Adenosine dependent protein kinases. Medical search. Frequent questions

cAMP activates the cAMP-dependent protein kinase (PKA). (nih.gov)
We recently showed phosphorylation of Drosophila Plexin A by cyclic adenosine monophosphate (cAMP)-dependent protein kinase (PKA) and subsequent inhibition of plexin-mediated repulsive guidance. (stanford.edu)
Activation by cGMP plus MgATP was blocked by KT-5823, an inhibitor of PKG, but not by KT-5720, an inhibitor of cAMP-dependent protein kinase (PKA). (nebraska.edu)

Protein kinases possess a catalytic subunit which transfers the gamma phosphate from nucleoside triphosphates (almost always ATP) to the side chain of an amino acid in a protein, resulting in a conformational and/or dynamic changes affecting protein function. (wikipedia.org)
This gene provides instructions for making one part (subunit) of an enzyme called protein kinase A, which promotes cell growth and division (proliferation). (medlineplus.gov)
The subunit produced from the PRKAR1A gene, called type 1 alpha, helps control whether protein kinase A is turned on or off. (medlineplus.gov)
The lack of this subunit causes protein kinase A to be turned on more often than normal, which leads to uncontrolled cell proliferation. (medlineplus.gov)
Both of these compounds are thought to block PKA actions through competitive inhibition of the adenosine triphosphate site on the PKA catalytic subunit. (sinapse.ac.uk)
The catalytic subunit of adenosine 3',5'-cyclic monophosphate-dependent protein kinase (PKA, 200 nM, with 1 mM ATP) activates a channel with similar characteristics. (johnshopkins.edu)
Included under this category are two cyclic-AMP-dependent protein kinase subtypes, each of which is defined by its subunit composition. (rush.edu)

Cyclic adenosine 3',5'-monophosphate-dependent protein kinase of human erythrocyte membranes. (elsevierpure.com)
Phosphodiesterases (PDEs) regulate the local concentration of 3',5' cyclic adenosine monophosphate (cAMP) within cells. (nih.gov)
MTX increases intracellular accumulation of adenosine monophosphate (AMP) and 5-aminoimidazole-4-carboxamide ribonucleotide which activates AMP-activated protein kinase (AMPK). (bmj.com)
Recruitment of coactivator glucocorticoid receptor interacting protein 1 to an estrogen receptor transcription complex is regulated by the 3',5'-cyclic adenosine 5'-monophosphate-dependent protein kinase. (uib.no)
This effect is governed in part by the nitric oxide regulation of cyclic guanosine and adenosine monophosphate-dependent protein kinases (PKG and PKA) and is not fully understood. (cdc.gov)
Signaling through the cyclic adenosine monophosphate-dependent protein kinase [protein kinase A (PKA)] is an important and widely studied area of signal transduction research. (sinapse.ac.uk)
Additionally, influenza A virus infection in A549 cells induced 5' adenosine monophosphate-activated protein kinase (AMPK) phosphorylation and activation in a time-dependent manner and wogonin treatment led to the suppression of AMPK phosphorylation. (elis.sk)
The patch-clamp method was employed to establish the mechanism of regulation by guanosine 3',5'-cyclic monophosphate (cGMP)dependent protein kinase (PKG) of large Ca 2+ -activated K + channels (BK(Ca)) in human mesangial cells. (nebraska.edu)
Chloride channels at the apical membrane of intestinal epithelial cells are involved in the excessive fluid secretion in diarrhea and diminished secretion in cystic fibrosis (CF). Diarrhea induced by heat-stable toxin from Escherichia coli is associated with elevated guanosine 3',5'-cyclic monophosphate (cGMP) in intestinal epithelial cells, but it is unknown whether chloride secretion is regulated by cGMP directly or via cGMP- dependent protein kinase (PKG). (johnshopkins.edu)
In mammals, adenosine 3', 5'-cyclic monophosphate (cAMP) is known to play highly important roles in sperm motility and acrosomal exocytosis. (plos.org)
Cyclic AMP (adenosine 3', 5'-cyclic monophosphate) is an important player in sperm function. (plos.org)
As in somatic cells, sperm intracellular cAMP concentration is regulated by the opposite action of two enzymes: the adenylyl cyclases (AC), which synthesize cAMP from ATP, and the phosphodiesterases (PDE), which break the phosphodiester bond of cAMP to form 5'-adenosine monophosphate (AMP). (plos.org)
Some natural substances are able to prevent these developments by targeting low-density lipoprotein levels and enzymes involved in choleserol synthesis, such as 5'- adenosine monophosphate activated protein kinase (AMPK), and 3-Hydroxy-3-Methylglutaryl-CoA reductase (HMGCR). (naturalnews.com)
In addition, cyclic adenosine monophosphate response element-binding protein (CREB) was found to be activated by both longitudinal and transverse stretch. (escholarship.org)
AICAR or 5-Aminoimidazole-4-carboxamide ribonucleotide, is a synthetic adenosine monophosphate analog. (biotechpeptides.com)
Myeloid-specific ablation of LPS-induced cytidine monophosphate kinase 2 (CMPK2), which is rate limiting for mtDNA synthesis, reduced ARDS severity without a direct effect on IL-6. (lu.se)

