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Adenoviruses, HumanAdenovirus E1A ProteinsAdenovirus E4 ProteinsAdenovirus E1B ProteinsAdenovirus Early ProteinsAdenovirus E3 ProteinsAdenoviridaeAdenovirus Infections, HumanAdenoviridae InfectionsAdenovirus E2 ProteinsAdenovirus E1 ProteinsCell Transformation, NeoplasticOncogene Proteins, ViralGenetic VectorsCell Transformation, ViralTransformation, GeneticAdenoviruses, CanineCell LineHeLa CellsMastadenovirusDNA, ViralMolecular Sequence DataBase SequenceTransformation, BacterialGenes, ViralAdenoviruses, PorcineGenetic TherapyAviadenovirusTranscription, GeneticGene Transfer TechniquesFowl adenovirus ATransfectionPromoter Regions, GeneticVirus ReplicationDNA-Binding ProteinsTranscription FactorsViral ProteinsCapsid ProteinsMutationPlasmidsAntigens, Viral, TumorGene Expression Regulation, ViralAmino Acid SequenceCell Line, TransformedE1A-Associated p300 ProteinReceptors, VirusRetinoblastoma ProteinOncolytic VirotherapyTransduction, GeneticOncolytic VirusesNuclear ProteinsSimian virus 40Cells, CulturedKeratoconjunctivitisTumor Cells, CulturedGene Expression RegulationRNA, MessengerRecombinant Fusion ProteinsTranscription Factor DP1Transcriptional ActivationE2F Transcription FactorsRetinoblastoma-Binding Protein 1OncogenesProtein BindingGene ExpressionTumor Suppressor Protein p53Conjunctivitis, ViralAntigens, Polyomavirus TransformingCell NucleusDNA ReplicationTrans-ActivatorsBinding SitesTransgenesAdenovirus VaccinesCapsidRNA, ViralApoptosisFibroblastsMice, NudeCloning, MolecularCell DivisionHelper VirusesDefective VirusesCell Line, TumorCytopathogenic Effect, ViralCricetinae3T3 CellsPolymerase Chain ReactionDNA, RecombinantRecombinant ProteinsChloramphenicol O-AcetyltransferaseRepressor ProteinsKB Cellsbeta-GalactosidasePapillomavirus E7 ProteinsDNAMice, Inbred BALB CProto-Oncogene ProteinsActivating Transcription FactorsDependovirus
ViralRetinoblastoma gene productGeneEpithelialGenesTranscriptional19961989ImmunogenicGenomeCellularOncogenicHistoneCarcinogenesisConstitutivelyBindHumansVariantsHomeoboxEvolutionarily conservedHerpesVaccineHumanCellsPhenotypesTranscriptionKinasesAbnormalActivatorCulturesInfectionSequenceCharacterizationMorphologyPeripheralRoleActivelyTypeActivityMolecularPrimaryFunctionsDomains
Viral8
  • Among Harlow's discoveries was the demonstration that the retinoblastoma protein interacts with viral transforming proteins, thereby linking tumor viruses with the cell cycle. (wikipedia.org)
  • Adenoviral DNA is retained by peripheral epithelial cells of the lung (PLECs) in humans in whom the viral E1A gene is expressed 4 without replication of a complete virus 5 . (ersjournals.com)
  • Of interest is the role of adenoviruses as viral vectors in vaccination and in gene therapy.For example, in viral vector vaccines against SARS-CoV2, the vector virus is used to deliver RNA encoding SARS-CoV2 spike protein into target cells. (medscape.com)
  • The retinoblastoma (Rb) gene product forms a complex with the cellular transcription factor DRTF1, a property assumed to be important for mediating negative growth control because certain viral oncogenes, such as adenovirus E1a, prevent this interaction and mutant Rb alleles, which have lost the capacity to regulate growth, encode proteins that fail to associate with DRTF1. (ox.ac.uk)
  • It also exhaustively introduces the concrete mechanism of invading GC cells and the viral genome composition of adenovirus and herpes simplex virus type 1 (HSV-1). (frontiersin.org)
  • Roles of HTLV-1 basic Zip Factor (HBZ) in Viral Chronicity and Leukemic Transformation. (mdpi.com)
  • Products of the adenovirus E1A gene can act synergistically with cAMP to activate transcription of several viral early genes and the cellular genes c-fos and jun-B. Transcription factor AP-1-binding activity is also induced by the combined action of E1A and cAMP. (princeton.edu)
  • In contrast, the 243-amino acid protein was a poor activator of the viral early genes E2 and E4 compared with the 289-amino acid protein. (princeton.edu)
Retinoblastoma gene product1
