Adenovirus expression. Medical search. Frequent questions

Infection of a panel of human melanoma cell lines by AdCMVMAGE-1, a novel recombinant adenovirus which incorporates the full-length MAGE-1 cDNA, was shown to induce production of high levels of MAGE-1 protein. (illinois.edu)
BACKGROUND: Immunization of BALB/c mice with a recombinant adenovirus expressing Mycobacterium tuberculosis (M. tuberculosis) antigen 85A (Ad85A) protects against aerosol challenge with M. tuberculosis only when it is administered intra-nasally (i.n. (ox.ac.uk)
The recombinant adenovirus bearing S-protein gene was generated by ligation method. (elsevierpure.com)
Recombinant adenovirus type 5 (rAd) has been used as a vaccine platform against many infectious diseases and has been shown to be an effective vaccine vector. (mdpi.com)
We have constructed a recombinant adenovirus, Ad-hUGT1A1-CTLA4Ig that coexpresses human bilirubin-uridinediphosphoglucuronate glucuronosyltransferase (hUGT1A1) and soluble murine CTLA4Ig, both driven by CMV immediate-early promoters. (nih.gov)
Recombinant adenovirus also called Ad52bclxs/loxp. (umich.edu)

The best PSP was selected to construct a prostate-specific oncolytic adenovirus (CRAD) by controlling the adenoviral E1 region. (hindawi.com)
The oncolytic adenovirus ICOVIR-15K was engineered to express an EGFR-targeting BiTE (cBiTE) antibody under the control of the major late promoter, leading to generation of ICOVIR-15K-cBiTE, which retained its oncolytic properties in vitro . (aacrjournals.org)

CONCLUSIONS: Our microarray analysis represents the first ex vivo study comparing gene expression profiles of CD8 T cells isolated from distinct sites after immunization with an adenoviral vector by different routes. (ox.ac.uk)
Aim: To develop a highly efficacious method for preparation of soluble SARS S-protein using adenovirus vector to meet the requirement for S-protein investigation. (elsevierpure.com)
Methods: The human adenovirus vector was used to express the soluble S-protein (corresponding to 1-1190 amino acids) fused with Myc/His tag using codon-optimized gene construct in HEK239 cells. (elsevierpure.com)
The objective of the work presented in Chicago was to evaluate CVMR-BAC as an expression vector of foreign genes. (thepoultrysite.com)
By using an expression vector, a 680 bp PSA promoter isolated from a prostate cancer patient demonstrated the prostate-specific expression of the reporter gene [ 9 ]. (hindawi.com)
Host immune responses limit the duration of expression of transgenes introduced by recombinant adenoviruses, preclude gene transfer upon vector readministration and cause liver injury. (nih.gov)
This is the first demonstration that coexpression of CTLA4Ig permits prolonged expression and repeatable gene transfer by an adenoviral vector. (nih.gov)
Using bcl-2-expressing adenovirus vector we investigated the effect of Bcl-2 on UVB-induced apoptosis. (bioone.org)
In this manuscript, we have developed a recombinant human adenovirus as an expression vector that contains the gene for porcine interferon alpha. (usda.gov)
An E1/E3 deleted adenovirus type 5 vector expressing cre recombinase under CMV promoter control. (umich.edu)

To evaluate the expression of the coxsackie and adenovirus receptor (CAR) and α v integrins in clinical specimens of bladder cancer to determine the susceptibility to adenoviral gene therapy. (elsevierpure.com)
The protein encoded by this gene is a type I membrane receptor for group B coxsackie viruses and subgroup C adenoviruses. (wikipedia.org)
Many clinically relevant tissues are refractory to Ad5 transduction because of negligible levels of the primary Ad5 receptor, the coxsackie and adenovirus receptor (CAR). (cnrs.fr)

