Adenovirus genes. Medical search. Frequent questions

In this review article, we outline key characteristics of major gene therapy viruses-adenovirus, adeno-associated virus (AAV), and lentivirus-and summarize the mechanisms regulating important steps in the virus journey from binding at peripheral nerve terminals to nuclear delivery. (frontiersin.org)
Method: Adenovirus (AdV), Adeno-Associated Virus (AAV), Baculovirus (BV) and Lentivirus (LV) vectors encoding Green Fluorescent Protein (GFP) were injected bilaterally intravitreally into adult C57BL/6OlaHsd mice. (eurekaselect.com)
Several vectors offer clinically relevant options including adenovirus (AV), adeno-associated virus (AAV) and lentivirus. (medgadget.com)

In this study, we have examined the feasibility of gene transfer to human corneal endothelium, using a number of recombinant adenovirus constructs. (ox.ac.uk)
BACKGROUND: Immunization of BALB/c mice with a recombinant adenovirus expressing Mycobacterium tuberculosis (M. tuberculosis) antigen 85A (Ad85A) protects against aerosol challenge with M. tuberculosis only when it is administered intra-nasally (i.n. (ox.ac.uk)
In the present study we characterized the recombinant adenovirus vector AdYB-1 that expresses the human transcription factor YB-1 under CMV promotor control. (tum.de)

Of interest is the role of adenoviruses as viral vectors in vaccination and in gene therapy.For example, in viral vector vaccines against SARS-CoV2, the vector virus is used to deliver RNA encoding SARS-CoV2 spike protein into target cells. (medscape.com)
Suicide gene systems that convert nontoxic systemically delivered prodrugs to active chemotherapeutic agents have been delivered via adenoviral vectors directly into cancer cells. (medscape.com)
The complex mechanisms by which viral vectors may be incorporated into gene therapy and the rapid growth in this field put further discussion beyond the scope of this text. (medscape.com)
Ex vivo infection of human corneas with adenoviral vectors containing lacZ, under transcriptional control of either cytomegalovirus (CMV) or Rous sarcoma virus (RSV) promoters, provided high-level gene expression, which was largely restricted to endothelium. (ox.ac.uk)
The functionality of the IL-12 or CD gene products expressed from these vectors was validated by splenic interferon (IFN)-γ production or viability assays in cultured cells. (biomedcentral.com)
Therapeutic gene transfer using viral or nonviral vectors has been shown to be clinically feasible and safe. (biomedcentral.com)
Conclusion: Our findings show that intravitreal gene delivery is safe and feasible with AAV, AdV and lentivirus vectors. (eurekaselect.com)
Adenoviruses are widely used as experimental gene therapy vectors as they are capable of delivering a large genetic payload and are able to target a wide range of cell-types. (gla.ac.uk)
US officials immediately began notifying the 100 or so gene therapy experimenters using adenovirus vectors, which are made using a disarmed version of the virus that causes the common cold. (nature.com)
Verma describes vectors as the "Achilles heel" of gene therapy, and says that dose-escalation studies using adenoviruses should be re-examined. (nature.com)
Most gene therapy involves retroviral vectors, but adenoviruses are popular for cancer and cystic fibrosis. (nature.com)
Verma says he thinks vectors like the one used in the OTC trial will soon be abandoned in favour of 'gutless' adenoviruses, retroviruses, AAV or lentivectors. (nature.com)
Dr. Wilson's laboratory focuses on the development of gene transfer vectors and their application in the treatment of inherited and acquired diseases. (upenn.edu)
In a career spanning four decades, he has identified, isolated, and developed virus-based vectors for in vivo gene therapy. (upenn.edu)
His laboratory is responsible for discovering a new family of endogenous adeno-associated viruses (AAV) that have become best-in-class for in vivo gene therapy vectors. (upenn.edu)
A robust, discovery-oriented research program in virology, vector engineering, and gene editing complements the translational portfolio and informs the development of next generation vectors and therapies. (upenn.edu)
Novel adeno-associated viruses from rhesus monkeys as vectors for human gene therapy. (upenn.edu)

