic bacteria. (embl.de)
- The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
- Cell adhesion molecules. (academicinfluence.com)
- Cell adhesion molecules: implications for a molecular histology. (academicinfluence.com)
- Cell adhesion molecules in the regulation of animal form and tissue pattern. (academicinfluence.com)
- Cell adhesion molecules in early chicken embryogenesis. (academicinfluence.com)
- Binding of neural cell adhesion molecules (N-CAMs) to the cellular prion protein. (academicinfluence.com)
Thapsigargin1
- Interestingly, bacterial invasion was facilitated by knockdown of UPR-associated signaling factors and treatment with the ER stress inducers, thapsigargin and tunicamycin, decreased the invasive ability of C. jejuni. (tokushima-u.ac.jp)
Molecule7
- The ß2 subunit of Na+, K+-ATPase was originally identified as the adhesion molecule on glia (AMOG) that mediates the adhesion of astrocytes to neurons in the central nervous system and that is implicated in the regulation of neurite outgrowth and neuronal migration. (bvsalud.org)
- While ß1 isoform have been shown to trans-interact in a species-specific mode with the ß1 subunit on the epithelial neighboring cell, the ß2 subunit has been shown to act as a recognition molecule on the glia. (bvsalud.org)
- In the present report we designed experiments that would clarify whether ß2 is a cell-cell homophilic adhesion molecule. (bvsalud.org)
- Neural cell adhesion molecule: structure, immunoglobulin-like domains, cell surface modulation, and alternative RNA splicing. (academicinfluence.com)
- Kinetics of homophilic binding by embryonic and adult forms of the neural cell adhesion molecule. (academicinfluence.com)
- Chemical characterization of a neural cell adhesion molecule purified from embryonic brain membranes. (academicinfluence.com)
- Purification and characterization of a cell adhesion molecule from neural retina. (academicinfluence.com)
Transmembrane1
- We also used NIH3T3 cells transfected with either a normal or a mutated human neu gene, encoding a protein with a valine to glutamic acid substitution in the transmembrane domain. (prinsesmaximacentrum.nl)
Transient1
- Pre-application of ethanol, hydrogen peroxide (H 2 O 2 ) and LOOH to S. cerevisiae cells reduced the effect of LOOH on the cells, showing the transient cross adaptation to LOOH. (go.jp)
MDCK4
- The desmosomal components of frog and chicken skin, Madin Darby bovine and canine kidney (MDBK and MDCK) cells and human keratinocytes were immunoblotted with these antisera. (soton.ac.uk)
- A similar assembly of desmosomal antigens, illustrated by immunofluorescence, could be demonstrated in MDCK cells treated with tunicamycin in low calcium medium. (soton.ac.uk)
- Protein-protein interaction assay in vivo with MDCK (Madin-Darby canine kidney) or CHO cells expressing a recombinant ß2 subunit show that the ß2 subunits on the cell surface of the transfected cell lines interact with each other. (bvsalud.org)
- Overall, our results suggest that the human ß2 subunit can form trans-dimers between neighboring cells when expressed in non-astrocytic cells, such as fibroblasts (CHO) and epithelial cells (MDCK). (bvsalud.org)
Glycosylation2
- N-linked glycosylation is the most prevalent posttranslational modification of plasma membrane and secretory proteins and participates in many important biological roles such as protein folding, intracellular targeting, immune response, cell adhesion, and protease resistance. (jneurosci.org)
- Treating cells with glycosylation inhibitors (e.g., tunicamycin to block N-linked glycosylation or Benzyl-α-GalNAc (BαG) to block O-glycosylation) can be used to generate the unmodified target protein. (genetex.com)
Bovine2
- Interestingly, expression of similar bovine rhodopsin mutants did not cause rod cell death, possibly attributable to greater stability of bovine rhodopsin. (jneurosci.org)
- In COS cells, bovine T4K and T17M rhodopsins only partially regenerate with chromophore ( Kaushal and Khorana, 1994 ). (jneurosci.org)
Monoclonal3
- We have obtained monoclonal antibodies to the human neu protein by immunizing a Balb/c mouse with a Balb/c cell line expressing the human neu gene by transfection. (prinsesmaximacentrum.nl)
- We tested possible down-modulating effects of these monoclonal antibodies on SKBR-3 mammary tumor cells, which express high levels of wild-type human neu protein. (prinsesmaximacentrum.nl)
- Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.cn)
Growth5
- The exposure of Saccharomyces cerevisiae cells to 13- L -hydroperoxylinoleic acid (LOOH) caused their death, the degree of which was dependent on the growth phase of the cells. (go.jp)
- Our results demonstrate that tumor growth, peritoneal dissemination and peritoneum or organ metastasis implanted MKN45 cells were significantly decreased in shAhR and Biseugenol-treated mice and that endoplasmic reticulum (ER) stress was caused. (oncotarget.com)
- Insulin is a peptide hormone secreted by the pancreas that regulates glucose homeostasis, and high levels of circulating insulin can activate the PI3K-AKT-mTOR pathway which promotes growth in cancer cells [ 12 ]. (biomedcentral.com)
