• Localization of a domain in the FimH adhesin of Escherichia coli type 1 fimbriae capable of receptor recognition and use of a domain-specific antibody to confer protection against experimental urinary tract infection. (jci.org)
  • The FimH subunit of type 1-fimbriated Escherichia coli has been implicated as an important determinant of bacterial adherence and colonization of the urinary tract. (jci.org)
  • Here, we sought to localize the functionally important domain(s) within the FimH molecule and to determine if antibodies against this domain would block adherence of type 1-fimbriated E. coli to the bladder mucosa in situ and in vivo in an established mouse model of cystitis. (jci.org)
  • Next, we compared the ability of mice passively immunized intraperitoneally with antisera raised against residues 1-25 and 253-264 of FimH or 1-13 of FimA to resist bladder colonization in vivo after intravesicular challenge with type 1-fimbriated E. coli. (jci.org)
  • Only the antibody directed at the putative binding region of FimH (anti- s-FimH1-25) significantly reduced E. coli bladder infections in the experimental mouse model of urinary tract infections. (jci.org)
  • The best characterized bacterial adhesin is the type 1 fimbrial FimH adhesin. (wikipedia.org)
  • Escherichia coli is the most common pathogen associated with urinary tract infection (UTI) (5, 10, 30). (scielo.br)
  • 150 women with urinary tract infection caused by Escherichia coli randomly allocated into three groups. (bmj.com)
  • Women who had a urinary tract infection caused by Escherichia coli (≥10 5 colony forming units/ml in clean voided midstream urine) and were not taking antimicrobial prophylaxis were invited to participate. (bmj.com)
  • Since common urinary tract-infection bacteria such as E. coli dislike acidic surroundings, physicians concluded that they had discovered a scientific explanation for the traditional uses of cranberry. (epnet.com)
  • Thus, UpaG is a novel trimeric AT adhesin from E. coli that mediates aggregation, biofilm formation, and adhesion to various ECM proteins. (edu.au)
  • Additionally, AIDA expression dramatically enhances biofilm formation by E. coli on abiotic surfaces in How chambers. (forskningsdatabasen.dk)
  • We investigated the intestinal adherence of E. coli with Dr hemagglutinin, S fimbriae, CFA/I or CFA/II, using freshly isolated ileal or colonic enterocytes and cells from the human colonic cell line HT-29. (gu.se)
  • Adhesins are cell-surface components or appendages of bacteria that facilitate adhesion or adherence to other cells or to surfaces, usually the host they are infecting or living in. (wikipedia.org)
  • To effectively achieve adherence to host surfaces, many bacteria produce multiple adherence factors called adhesins. (wikipedia.org)
  • In addition, the distribution of adhesin-encoding operons between the phylogroups was assessed. (nih.gov)
  • These results indicate that the PCR method is a powerful genotypic assay for the detection of adhesin-encoding operons. (nih.gov)
  • Additionally, Adhesins are attractive vaccine candidates because they are often essential to infection and are surface-located, making them readily accessible to antibodies. (wikipedia.org)
  • This is the first characterization and demonstration of an Afa adhesin associated with EPEC. (asm.org)
  • Prevalence analysis revealed that upaG is strongly associated with E. coli strains from the B2 and D phylogenetic groups, while deletion of upaG had no significant effect on the ability of CFT073 to colonize the mouse urinary tract. (edu.au)
  • In this study the development of a PCR-based technique for the rapid identification of the food-borne pathogens Salmonella and Escherichia coli was undertaken. (biomedsearch.com)
  • Pathogenic strains of Escherichia coli are the most common bacteria associated with urinary tract infections in both humans and companion animals. (scielo.br)
  • Adhesins are expressed by both pathogenic bacteria and saprophytic bacteria. (wikipedia.org)
  • The study of adhesins as a point of exploitation for vaccines comes from early studies which indicated that an important component of protective immunity against certain bacteria came from an ability to prevent adhesin binding. (wikipedia.org)
  • These specificities may contribute to the establishment of E. coli in the intestinal microflora, which precedes their spread to extra-intestinal sites. (gu.se)
  • Hybridization with a DNA probe ( daaC probe) developed to detect DA E. coli (DAEC) ( 5 ) indicated that such strains possess a specific DA adhesin. (asm.org)
  • These studies have established that fimA and afa primers were specific for detecting Salmonella and pathogenic E. coli, respectively, in the environmental samples. (biomedsearch.com)
  • An atomic resolution model for assembly, architecture, and function of the Dr adhesins. (ebi.ac.uk)