Gene11
  • In the present study, the hypothesis that adenoviral infection followed by persistent expression of its E1A gene is capable of producing epithelial-mesenchymal transformation was examined by investigating guinea pig PLECs that express adenoviral E1A. (ersjournals.com)
  • The transforming gene products of these viruses, such as the E1A oncoproteins of adenovirus 5, led to the identification of cellular factor p60, known as cyclin A. This research was the first demonstration of a physical link between cellular transformation and the cell cycle, thereby paving the way for the melding of these two areas of research. (newswise.com)
  • Since the adenoviral E1A gene has been shown to partially transform some epithelial cells from primary rat cell cultures, we constructed retrovirus vectors containing either the 12S or 13S E1A cDNA sequences to facilitate the transfer of these genes into a variety of primary cell types. (cshl.edu)
  • These data show that tumor induction by adenovirus virions is not determined only by the gene products of the transforming region. (uu.nl)
  • These findings implicated the E1A 289 amino acid residue protein of Ad2, a well-known transactivator, as the reactivating function of the endogenous, previously dormant, late E2A promoter-CAT gene assembly. (uni-bielefeld.de)
  • I have previously reported (Frisch, 1991) that adenovirus E1a is a tumor suppressor gene in certain human cell lines. (rupress.org)
  • These data suggest that the 243- and 289-amino acid E1A proteins both interact functionally with the cAMP signaling system to activate transcription of a cellular gene and AP-1-binding activity. (princeton.edu)
  • Mutations in the gene for the antennapedia homeodomain protein are associated with the conversion of antenna to leg or leg to antenna DROSOPHILA. (lookformedical.com)
  • Homeodomain proteins are involved in the control of gene expression during morphogenesis and development (GENE EXPRESSION REGULATION, DEVELOPMENTAL). (lookformedical.com)
  • What does this gene/protein do? (cancerindex.org)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
Epithelial12
  • Epithelial-mesenchymal transformation is now recognised as an important feature of tissue remodelling. (ersjournals.com)
  • Guinea pig primary peripheral lung epithelial cells (PLECs) transfected with adenovirus E1A (E1A-PLECs) were compared to guinea pig normal lung fibroblasts (NLFs) transfected with E1A (E1A-NLFs). (ersjournals.com)
  • E1A transfection results in the transformation of peripheral lung epithelial cells and normal lung fibroblasts to a phenotype intermediate between that of the two primary cells. (ersjournals.com)
  • Case-control studies have shown that the E1A DNA from group C adenoviruses is present in greater amounts in the lungs of patients with COPD than in controls with normal lung function matched for age, sex and smoking history 5 , and that the excess inflammation in the lungs of smokers with severe emphysema is associated with increased numbers of alveolar epithelial cells expressing E1A 6 . (ersjournals.com)
  • Furthermore, E1A DNA and protein persisted in epithelial cells in the lungs of these animals 9 . (ersjournals.com)
  • Reports from several laboratories suggest that a phenomenon termed epithelial-mesenchymal transformation, in which epithelial cells transform into fibroblasts capable of producing connective tissue matrix, is an important feature of tissue remodelling 10 , 11 . (ersjournals.com)
  • The changes that occur during adenoviral E1A transformation of primary guinea pig PLECs were also compared with those occurring during the transformation of the fibroblasts that frequently contaminate primary epithelial cultures, and evidence is presented that both cell types are transformed to an intermediate phenotype. (ersjournals.com)
  • The first is lytic infection, which occurs when an adenovirus enters human epithelial cells and continues through an entire replication cycle, which results in cytolysis, cytokine production, and induction of host inflammatory response. (medscape.com)
