Our results show that 2HF induced apoptosis in both histological types of lung cancer and inhibited proliferation and growth through suppression of CDK4, CCNB1, PIK3CA, AKT and RPS6KB1 (P70S6K) signaling. (oncotarget.com)
Using a cell apoptosis assay based on stimulation of a RANK:Fas fusion protein, and a cellular NF-kappaB signaling assay, we showed that R12-31 was agonist for both species. (cnrs.fr)
Tumor4
This protein shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro. (wikipedia.org)
Some of the new approaches depend on tumor biology and aim specifically to inhibit tumor growth and metastasis by targeting the tumor microenvironment or vasculature (leaving normal cells unaffected) or focusing on specific protein or signal transduction pathways. (medscape.com)
Here, we report, that the two E2F-binding sites in the adenoviral E2-early promoter contribute substantially to the so-called E1A-like activity in tumor cells. (bvsalud.org)
The role of YAP and TAZ in promoting tumor formation, progression, and resistance to therapy in lung cancer is emerging. (mdpi.com)
Histone7
BACKGROUND The histone methyltransferase (HMT) family includes histone lysine methyltransferases (HKMTs) and histone/protein arginine methyltransferases (PRMTs). (cancerindex.org)
Cell viability/proliferation assays, cell cycle analysis by flow cytometry, immunodetection of specific histone and p53 acetylation marks, western blotting, siRNA and RT-qPCR. (biomedcentral.com)
Although treatment with curcumin, garcinol or the garcinol derivative LTK-14 hampered MCF7 cell proliferation, differential effects of these compounds on histone modifications were observed. (biomedcentral.com)
In summary, although garcinol and curcumin can both inhibit histone acetyltransferase activities, our results show that these compounds have differential effects on cancer cells in culture. (biomedcentral.com)
Histone modifications act as signals that are 'read' by sensory proteins containing bromodomains, PHD fingers and other domains, many of which function as coregulators of DNA-binding transcription factors [ 4 ]. (biomedcentral.com)
A number of natural and synthetic molecules that inhibit histone acetyltransferase (HAT) or histone deacetylase (HDAC) activities have been described. (biomedcentral.com)
The function of histone modifying epigenetic regulators and noncoding RNA as central mediators of epithelial stem cell renewal and differentiation represent major emerging areas of study in the lab. (stanford.edu)
Viral4
The LxCxE motif binds to all pocket proteins and is present in chromatin regulators such as HDAC and KDM5A and in viral proteins. (eu.org)
It also exhaustively introduces the concrete mechanism of invading GC cells and the viral genome composition of adenovirus and herpes simplex virus type 1 (HSV-1). (frontiersin.org)
In this study, we investigated different cell cycle inhibitors with respect to their ability to increase viral DNA replication of dl70-3, an E1-deleted adenovirus. (bvsalud.org)
Enzymes that modify chromatin and associated proteins by the addition or removal of acetyl or methyl groups play a key role in genome regulation [ 1 ]. (biomedcentral.com)
The protein encoded by this gene is a mitogen-inducible monokine and is one of the major HIV-suppressive factors produced by CD8+ T-cells. (cancerindex.org)
Gene8
Protein CREG1 (Cellular Repressor of E1A-stimulated Genes 1) is a protein that in humans is encoded by the CREG1 gene. (wikipedia.org)
The adenovirus E1A protein both activates and represses gene expression to promote cellular proliferation and inhibit differentiation. (wikipedia.org)
This gene may contribute to the transcriptional control of cell growth and differentiation. (wikipedia.org)
What pathways are this gene/protein implicaed in? (cancerindex.org)
The other project involves the study of a single nucleotide polymorphism (SNP) in the human p53 gene that results in either arginine (R) or proline (P) at position 72 of the p53 protein. (mdanderson.org)
This process is mediated by 2 mutually exclusive programs of gene expression: 1) an undifferentiated program supporting proliferation by stem cells within the basal layer and 2) a differentiation program instructing growth arrest and differentiation-associated programmed cell death in suprabasal layers. (stanford.edu)
We are currently pursuing studies of the dominant signaling and gene regulatory networks that control this process, including the Ras/MAPK cascade, which is required for stem cell-mediated self-renewal and the p53 transcription factor family member, p63, which is required for epidermal differentiation. (stanford.edu)
Activation3
The adenovirus E1A protein mimics the LxDLFD motif and competes with E2F for binding to Rb, promoting E2F activation and cell proliferation. (eu.org)
As a consequence, the synthesis and release of Th1- (T helper 1) and Th2- (T helper 2) type cytokines, and other inflammatory mediators from T-cells and mast cells are blocked and the expression of signals essential for the activation of inflammatory T-lymphocytes is inhibited. (medindex.am)
yet, a global view of bcr activation-regulated protein ubiquitination is lacking when ebv is actively replicating. (liverpool.ac.uk)
Lineage2
