• Six diatom species displayed specific anti-inflammatory, anticancer (blocking human melanoma cell proliferation), and anti-biofilm (against the bacteria Staphylococcus epidermidis ) activities whereas, none of the other microalgae were bioactive against the conditions tested for. (frontiersin.org)
  • Overexpression of SLAMF1 or SLAMF7 in IGHV mutated CLL cell models resulted in reduced proliferation and impaired responses to BCR ligation, whereas the knockout of both receptors showed opposing effects and increased sensitivity toward inhibition of components of the BCR pathway. (nature.com)
  • B cell receptor (BCR) signaling plays a critical role in driving proliferation and survival of the malignant clone in chronic lymphocytic leukemia (CLL), supported by the clinical activity of inhibitors targeted toward BCR-associated kinases [ 1 ]. (nature.com)
  • Between clonal expansion and cell proliferation, the survival of infected cells requires the silencing of viral expression before the immune response. (biomedcentral.com)
  • miRNA-149 was underexpressed in gastric cancer tissues and cells, while overexpression of miRNA-149 promoted cell apoptosis, retarded cell cycle, and inhibited proliferation and migration in AGS and MKN28 cells. (hindawi.com)
  • It possesses a unique forkhead domain combined with a fragment of the target gene to initiate transcription [ 10 ] and regulates various biological processes such as tumor cell differentiation, cell proliferation, and migration [ 11 ]. (hindawi.com)
  • Kay et al report increased VEGF and bFGF in the supernatant of CLL cells grown in vitro and upregulation of mRNA encoding VEGF and its receptors and bFGF, suggesting that angiogenic factors are important in the biology of the malignant B-cell clone [ 10 ]. (biomedcentral.com)
  • The spontaneous immortalization of primary malignant cells is frequently assigned to their genetic instability during in vitro culturing. (biomedcentral.com)
  • The in vitro culture was established with cells isolated from ascites of a 60-year-old female patient with recurrent ovarian cancer. (biomedcentral.com)
  • This study demonstrates in vitro that certain cell types from pleomorphic adenoma are able to clone and survive over a long term and develop subcutaneous tumors in nude mice. (biomedcentral.com)
  • The use of human telomerase reverse transcriptase-immortalized bone marrow mesenchymal stromal cells (hTERT-BMSCs) as vehicles to deliver antinociceptive galanin (GAL) molecules into pain-processing centers represents a novel cell therapy strategy for pain management. (hindawi.com)
  • The use of cell lines as "biological minipumps" to chronically deliver antinociceptive molecules into the pain-processing centers of the spinal cord represents a newly developed technique for the treatment of pain [ 3 ]. (hindawi.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • A previous study demonstrated that SLAMF1, SLAMF2, and SLAMF7 receptors are rather downregulated on CLL cells as compared to their normal B cell counterpart [ 12 ], suggesting that high expression of these molecules may have detrimental (e.g., antiproliferative) effects in the CLL context. (nature.com)
  • The CAISMOV24 line was assessed for cell growth, production of soluble biomarkers, expression of surface molecules and screened for typical mutations found in serous ovarian carcinoma. (biomedcentral.com)
  • The cell surface molecules EpCAM, PVR and CD73 are overexpressed on CAISMOV24 cells compared to the primary malignant cells. (biomedcentral.com)
  • Moreover, treatment with imiquimod downregulated MHC class I molecules on tumor cells possibly resulting in a reduced binding affinity for inhibitory receptor NKG2A expressed on γδ T cells. (aacrjournals.org)
  • Transplanted cells in nude mice formed subcutaneous tumors, which had histological features of squamous cell carcinoma with apparent keratinizing tendencies. (biomedcentral.com)
  • It is reported that FOXC1 plays an important role in the occurrence, development, and migration of malignant tumors [ 12 ]. (hindawi.com)
  • This "angiogenic switch" [ 1 ] favors the production of new microvessels, thus facilitating tumor growth beyond 1-2 mm diameter, and metastasis of the malignant clone. (biomedcentral.com)
  • For the functional screening, Bcl2 - and Myc- expressing primary B cells were infected with a retroviral cDNA library. (haematologica.org)
