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  • tumor
  • We have previously reported ( 13 ) that the two most abundantly expressed immediate-early gene products of HCMV, IE1 and IE2 (reviewed in ref. 14 ), inhibit the induction of apoptosis by tumor necrosis factor α (TNF-α) or the adenovirus E1A oncoproteins. (pnas.org)
  • One of the promising strategies for targeting replication of oncolytic adenovirus in tumor cells is to regulate the expression of essential viral genes such as E1a by using tumor- or tissue-specific promoters that are preferentially active in cancer cells. (nih.gov)
  • mutants
  • We determined that although common residues within the N-terminal region can form partial binding sites for these proteins, point mutants were also generated that could discriminate between binding sites. (asm.org)
  • Transcriptional stimulation of the AdE2 and hsp70 promoters by 12SE1A has therefore been thought to involve distinct mechanisms and, in fact, these promoters have been shown to respond differently to some E1A mutants ( 35 ). (asm.org)
  • apoptosis
  • 53 (1), 167-176 (2018) PUBMED 29658564 REMARK GeneRIF: the present study indicated that CtBP2 reduced the susceptibility of ECA109 cells to cisplatin by regulating the expression of apoptosis-related proteins, suggesting that it may be a promising therapeutic target in esophageal squamous cell carcinoma in the future. (genome.jp)
  • nuclear proteins
  • Shown in A) are five nuclear proteins ( LIN-1 , SUR-2 , LIN-25 , EOR-1 , EOR-2 ) that are jointly important for vulval, excretory duct and P12 cell fates. (wormbook.org)
  • Many nuclear proteins possess a nuclear localisation signal (NLS) that is recognised by the importer protein importin-alpha. (eu.org)
  • Therefore, almost all well studied nuclear proteins are transported into the nucleus using active translocation through the pore. (eu.org)
  • vivo
  • This model of regulation by ZEB proteins also functions in vivo , where they have opposing effects on the regulation of TGFβ family‐dependent genes during Xenopus development. (embopress.org)
  • correlates
  • The ability of E1A to reprogram cellular gene expression to promote entry into S phase correlates with the ability of E1A to cooperate with oncogenes, such as ras , to transform primary cells ( 38 , 62 ). (asm.org)
  • cooperate
  • AdE1A can cooperate with AdE1B or activated ras genes to transform both human ( 6 ) and rodent ( 33 ) cells by targeting a limited number of cellular proteins through the N-terminal region, CR1, CR2, and CR4 ( 3 , 18 , 26 , 44 ). (asm.org)
  • In this report, we show that the IE1 and IE2 gene products can, indeed, cooperate with E1A to transform primary baby rat kidney (BRK) cells. (pnas.org)
  • CtBP
  • REFERENCE 3 (residues 1 to 445) AUTHORS Bellesis AG, Jecrois AM, Hayes JA, Schiffer CA and Royer WE Jr. TITLE Assembly of human C-terminal binding protein (CtBP) into tetramers JOURNAL J. Biol. (genome.jp)
  • molecular
  • Biochemical and molecular genetic explorations have defined a single tyrosine phosphorylation site and, in a dimeric partner molecule, an Src homology 2 (SH2) phosphotyrosine-binding domain, a DNA interaction domain, and a number of protein-protein interaction domains (with receptors, other transcription factors, the transcription machinery, and perhaps a tyrosine phosphatase). (sciencemag.org)
  • expression
  • For other proteins similar situations may exist, i.e. for certain uses the specific glycosylation pattern observed upon expression in El A-expressing cells may be highly beneficial, while for other purposes a different glycosylation profile may be more suitable. (allindianpatents.com)
  • In particular, the interplay and potential competition between the various glycosyltransferases and other actors in the glycosylation process in cells that express El A, rendered the outcome of over-expression of a sialyltransferase in such cells unforeseen and unpredictable in terms of glycosylation patterns of proteins thus produced. (allindianpatents.com)
  • We observed that the CMV promoter-mediated high levels of E1A expression could increase virus replication, resulting in the titers of the E1B55K-mutated virus being even higher than the wild-type virus in some cancer cells. (nih.gov)
  • Future efforts in the development of transcription-controlled oncolytic adenoviruses should focus on how to completely block E1a expression in normal cells. (nih.gov)
  • Induction of gene expression by exon 2 of the major E1A proteins of adenovirus type 5. (mcmaster.ca)
  • Disruption of the coordinate expression of muscle genes in a transfected BC3H1 myoblast cell line producing a low level of the adenovirus E1A transforming protein. (mcmaster.ca)
  • functional
  • A great deal of new information has accumulated about specificity in the activation of the STATs, about the functional domains of the proteins, and about the variety of their biologic functions both in development and in adults. (sciencemag.org)
  • viral
  • The protein products of 12S and 13S differ only in the presence of conserved region 3 (CR3), which functions to transactivate a number of cellular and viral genes ( 22 ). (asm.org)
  • We also observed that the virus without any promoter for the E1a gene could still express leaky levels of E1A which can lead to viral replication in normal and cancer cells. (nih.gov)
  • The viral immediate-early gene Quetiapine manufacture item E1A-12 may be the crucial determinant of tumorigenesis (38). (globaltechbiz.com)
  • In case of HIV-1 infection, it is recruited by the viral protein Tat. (proteopedia.org)
  • promoters
  • A variety of other E1A response elements have been identified in the E1A-stimulated hsp70, PCNA, and the adeno-associated virus P5 promoters ( 32 , 36 , 50 , 54 ). (asm.org)
  • mammalian
  • Mouse genetics experiments have defined crucial roles for each known mammalian STAT. The discovery of a STAT in Drosophila , and most recently in Dictyostelium discoideum , implies an ancient evolutionary origin for this dual-function set of proteins. (sciencemag.org)