- Metabolic alterations in cancer cells are numerous and include aerobic glycolysis, reduced oxidative phosphorylation, and increased generation of biosynthetic intermediates needed for cell growth and proliferation. (biomedcentral.com)
- Surface modulation in cell recognition and cell growth. (academicinfluence.com)
Antibodies2
- In contrast, the antibodies induced 50-65% down-modulation in NIH3T3 cells expressing the mutated human neu protein and could inhibit these cells to form colonies in soft agar. (prinsesmaximacentrum.nl)
- Immunoprecipitation of [35S]methionine pulse-chase-labeled cell extracts by antibodies specific for the alpha or beta subunits of GPIIb indicated that GPIIb was derived from a precursor of Mr 130,000 that contains the alpha and beta subunits. (rupress.org)
Protein5
- For this purpose, we performed protein docking analysis, cell-cell aggregation, and protein-protein interaction assays. (bvsalud.org)
- Down-modulation of the normal cell-surface neu protein was inefficient. (prinsesmaximacentrum.nl)
- In some conditions, especially in tumor microenvironment, cells undergo nutrient deprivation, hypoxia or drug-induced toxicity, protein folding is interfered. (biomedcentral.com)
- The protein is present as a patch at the incipient bud site and in emerging buds, and at the bud periphery in small-budded cells. (embl.de)
- We identified interferon signaling, viral response, and protein targeting pathways that are induced by insulin but dependent on Viperin in PIK3CA H1047R mutant cells. (biomedcentral.com)
Mammalian cells2
- However, the DNA fragmentation characteristic of apoptosis was not observed in S. cerevisiae cells after exposure to LOOH, staurosporine, dexamethasone or etoposide, which have been reported to cause apoptosis in mammalian cells. (go.jp)
- To counteract DNA damage, mammalian cells have evolved partially overlapping DNA repair systems to remove DNA lesions and restore their DNA back to its native form 1 , 2 . (nature.com)
Epithelial cells3
- Hyperinsulinemia also promotes cancer, but the direct action of insulin on mutated endometrial epithelial cells is unknown. (biomedcentral.com)
- Here, we treated 12Z endometriotic epithelial cells carrying the PIK3CA H1047R oncogene with insulin and examined transcriptomes by RNA-seq. (biomedcentral.com)
- These results suggest that response to insulin signaling is altered in mutated endometriotic epithelial cells. (biomedcentral.com)
Treatment8
- This treatment also caused patching of desmocollins in MDBK cells. (soton.ac.uk)
- The treatment with Tunicamycin in vivo reduced cell aggregation, suggesting the participation of N-glycans in that process. (bvsalud.org)
- Cell proliferation was inhibited after cisplatin treatment when ERp44 was knocked down and ERp44 strengthened cisplatin resistance by influencing cell apoptosis and pyroptosis. (biomedcentral.com)
- Then we also collected exosomes and cell viability was increased after the addition of NPC-derived-exosomes with cisplatin treatment. (biomedcentral.com)
- Our data suggested that exosomal ERp44 derived from ER-stressed NPC cells took an inevitable role in NPC chemoresistance and might act as a treatment target. (biomedcentral.com)
- Tunicamycin treatment experiments, biochemical fractionation and extraction experiments, and proteinase K protection experiments collectively indicate that Axl2p is an integral membrane glycoprotein at the plasma membrane. (embl.de)
- Insulin treatment in wild-type cells activated normal endoplasmic reticulum stress (ERS) response programs, while PIK3CA H1047R cells activated programs necessary to avoid ERS-induced apoptosis. (biomedcentral.com)
- The transcriptional changes induced by insulin in PIK3CA H1047R cells were rescued by knockdown of Viperin, while Viperin OE alone was insufficient to induce a DGE response to insulin, suggesting that Viperin is necessary but not sufficient for the synergistic effect of PIK3CA H1047R and insulin treatment. (biomedcentral.com)
Tissues1
- Vascular endothelial cells (ECs) form a critical interface between blood and tissues that maintains whole-body homeostasis. (bvsalud.org)
Abnormal2
- The syndrome is characterized by abnormal adhesion-dependent functions, especially defective tissue emigration of neutrophils, leading to recurrent infection. (lookformedical.com)
- Give the links between abnormal endometrium and conditions associated with hyperinsulinemia, we hypothesized that insulin may have a direct impact on endometrial epithelia, and that the response to insulin would be altered in cells that have acquired a PIK3CA mutation. (biomedcentral.com)
Apoptosis2
- Bacterial entry into a host eukaryotic cell involves the initial steps of adherence and invasion, which generally activate several cell-signaling pathways that induce the activation of innate defense systems, which leads to the release of proinflammatory cytokines and induction of apoptosis. (tokushima-u.ac.jp)
- Flow cytometric analysis and western blot were taken to analyze cell apoptosis. (biomedcentral.com)
Immunofluorescence1
- 0.1mM), were treated with tunicamycin desmosome formation was triggered, as shown by immunofluorescence and electronmicroscopy. (soton.ac.uk)