Protein kinase function has been evolutionarily conserved from Escherichia coli to Homo sapiens. (wikipedia.org)

Cyclic AMP-dependent protein kinase (A-kinase) did not exert such an effect. (portlandpress.com)
Mechanistically, MTX treatment led to cyclic AMP response element-binding protein (CREB) Ser133 phosphorylation, while AMPK depletion attenuated this response and the induction of MnSOD and HO-1. (bmj.com)
Activator of protein kinase A (cyclic AMP agonist). (biolog.de)
Cyclic AMP-Dependent Protein Kinases" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (rush.edu)
A group of enzymes that are dependent on CYCLIC AMP and catalyze the phosphorylation of SERINE or THREONINE residues on proteins. (rush.edu)
This graph shows the total number of publications written about "Cyclic AMP-Dependent Protein Kinases" by people in this website by year, and whether "Cyclic AMP-Dependent Protein Kinases" was a major or minor topic of these publications. (rush.edu)
Below are the most recent publications written about "Cyclic AMP-Dependent Protein Kinases" by people in Profiles. (rush.edu)

The catalytic subunits of protein kinases are highly conserved, and the structures of over 280 of the approximately 500 human kinase domains have been determined, leading to large screens to develop kinase-specific inhibitors for the treatments of a number of diseases. (wikipedia.org)
The increase in 36Cl- permeability is diminished by protein kinase A inhibitors and is not mediated by an increase in intracellular calcium concentrations. (nih.gov)
Protein regulators of this enzyme include inhibitors I2 (PPP1R2) and I3 (PPP1R11), sds22 (PPP1R7), protein 14-3-3 and a protein kinase, glycogen synthase kinase 3 (GSK-3). (kent.edu)
The response to GLP-1 was mimicked by forskolin and largely inhibited by the protein kinase A (PKA) inhibitors, H89 and myristoylated PKI(14-22) amide, indicating partial mediation via a cAMP/PKA pathway. (diabetesjournals.org)
Our data characterize phosp27-CDK4-CycD1 as an active Rb kinase that is refractory to clinically relevant CDK4/6 inhibitors. (rcsb.org)

Puri P, Myers K, Kline D, Vijayaraghavan S. Proteomic amalysis of bovine sperm YWHA binding partners identify proteins involved in signaling and metabolism. (kent.edu)
Glucose metabolism activates this pathway by means of increasing [Ca 2+ ] i via L-type voltage-dependent calcium channels (VDCCs) by affecting the electrical activity of the cell. (diabetesjournals.org)
Adenosine functions as a critical ligand, after binding to membrane-associated adenosine receptors, adenosine initiates a downstream signaling cascade and subsequently contributes to functions of nervous system, immune response, vascular function and even metabolism. (tmc.edu)

