Schematic of the adenovirus genome and adenovirus-based vectors. (genemedi.com)
Adenovirus (Ad) vectors are among the most commonly used viral vectors in gene therapy clinical trials because they have the following advantages. (hindawi.com)
On the other hand, Ad vectors also have a number of disadvantages, including their low transduction efficiency in cells lacking coxsackievirus-adenovirus receptor (CAR) and their induction of innate immune responses. (hindawi.com)
In order to overcome these disadvantages of the first-generation Ad vectors, next-generation Ad vectors, including fiber-mutant Ad vectors and helper-dependent Ad vectors, have been developed [ 6 - 8 ]. (hindawi.com)
c production of replication incompetent AAV vectors via transient plasmid transfection into 293 producer cells that express adenovirus type 5 E1 genes. (biomedcentral.com)
Genome3
It also exhaustively introduces the concrete mechanism of invading GC cells and the viral genome composition of adenovirus and herpes simplex virus type 1 (HSV-1). (frontiersin.org)
Top panel: a simplified map of the adenovirus serotype 5 genome showing the early genes (E1-E4) and the region from which the major late transcript is produced. (genemedi.com)
Without integration into host genome, adenovirus genome remains in an episomal state, which guarantees the low risk of mutation (Fig. 8). (genemedi.com)
Recombinant1
To prevent infected cell lysis, recombinant replication deficiency virus has been developed as a gene delivery tool to replace wild-type adenovirus (recombinant AdV in "application" part). (genemedi.com)
Viral4
To investigate this issue, we examined properties of p53 in normal human cells infected by E1B mutant viruses that display defective entry into the late phase or viral late mRNA export. (princeton.edu)
Adenovirus transcription is a two-phase event, early and late, occurring before and after viral DNA replication, respectively (Figure. (genemedi.com)
For most serotypes, adenovirus infection is mediated by the high-affinity binding of the fiber-knob region to a receptor of target cell, named as the coxsackie-Ad receptor (CAR), which mainly determines the viral tropism [44]. (genemedi.com)
Life cycle of adenovirus is separated by DNA replication process into two distinct phases: the early and late, occurring before and after viral DNA replication, respectively. (genemedi.com)
Defective1
Pseudorabies Virus Mutants Lacking US9 Are Defective in Cytoplasmic Assembly and Sorting of Virus Particles into Axons and Not in Axonal Transport. (viictr.org)
Mice1
In vivo studies of HDL assembly and metabolism using adenovirus-mediated transfer of ApoA-I mutants in ApoA-I-deficient mice. (uchicago.edu)
Mutation1
NA-inhibition assays showed that all the single and double mutants containing the Y275 except the single Y275-E119V mutant conferred important levels of resistance to oseltamivir, whereas all the single, double, and triple mutants containing the E119V mutation were associated with the resistance to zanamivir. (ophrp.org)
Protein4
It is well established that the human subgroup C adenovirus type 5 (Ad5) E1B 55 kDa protein can regulate the activity and concentration of the cellular tumor suppressor, p53. (princeton.edu)
The steady-state concentrations of p53 were significantly higher in cells infected by the E1B 55 kDa null mutant Hr6 or three mutants carrying small insertions in the E1B 55 kDa protein coding sequence than in Ad5-infected cells. (princeton.edu)
Rather, the localization of p53 to E1B containing nuclear sites observed during infection by Ad5 was prevented by mutations that impair interaction of the E1B protein with p53 and/or with the E4 Orf6 protein. (princeton.edu)
We have previously shown that the adenovirus (Ad) E4orf4 protein inhibits DDR signaling, but the mechanisms were not identified. (grandlacs-med-journal.com)
Type1
Wild-type p110α has more interactions with the nSH2 domain than the oncogenic mutant H1047R. (oncotarget.com)
A) HeLa cells were either mock infected or infected with the Ad mutant lacking the whole E4 region or expressing E4orf4 as the only E4 ORF. (grandlacs-med-journal.com)
Human2
Lipoproteins produced by ApoE-/- astrocytes infected with adenovirus expressing human ApoE. (uchicago.edu)
Adenovirus (AdV) is a member of the family Adenoviridae, whose name derives from their initial isolation from human adenoids in 1953 [42]. (genemedi.com)
Apoptosis induci1
E1B and E4 oncoproteins of adenovirus antagonize the effect of apoptosis inducing factor. (wakehealth.edu)
E4orf63
The ability of adenovirus early region proteins, E1B-55K and E4orf6, to usurp control of cellular ubiquitin ligases and target proteins for proteasome-dependent degradation during infection is well established. (birmingham.ac.uk)
Here we show that the E4 gene product, E4orf3 can, independently of E1B-55K and E4orf6, target the transcriptional corepressor transcriptional intermediary factor 1 gamma (TIF1 gamma) for proteasome-mediated degradation during infection. (birmingham.ac.uk)
Die in der vorliegenden Arbeit dargestellten Untersuchungen zum transformierenden Potenzial humaner Adenoviren zeigen erstmals, dass zwei virale Genprodukte der frühen Region 4 - E4orf3 und E4orf6 - von Adenovirus Serotyp 5 transformierende Eigenschaften aufweisen, die zur 'Hit & Run'-Transformation primärer BRK-Zellen beitragen. (uni-regensburg.de)
2. In vitro and in vivo biology of recombinant adenovirus vectors with E1, E1/E2A, or E1/E4 deleted. (nih.gov)