  • The 12S E1A virus induced proliferation and immortalization of epithelial cells in rat kidney, liver, heart, pancreas, and thyroid primary cultures. (cshl.edu)
  • 53:742-750, 1985), in which the 12S E1A protein is required for the complete induction of the cellular DNA replication machinery in the quiescent human epithelial cells in which adenoviruses normally replicate. (cshl.edu)
  • E1a induces the expression of epithelial characteristics. (rupress.org)
  • E1a thus appeared to partially convert diverse human tumor cells into an epithelial phenotype. (rupress.org)
Genes5
  • Since E1A and E1B jointly had a more pronounced effect, it was conceivable that genes in both regions acted together in the abrogation of the inhibitory effect of promoter methylations in the late E2A promoter. (uni-bielefeld.de)
  • Such changes are mitigated through rapid expression of the relevant genes for extracellular matrix proteins and growth factors. (westminster.ac.uk)
  • Proteins encoded by homeobox genes (GENES, HOMEOBOX) that exhibit structural similarity to certain prokaryotic and eukaryotic DNA-binding proteins. (lookformedical.com)
  • Their sequences contain a 180 nucleotide sequence designated the homeobox, so called because mutations of these genes often results in homeotic transformations, in which one body structure replaces another. (lookformedical.com)
  • The proteins encoded by homeobox genes are called HOMEODOMAIN PROTEINS. (lookformedical.com)
Transcriptional3
  • The Rb protein therefore acts in trans to repress the transcriptional activity of DRTF1 whereas adenovirus E1a prevents this interaction and thus maintains DRTF1 in a constitutively active state. (ox.ac.uk)
  • Transcriptional repression mediated by the Rb protein and inactivation of repression by the E1a protein are likely to play an important role in mediating their biological effects. (ox.ac.uk)
  • A ubiquitously expressed sequence-specific transcriptional repressor that is normally the target of signaling by NOTCH PROTEINS. (harvard.edu)
19962
  • Adenovirus has been associated with both sporadic and epidemic disease and, with regard to infections among military recruits, who were routinely immunized against types 4 and 7 from 1971 until the cessation of vaccine production in 1996. (medscape.com)
  • The 32-aa MYND domain (for myeloid, Nervy, and Deaf-1) contains non-DNA-binding zinc fingers that are thought to mediate protein-protein interactions (Gross, 1996). (sdbonline.org)
19891
Immunogenic2
  • Adenoviruses are immunogenic and elicit strong innate and adaptive immune responses. (medscape.com)
  • These peptides were then tested in our previously described oncolytic cancer vaccine platform PeptiCRAd, a vaccine platform that combines an immunogenic oncolytic adenovirus (OAd) coated with tumor antigen peptides. (elifesciences.org)
Genome1
  • The genome of adenovirus is well known and can be modified with relative ease to induce lysis or cytotoxicity of a specified cell line without affecting others. (medscape.com)
Cellular2
Oncogenic5
  • Adenovirus is known to be oncogenic in rodents but not in humans. (medscape.com)
  • We have constructed a nondefective recombinant virus between the nononcogenic adenovirus 5 (Ad5) and the highly oncogenic Ad12. (uu.nl)
  • Thus, despite the fact that all products required for oncogenic transformation in vitro were derived from the highly oncogenic Ad12, the recombinant virus did not produce tumors in vivo. (uu.nl)
  • HPV is present in cervical cancer, where it expresses the oncogenic proteins E6 and E7 that inactivate the host regulatory proteins p53 and RB, respectively. (sv40.org)
  • SV40 also expresses the oncogenic protein T Ag in human mesotheliomas, which inactivates p53 and RB. (sv40.org)
Histone1
  • BACKGROUND The histone methyltransferase (HMT) family includes histone lysine methyltransferases (HKMTs) and histone/protein arginine methyltransferases (PRMTs). (cancerindex.org)
Carcinogenesis1
  • These models are being used to systematically elucidate proteins required for cutaneous carcinogenesis and to test their potential role as therapeutic targets. (stanford.edu)