Adenovirus E1B protein usually refers to one of two proteins transcribed from the E1B gene of the adenovirus: a 55kDa protein and a 19kDa protein. (wikipedia.org)
E1B proteins work to prevent apoptosis that is induced by the small adenovirus E1A protein, which stabilizes p53, a tumor suppressor. (wikipedia.org)
Ex vivo adenovirus-mediated gene transfer and immunomodulatory protein production in human cornea. (ox.ac.uk)
Subsequently, we examined the expression of a potential therapeutic gene, CTLA-4 Ig fusion protein. (ox.ac.uk)
Recombinant adenoviruses expressing green fluorescent protein (Ad-GFP), rat relaxin (Ad-rRLN) and mouse relaxin (Ad-mRLN) were generated and Ad-rRLN and Ad-mRLN were demonstrated to direct the expression of bioactive relaxin peptides in vitro. (nih.gov)
In conclusion, we provide evidence that early virus-mediated gene transfer of a sarcoglycan protein constitutes a promising therapeutic strategy for LGMDs and that the benefits of this approach can easily and effectively be monitored by noninvasive methodologies such as MRI. (ncl.ac.uk)
The Rb protein therefore acts in trans to repress the transcriptional activity of DRTF1 whereas adenovirus E1a prevents this interaction and thus maintains DRTF1 in a constitutively active state. (ox.ac.uk)
The Rb protein and adenovirus E1a therefore have opposite effects on the activity of a common molecular target. (ox.ac.uk)
Once the viruses and their gene cargoes infected liver cells, the infected cells expressed the gene and produced iRFP protein. (sciencedaily.com)
Upon intravenous delivery however, many adenoviruses become coated in the blood coagulation protein Factor X (FX). (gla.ac.uk)
We solved the structure of adenovirus type 5 decorated with FX, showing that it binds to the major capsid protein known as hexon. (gla.ac.uk)
We measured the levels of gene 5 and gene 6 mRNA and showed that they were not significantly different, and protein analysis indicated no difference in stability of NSP1 compared with VP6. (microbiologyresearch.org)
Nucleotide sequence and expression in Escherichia coli of the gene encoding the nonstructural protein NCVP2 of bovine rotavirus. (microbiologyresearch.org)
Cloning and nucleotide sequence of the simian rotavirus gene 6 that codes for the major inner capsid protein. (microbiologyresearch.org)
The adenovirus-vectored covid vaccines all contain DNA from the engineered spike protein. (naturalnews.com)
The adenovirus-vectored vaccines carry the spike protein DNA into the cell by using the adenovirus to subvert the innate immune response. (naturalnews.com)
NIP3 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. (thermofisher.com)
Hexon is the most abundant protein in the icosahedral capsid, and the hexon gene is commonly analyzed to characterize and determine adenovirus types ( 5 ). (cdc.gov)
To best fit the sequence data for the hexon gene, we used the DNA/Protein model test to identify the optimal evolutionary model, GTR+Γ+I (general time reversible + gamma + invariable). (cdc.gov)
But under the guidance and expertise of Dr Colin Dinney at MD Anderson - who is really the person to be credited with the development of nadofaragene, which has been a passion and a brainchild of his - we looked at changing from developing the protein as the treatment of choice to developing a gene vector. (medscape.com)
All human infections caused by a new influenza subtype are required to be reported under the International Health Regulations (IHR, 2005).4 This includes any influenza A virus that has demonstrated the capacity to infect a human and its haemagglutinin gene (or protein) is not a mutated form of those, i.e. (who.int)

