• The adenylate cyclase/cyclic AMP/protein kinase A (PKA) module is a central component of such biochemical signaling. (harvard.edu)
  • I. Activation of Adenylate Cyclase by 5-Hydroxytryptamine. (erowid.org)
  • Fasciola hepatica were collected from fresh beef livers and particulate fractions of whole flukes, 'tails' or 'heads' (anterior ends) assayed for adenylate cyclase (AC) activity and cyclic AMP concentration using a protein binding assay. (erowid.org)
  • Basal and epinephrine-stimulated adenylate cyclase and glycogen phosphorylase as well as binding of cyclic AMP to protein kinase were unaltered. (diabetesjournals.org)
  • Many heterologously expressed mutants of the cystic fibrosis transmembrane conductance regulator (CFTR) exhibit residual chloride channel activity that can be stimulated by agonists of the adenylate cyclase/protein kinase A pathway. (nih.gov)
  • Tpo-induced adhesion was concentration dependent, reached a maximum following 30 min, and appeared to be dependent on adenylate cyclase, and tyrosine kinase activity. (ox.ac.uk)
  • Unless JohnL or anyone else is prepared to discuss and debate things like the details of how lithium affects the cascade of postsynaptic second-messenger events through adenylate-cyclase regulated protein-kinase, including c-FOS directed gene transcription, its measurement by assaying mRNA and the resulting changes in membrane-bound g-proteins, I don't feel any obligation to at this point justify why Jensen's simple "circuit" models are ludicrous. (dr-bob.org)
  • Multiple modes of insulin regulation of lipolysis and regulation of insulin responses by adenylate cyclase regulators. (nature.com)
  • 5-Methoxytryptamine (1 mM, Calbiochem) caused a 4-fold increase in motility similar to 5HT but had a much diminished effect on cyclic AMP and AC acitivity, while tryptamine (Calbiochem), 5 - hydroxydimethyltryptamine (Sigma-Chem. (erowid.org)
  • Protein kinase activity was 2-5 times greater in the 'head' than in the posterior end of the fluke and in both particulate and supernatant fractions half-maximal activation was obtained at 0.1-0.4 mcM cyclic AMP. (erowid.org)
  • Incubation of fluke heads for 5 min with 1 mM 5HT increased protein kinase activity of both fractions in the absence of added cyclic AMP, but with 5 mcM cyclic AMP protein kinase activity was increased inthe particulate and decreased in the super-natant fraction. (erowid.org)
  • Cyclic AMP increased phosphorylation of fluke protein in both fractions. (erowid.org)
  • At a concentration of 1 mg. per milliliter, tolbutamide also inhibited lipolysis stimulated by ACTH or dibutyryl cyclic AMP. (diabetesjournals.org)
  • Activator of protein kinase A (cyclic AMP agonist). (biolog.de)
  • The phospho-myosin light chain (p-MLC), phospho-myosin light chain kinase (p-MLCK), and phospho-myosin phosphatase target subunit 1 (p-MYPT1) levels in the bladder were examined using Western blot. (hindawi.com)
  • The function of striated or smooth muscle is regulated by the balance of myosin light chain kinase (MLCK) and MLCP activity. (hindawi.com)
  • This gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. (wikipedia.org)
  • Its clinical presentation clearly differs from other forms of GSD, because it is caused by the deficiency of the lysosomal enzyme, alpha-1,4-glucosidase, leading to the pathologic accumulation of normally structured glycogen within the lysosomes of most tissues, differs Three forms of the disease exist: infantile-onset, late-onset juvenile and adult onset. (medscape.com)
  • Background/Aim: Microsomal prostaglandin E synthase-1 (mPGES-1) is an enzyme, which catalyzes the final step of prostaglandin E 2 (PGE 2 ) synthesis. (iiarjournals.org)
  • Microsomal PGE synthase-1 (mPGES-1) is an inducible enzyme that specifically catalyzes the synthesis of PGE 2 in inflammatory conditions. (iiarjournals.org)
  • A new subfamily of short bacterial adenylate kinases with the Mycobacterium tuberculosis enzyme as a model: a predictive and experimental study. (rhea-db.org)
  • A sequence comparison with 52 different forms of adenylate kinases (AK) suggests that the enzyme from M. tuberculosis belongs to a new subfamily of 'short' bacterial AKs. (rhea-db.org)
  • Also displays broad nucleoside diphosphate kinase activity. (enquirebio.com)
  • The increase in 36Cl- permeability is diminished by protein kinase A inhibitors and is not mediated by an increase in intracellular calcium concentrations. (nih.gov)
  • Furthermore, second messenger inhibitors implicated essential and complimentary roles of phosphatidylinositol-3-kinase and protein kinase C in mediating Tpo-induced adhesion. (ox.ac.uk)
  • Concomitant activation of the insulin-like growth factor-1 receptor, Akt, and extracellular signal-regulated protein kinase 1 and 2 as well as increased phosphorylation of Bad also were unique to the hippocampus of APP Sw mice. (jneurosci.org)
  • It is worth noting that we have found the key upstream target of DOX-induced HF, PTP1B, which inhibits the expression of HIF-1α by inhibiting the phosphorylation of IRS, leading to disorders of fatty acid metabolism and glycolysis, which together with the decrease of Nrf2, SOD, Cytc, and AK4 proteins lead to oxidative stress. (frontiersin.org)