Constitutively1
  • It was demonstrated in transient expression experiments that the 5' CCGG 3'-methylated late E2A promoter was at least partly reactivated in cell lines constitutively expressing the E1 region of Ad2 or of adenovirus type 5 (Ad5) DNA. (uni-bielefeld.de)
Bind2
Humans1
  • Here, we characterize such a protein, iASPP (inhibitory member of the ASPP family), encoded by PPP1R13L in humans and ape-1 in Caenorhabditis elegans. (ox.ac.uk)
Variants1
  • Mouse S49 cells were infected with adenovirus variants expressing either the 243- or 289-amino acid E1A protein and treated with the cAMP analog dibutyryl-cAMP. (princeton.edu)
Homeobox2
  • Antennapedia homeodomain protein is a homeobox protein involved in limb patterning in ARTHROPODS. (lookformedical.com)
  • also known as ASH1), neurogenic differentiation factor 1 (NeuroD1), yes-associated protein 1 (YAP1) and POU class 2 homeobox 3 (POU2F3). (cancerindex.org)
Evolutionarily conserved1
  • Homeodomain-interacting protein kinases (HIPKs) are an evolutionarily conserved kinase family comprising the members HIPK1, HIPK2, and HIPK3 in vertebrates, and, additionally, HIPK4 in mammals. (nature.com)
Herpes1
Vaccine1
  • A live oral vaccine against adenovirus types 4 and 7 was approved for use in this population by the US Food and Drug Administration (FDA) in 2011, and subsequent incidence of acute respiratory disease declined. (medscape.com)
Human3
  • A family of human cdc2-related protein kinases. (wikipedia.org)
  • These human observations are supported by experiments in guinea pigs, where, in the absence of actively replicating virus but the presence of E1A DNA, adenovirus infection resulted in excess lung inflammation following a single exposure to cigarette smoke 7 , and increased parenchymal and airway wall inflammation with greater emphysematous destruction after chronic exposure 8 . (ersjournals.com)
  • An extremely hardy virus, adenovirus is ubiquitous in human and animal populations, survives long periods outside a host, and is endemic throughout the year. (medscape.com)
Cells5
  • Upon infection with adenovirus, one of three different interactions with the cells may occur. (medscape.com)
  • In the two cases tested, heart and liver cultures, E1A-immortalized cells were nontumorigenic, but could be completely transformed by subsequent introduction of the ras oncogene. (cshl.edu)
  • Hence, an inhibitory form of ASPP resembling 53BP2 could allow cells to bypass the tumor-suppressor functions of p53 and the ASPP proteins. (ox.ac.uk)
  • Moreover, iASPP is an oncoprotein that cooperates with Ras, E1A and E7, but not mutant p53, to transform cells in vitro. (ox.ac.uk)
  • Significant E1A-dependent induction of c-fos mRNA and AP-1-binding activity was observed in cells expressing either E1A protein. (princeton.edu)
Phenotypes3
  • Homeotic mutations in Drosophila can result in dramatic phenotypes that suggest the possibility for rapid morphological evolution, but dissection of the genetic pathway downstream of Ultrabithorax is beginning to reveal how wing morphology may have evolved by more gradual transformations. (lookformedical.com)
  • Several other genetic backgrounds result in enlargement of the haltere significantly beyond the normal range of haploinsufficient phenotypes, suggesting genetic variation in cofactors that mediate homeotic protein function. (lookformedical.com)
  • Introgression of Antennapedia produces lines with heritable phenotypes ranging from almost complete suppression to perfect antennal leg formation, as well as transformations that are restricted to either the distal or proximal portion of the appendage. (lookformedical.com)
Transcription2
  • In this study, we show that the wild-type Rb protein can specifically repress transcription from promoters driven by DRTF1 whereas a naturally occurring mutant Rb protein cannot. (ox.ac.uk)
  • Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process. (lookformedical.com)
Kinases1