2 recent reports have indicated probable human infection with simian adenoviruses (SAdVs). (cdc.gov)
Genes within the E3 transcription unit of human adenoviruses modulate host immune responses to infection . (bvsalud.org)
These primary cell cultures were often noted to spontaneously degenerate over time, and adenoviruses are now known to be a common cause of asymptomatic respiratory tract infection that produces in vitro cytolysis in these tissues. (medscape.com)
Adenovirus is often cultured from the pharynx and stool of asymptomatic children, and most adults have measurable titers of anti-adenovirus antibodies, implying prior infection. (medscape.com)
Recovery from adenovirus infection is associated with the development of serotype-specific neutralizing antibodies. (medscape.com)
Upon infection with adenovirus, one of three different interactions with the cells may occur. (medscape.com)
The first is lytic infection, which occurs when an adenovirus enters human epithelial cells and continues through an entire replication cycle, which results in cytolysis, cytokine production, and induction of host inflammatory response. (medscape.com)
NSP1 (gene 5) and VP6 (gene 6) are expressed early in infection, and VP6 is expressed in excess over NSP1. (microbiologyresearch.org)
In contrast to the respiratory serotype Ad5, the preferential ocular tropism of Ad19 and subsequent productive infection likely depends both on its control of some host differentiation/development genes and on the different host defense /inflammatory responses it elicits. (arvojournals.org)
Human adenovirus (Adv) infection is responsible for most community-acquired pneumonia in infants and children, which results in significant morbidity and mortality in children every year. (hindawi.com)
Knowing the miRNA expression profile will help understand the role of miRNAs in modulating the host response to adenovirus infection and possibly improve the diagnosis of adenovirus-infected pneumonia. (hindawi.com)
Human adenovirus (Adv) infection is responsible for most community-acquired pneumonia in infants and children [ 1 , 2 ]. (hindawi.com)
Also, viral infection has proved to have a great impact on cellular small RNA expression and gene expression [ 9 - 11 ]. (hindawi.com)
CDC is investigating a possible association between pediatric hepatitis and adenovirus infection. (cdc.gov)
The types of specimens you should collect for human adenovirus detection depend on the patient's clinical presentation and type of infection. (cdc.gov)
RÉSUMÉ La flambée d'une infection par le nouveau coronavirus en Arabie saoudite en 2012 a principalement affecté des hommes et des résidents urbains. (who.int)

Adenovirus serotype 14 (Ad14) is a rarely reported but emerging serotype of adenovirus that can cause severe and sometimes fatal respiratory illness in patients of all ages, including healthy young adults. (cdc.gov)

However, the greatest challenge in viral gene therapy, as might be expected, is the immune response to the viral vector itself. (medscape.com)
The objective of the work presented in Chicago was to evaluate CVMR-BAC as an expression vector of foreign genes. (thepoultrysite.com)
CONCLUSIONS: Our microarray analysis represents the first ex vivo study comparing gene expression profiles of CD8 T cells isolated from distinct sites after immunization with an adenoviral vector by different routes. (ox.ac.uk)
Adenovirus vector production and purification. (nih.gov)
Their enquiries centre on the adenovirus vector used to deliver potentially therapeutic DNA to the liver. (nature.com)
According to the protocol, researchers at the University of Pennsylvania's Institute for Human Gene Therapy used an 'E1-deleted, E2A-temperature-sensitive' adenovirus vector to infect liver cells with the normal OTC gene, which codes for a urea-cycle enzyme that removes excess nitrogen from the body. (nature.com)
Using bcl-2-expressing adenovirus vector we investigated the effect of Bcl-2 on UVB-induced apoptosis. (bioone.org)
In this manuscript, we have developed a recombinant human adenovirus as an expression vector that contains the gene for porcine interferon alpha. (usda.gov)
it will be called ZyCoV-D. This vaccine contains less DNA than the other DNA vaccines and that DNA is not concealed by a viral vector (adenovirus). (naturalnews.com)
For example, an adeno-associated AAV2 vector injected with the therapeutic gene (RPE65) into the retina enhanced the eyesight of people with Lebers congenital Amaurosis. (medgadget.com)

The sequence of SAdV-18 was closely related to that of human adenovirus F across the whole genome, and the new isolates were found to harbor 2 fiber genes similar to those of human adenovirus (HAdV) strains HAdV-40 and HAdV-41, which can cause infectious diarrhea. (cdc.gov)
The genome of adenovirus is well known and can be modified with relative ease to induce lysis or cytotoxicity of a specified cell line without affecting others. (medscape.com)
For this purpose, the VP2 gene of infectious bursal disease virus (IBDV) was cloned into CVRM genome by recombination. (thepoultrysite.com)
The objective of work presented by Sanjay M. Reddy of Texas A&M University was to evaluate potential cloning sites in the HVT genome for stable expression of foreign genes. (thepoultrysite.com)
While each DNA blueprint, or gene, encodes for a different machine or structure, the genome, the collection of DNA wrapped up inside the nucleus, is much more active than a quiet library of neatly stacked blue-prints. (birmingham.ac.uk)
We are investigating the impact of specific classes of gene mutations, such as ATM, BRCA1, and MYBL2 on genome integrity. (birmingham.ac.uk)
Adenovirus DNA : the viral genome and its expression / edited by Walter Doerfler. (who.int)

