• Post-Transcriptional detargeting Another layer of regulation that has emerged to control adenoviral replication is the use of microRNAs (miRNA) artificial target sites or miRNA response elements (MREs). (wikipedia.org)
  • E1B-55K is a phosphorylation-dependent transcriptional and post-transcriptional regulator of viral gene expression in HAdV-C5 infection. (leibniz-liv.de)
  • For adenovirus replication to occur, the host cell must be induced into S phase by viral proteins interfering with cell cycle proteins. (wikipedia.org)
  • AdCox2Lluc is a CRAd targeted against oesophageal adenocarcinoma by placing the early genes under the control of a Cox-2 promoter (adenoviruses have two early genes, E1A and E1B, that are essential for replication). (wikipedia.org)
  • Adipocyte commitment of 3T3-L1 cells is required to support human adenovirus 36 productive replication concurrent with altered lipid and glucose metabolism. (leibniz-liv.de)
  • Evidence that the Adenovirus Single-Stranded DNA Binding Protein Mediates the Assembly of Biomolecular Condensates to Form Viral Replication Compartments. (leibniz-liv.de)
  • One-step growth curve showed that the replication of the SAdVs with HAdV-5 E4orf6 substitution and E1/E3 deletion was similar to that of wild-type SAdVs in HEK293 cells, but the modified SAdVs were replication-deficient in A549 cells which lack HAdV-5 E1A and E1B. (bvsalud.org)
  • Of the many different viruses being explored for oncolytic potential, an adenovirus was the first to be approved by a regulatory agency, the genetically modified H101 strain. (wikipedia.org)
  • Approximately 70 years ago, several DNA viruses such as adenovirus, human Epstein-Barr virus, polyoma and SV40 were found to be able to cause tumors in humans and rodents [ 1 ] . (encyclopedia.pub)
  • Adenovirus varieties have been explored extensively as a viral vector for gene therapy and also as an oncolytic virus. (wikipedia.org)
  • The adenovirus E1B55kDa gene cooperates with another adenoviral product, E4ORF6, to inactivate p53, thus preventing apoptosis. (wikipedia.org)
  • It was initially proposed that an adenovirus mutant lacking the E1B55kDa gene, dl1520 (ONYX-015), could replicate selectively in p53 deficient cells. (wikipedia.org)
  • Both human and non-human simian adenoviruses (HAdVs and SAdVs, respectively) have been used as gene therapy and vaccine vectors. (bvsalud.org)
  • citation needed] A conditionally replicative adenovirus (CRAd) with a 24 base pair deletion in the retinoblastoma-binding domain of the E1A protein (Ad5- Δ24E3), is unable to silence retinoblastoma, and therefore unable to induce S-phase in host cells. (wikipedia.org)
  • citation needed] The most commonly used group of adenoviruses is serotype 5 (Ad5), whose binding to host cells is initiated by interactions between the cellular coxsackie virus and adenovirus receptor (CAR), and the knob domain of the adenovirus coat protein trimer. (wikipedia.org)
  • These molecules are fusion proteins that are made up of an antibody raised against the knob domain of the adenovirus coat protein, fused to a natural ligand for a cell-surface receptor. (wikipedia.org)
  • The fiber protein also contains an HI-loop structure, which can tolerate peptide insertions of up to 100 residues without any negative effects on adenovirus integrity. (wikipedia.org)
  • Protein-Protein Interactions Facilitate E4orf6-Dependent Regulation of E1B-55K SUMOylation in HAdV-C5 Infection. (leibniz-liv.de)
  • Human Adenovirus Type 5 Infection Leads to Nuclear Envelope Destabilization and Membrane Permeability Independently of Adenovirus Death Protein. (leibniz-liv.de)
  • Conserved E1B-55K SUMOylation in different human adenovirus species is a potent regulator of intracellular localization. (leibniz-liv.de)
  • Animal Models in Human Adenovirus Research. (leibniz-liv.de)
  • KIR3DS1 directs NK cell-mediated protection against human adenovirus infections. (leibniz-liv.de)
  • citation needed] Transcriptional targeting This approach takes advantage of deregulated promoter to drive and control the expression of adenoviral genes. (wikipedia.org)
  • Adenovirus early region E1B encodes two proteins that act to prevent p53-mediated apoptosis, and hence promote viral replication. (medscape.com)
  • 9. Adenovirus-mediated overexpression of the transcription factor E2F-1 induces apoptosis in human breast and ovarian carcinoma cell lines and does not require p53. (nih.gov)
  • 10. Induction of transformation and p53-dependent apoptosis by adenovirus type 5 E4orf6/7 cDNA. (nih.gov)
  • The adenovirus E1B55kDa gene cooperates with another adenoviral product, E4ORF6, to inactivate p53, thus preventing apoptosis. (wikipedia.org)
  • Oncoproteins encoded by the early region of adenoviruses have been shown to be powerful tools to study gene regulatory mechanisms, which affect major cellular events such as proliferation, differentiation, apoptosis and oncogenic transformation. (biomedcentral.com)