Phospholipase D activity in human platelets is inhibited by protein kinase A, involving inhibition of phospholipase D1 translocation. (uib.no)
It was observed that yuzu enables a dose-dependent inhibition of lipid accumulation of up to 23 percent. (naturalnews.com)
As binding of miRNAs to a target mRNA may result in an airway inflammatory disease closely correlates with either mRNA degradation or inhibition of protein immune regulation, more and more evidences suggest translation.1 More than 2000 miRNAs have been discovered in humans. (cdc.gov)

Structural and biochemical data revealed that binding of phosphorylated p27 (phosp27) to CDK4 altered the kinase adenosine triphosphate site to promote phosphorylation of the retinoblastoma tumor suppressor protein (Rb) and other substrates. (rcsb.org)

More importantly, we found that IPMK interacted with AMPK and functioned as a positive regulator of AMPK protein in osteosarcoma cells. (ijbs.com)
A rescue study showed that the induction of autophagy and activation of the AMPK/ULK1 signaling pathway by ebastine treatment were reversed by IPMK knockdown, indicating that the activity of ebastine was IPMK dependent. (ijbs.com)
We provide experimental evidence demonstrating that ebastine has antitumor activity in osteosarcoma and promotes autophagy by activating the AMPK/ULK1 signaling pathway, which is IPMK dependent. (ijbs.com)
It was developed to stimulate the AMP-dependent protein kinase (AMPK) activity. (biotechpeptides.com)
The hearts were perfused in the absence or presence of AICAR, an adenosine analog suggested to activate AMPK and potentially penetrate cells. (biotechpeptides.com)
By targeting electron transport chain complex 1 and independently of AMP-activated protein kinase (AMPK) or NF-κB, metformin blocked LPS-induced and ATP-dependent mitochondrial (mt) DNA synthesis and generation of oxidized mtDNA, an NLRP3 ligand. (lu.se)

Many heterologously expressed mutants of the cystic fibrosis transmembrane conductance regulator (CFTR) exhibit residual chloride channel activity that can be stimulated by agonists of the adenylate cyclase/protein kinase A pathway. (nih.gov)
Use of polarized PC12 cells to monitor protein localization in the early biosynthetic pathway. (uib.no)
Western blot was carried out to detect the expression levels of autophagic-related proteins (Unc-51 like autophagy activating kinase 1, beclin 1, autophagy related 5, microtubule-associated protein 1A/1B-light chain 3, ubiquitin-binding protein p62), glycolysis-related proteins (hexokinase 2, phosphofructokinase, pyruvate kinase M2) and activated protein kinase signalling pathway proteins. (ijpsonline.com)
It has been reported that lung cancer cells exhibit upregulated expression of all key glycolytic enzymes [hexokinase 2 (HK2), phosphofructokinase and pyruvate kinase (PK)] ( 13 ), suggesting that the essential enzymes of the aerobic glycolytic pathway have a critical role in the development of lung carcinoma. (spandidos-publications.com)

We found that p27, when phosphorylated by tyrosine kinases, allosterically activated CDK4 in complex with cyclin D1 (CDK4-CycD1). (rcsb.org)

In addition to its PRKA-dependent effects, different studies have clearly demonstrated that cAMP also activates EPAC ( E xchange P roteins directly A ctivated by c AMP) during capacitation and acrosome exocytosis [ 15 - 17 ]. (plos.org)

Eukaryotic protein kinases are enzymes that belong to a very extensive family of proteins which share a conserved catalytic core common with both serine/threonine and tyrosine protein kinases. (wikipedia.org)
This phosphorylation occurs in the active site of the plexin GTPase-activating protein (GAP) domain, which in turn inhibits endogenous GAP activity toward Ras/Rap family small GTP-binding proteins by recruiting the phospho-serine/threonine-binding protein 14-3-3ε. (stanford.edu)

Since adenosine A 2A receptors (A 2A Rs) control lysosome trafficking and pH, which closely regulates lysosomal calcium, we hypothesized a role for these receptors in NPC1. (jneurosci.org)
Although adenosine signaling is beneficial under stress conditions in various organs and tissues via activation of different adenosine receptors, its significance and functional role in the erythroid lineage, particularly under stress conditions, are still unclear. (tmc.edu)