Ad14 isolates from all four states were identical by sequence data from the full hexon and fiber genes. (cdc.gov)

In CaCo-2 colonic epithelial cells, the expression of this gene increases with cell differentiation. (aacrjournals.org)

Since 2011, several novel adenoviruses have been isolated from nonhuman primates, including a group of new strains, designated SAdV-B, from asymptomatic rhesus macaques housed at 5 different primate facilities located across the United States ( 2 , 4 ). (cdc.gov)

Adenoviruses commonly infect vertebrates, including humans and nonhuman primates ( 1 ). (cdc.gov)

The current classification of macaque adenoviruses by the International Committee on Taxonomy of Viruses has 1 defined species, simian adenovirus A (SAdV-A), to which SAdV-3 has been assigned. (cdc.gov)
One simian adenovirus species, SAdV-A, has been recognized by the International Committee on Taxonomy of Viruses ( 2 ). (cdc.gov)
In addition, at least 25 simian adenovirus types have been identified ( 3 ). (cdc.gov)
We previously reported the detection of several new simian adenovirus strains in fecal specimens from captive monkeys in China ( 3 ). (cdc.gov)
Consequently, we further characterized 10 of these novel simian adenovirus strains by sequencing the entire open-reading frames of the hexon genes. (cdc.gov)
We performed alignment analysis of the complete nucleotide hexon sequences of the 10 novel strains and of reference human and simian adenoviruses by using the MUSCLE program within the MEGA5 software package ( 6 ). (cdc.gov)
Hexon genes of the 10 simian adenovirus strains ranged in length from 2,739 to 2,820 nt and from 913 to 940 aa. (cdc.gov)
Comparison of the hexon gene sequences of simian adenovirus types identified in this study with human and simian adenovirus sequences from GenBank revealed 9 previously reported hypervariable regions (HVRs): HVR1-HVR9 ( 5 , 7 ). (cdc.gov)
The simian adenovirus types within the same cluster were closely related in all 9 HVRs (data not shown). (cdc.gov)
Phylogenetic analysis of complete hexon gene nucleotide sequences from human and simian adenoviruses. (cdc.gov)

Postmortem tracheal and gastric swabs from the infant were sent to the Wadsworth Center laboratory of the New York State Department of Health, where adenovirus was detected by polymerase chain reaction (PCR). (cdc.gov)

Additionally, we describe how neuropathology can negatively influence these pathways, and conclude by discussing opportunities to optimize the intramuscular administration route to maximize gene delivery and thus therapeutic potential. (frontiersin.org)
Therapeutic gene transfer affords a clinically feasible and safe approach to cancer treatment but a more effective modality is needed to improve clinical outcomes. (biomedcentral.com)
Combined transfer of therapeutic genes with different modes of actions may be a means to this end. (biomedcentral.com)
However, the therapeutic outcome appears not to be high as expected, presumably because the in vivo gene delivery efficiency is low. (biomedcentral.com)
Combined transfer of genes with different mechanisms of action may potentate the therapeutic outcome. (biomedcentral.com)
Thus, it is important to develop innovative therapeutic strategies such as oncolytic adenoviruses in cancer therapy. (tum.de)

These two proteins are needed to block apoptosis in adenovirus-infected cells. (wikipedia.org)
Homologous recombination was identified in open reading frames for E3 CR1α, CR1ß, and CR1Î³, similar to that previously observed with genes encoding the three major structural capsid proteins , the penton base, hexon, and fiber. (bvsalud.org)
The retinoblastoma (Rb) gene product forms a complex with the cellular transcription factor DRTF1, a property assumed to be important for mediating negative growth control because certain viral oncogenes, such as adenovirus E1a, prevent this interaction and mutant Rb alleles, which have lost the capacity to regulate growth, encode proteins that fail to associate with DRTF1. (ox.ac.uk)
Proteins transcribed from the E1B region of ADENOVIRUSES which are involved in regulation of the levels of early and late viral gene expression. (bvsalud.org)