  • We measured this using an improved fluorescent reporter and by phosphorylation of endogenous cAMP-response-element binding protein (CREB). (nature.com)
  • Egan, J. J., Greenberg, A. S., Chang, M. K. & Londos, C. Control of endogenous phosphorylation of the major cAMP-dependent protein kinase substrate in adipocytes by insulin and beta-adrenergic stimulation. (nature.com)
  • Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. (origene.com)
  • [ 1 ] In 1952, Cori and Cori demonstrated that glucose-6-phosphatase (G6Pase) deficiency was a cause of GSD type I. (medscape.com)
  • Human adenylate kinase 2 deficiency causes a profound hematopoietic defect associated with sensorineural deafness. (lu.se)
  • The recombinant protein, overexpressed in Escherichia coli, exhibits a low catalytic activity and an unexpectedly high thermal stability (Tm = 64.8 degrees C). Based on various spectroscopic data, on the known three-dimensional structure of the AK from E. coli and on secondary structure predictions for various sequenced AKs, we propose a structural model for AK from M. tuberculosis (AKmt). (rhea-db.org)
  • In addition, it is a rapidly diffusible cytoplasmic protein that specifically reports net PKA activity in situ. (harvard.edu)
  • How hDM2 activity by association with ribosomal proteins is regulated at molecular level remains elusive. (cnrs.fr)
  • In the present study we demonstrate that Tpo can stimulate the adhesion of the Mo7e progenitor cell line to fibronectin (Fn) as well as vascular cell adhesion molecule-1 through activation of very late antigen (VLA)-4 and VLA-5, adhesion molecules previously demonstrated to be involved in regulation of steady state hemopoiesis. (ox.ac.uk)
  • We aim to contribute to the understanding of the regulation of p53 by ribosomal proteins and ribosome assembly factors and build on our previous results to propose molecules which impact the RB/p53 pathway. (cnrs.fr)
  • Differences in expression profiles, substrate specificities, kinetic properties and subcellular localization among the AK (adenylate kinase) isoenzymes have been shown to be important for maintaining a proper adenine nucleotide composition for many different cell functions. (rhea-db.org)
  • d1zunb1 b.43.3.1 (B:238-329) Sulfate adenylate transferase subunit cysN/C, EF-Tu domain 2-like domain {Pseudomonas syringae pv. (berkeley.edu)
  • Tg2576 mice overexpress a mutant form of human amyloid precursor protein with the Swedish mutation (APP Sw ), resulting in high β-amyloid (Aβ) levels in the brain. (jneurosci.org)
  • Briefly, they contain the human APP 695 with the double mutation K670N and M671L (Swedish mutation) and are driven by the prion protein promoter. (jneurosci.org)
  • A DNA sequence encoding the Homo sapiens (Human) AK1 / Adenylate kinase 1, was expressed in the hosts and tags indicated. (enquirebio.com)
  • Thrombopoietin promotes adhesion of primitive human hemopoietic cells to fibronectin and vascular cell adhesion molecule-1: role of activation of very late antigen (VLA)-4 and VLA-5. (ox.ac.uk)
  • Immunohistochemistry of paraffin-embedded human colon using AK1 Polyclonal Antibody at dilution of 1:100 (40x lens). (epigentek.com)
  • Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human AK1 (NP_000467.1). (epigentek.com)
  • The team "RNA Molecular Machines and human pathlogies " specialises in structural biology of proteins and RNA, and of their complexes with small molecules. (cnrs.fr)
  • will use all the techniques of integrative structural biology toolbox to study the human RB/p53 regulon to complete the molecular details of the interaction between hDM2, p53, ribosomal proteins and other ongenic factors. (cnrs.fr)
  • The characterization of human adenylate kinases 7 and 8 demonstrates differences in kinetic parameters and structural organization among the family of adenylate kinase isoenzymes. (rhea-db.org)
  • LysR substrate binding domain, Bacterial regulatory helix-turn-helix protein [Interproscan]. (ntu.edu.sg)
  • SCOPe: Structural Classification of Proteins - extended. (berkeley.edu)
  • Although functional protein conformational change trajectories are to a degree stochastic, they nonetheless pass through a well-defined transition state whose detailed structural properties can rapidly be identified using PATH. (aip.org)
  • Adenylate Kinase is a 3-domain protein that undergoes a large structural rearrangement upon ligand binding. (smog-server.org)
  • During the last we have contributed to the understanding of RNP biogenesis using structural biology to determine the structure of proteins involved in these processes. (cnrs.fr)
  • Proteasomal degradation induced by DPP9‐mediated processing competes with mitochondrial protein import. (uni-koeln.de)
  • Neutralizing Tregs with antibodies (Abs) against CD25 or folate receptor 4 (FR4) inhibited the Foxp3+ Treg angiogenesis and accumulation in WT mice, but not in mPGES-1 −/− mice. (iiarjournals.org)
  • In this work, the free-energy perturbation method was applied to the calculation of relative hydration free energies, relative binding free energies of a ligand-receptor system, and in silico alanine scanning of a peptide-protein complex. (tum.de)