CAR is structurally a member of the Ig-type superfamily of cell-cell adhesion molecules, suggesting that its expression may also be related to the state of tumor differentiation. (elsevierpure.com)
We performed immunohistochemistry for CAR and integrin α v expression in bladder cancer specimens in 50 paraffin-embedded tumor-normal pairs and confirmed the results by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of 11 separate bladder tumors and 4 separate normal bladder controls. (elsevierpure.com)
Moreover, CAR expression is not found in normal or tumor cell lines. (wikipedia.org)
The present study revealed that E2F1 gene expression correlates with TS and Survivin gene expressions and tumor proliferation. (aacrjournals.org)
Despite the existence of a highly immunosuppressive tumor environment, adenovirus-infected cells can nonetheless be efficiently cleared by infiltrating cytotoxic T lymphocytes (CTL) without compromising tumor burden. (aacrjournals.org)
In this study, we tested the hypothesis that tumor-infiltrating T cells could be more effectively activated and redirected by oncolytic adenoviruses that were armed with bispecific T-cell-engager (BiTE) antibodies. (aacrjournals.org)
Notably, intratumoral injection of this cBiTE-expressing adenovirus increased the persistence and accumulation of tumor-infiltrating T cells in vivo , compared with the parental virus lacking such effects. (aacrjournals.org)
Role of Fas ligand expression in promoting escape from immune rejection in a spontaneous tumor model. (southernbiotech.com)

Also, viral infection has proved to have a great impact on cellular small RNA expression and gene expression [ 9 - 11 ]. (hindawi.com)
Small RNAs include microRNA (miRNA), siRNA, tRFs, piRNA, and rasiRNAs, which regulate gene expression in a wide range of processes such as viral replication and host immune response. (hindawi.com)
miRNAs regulate gene expression in a wide range of physiological and pathological processes such as in immune response and viral replication [ 18 ]. (hindawi.com)
We conclude that the association and altered regulation of IRS proteins by Ad5E1A contribute to the adenovirus-transformed phenotype and modulates viral infection in an Akt-dependent manner. (ox.ac.uk)
The molecular steps in the replication pathway that regulate the levels of viral gene expression are not well defined. (microbiologyresearch.org)
We have investigated potential mechanisms of regulation of rotavirus gene expression by functional evaluation of two differentially expressed viral mRNAs. (microbiologyresearch.org)
These data define a regulatory mechanism for the difference in amounts of VP6 and NSP1 and provide evidence for post-transcriptional control of rotavirus gene expression mediated by the translational efficiency of individual viral mRNAs. (microbiologyresearch.org)
To overcome this obstacle, conditional oncolytic viruses (such as conditional replication adenovirus (CRAD)) are developed to specifically target prostate without (or with minimal) systemic toxicity due to viral self-replication. (hindawi.com)
The adenovirus early region 1 (E1) gene, which comprises E1a and E1b, encodes the viral early proteins that are necessary for adenoviral replication and the consequent oncolysis of permissive host cells. (hindawi.com)
E1-deleted (including E1a-deleted) adenoviruses are replication defective and are commonly used as viral vectors to carry therapeutic genes for gene therapy. (hindawi.com)
When amplifying purified adenovirus that has been serially propagated it may be wise to isolate viral clones (with a plaque amplification), in order to avoid any possible problems with replication-competent adenovirus. (umich.edu)
Adenovirus DNA : the viral genome and its expression / edited by Walter Doerfler. (who.int)
Proteínas transcritas a partir de la región E1B del genoma de ADENOVIRUS, que estan involucradas en la regulación de los niveles precoces y tardios de expresión genética viral. (bvsalud.org)
Proteins transcribed from the E1B region of ADENOVIRUSES which are involved in regulation of the levels of early and late viral gene expression. (bvsalud.org)

In response to a brain injury, astrocytes proliferate and become hypertrophic with an increased expression of intermediate filament proteins. (jneurosci.org)

Pre-made h OCT4 expression Adenovirus, expressing human stem cell factor, SOX2 gene under suCMV promoter . (gentarget.com)

Studies from human hearts have shown that lower expression of CXADR mRNA is associated with a risk allele at chromosome 21q21, which may in fact predispose hearts to arrhythmias. (wikipedia.org)
Prolonged expression of Ad5E1A13S is required for the observed dramatic increase in the levels of IRS-4 mRNA and protein in Ad5E1-transformed human cell lines. (ox.ac.uk)
Methods and results: In primary cultured rat stellate cells and the LX-2 human stellate cell line, induction of BMP-7 by Ad-BMP-7 infection decreased the expression of collagen 1A2 mRNA and smooth muscle α-actin in the presence or absence of TGFβ, via Smad 1/5/8 phosphorylation. (mssm.edu)
BMP-7 triggered the mRNA expression of inhibitors of differentiation 2 (Id2) in LX-2. (mssm.edu)
Slovania Nurse tell what the codes on the vaccine vials are Saline Adenovirus & MRNA. (abovetopsecret.com)