His research efforts and novel technology have contributed to successful clinical programs of in vivo gene therapy for rare monogenic diseases developed by other companies, including Glybera® for familial lipoprotein lipase deficiency (AAV1), Luxturna® for RPE65-mediated retinal dystrophy (AAV2), and Zolgensma® for spinal muscular atrophy (AAV9). (upenn.edu)

The high prevalence of adenoviruses in fecal samples from asymptomatic rhesus macaques and the similarity of the isolates to human strains indicates the possibility of animal-to-human transmission of SAdVs. (cdc.gov)
To clarify the nature of adenoviruses shed in the feces of asymptomatic monkeys, we attempted to culture adenoviruses from fecal samples from rhesus macaques ( Macaca mulatta ) housed in 5 primate facilities in the United States. (cdc.gov)
They raised concerns about the risks of the treatment and its use-for the first time in gene therapy-in asymptomatic patients. (nature.com)

The classification of monkey adenoviruses was initially done in a manner analogous to that used for human isolates, i.e., on the basis of differences in their ability to agglutinate erythrocytes from different species. (cdc.gov)
Homologous recombination in E3 genes of human adenovirus species D. (bvsalud.org)
A comprehensive genomics and bioinformatics analysis of the E3 transcription unit for 38 viruses within human adenovirus species D (HAdV-D) revealed distinct and surprising patterns of homologous recombination . (bvsalud.org)
A broad-range primer pair was used, targeting a portion of the hexon gene, in combination with species-specific primer pairs targeting a portion of the fiber gene. (unicatt.it)
Quantitative real-time PCR assays for identification of species C human adenoviruses. (cdc.gov)

2 , 3 ) identified adenoviruses as one of several classes of viruses found as outgrowths from primary cultures of monkey kidney cells that were being cultivated for propagation of poliovirus. (cdc.gov)
SAdV-1 and SAdV-7, while not considered human viruses, are now classified as human adenovirus G (HAdV-G) because of their similarity to a virus (HAdV-52) isolated from a human patient with gastroenteritis ( 7 ). (cdc.gov)
Administration of gene therapy viruses into skeletal muscle, where distal terminals of motor and sensory neurons reside, has been shown to result in extensive transduction of cells within the spinal cord, brainstem, and sensory ganglia. (frontiersin.org)
Gene therapy viruses are non-replicating, but still hijack host cell machinery to express transgenes of interest in the nucleus. (frontiersin.org)
This allowed our colleagues in the Institute of Cardiovascular and Medical Sciences to design mutant viruses that do not bind FX and therefore may be used to target efficiently different organs for gene therapy. (gla.ac.uk)
More importantly, recombinant versions of these viruses have proven to be useful as improved gene transfer vehicles to a variety of targets such as the liver, cardiac and skeletal muscle, and the brain. (upenn.edu)

We have previously found that adenovirus DNA is readily detected in fecal samples from monkeys who have no symptoms of any clinical adenoviral disease ( 9 ). (cdc.gov)
This work implies that, to achieve comparable gene expression in the injured liver, higher adenoviral titers may be required, an important consideration as gene therapy in disease states is considered. (duke.edu)

Cells were transduced in vitro with an adenovirus encoding beta-galactosidase (Ad.beta-gal) over a range of viral titers, and transduced cells were identified by detection of X-gal. (duke.edu)

The rat HMGCS2 gene contains elements that control its multihormonal regulation and tissue specificity ( 11 - 14 ). (aacrjournals.org)
We have investigated potential mechanisms of regulation of rotavirus gene expression by functional evaluation of two differentially expressed viral mRNAs. (microbiologyresearch.org)
YB-1 plays an important role in cellular gene expression and regulation and is associated with P-glycoprotein dependent multidrug resistence (MDR). (tum.de)
Regulation of bacterial virulence gene expression by the host environment. (jci.org)