ROS may induce intracellular calcium increase and subsequent contraction of PASMCs via direct or indirect interactions with protein kinases, phospholipases, sarcoplasmic calcium channels, transient receptor potential channels, voltage-dependent potassium channels and L-type calcium channels, whose relevance may vary under different experimental conditions. (ersjournals.com)
Huang Z, Somanath PR, Chakrabarti R, Eddy EM, Vijayaraghavan S. Changes in intracellular distribution and activity of protein phosphatase PP1gamma2 and its regulating proteins in spermatozoa lacking AKAP4. (kent.edu)
Significantly, the actions of both GLP-1 and forskolin were abolished by the selective Ca 2+ /calmodulin-dependent phosphatase 2B (calcineurin) inhibitor, FK506, as well as by the chelation of intracellular Ca 2+ by BAPTA (bis(2-aminophenoxy)ethane- N,N,N ′ ,N ′-tetraacetate). (diabetesjournals.org)

These data indicate that endogenous levels of deltaF508 protein can be stimulated to increase 36Cl- permeability in airway epithelial cells. (nih.gov)

Protein kinases are a group of enzymes that move a phosphate group onto proteins, in a process called phosphorylation. (wikipedia.org)

Using gene knockout, transgenic and molecular approaches we are investigating the signal mechanisms underlying regulation of protein phosphorylation and sperm function. (kent.edu)
Vijayaraghavan S, Chakrabarti R, Myers K. Regulation of sperm function by protein phosphatase PP1gamma2. (kent.edu)
2) Adenosine signaling via erythroid ADORA2B is a previously unrecognized purinergic signaling responsible for stress erythropoiesis by regulation of erythroid commitment in a hypoxia-inducible factor 1-alpha dependent manner. (tmc.edu)
Thus, my research has identified that adenosine signaling functions as a novel molecular mechanism involved in the regulation of erythrocyte O 2 release capacity and O 2 delivery capacity, providing therapeutic possibilities to enhance O 2 availability and prevent stress-induced tissue damage and inflammation, a strong foundation for future clinical trials to treat diseases associated with hypoxia or hematology disorders provided by my doctoral work. (tmc.edu)

Further in vivo experiments employed a mouse model of P. aeruginosa acute pneumonia to verify the target proteins and signaling pathways affected by JFG, which were predicted by the network pharmacology analysis. (bvsalud.org)

We hypothesized that helium induces pre- and postconditioning in CABG-patients, affecting signaling molecules protein kinase C-epsilon (PKC-ε), p38 mitogen activated protein kinase (p38 MAPK), extracellular signal-regulated kinase 1/2 (ERK-1/2) and heat shock protein 27 (HSP-27) within cardiac tissue, and reducing postoperative troponin levels. (biomedcentral.com)

[email protected] Abstract Orientation-dependent reactivity and band-bending are evidenced upon Ti deposition (1-10 Å) on the polar ZnO(0001)-Zn and ZnO(000 1 )-O surfaces. (fdocuments.fr)

they include actions on other protein kinases and signaling molecules and also on basic cellular functions, such as transcription. (sinapse.ac.uk)

Here we describe how phosphorylation of Plexin A can be detected and quantitated using an in vitro kinase assay and radioactive [γ-P32] adenosine 5'-triphosphate (ATP). (stanford.edu)

The protein kinase domain is a structurally conserved protein domain containing the catalytic function of protein kinases. (wikipedia.org)

This protein phosphatase isoform, which is highly expressed in testis, is present only in mammalian spermatozoa. (kent.edu)
Dudiki T, Joudeh N, Sinha N, Goswami S, Eisa A, Kline D, Vijayaraghavan S. The protein phosphatase isoform PP1γ1 substitutes for PP1γ2 to support spermatogenesis but not normal sperm function and fertility. (kent.edu)
Chakrabarti R, Cheng L, Puri P, Soler D, Vijayaraghavan S. Protein phosphatase PP1 gamma 2 in sperm morphogenesis and epididymal initiation of sperm motility. (kent.edu)
We have previously identified NFAT (nuclear factor of activated T-cells) as a key regulator of insulin gene transcription in pancreatic β-cells that is activated by the calcium/calmodulin-dependent protein phosphatase 2B (calcineurin) in response to increased [Ca 2+ ] i ( 10 ). (diabetesjournals.org)