  • Inserted cDNA of transformed cells in culture were then identified. (haematologica.org)
  • Purification, cDNA cloning, and gene mapping of the small subunit of human DNA polymerase epsilon. (nih.gov)
  • Genetic alterations of BCL2 (B-cell CLL/lymphoma 2), MYC [v-myc myelocytomatosis viral oncogene homolog (avian)] and CCND1 (cyclin D1) are the most frequently found alterations in human B-cell lymphomas. (haematologica.org)
  • About 4% of cases of diffuse large B-cell cell lymphoma possess BCL2/MYC double translocations, suggesting a synergistic effect of BCL2 and MYC in lymphoma development. (haematologica.org)
  • 8 - 12 Cases of CCND1/MYC double translocation are relatively frequent in mantle cell lymphoma. (haematologica.org)
  • Importantly, human B-cell lymphoma cases with concurrent multiple translocations including BCL2 , MYC and CCND1 have been reported. (haematologica.org)
  • Bovine leukemia virus (BLV) infects cattle, integrates into host DNA as a provirus, and induces malignant B-cell lymphoma. (biomedcentral.com)
  • Less than 5% of cattle develop B-cell leukemia/lymphoma after a prolonged latency period [ 1 ]. (biomedcentral.com)
  • A stage II trial of AUY922 can be open up in GIST [98] and a stage II trial has been approved but is not yet active for individuals with diffuse large B-cell lymphoma or peripheral T-cell lymphoma [99]. (forgetmenotinitiative.org)
  • Kyowa Hakko Kirin Pharma has developed KW-2478 which has been evaluated inside a phase I dose escalation trial in CLL, relaspsed/refractory multiple myeloma, and B-cell non-Hodgkin's lymphoma, and is in a phase I/II study in combination with bortezomib in relapsed/refractory multiple myeloma [101]. (forgetmenotinitiative.org)
  • Cutaneous T-cell lymphoma (CTCL) (see the image below) is a heterogeneous group of lymphoproliferative disorders characterized by localization of neoplastic T lymphocytes to the skin, with no evidence of extracutaneous disease at the time of diagnosis. (medscape.com)
  • Early patch-stage cutaneous T-cell lymphoma. (medscape.com)
  • Related articles include Cutaneous B-Cell Lymphoma and Cutaneous Pseudolymphoma . (medscape.com)
  • Among the changes to CTCL classification were the addition of primary cutaneous acral CD8 + T-cell lymphoma as a new provisional entity. (medscape.com)
  • Also, the term "primary cutaneous CD4 + small/medium T-cell lymphoma" was changed to "primary cutaneous CD4 + small/medium T-cell lymphoproliferative disorder" because of its indolent clinical behavior and uncertain malignant potential. (medscape.com)
  • The outcome for T-cell acute lymphoblastic leukemia (T-ALL) in relapse after hematopoietic stem cell transplantation (HSCT) is quite poor, while, both donor lymphocytes infusion (DLI) and adoptively infusion of γδ T cells in leukemia patients after HSCT have demonstrated good results in prolonging survival time of patients. (psu.edu)
  • Studies have increasingly focused on the potential therapeutic effects of stem cell transplantation for neurological diseases [ 10 ]. (hindawi.com)
  • We examined TLR3- and TLR7-expressing pancreatic adenocarcinomas, squamous cell carcinomas of head and neck and lung carcinomas. (aacrjournals.org)
  • SM-AP1-3 cells were immunopositive for keratin only, indicating their duct-epithelial or squamous cell differentiation, while SM-AP4/5 cells were positive for both keratin and S-100 protein, indicating their myoepithelial cell differentiation. (biomedcentral.com)
  • The histological features of squamous cell carcinoma from the transplanted cell systems in nude mice might suggest a secondary onset of malignancy from a pre-existing benign adenoma. (biomedcentral.com)
  • Indeed, the potent pathotropic migratory properties of BMSCs and ability to circumvent both the complications associated with immune rejection of allogenic cells and many of the moral reasons associated with embryonic stem cell use suggest that BMSCs are most promising stem cells as a potential target for the clinical use of genetically engineered stem cells [ 14 , 15 ]. (hindawi.com)
  • 28 from 3 embryonic stem [Ha sido] clones) sent the Ha sido cell genome. (tech-strategy.org)
  • It is a clonal genetic heterogeneous disease generally thought to arise from the malignant transformation and expansion of a single lymphoid progenitor at various stages of development ( 3 - 5 ). (frontiersin.org)