The recombinant HVT-VP2 viruses were stable and showed continuous expression of VP2 even after several passages in cell culture. (thepoultrysite.com)
For successful transgene expression, viruses administered into muscle must undergo a series of processes, including host cell interaction and internalization, intracellular sorting, long-range retrograde axonal transport, endosomal liberation, and nuclear import. (frontiersin.org)
In this review article, we outline key characteristics of major gene therapy viruses-adenovirus, adeno-associated virus (AAV), and lentivirus-and summarize the mechanisms regulating important steps in the virus journey from binding at peripheral nerve terminals to nuclear delivery. (frontiersin.org)
Subsequently, with the discovery of Norwalk virus ( 1 ), rotavirus ( 2 ), astrovirus ( 3,4 ), and enteric adenovirus ( 5 ), researchers began to recognize viruses as causative agents of gastroenteritis. (cdc.gov)

View cart "h SOX2 expression Adenovirus, in vivo ready" has been added to your cart. (gentarget.com)

Increased OLR1 expression, resulting either from TZD treatment or adenoviral gene delivery, significantly augments adipocyte cholesterol content and enhances fatty acid uptake. (jci.org)

Photoreceptor-specific transgene expression was accomplished through incorporation of the 2.5 kb human rhodopsin upstream fragment (HRP). (nih.gov)

Adv infects the host cells through binding to different cellular receptors such as coxsackievirus and adenovirus receptor (CAR) [ 12 ]. (hindawi.com)
Coxsackievirus and adenovirus receptor (CAR) is a protein that in humans is encoded by the CXADR gene. (wikipedia.org)

We tested the possibility of interfering with apoptotic photoreceptor cell death in the rd mouse through subretinal delivery of a recombinant replication-defective adenovirus containing the human cDNA for bcl-2, Ad.2.5HRPbcl-2. (nih.gov)
The idea behind this study is to place the Ad5 E1 region in cis complementation (i.e., use E1 as a transgene) back into an E1-deleted, replication-defective adenovirus under the control of a prostate-specific promoter (PSP). (hindawi.com)

Up to 12, 1 ml amplified clones of adenovirus (primary lysate). (umich.edu)

Human adenovirus (Adv) infection is responsible for most community-acquired pneumonia in infants and children, which results in significant morbidity and mortality in children every year. (hindawi.com)
Knowing the miRNA expression profile will help understand the role of miRNAs in modulating the host response to adenovirus infection and possibly improve the diagnosis of adenovirus-infected pneumonia. (hindawi.com)
Human adenovirus (Adv) infection is responsible for most community-acquired pneumonia in infants and children [ 1 , 2 ]. (hindawi.com)
The present report concerns the role of adenovirus infection in inducing this transformation in an animal model of chronic obstructive pulmonary disease. (ersjournals.com)
These human observations are supported by experiments in guinea pigs, where, in the absence of actively replicating virus but the presence of E1A DNA, adenovirus infection resulted in excess lung inflammation following a single exposure to cigarette smoke 7 , and increased parenchymal and airway wall inflammation with greater emphysematous destruction after chronic exposure 8 . (ersjournals.com)
In the present study, the hypothesis that adenoviral infection followed by persistent expression of its E1A gene is capable of producing epithelial-mesenchymal transformation was examined by investigating guinea pig PLECs that express adenoviral E1A. (ersjournals.com)
Results: Under the conditions of infection dose (MOI of 50) and expression time (48 h), the high-level expression of S-protein was obtained. (elsevierpure.com)

Expression of TAP1 is down-regulated by human Epstein-Barr virus vil-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules. (lu.se)

High levels of adenovirus-specific antibodies and CTL, and hepatic inflammation were found. (nih.gov)

It is postulated that this intermediate phenotype may play a major role in the remodelling of the airways in chronic obstructive pulmonary disease associated with persistence of adenovirus E1A DNA. (ersjournals.com)

In order to determine why the i.n. route is so effective compared to parenteral immunization, we used microarray analysis to compare gene expression profiles of pulmonary and splenic CD8 T cells after i.n. or intra-dermal (i.d.) immunization. (ox.ac.uk)
The sustained expression of chemokines and activation genes enables CD8 T cells to remain in the lungs for extended periods after i.n. immunization. (ox.ac.uk)

The rat PB gene promoter (454 bp) contains ARE [ 11 ] and directs a hormonal and developmental regulated expression, of a heterologous gene, specifically to the prostate in transgenic mice [ 12 ]. (hindawi.com)