The AMP-activated protein kinase is an enzyme and a protein that may play a regulatory role in several metabolic pathways. (biotechpeptides.com)

Phosphorylation usually results in a functional change of the target protein by changing structure, dynamics, enzyme activity, cellular location, or association with other proteins. (wikipedia.org)
Protein phosphorylation, which plays a key role in most cellular activities, is a reversible process mediated by protein kinases and phosphoprotein phosphatases. (embl.de)
This noble gas induced cardioprotective effect was abolished on a cellular level by blockers of protein kinase C (PKC) and p38 mitogen activated protein kinase (p38 MAPK) [ 3 ]. (biomedcentral.com)

Helium preconditioning did not significantly alter the primary outcome (molecular levels of kinases PKC-ε and HSP-27, ratio of activated p38 MAPK or ERK ½). (biomedcentral.com)

The effect of phosphorylation by cyclic GMP-dependent protein kinase (G-kinase) on the activity of the plasmalemmal Ca2+-transport ATPase was studied on isolated plasma membranes and on the ATPase purified from pig erythrocytes and from the smooth muscle of pig stomach and pig aorta. (portlandpress.com)

G-kinase, but not A-kinase, stimulated the phosphorylation of PI to phosphatidylinositol phosphate (PIP) in a preparation of (Ca2+ + Mg2+)-ATPase obtained by calmodulin affinity chromatography from smooth muscle, but not in a similar preparation from erythrocytes. (portlandpress.com)
Protein kinases catalyse the transfer of the gamma phosphate from nucleotide triphosphates (often ATP) to one or more amino acid residues in a protein substrate side chain, resulting in a conformational change affecting protein function. (embl.de)

Dual specificity protein kinases (e.g. (embl.de)

These data suggest that the synergistic action of glucose and GLP-1 to promote insulin gene transcription is mediated through NFAT via PKA- and calcineurin-dependent pathways in pancreatic β-cells. (diabetesjournals.org)
Hypoxia and genetic defects that chronically drive proliferation leave such tumors dependent on a steady supply of nutrients, especially glucose. (springer.com)

The mechanism may be related to the improvement of glycolysis dysfunction and alleviation of autophagy over activation of activated protein kinase. (ijpsonline.com)
Autophagy is a process that relies on lysosomal pathways for the degradation of cytoplasmic proteins and organelles and plays an important role in the pathology of brain injury such as hypoxia ischemia[ 3 ]. (ijpsonline.com)

The stimulation of the (Ca2+ + Mg2+)-ATPase activity by G-kinase was only observed in the presence of phosphatidylinositol (PI). (portlandpress.com)

11. Amsel, A. & Roussel, J. Motivational properties of ences in post-transcriptional processes spark interest in the development of frustration: I. Effect on a running response of the (protein redistribution, degradation), pharmacotherapies that selectively reg- addition of frustration to the motivational com- plex. (lu.se)

The stimulation of the (Ca2+ + Mg2+)-dependent ATPase activity of the purified Ca2+ pump reconstituted in liposomes depended on the phospholipid used for reconstitution. (portlandpress.com)
The proliferation activity, mortality of SH-SY5Y cells, adenosine triphosphate and pyruvic acid were measured. (ijpsonline.com)
Analysis of this network identified serum response factor (SRF) and myocyte enhancer factor-2 (MEF2) as critical TFs in regulating longitudinal stretch-induced gene changes whose activity is modulated by protein kinase C (PKC). (escholarship.org)
formate import into anaerobic E. coli cells as determined by the enhanced activity of a single-copy formate-dependent fdhF∷lacZ fusion. (agckinases.com)