Adenoviruses (Ad) are excellent gene transfer vectors and are extensively used for high-level transgene expression in vitro and in vivo . (biomedcentral.com)
Plasmid1
17. Increased duration of transgene expression in the lung with plasmid DNA vectors harboring adenovirus E4 open reading frame 3. (nih.gov)
Adenoviral vectors2
11. Generation and characterization of E1/E2a/E3/E4-deficient adenoviral vectors encoding human factor VIII. (nih.gov)
20. A simple method for constructing E1- and E1/E4-deleted recombinant adenoviral vectors. (nih.gov)
HELPER VIRUS1
As its name implies, AAV is dependent upon the presence of a helper virus, such as an adenovirus or herpes virus, for active replication. (justia.com)
Oncolytic1
14. Efficient generation of double heterologous promoter controlled oncolytic adenovirus vectors by a single homologous recombination step in Escherichia coli. (nih.gov)
E4orf32
The use of a number of different adenovirus early region mutants identified the specific and sole requirement for E4orf3 in mediating TIF1 gamma degradation. (birmingham.ac.uk)
Further analyses revealed that TIF1 gamma is targeted for degradation by a number of divergent human adenoviruses, suggesting that the ability of E4orf3 to regulate TIF1 gamma expression is evolutionarily conserved. (birmingham.ac.uk)
Antiviral2
Further studies suggested that TIF1 gamma possesses antiviral activity and limits adenovirus early and late gene product expression during infection. (birmingham.ac.uk)
Inactivating intracellular antiviral responses during adenovirus infection. (wakehealth.edu)
The present invention further provides nucleic acids comprising nucleotide sequences that encode mutant capsid proteins, and host cells comprising the nucleic acids. (justia.com)
E4orf41
In transformed cells, induction of apoptosis by adenovirus type 2 (Ad2) early region 4 ORF 4 (E4orf4) correlates with accumulation of E4orf4 in the cell membrane-cytoskeleton fraction. (silverchair.com)
Infection1
Neonatal infection with species C adenoviruses confirmed in viable cord blood lymphocytes. (wakehealth.edu)
Viruses3
A similar defect is reported here for E4 orf6-mutant viruses. (nih.gov)
By contrast, this ratio was equivalent among cells infected during S phase with the wild-type or mutant viruses. (nih.gov)
Studies of small DNA viruses found in various adenovirus preparations: physical, biological, and immunological characteristics. (genemedi.com)
Region2
We report that transcription of the adenovirus 5 E4 region is regulated during the release of paused Pol2 into productive elongation by recruitment of the super-elongation complex, dependent on promoter H3K18/27 acetylation by CBP/p300. (elifesciences.org)
The transforming potential of human adenoviruses has been traditionally ascribed to the E1A and E1B transcription units encoded within early region 1 (E1). (uni-regensburg.de)
Mutations4
The effects of E4 mutations on DNA replication therefore may reflect a role for E4 in the regulation of replication rather than in the process of DNA synthesis. (nih.gov)
The E4 mutant H5d/1014 carries two deletion mutations that together destroy all E4 open reading frames (ORFs) except ORF 4. (nih.gov)
Macmillan Co, however, whether point mutations are as important in the scenario of NSCLC as in BCR-ABL-positive leukemia, as at least in some reported cases, mutant alleles constitute only a small minority of all alleles (151). (dpforex.com)
The present invention further provides libraries of mutant AAV comprising one or more mutations in a capsid gene. (justia.com)
Gene transfer2
8. A gene transfer vector-cell line system for complete functional complementation of adenovirus early regions E1 and E4. (nih.gov)
13. Persistent transgene expression in mouse liver following in vivo gene transfer with a delta E1/delta E4 adenovirus vector. (nih.gov)
Infections1
Analysis of adenovirus infections in synchronized cells. (wakehealth.edu)
Cell4
An E4 orf3-mutant virus was not restricted for growth by the cell cycle. (nih.gov)
4. Efficient dual transcomplementation of adenovirus E1 and E4 regions from a 293-derived cell line expressing a minimal E4 functional unit. (nih.gov)
Inhibition of the Raf/MEK/ERK pathway up-regulates CAR expression in cancer cells, thus improving adenoviral entry and cell killing by adenoviral mutants. (unina.it)
The present invention provides mutant adeno-associated virus (AAV) that exhibit altered capsid properties, e.g., reduced binding to neutralizing antibodies in serum and/or altered heparin binding and/or altered infectivity of particular cell types. (justia.com)
Functional1
dl1520 is a replication competent adenovirus that, due to lack of a functional E1B-55kD gene, can complete its life cycle solely in tumoural cells. (unina.it)
Complex2
Construction of conditionally-replicative Adenovirus (CRAd) is complex and time-consuming. (biomedcentral.com)
Image reconstruction reveals the complex molecular organization of adenovirus. (nih.gov)
Open1
3. Generation of an adenovirus vector lacking E1, e2a, E3, and all of E4 except open reading frame 3. (nih.gov)
Double1
Wernike K, Michelitsch A, Aebischer A, Schaarschmidt U, Konrath A, Nieper H, Sehl J, Teifke JP, Beer M.The Occurrence of a Commercial Npro and Erns Double Mutant BVDV-1 Live-Vaccine Strain in Newborn Calves. (zoonosen.net)
Results1
The catalytically inactive Parkin C431S mutant results in a dead-end intermediate via ubiquitin-oxyester conjugation on Ser431 (Iguchi et al. (healthanddietblog.info)