  • H. (2021) Silencing of Mcl-1 Overcomes Resistance of Melanoma Cells Against TRAIL-armed Oncolytic Adenovirus by Enhancement of Apoptosis. (tu.berlin)
  • The ability of adenovirus early region proteins, E1B-55K and E4orf6, to usurp control of cellular ubiquitin ligases and target proteins for proteasome-dependent degradation during infection is well established. (birmingham.ac.uk)
  • The adenovirus genome is contained within a capsid comprising 12 penton and 240 hexon subunits, plus minor structural proteins. (medscape.com)
  • For adenovirus replication to occur, the host cell must be induced into S phase by viral proteins interfering with cell cycle proteins. (wikipedia.org)
  • These molecules are fusion proteins that are made up of an antibody raised against the knob domain of the adenovirus coat protein, fused to a natural ligand for a cell-surface receptor. (wikipedia.org)
  • The adenovirus type 5 (HAdV-C5) E1 transcription unit encodes regulatory proteins that are essential for viral replication and transformation. (nih.gov)
  • Recently we and others have shown that two additional proteins of early region 4 (Ad5 E4orf6 and E4orf3) possess transforming and oncogenic potential. (uni-regensburg.de)
  • Proteins encoded by the early transcription unit 1A (E1A) of Adenovirus (Ad) are essential for the viral life cycle because of their necessity in regulating the expression of all other viral genes [ 1 ]. (biomedcentral.com)
  • Indeed, TIF1 gamma knockdown accelerates the adenovirus-mediated degradation of MRE11, while TIF1 gamma overexpression delays the adenovirus-mediated degradation of MRE11. (birmingham.ac.uk)
  • Human adenoviruses have been divided into 49 serotypes and these have been organized into six subgroups according to their ability to agglutinate red blood cells( Table 1 ). (medscape.com)
  • CR3 is one of four E1A regions (CR1, CR2, CR3 and CR4) that are highly conserved among all Adenovirus serotypes [ 2 ]. (biomedcentral.com)
  • citation needed] Transcriptional targeting This approach takes advantage of deregulated promoter to drive and control the expression of adenoviral genes. (wikipedia.org)
  • Post-Transcriptional detargeting Another layer of regulation that has emerged to control adenoviral replication is the use of microRNAs (miRNA) artificial target sites or miRNA response elements (MREs). (wikipedia.org)
  • Moreover, these findings suggest that FAM111B might play an important role in the host antiviral immune response that is counteracted by HAdV-C5 E1B-55K and E4orf6 oncoproteins. (nih.gov)
  • The use of a number of different adenovirus early region mutants identified the specific and sole requirement for E4orf3 in mediating TIF1 gamma degradation. (birmingham.ac.uk)
  • Further analyses revealed that TIF1 gamma is targeted for degradation by a number of divergent human adenoviruses, suggesting that the ability of E4orf3 to regulate TIF1 gamma expression is evolutionarily conserved. (birmingham.ac.uk)
  • Die in der vorliegenden Arbeit dargestellten Untersuchungen zum transformierenden Potenzial humaner Adenoviren zeigen erstmals, dass zwei virale Genprodukte der frühen Region 4 - E4orf3 und E4orf6 - von Adenovirus Serotyp 5 transformierende Eigenschaften aufweisen, die zur 'Hit & Run'-Transformation primärer BRK-Zellen beitragen. (uni-regensburg.de)
  • METHODS: Epigenetic, transcriptional and metabolic targets were analysed in different cellular and animal models with stable knockdown or knockout of PK. (bvsalud.org)
  • Oncolytic viral therapy has mainly used group C adenoviruses, primarily Ad2 and Ad5. (medscape.com)
  • Adenovirus varieties have been explored extensively as a viral vector for gene therapy and also as an oncolytic virus. (wikipedia.org)
  • Of the many different viruses being explored for oncolytic potential, an adenovirus was the first to be approved by a regulatory agency, the genetically modified H101 strain. (wikipedia.org)
  • [ 16 ] The resultant stabilization of p53 arrests the cell in G1 via transcriptional activation of the cdk inhibitor p21 Cip1 , [ 17 ] or causes cell death via the intrinsic apoptotic pathway by transactivating cellular proapoptotic genes Bax and Bak . (medscape.com)
  • citation needed] The most commonly used group of adenoviruses is serotype 5 (Ad5), whose binding to host cells is initiated by interactions between the cellular coxsackie virus and adenovirus receptor (CAR), and the knob domain of the adenovirus coat protein trimer. (wikipedia.org)
  • The transforming potential of human adenoviruses has been traditionally ascribed to the E1A and E1B transcription units encoded within early region 1 (E1). (uni-regensburg.de)
  • However, there is increasing interest in the use of group B adenoviruses, which will be discussed later. (medscape.com)
  • The HBV core protein mediates rcDNA transport to the nucleus and likely affects stability and transcriptional activity of cccDNA. (bvsalud.org)