We examined hepatocellular carcinoma (HCC) development in alcohol Western diet-fed hepatitis C virus NS5A Tg mice with hepatocyte-specific TBC1D15 deficiency or expression of non-phosphorylatable NUMB mutations. (nature.com)
Physical mapping of temperature-sensitive mutations of adenoviruses. (cshlpress.com)
Scope includes mutations and abnormal protein expression. (cancerindex.org)

This study also demonstrated that the Hedgehog agonist is active in human scalp in vitro as measured by Hedgehog pathway gene expression. (bernsteinmedical.com)
Postinternalization inhibition of adenovirus gene expression and infectious virus production in human T-cell lines. (southernbiotech.com)
Inhibited by human adenovirus e3-19k glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. (lu.se)

Loss of CAR expression correlates with the invasive phenotype in our analysis of bladder cancer. (elsevierpure.com)

These highly upregulated microRNAs may have crucial roles in Adv pathogenesis and are potential biomarkers for adenovirus-infected pneumonia. (hindawi.com)

In this study, recombinant (r) adenoviruses (Ads) encoding full-length and truncated rHu BChEs were tested for in vivo expression in mice. (nebraska.edu)
Chemokine gene expression in lung CD8 T cells correlates with protective immunity in mice immunized intra-nasally with Adenovirus-85A. (ox.ac.uk)
Effect of Adenovirus-Mediated Expression of Sonic Hedgehog Gene on Hair Regrowth in Mice With Chemotherapy-Induced Alopecia. (bernsteinmedical.com)

The G-CSF was delivered by inserting the G-CSF gene into an adenovirus that is safe in humans and known to infect brain cells efficiently. (neurosciencenews.com)

The objective of work presented by Sanjay M. Reddy of Texas A&M University was to evaluate potential cloning sites in the HVT genome for stable expression of foreign genes. (thepoultrysite.com)
In this study, we assayed the effect of exogenous mouse recombinant GDF-5 on cell proliferation of dental pulp cells and determined the expression levels of odontoblast differentiation marker genes in the cells of isolated mouse dental pulp tissues in the presence of GDF-5. (ispub.com)
Here, we investigated the effect of GDF-5 on the proliferation of dental pulp cells and their differentiation into odontoblasts by assaying the expression levels of odontoblast marker genes. (ispub.com)
We investigated the clinical significance of E2F1 gene expression in relation to its target genes, thymidylate synthase ( TS ) and Survivin , in case of non-small-cell lung cancer (NSCLC). (aacrjournals.org)
Intriguingly, impairment of eIF2α phosphorylation produced decreases in the starvation-induced expression of UPR and autophagy genes in AgRP neurons. (bvsalud.org)

Case-control studies have shown that the E1A DNA from group C adenoviruses is present in greater amounts in the lungs of patients with COPD than in controls with normal lung function matched for age, sex and smoking history 5 , and that the excess inflammation in the lungs of smokers with severe emphysema is associated with increased numbers of alveolar epithelial cells expressing E1A 6 . (ersjournals.com)

Thus, E1 protein expression will be confined strictly to the prostate tissues and render this a conditional oncolytic virus (CRAD) within the prostate. (hindawi.com)
Along with other BMPs, including BMP-2, -4 and -7, which are well known as potent osteoinductive growth factors, GDF-5 plays important roles in the development of bones, cartilage and tendons, as evidenced by the tendency for the gene and protein expression levels of GDF-5 to increase over time in these tissues. (ispub.com)
Immunohistochemistry was done to investigate the protein expression of E2F1, TS, and Survivin. (aacrjournals.org)

Guinea pig primary peripheral lung epithelial cells (PLECs) transfected with adenovirus E1A (E1A-PLECs) were compared to guinea pig normal lung fibroblasts (NLFs) transfected with E1A (E1A-NLFs). (ersjournals.com)
Simultaneously, the finding of increased α v expression in invasive cancer suggests a pathogenesis that involves heterophilic adhesion and migration of these cells on various extracellular ligands. (elsevierpure.com)
Expression of CAR in endothelial cells can be regulated by treatment with drugs. (wikipedia.org)
Expression of CXCR6 on CD8 T cells was confirmed by flow cytometry. (ox.ac.uk)
The expression level was determined to be approximately 75 μg/10 6 cells after purification. (elsevierpure.com)
Conclusion: The high-level expression of S-protein in HEK293 cells mediated by adenovirus can be achieved under the optimized expression conditions. (elsevierpure.com)
In zebrafishes, forced expression of rat lcn2 gene increased the number and thickness of cellular processes in GFAP-expressing radial glia cells, suggesting that lcn2 expression in glia cells plays an important role in vivo . (jneurosci.org)
Bcl-2-transfected NMK cells were significantly resistant to UVB-induced apoptosis with the suppressive effect dependent on the Bcl-2 expression level. (bioone.org)
cBiTE expression and secretion was detected in supernatants from ICOVIR-15K-cBiTE-infected cells, and the secreted BiTEs bound specifically to both CD3 + and EGFR + cells. (aacrjournals.org)