This family, which includes epidermal growth factor receptor (EGFR), plays a pivotal role in normal cell growth, lineage determination, repair, and functional differentiation. (medscape.com)
chronic myeloid leukemia (cml) is characterized by increased and unregulated proliferation of granulocytic lineage in the bone marrow and presence of these immature myeloid cells in the peripheral blood with presence of philadelphia (ph) chromosome. (liverpool.ac.uk)
Molecules1
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Functional2
Pocket proteins play essential roles in cell cycle progression, quiescence and differentiation, and their functional disruption is associated with human cancer. (eu.org)
To benchmark Live-seq, we used cell growth, functional responses and whole-cell transcriptome read-outs to demonstrate that Live-seq can accurately stratify diverse cell types and states without inducing major cellular perturbations. (bvsalud.org)
Motif1
The protein contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. (cancerindex.org)
Growth3
We carried out mosaic analysis to elucidate the functions of dMyc in the germline and somatic cells of the ovary during oogenesis, a process that involves cell proliferation, differentiation and growth. (biologists.com)
In previous studies, we found that 2'-hydroxyflavonone (2HF), a citrus flavonoid, inhibits the growth of renal cell carcinoma in a VHL-dependent manner. (oncotarget.com)
Results of in-vivo nude mouse xenograft studies of SCLC and NSCLC, which showed that orally administered 2HF inhibited growth of both histological types of lung cancer, confirmed in-vitro study results. (oncotarget.com)
Inflammatory1
The encoded protein is secreted and has chemokinetic and inflammatory functions. (cancerindex.org)
Stimulation1
As a proof of concept, we show that Live-seq can be used to directly map a cell's trajectory by sequentially profiling the transcriptomes of individual macrophages before and after lipopolysaccharide (LPS) stimulation, and of adipose stromal cells pre- and post-differentiation. (bvsalud.org)
Progression2
Pocket proteins Rb, p107 and p130 play central roles in cell cycle progression and differentiation. (eu.org)
The most studied function of the Rb protein is in the regulation of cell cycle progression at the G1/S boundary ( Giacinti,2006 ). (eu.org)
Cells5
DNA replication of E1-deleted first-generation adenoviruses (AdV) in cultured cancer cells has been reported repeatedly and it was suggested that certain cellular proteins could functionally compensate for E1A, leading to the expression of the early region 2 (E2)-encoded proteins and subsequently virus replication. (bvsalud.org)
Thus, our data show that E2F-binding sites in the E2-early promoter are crucial for E1A independent adenoviral DNA replication of E1-deleted vectors in cancer cells. (bvsalud.org)
Additionally, 2HF inhibited efflux of doxorubicin and increased its accumulation in the cells, but did not add to the transport inhibitory effect of anti-Rlip antibodies alone. (oncotarget.com)
while this machinery was originally identified by its ability to allow cells to survive starvation via lysosomal degradation to recycle cellular components, it has recently become apparent that it also is used by cells to secrete cytoplasmic constituents. (liverpool.ac.uk)
In stratified epithelia proliferative basal cells adherent to the underlying basement membrane undergo cell cycle arrest then outward migration and terminal differentiation. (stanford.edu)
Activity1
Referring to this, the observation was named E1A-like activity. (bvsalud.org)
Molecular2
Dystrophin associates with a number of proteins to form a large oligomeric complex named the dystrophin-glycoprotein complex (DGC), which bridges across the sarcolemma and connects the extracellular matrix and the actin cytoskeleton (Allamand and Campbell, (2000) Human Molecular Genetics 9:2459-2467). (justia.com)
the discovery of the molecular machinery of autophagy, namely atg proteins, was awarded with the nobel prize in physiology and medicine to yoshinori ohsumi in 2016. (liverpool.ac.uk)
Position2
The conserved last acidic position D48 from E1A ( 2R7G ) makes extensive hydrogen bonds with the main chain of S644/T645 and the hydroxyl moiety of S644/S646. (eu.org)
The corresponding position in E2F1/2 (D428/429) faces the solvent and does not form interactions, suggesting one additional residue in helical conformation, as observed in E1A, is required for proper positioning of the last acidic residue. (eu.org)
Major1
At the cellular level, acquired resistance to the current therapies is a major obstacle. (mdpi.com)
Type1
Lomefloxacin hydrochloride inhibits DNA gyrase, a type II topoisomerase involved in the induction or relaxation of supercoiling during DNA replication. (medindex.am)
Levels1
Some new promising strategies for biomarker discovery include microarray-based profiling at the DNA and mRNA levels, and mass-spectrometry-based profiling at the protein and peptide levels [ 2 ]. (biomedcentral.com)
Role1
These models are being used to systematically elucidate proteins required for cutaneous carcinogenesis and to test their potential role as therapeutic targets. (stanford.edu)
Includes1
Scope includes mutations and abnormal protein expression. (cancerindex.org)