The worldwide Gene Therapy Market is estimated at $ 584 million in 2016 and is expected to reach $ 442 million by 2023, with a CAGR of 33.3% from 2017 to 2023. (medgadget.com)

Further, HAdV-52 ( 7 ) isolated from a patient with gastroenteritis has been found to be very dissimilar to all previously isolated human adenoviruses but closely related to monkey adenoviruses SAdV-1 and SAdV-7. (cdc.gov)

These novel strains were detected by using PCR and hexon gene-specific primers, but the product length (≈255 bp) may be insufficient to completely and accurately characterize these strains. (cdc.gov)

Adenoviruses, which can cause infectious diarrhea or respiratory infections in humans, were isolated from monkeys soon after their initial characterization by Rowe et al. (cdc.gov)
A live oral vaccine against adenovirus types 4 and 7 was approved for use in this population by the US Food and Drug Administration (FDA) in 2011, and subsequent incidence of acute respiratory disease declined. (medscape.com)
Quantitative real-time PCR assay panel for detection and type-specific identification of the epidemic respiratory human adenoviruses. (cdc.gov)

Statistically significant (p ≤ 0.05) differentially expressed genes were analyzed to determine if they mapped to particular molecular functions, biological processes or pathways using Gene Ontology and Panther DB mapping tools. (ox.ac.uk)

Here we report that a single intramuscular injection of a first generation adenovirus into the skeletal muscle of neonate mice led to sustained expression of alpha-sarcoglycan at the sarcolemma of transduced myofibers for at least 7 months. (ncl.ac.uk)

The molecular steps in the replication pathway that regulate the levels of viral gene expression are not well defined. (microbiologyresearch.org)
Molecular typing of human adenovirus by PCR and sequence of a partial region of the hexon gene. (cdc.gov)

Given the close physical proximity and intermingling of human and monkey populations in several locales in Asia, Africa, and Latin America, better characterization of monkey adenoviruses is of public health importance. (cdc.gov)

Adenoviruses are immunogenic and elicit strong innate and adaptive immune responses. (medscape.com)
Most of the target genes were involved in the MAPK signaling pathway and innate immune response. (hindawi.com)

Small RNAs include microRNA (miRNA), siRNA, tRFs, piRNA, and rasiRNAs, which regulate gene expression in a wide range of processes such as viral replication and host immune response. (hindawi.com)
miRNAs regulate gene expression in a wide range of physiological and pathological processes such as in immune response and viral replication [ 18 ]. (hindawi.com)
A Favorable Benefit-Risk Balance Maybe Expected with the Replication-defective Adenovirus-mediated Interferon Gene Therapy for Cancer Treatment. (cdc.gov)

However, in adenovirus-infected cells, activated BAK and BAX are sequestered by E1B-19k, preventing the pathway. (wikipedia.org)
Scholars@Duke publication: Adenovirus-mediated gene transfer to nonparenchymal cells in normal and injured liver. (duke.edu)
Adenovirus-mediated gene transfer has become an important tool with which to introduce genetic material into cells. (duke.edu)
The aim of this study was to evaluate gene transfer to nonparenchymal (and parenchymal) cells of the normal and injured rat liver. (duke.edu)
Chemokine gene expression in lung CD8 T cells correlates with protective immunity in mice immunized intra-nasally with Adenovirus-85A. (ox.ac.uk)
In order to determine why the i.n. route is so effective compared to parenteral immunization, we used microarray analysis to compare gene expression profiles of pulmonary and splenic CD8 T cells after i.n. or intra-dermal (i.d.) immunization. (ox.ac.uk)
RESULTS: CD8 T cells from lungs of i.n. immunized mice expressed a large number of chemokines chemotactic for resting and activated T cells as well as activation and survival genes. (ox.ac.uk)
Lung lymphocytes from i.n. immunized mice also express the chemokine receptor gene Cxcr6, which is thought to aid long-term retention of antigen-responding T cells in the lungs. (ox.ac.uk)
The sustained expression of chemokines and activation genes enables CD8 T cells to remain in the lungs for extended periods after i.n. immunization. (ox.ac.uk)
Sustained expression of the gene is therefore impossible, and the immune system may destroy infected cells-the very cells targeted for help. (nature.com)
Adv infects the host cells through binding to different cellular receptors such as coxsackievirus and adenovirus receptor (CAR) [ 12 ]. (hindawi.com)
PDGF-receptor b -targeted adenovirus redirects gene transfer from hepatocytes to activated stellate cells. (rug.nl)
The incremental difference of this gene therapy over others is it's locally delivered - it's easily delivered by putting a catheter into the bladder, putting the medicine inside the bladder, and then allowing the gene to transfuse into the bladder cells. (medscape.com)