Morphogenesis2
- Spa2p and Cdc10p both participate in bud site selection and cell morphogenesis in yeast, and spa2delta cdc10-10 cells are inviable. (embl.de)
- Cell adhesion and the molecular processes of morphogenesis. (academicinfluence.com)
Endoplasmic reticulum1
- Our comparative proteomic analysis of HCC cells grown in culture as monolayers (2D) and spheroids (3D) revealed that argininosuccinate synthase 1 (ASS1) expression was higher in 3D cells than in 2D cells due to upregulated endoplasmic reticulum (ER) stress responses. (biomedcentral.com)
Tissue1
- Tissue, cells or virus corresponding to O-Linked N-Acetylglucosamine. (abcam.cn)
Tumor1
- These results demonstrate the critical functional roles for the arginine metabolism-independent tumor suppressor activity of ASS1 in HCC and suggest that upregulating ASS1 in these tumors is a potential strategy in HCC cells with low ASS1 expression. (biomedcentral.com)
Pathways1
- In vitro, studies in human umbilical vein endothelial cells (HUVECs) and in silico network pharmacology analyses were performed to reveal the underlying mechanisms of GQP and determine the main components, targets, and pathways of GQP, respectively. (bvsalud.org)
Vivo2
- Presently, two-dimensional (2D) cell-based models fail to predict in vivo efficacy, contributing to limited success in translating new drugs for clinical use. (biomedcentral.com)
- STUDY DESIGN: We tested our hypothesis in a carrageenan-induced thrombosis mouse model in vivo and lipopolysaccharide (LPS)-induced human endothelial cells (HUVECs) in vitro. (bvsalud.org)
Biol1
- J Cell Biol (1987) 104 (6): 1665-1673. (rupress.org)
Glycoproteins2
- Fucose is a monosaccharide that is commonly found in the cell walls of bacteria and is involved in the formation of glycoproteins and glycolipids. (lookformedical.com)
- Rare, autosomal recessive disorder caused by deficiency of the beta 2 integrin receptors (RECEPTORS, LEUKOCYTE-ADHESION) comprising the CD11/CD18 family of glycoproteins. (lookformedical.com)
Defective1
- axl2 cells are defective in bud site selection in haploid cells and bud in a bipolar fashion. (embl.de)
Mechanism2
- An investigation into the mechanism of UPR-mediated inhibition of C. jejuni invasion showed that UPR signaling did not affect bacterial adhesion to or survival in the host cells. (tokushima-u.ac.jp)
- Nevertheless, none of the works have identified the binding partner of ß2 or described its adhesion mechanism. (bvsalud.org)
Proliferation1
- In our previous research, we have also reported that ERp44 was highly expressed in NPC and associated with patients' survive state and clinical stages, it also participated in promoting cells proliferation and migration [ 22 ]. (biomedcentral.com)
Cellular1
- The Er1 F/− EV cargo is taken up by recipient cells leading to an increase in insulin-independent glucose transporter levels, enhanced cellular glucose uptake, higher cellular oxygen consumption rate and greater tolerance to glucose challenge in mice. (nature.com)
Cyclosporin1
- Antioxidants such as N , N ′,-diphenyl- p -phenylenediamine (DPPD), melatonin and vitamin E, and inhibitors of permeability transition of mitochondria, cyclosporin A and trifluoperazine, inhibited the LOOH-triggered cell death, while an inhibitor of glutathione synthetase, buthionine sulfoximine (BSO), enhanced the cell death by LOOH. (go.jp)
Human2
- To examine the biosynthesis and processing of GPIIb-IIIa, purified human megakaryocytes were isolated from liquid cultures of cryopreserved leukocytes stem cell concentrates from patients with chronic myelogenous leukemia. (rupress.org)
- CypD knockdown (KD) significantly inhibited the binding of NOX 4 to Poldip 2, and mitochondrial ROS generation in human umbilical vein endothelial cells (HUVECs). (bvsalud.org)
Differential2
- We performed differential centrifugation to isolate exosomes from serum or conditioned media of cells and analyzed the impact of exosomal ERp44 on cells cisplatin sensitivity. (biomedcentral.com)
- While cells naively responded to insulin, the magnitude of differential gene expression (DGE) was nine times greater in PIK3CA H1047R cells, representing a synergistic effect between insulin signaling and PIK3CA H1047R expression. (biomedcentral.com)
Large2
- Congenital MEGACOLON resulting from the absence of ganglion cells (aganglionosis) in a distal segment of the LARGE INTESTINE. (lookformedical.com)
- In cells containing medium-sized or large buds, Axl2p is located as a ring at the neck. (embl.de)
Death2
- Retinal degeneration is progressive deterioration of the retinal cells, eventually culminating in their death. (molvis.org)
- These reactive radicals possibly induced the death of S. cerevisiae cells. (go.jp)
High1
- We find that high glucose in EV-targeted cells triggers pro-inflammatory stimuli via mTOR activation. (nature.com)
Role1
- We took CCK8 to detect the role of ERp44 on cell chemosensitivity. (biomedcentral.com)
Similar1
- In isolated frog retinas treated with tunicamycin, rods synthesized and transported nonglycosylated rhodopsin to the basal ROS at a rate similar to untreated controls. (jneurosci.org)