Aim: In this study, we examined whether adenovirus-mediated overexpression of BMP-7 (Ad-BMP-7) antagonised the effect of TGFβ in vitro and in vivo. (mssm.edu)

lcn2 expression and secretion increased after inflammatory stimulation in cultured astrocytes. (jneurosci.org)

Nucleotide sequence and expression in Escherichia coli of the gene encoding the nonstructural protein NCVP2 of bovine rotavirus. (microbiologyresearch.org)

The Toronto strain of canine adenovirus type 2 (CAV2) exhibits native tropism that is augmented by, but not fully dependent upon, CAR for cellular transduction. (cnrs.fr)

Together, our data emphasize the utility of adenoviruses as vaccination vehicles and highlight the potential efficacy of this approach for the treatment of melanoma. (illinois.edu)

In our study, total RNA extracted from whole blood of adenovirus-infected pneumonia children and healthy controls were analyzed by small RNA deep sequencing. (hindawi.com)

Quantitative reverse transcription-PCR was done to evaluate the gene expression of E2F1, TS , and Survivin . (aacrjournals.org)

Search the gene expression profiles from curated DataSets in the Gene Expression Omnibus (GEO) repository. (cancerindex.org)

In this Perspectives article, we review and synthesize these recent lines of evidence and propose a working nomenclature for SCLC subtypes defined by relative expression of these four factors. (cancerindex.org)

Studies on bladder cancer cell lines have shown that low adenoviral transduction rates were associated with low-level expression of CAR. (elsevierpure.com)
Antiviral immune responses present a major hurdle to the efficacious use of oncolytic adenoviruses as cancer treatments. (aacrjournals.org)

The top 3 upregulated miRNA (hsa-miR-127-3p, hsa-miR-493-5p, and hsa-miR-409-3p) were identified in adenovirus-infected children and provided a clear distinction between infected and healthy individuals. (hindawi.com)

In this process, astrocytes proliferate to fill gaps and undergo a typical morphological change: a large cytoplasmic mass, long and branched processes, and increased expression of intermediate filaments such as glial fibrillary acidic protein (GFAP). (jneurosci.org)

Gene expression profiling in pulmonary hypertension. (famri.org)

In contrast, control animals inoculated with an adenovirus lacking the interferon gene developed typical signs of FMD. (usda.gov)

In addition, we propose that loss of CAR expression decreases rigid cell adhesion, possibly increasing the migratory potential. (elsevierpure.com)
Reducing IRS-4 expression using small interfering RNA (siRNA) in established Ad5E1A-expressing cell lines decreases the activation of Akt and cellular proliferation. (ox.ac.uk)

Integrin α v expression increases in various tumors suggest its importance in differentiation, proliferation, and migration. (elsevierpure.com)

Anatomy2

Organisms8

Diseases4

Chemicals and Drugs12

Analytical, Diagnostic and Therapeutic Techniques and Equipment4

Phenomena and Processes5

Information Science2

Recombinant adenovirus6

Oncolytic adenovirus2

Vector10

Coxsackie3

Tumor8

Viral14

Proteins1

OCT41

MRNA5

Viruses4

Vivo ready1

Adenoviral gene1

Transgene expression1

Coxsackievirus2

Replication2

Clones1

Infection7

Peptide1

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Persistence1

Immunization2

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Mutations3

Human3

Phenotype1

Biomarkers1

Mice3

Efficiently1

Genes5

Inflammation1

Protein expression3

Cells9

Vitro1

Secretion1

Rotavirus1

Type1

Efficacy1

Study1

Evaluate1

Gene expression profiles1

Factors1

Cancer2

MiRNA1

Undergo1

Pulmonary1

Control1

Decreases2

Differentiation1