Adenovirus is known to be oncogenic in rodents but not in humans. (medscape.com)

In contrast, control animals inoculated with an adenovirus lacking the interferon gene developed typical signs of FMD. (usda.gov)

Virus-mediated gene therapy has the potential to deliver exogenous genetic material into specific cell types to promote survival and counteract disease. (frontiersin.org)
Exploding the gene myth : how genetic information is produced and manipulated by scientists, physicians, employers, insurance companies, educators, and law enforcers / Ruth Hubbard and Elijah Wald. (who.int)

Adenoviruses were also found in rectal swab and fecal samples taken from monkeys in captivity ( 4 , 5 ). (cdc.gov)

Human adenoviruses (HAdVs) are common pathogens associated with a variety of clinical manifestations. (unicatt.it)
Other recently identified pathogens include the enteric adenoviruses, calicivirus, astrovirus, and the Norwalk family of agents. (cdc.gov)

This route is minimally invasive and therefore clinically relevant for gene therapy targeting to peripheral nerve soma. (frontiersin.org)

This gene provides instructions for making an enzyme called neuraminidase 1 (NEU1), which is found in lysosomes . (medlineplus.gov)
At least 40 disease-causing mutations in the neuraminidase 1 ( NEU1 ) gene have been reported. (medscape.com)

These highly upregulated microRNAs may have crucial roles in Adv pathogenesis and are potential biomarkers for adenovirus-infected pneumonia. (hindawi.com)

In this study, recombinant (r) adenoviruses (Ads) encoding full-length and truncated rHu BChEs were tested for in vivo expression in mice. (nebraska.edu)
We have evaluated the antitumor effect of combining IL-12 with CD gene transfer in mice bearing renal cell carcinoma (Renca) tumors. (biomedcentral.com)
Adenovirus particles containing the gene for iRFP were injected into mice. (sciencedaily.com)

Additional information on adenovirus testing at CDC is available at Submitting Specimens to CDC . (cdc.gov)
Before collecting any specimens, discuss with a laboratory and a clinician familiar with adenovirus. (cdc.gov)
To improve human adenovirus detection, you should collect specimens within a week of symptom onset. (cdc.gov)

In addition, increased government support, increased ethical acceptance of gene therapy for cancer treatment, and increased cancer spread can drive growth in the gene therapy market. (medgadget.com)

North America is the largest contributor to the 2016 gene therapy market. (medgadget.com)
Among the gene type, antigen dominates the overall market accounting for two-ninths of the total share in 2016. (medgadget.com)
In 2016, North America accounted for maximum contribution to the total revenue generated, owing to the high prevalence rate of cancer, presence of high disposable income, and high funding for R&D activities associated with gene therapy. (medgadget.com)

Systematic review of reported association studies of monogenic genes and bladder cancer risk and confirmation analysis in a large population cohort. (cdc.gov)

Human Gene Therapy , 19(4), p. 40. (gla.ac.uk)

Possessing over60 serotypes, adenovirus is recognized as the etiologic agent of various diverse syndromes. (medscape.com)
Adenovirus (Ad) is the major cause of viral conjunctivitis worldwide, with serotypes Ad8, Ad19 and Ad37 predominantly infecting the eye. (arvojournals.org)

From Texas A&M University, Blanca Lupiani reported that she and her colleagues recently showed that insertion of LTR (long term repeat) sequences into the CVI988 resulted in the generation of CVRM, a virus with similar protective efficacy.The CVRM virus was cloned as a bacterial artificial chromosome, by insertion of mini-F sequences into the US2 gene by homologous recombination. (thepoultrysite.com)