  • The expression of transthyretin, a protein shown to sequester Aβ and prevent amyloid fibril formation in vitro , and several genes in the insulin-signaling pathway, e.g., insulin-like growth factor-2, were increased selectively in the hippocampus of APP Sw mice. (jneurosci.org)
  • The Fap7 ribosome assembly factor is involved in regulates ribosomal RNA processing and is essential for embryogenesis and tumour growth, most probably through the ribosomal protein-hDM2-p53 pathway. (cnrs.fr)
  • Results: Angiogenesis was suppressed in mPGES-1 −/− mice compared with WT mice, which was associated with attenuated forkhead box P3 (Foxp3) expression and Foxp3 + Treg accumulation. (iiarjournals.org)
  • The codon table used was the FreqB table from " Design Parameters to Control Synthetic Gene Expression in Escherichia coli ", which was used because it was shown to express proteins well and was recommended to us by JCVI. (freegenes.org)
  • Our results indicated that 1) The expression of Myogenic factor 6 (Myf6) gene in sfx mice is increased in both female and male mice, while the increase in male mice is much higher than that in female mice. (biomedcentral.com)
  • The expression of creatine kinase muscle type (Ckb) and Cdc42 binding protein kinase beta (Cdc42bpb), on the other hand, although at a low level are increased in both sfx and wild type mice. (biomedcentral.com)
  • Feliciello, A., Rubin, C. S., Avvedimento, E. V. & Gottesman, M. E. Expression of a kinase anchor protein 121 is regulated by hormones in thyroid and testicular germ cells. (nature.com)
  • A group of researchers from the University of Pau (France) developed an ICP-MS-assisted proteomics approach for the characterization of Se-containing proteins in selenium-rich yeast. (speciation.net)
  • We are open to collaborations where we are implicated at various levels: protein cloning, production and purification, binding studies and/or structure solution of the protein of interest in complex with small molecular compounds. (cnrs.fr)
  • Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. (origene.com)
  • Computational treatments of protein conformational changes tend to focus on the trajectories themselves, despite the fact that it is the transition state structures that contain information about the barriers that impose multi-state behavior. (aip.org)
  • The epidemiological association between 7 non-married non-commercial heterosexual partners and case 2 (56-year-old male farmer), 3 cases confirmed by epidemiological investigation and molecular transmission cluster results, 3 cases confirmed by molecular transmission cluster and epidemiological investigation results, and 1 case confirmed by epidemiological investigation results. (bvsalud.org)
  • These RNPs are involved in a wide range of cellular and biochemical functions, from maturation of different classes of RNAs to protein synthesis, and represent some of the most complicated molecular machines. (cnrs.fr)
  • Research, however, reveals that: 1) very small amounts of Al are needed to produce neurotoxicity and this criterion is satisfied through dietary Al intake, 2) Al sequesters different transport mechanisms to actively traverse brain barriers, 3) incremental acquisition of small amounts of Al over a lifetime favors its selective accumulation in brain tissues, and 4) since 1911, experimental evidence has repeatedly demonstrated that chronic Al intoxication reproduces neuropathological hallmarks of AD. (iospress.com)
  • Translation initiation factor 1A / IF-1 [Interproscan]. (ntu.edu.sg)
  • The extraction and following purification were optimized for highest selenium and protein extraction yield. (speciation.net)
  • The success of the approach developed was critically dependent on protein extraction yield and purification quality, and the reproducibility of gel electrophoresis and nanoHPLC separations. (speciation.net)
  • Recent single-molecule experiments pointed out that while double-stranded DNA and RNA differ only slightly in terms of their bending, twisting and stretching deformabilities, they exhibit a qualitative different twist-stretch coupling: Upon overwinding, dsDNA increases, whereas dsRNA decreases its helical extension [1]. (tum.de)
  • Since the EM density only includes 1 molecule, remove 1 copy from the file (for example 4AKE.single.pdb ). (smog-server.org)
  • Search proteins in UniProtKB for this molecule. (rhea-db.org)
  • 28 High-energy configurations, or conformational transition states, therefore impose discrete multi-state behavior on proteins, 21 significantly enhancing function by creating the capacity for a protein to transmit time and ligand-dependent information and/or mechanical motion necessary for signaling and other free-energy transduction processes. (aip.org)
  • For instance, familial AD is linked to mutations in the amyloid precursor protein (APP), presenilin 1, and presenilin 2, all of which lead to increased levels of Aβ. (jneurosci.org)
  • These data indicate that endogenous levels of deltaF508 protein can be stimulated to increase 36Cl- permeability in airway epithelial cells. (nih.gov)
  • We then determined whether an antisense directed at the Aβ region of the amyloid-β protein precursor (AβPP) and previously shown to decrease brain levels of AβPP and to reverse the cognitive impairments of the SAMP8 mouse was able to reverse these impairments. (iospress.com)