By targeting electron transport chain complex 1 and independently of AMP-activated protein kinase. (lu.se)

The 18-kDa translocator protein (TSPO) is increasingly recognized as a molecular target for PET imaging of inflammatory responses in various central nervous system (CNS) disorders. (iasp-pain.org)

Adenosine inhibited both the phosphorylation of PI and the stimulation of the (Ca2+ + Mg2+)-ATPase by G-kinase. (portlandpress.com)

My dissertation employing multi-disciplinary approaches including human studies, novel genetic tools, and sophisticated pharmacological studies coupled with metabolomic profiling shows for the first time that 1) CD73-dependent elevation of plasma adenosine signaling via erythrocyte specific ADORA2B coupled with AMP-activated protein kinase is a key mechanism for hypoxia adaptation via induction of 2,3-BPG production with O 2 release to counteract severe hypoxia. (tmc.edu)

The p27 protein is a canonical negative regulator of cell proliferation and acts primarily by inhibiting cyclin-dependent kinases (CDKs). (rcsb.org)

Snow AJ, Puri P, Acker-Palmer A, Bouwmeester T, Vijayaraghavan S, Kline D. Phosphorylation-dependent interaction of tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein (YWHA) with PADI6 following oocyte maturation in mice.Biol Reprod. (kent.edu)
The first one is located in an activation loop in the centre of the kinase domain. (embl.de)
Stockand, JD & Sansom, SC 1996, ' Mechanism of activation by cGMP-dependent protein kinase of large Ca 2+ -activated K + channels in mesangial cells ', American Journal of Physiology - Cell Physiology , vol. 271, no. 5 40-5, pp. (nebraska.edu)
It is known to act through protein phosphorylation via PRKA and through the activation of guanine nucleotide exchange factors like EPAC. (plos.org)

Identification of a novel phosphorylation site of acyl-CoA binding protein (ACBP) in nodularin-induced apoptotic hepatocytes. (uib.no)

Yet it is unclear whether Gαi and Gαo operate through identical mechanisms and how these G-protein-mediated signals inhibit insulin secretion in vivo. (diabetesjournals.org)

Nogo-B is an endoplasmic reticulum (ER)-localized protein mediating ER-mitochondrial morphology. (bvsalud.org)

2008). Abolition of stress-induced protein synthesis sensitizes leukemia cells to anthracycline-induced death. (uib.no)

It is able to inhibit lipid accumulation by stimulating PPAR-alpha, CPT1, and beta-oxidation-related proteins and reducing HMGCR proteins. (naturalnews.com)

Although a high level of PDE10A gene expression is observed in the testis, information on the identity of the isoforms or on the cell type that express the PDE10 protein is lacking. (plos.org)
Although more and animals and plants and regulate gene/protein expression more progressions have been made about the through direct complementarity between their 5' region pathogenesis of asthma in recent years, the increasing and the 3' untranslated region of target mRNAs. (cdc.gov)

Anatomy19

Organisms11

Chemicals and Drugs172

Analytical, Diagnostic and Therapeutic Techniques and Equipment11

Phenomena and Processes39

Information Science3

CAMP-depend3

Subunit7

Monophosphate16

Evolutionarily conserved from Escherichia coli1

Cyclic AMP7

Inhibitors5

Metabolism3

Inhibition3

Triphosphate site1

AMPK6

Pathway4

Tyrosine kinases1

Activates1

Serine2

Receptors2

Intracellular3

Endogenous1

Enzymes1

Regulation4

Target proteins1

Induces1

Abstract1

Signaling molecules1

Assay1

Catalytic1

Phosphatase4

Metabolic pathways1

Cellular3

MAPK1

Membranes1

Phosphate2

Specificity1

Glucose2

Autophagy2

Phosphatidylinositol1

Degradation1

Activity4

COMPLEX1

Molecular1

Stimulation1

Human1

Proliferation1

Activation4

Phosphorylation site1

Insulin Secretion1

Mitochondrial1

Synthesis1

Accumulation1

Gene2