Hence a single systemic injection of adenovirus producing mouse relaxin reverses cardiac fibrosis without adversely affecting normal collagen levels in other organs and establishes the potential for the use of relaxin gene therapy for the treatment of cardiac fibrosis. (nih.gov)

With thousands of clinical trials to date, gene therapy is a flourishing strategy with great promise for the treatment of diseases impacting the nervous system. (frontiersin.org)
Through retrospective examination of laboratory reports from the three clinical laboratories in the state that have virology capacity and the Oregon State Public Health Laboratory (OSPHL), OPHD identified 68 persons who tested positive (by culture, PCR, or IFA) for adenovirus during November 1, 2006--April 30, 2007. (cdc.gov)
CLINICAL RELEVANCE Gene therapy with rAd.p21 may provide an effective antiproliferative for glaucoma filtration surgery, without the complications associated with mitomycin. (wisc.edu)

now, however, they are often classified by sequence and phylogenetic analyses of their respective genes ( 2 ). (cdc.gov)

Mutations in the NEU1 gene cause sialidosis. (medlineplus.gov)
Mutations in the NEU1 gene lead to a shortage (deficiency) of the NEU1 enzyme. (medlineplus.gov)
which means both copies of the gene in each cell have mutations. (medlineplus.gov)
Sialidosis should not be confused with disorders of free sialic acid storage, which are caused by a defect in the lysosomal transport of free sialic acid due to mutations in the AST (anion and sugar transporter) gene. (medscape.com)
Spinal muscular atrophies usually result from autosomal recessive mutations that affect the survival motor neuron 1 ( SMN1 ) gene on the long arm of chromosome 5, most often causing a homozygous deletion of exon 7. (msdmanuals.com)

Rapoza classified monkey adenoviruses into 4 hemagglutination subgroups on the basis of their differential agglutination properties with respect to their ability to agglutinate rat, rhesus, and guinea pig erythrocytes ( 6 ). (cdc.gov)
Additional samples were obtained from rhesus macaques housed at the Oregon National Primate Research Center (Beaverton, OR, USA) and the primate facility of the Gene Therapy Program at the University of Pennsylvania (Philadelphia, PA, USA). (cdc.gov)
For example, the rhesus isolate AAV8 has shown improved gene transfer to liver, muscle, and photoreceptors (1, 4) whereas the human isolate AAV9 is capable of crossing endothelial and blood-brain barriers that enable it to target the heart and central nervous system (5, 6). (upenn.edu)

We recently engineered a murine model for this progressive disease and we investigated the possibility of preventing the development of muscular dystrophy in these animals by adenovirus-mediated gene transfer of human alpha-sarcoglycan. (ncl.ac.uk)
The combined gene transfer increased splenic NK cell activity and IFN-γ production by splenocytes. (biomedcentral.com)
The results suggest that adenovirus-mediated IL-12 gene transfer combined with Ad.CD followed by 5-FC treatment may be useful for treating cancers. (biomedcentral.com)

Anatomy12

Organisms24

Diseases13

Chemicals and Drugs33

Analytical, Diagnostic and Therapeutic Techniques and Equipment17

Phenomena and Processes30

Information Science3

Adeno-assoc3

Recombinant adenovirus3

Vectors17

Protein21

Infection16

Serotype1

Vector10

Genome7

Hexon and fiber genes1

Epithelial cells1

Novel Adenoviruses1

Infect1

Simian10

Polymerase chain re1

Therapeutic6

Proteins4

Vivo gene1

Asymptomatic3

Species5

Viruses6

Adenoviral2

Beta-galactosidase1

Regulation4

20231

Isolated human adenoviru1

Strains1

Respiratory3

Differentially expressed1

Skeletal muscle1

Molecular2

Characterization1

Innate2

Replication3

Cells13

Humans1

Interferon1

Genetic2

Fecal1

Pathogens2

Clinically1

Neuraminidase2

Biomarkers1

Mice3

Specimens3

Therapy for cancer treatment1

20163

Monogenic1

Human Gene Th1

Serotypes2

Homologous1

Systemic1

Clinical3

Sequence1

Mutations5

Rhesus3

Transfer3