  • Homeodomain-interacting protein kinases (HIPKs) belong to the CMGC kinase family and are closely related to dual-specificity tyrosine phosphorylation-regulated kinases (DYRKs). (nature.com)
Abnormal1
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
Activator1
  • Electrophoretic mobility shift assays were performed in order to examine nuclear factor (NF)-κB and activator protein (AP)-1 binding activities. (ersjournals.com)
Cultures2
  • Adenovirus, a DNA virus, was first isolated in the 1950s in adenoid tissue-derived cell cultures, hence the name. (medscape.com)
  • These primary cell cultures were often noted to spontaneously degenerate over time, and adenoviruses are now known to be a common cause of asymptomatic respiratory tract infection that produces in vitro cytolysis in these tissues. (medscape.com)
Infection3
  • The present report concerns the role of adenovirus infection in inducing this transformation in an animal model of chronic obstructive pulmonary disease. (ersjournals.com)
  • Adenovirus is often cultured from the pharynx and stool of asymptomatic children, and most adults have measurable titers of anti-adenovirus antibodies, implying prior infection. (medscape.com)
  • Recovery from adenovirus infection is associated with the development of serotype-specific neutralizing antibodies. (medscape.com)
Sequence4
  • The inactivating effect of sequence-specific promoter methylations was extensively studied by using the late E2A promoter of adenovirus type 2 (Ad2) DNA. (uni-bielefeld.de)
  • Immunoglobulin J Recombination Signal Sequence-Binding Protein" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
  • This graph shows the total number of publications written about "Immunoglobulin J Recombination Signal Sequence-Binding Protein" by people in Harvard Catalyst Profiles by year, and whether "Immunoglobulin J Recombination Signal Sequence-Binding Protein" was a major or minor topic of these publication. (harvard.edu)
  • Below are the most recent publications written about "Immunoglobulin J Recombination Signal Sequence-Binding Protein" by people in Profiles. (harvard.edu)
Characterization1
  • Development and further characterization of a small subclass of rat olfactory receptor neurons that shows immunoreactivity for the HSP70 heat shock protein. (morimotolab.org)
Morphology1
  • Both exhibited cuboidal morphology and junctional complexes, but did not contain lamellar bodies or express surfactant protein A, B or C mRNAs. (ersjournals.com)
Peripheral1
  • efficient and reliable establishment of lymphoblastoid cell lines by epstein-barr virus transformation from a limited amount of peripheral blood. (liverpool.ac.uk)
Role1
  • It is postulated that this intermediate phenotype may play a major role in the remodelling of the airways in chronic obstructive pulmonary disease associated with persistence of adenovirus E1A DNA. (ersjournals.com)
Actively1
  • yet, a global view of bcr activation-regulated protein ubiquitination is lacking when ebv is actively replicating. (liverpool.ac.uk)
Type1
  • A composite interval mapping procedure was used to demonstrate that one major effect locus accounts for three-quarters of the variance for haltere to wing margin transformation in Ultrabithorax flies, yet has no obvious effect on wild-type development. (lookformedical.com)
Activity1
  • The Rb protein and adenovirus E1a therefore have opposite effects on the activity of a common molecular target. (ox.ac.uk)
Molecular2
  • the discovery of the molecular machinery of autophagy, namely atg proteins, was awarded with the nobel prize in physiology and medicine to yoshinori ohsumi in 2016. (liverpool.ac.uk)
  • Molecular Chaperone Machines: Chaperone Activities of the Cyclophilin Cyp-40 and the Steroid Aporeceptor-Associated Protein P23. (morimotolab.org)
Primary1
Functions1
  • It will now be demonstrated that the inactivation of the late promoter of Ad2 DNA can be reversed by transactivating functions that are encoded in the 13S messenger RNA of the E1A region of Ad2 DNA. (uni-bielefeld.de)
Domains1
  • The 576-aa DEAF-1 protein possesses two conserved domains, SAND and MYND. (sdbonline.org)