  • Although pathologists in their daily services of surgical pathology had recognized such singular atypical cells in pleomorphic adenomas, these atypical cells were not always regarded as evidence or the source for malignant transformation [ 4 - 6 ]. (biomedcentral.com)
  • Indeed, our experiments showed significantly decreased degranulation capacity of primary NK cells from CLL patients expressing low levels of SLAMF1 and SLAMF7. (nature.com)
  • The maximum number of evolved clones was significantly higher in patients with t (12;21)/ ETV6:RUNX1 . (frontiersin.org)
  • In addition, an identical Vd4 monoclonal T cell that proliferated in the recipient for several years was confirmed to come from the donor graft, and its expression level significantly increased in third leukemia recurrence. (psu.edu)
  • The conditioned media of the malignant II-4RT cells contain significantly more MMP-1 and MMP-8 than those of HaCaT or A5 as evidenced by immunoblotting and ELISA. (uni-bielefeld.de)
  • Bone marrow stem cells, including the pluripotent hematopoietic stem cells (HSCs) and bone mesenchymal stem cells (BMSCs), are being considered as potential targets for cell and gene therapy-based approaches against a variety of different diseases. (hindawi.com)
  • The objective of this study was to identify additional cooperative gene(s) associated with BCL2 and MYC or MYC and CCND1 in human B-cell lymphomagenesis. (haematologica.org)
  • In addition, they all had a common deletion of the last base G of codon 249 (AGG to AG_) of the p53 gene, which resulted in generation of its nonsense gene product. (biomedcentral.com)
  • We investigated cells and conditioned media of the three human keratinocyte cell lines HaCaT (non-tumorigenic), A5 (benign, tumorigenic) and II-4RT (malignant, tumorigenic) with regard to production and secretion of the collagenases-1 to -3 (MMP-1, MMP-8 and MMP-13) and TIMP-1 using semi-nested RT-PCR, Western blots, ELISA, immunocytochemistry and casein zymography. (uni-bielefeld.de)
  • It is still poorly understood how and which benign pleomorphic adenoma cells transform into its malignant form, carcinoma ex pleomorphic adenoma. (biomedcentral.com)
  • We have established five cell systems from a benign pleomorphic adenoma of the parotid gland of a 61-year-old woman. (biomedcentral.com)
  • We established and characterized five cell systems (designated as SM-AP1 to SM-AP5) from a benign pleomorphic adenoma of the parotid gland. (biomedcentral.com)
  • Since the SLAMF low signature was strongly associated with an unmutated CLL immunoglobulin heavy chain (IGHV) status in large datasets, we investigated the impact of SLAMF1 and SLAMF7 on the B cell receptor (BCR) signaling axis. (nature.com)
  • CD3 and CD3 are two highly related components of the T cell receptor (TCR)CCD3 complex which is essential for the assembly and transmission transduction of the T cell receptor on mature T cells. (tech-strategy.org)
  • The precise pathogenetic events leading to the development of ALL are still unknown, but evidence supports the hypothesis of driver mutations followed by secondary events, that can occur in subclones of the original leukemic cell following different evolution patterns ( 5 , 6 ). (frontiersin.org)
  • Based on our previous identification of a leukemic and reactive clone in this patient, continual γδ T cell repertoire monitoring affirmed that the same Vd5 leukemic clone existed in most samples from the patient, particularly including a sample taken at the time of the third T-ALL relapse, while it could not be detected in the donor sample. (psu.edu)
  • In this study, the new epithelial ovarian cancer cell line CAISMOV24 was described and compared with its original low-grade serous ovarian carcinoma. (biomedcentral.com)
  • This study clearly indicates that the epithelial tumor cells synthesize different collagenases and TIMP-1. (uni-bielefeld.de)
  • The human gastric epithelial permanent cell line (GES-1), human gastric cancer cell lines (AGS, BGC-823, MKN45, and SGC-7901), and HEK293 cells were provided by the Biomedical Experiment Center of Xi'an Jiaotong University. (hindawi.com)
  • Here, we investigated the modulation of γδ T cell-mediated tumor cell lysis by TLR ligands. (aacrjournals.org)
  • Although human HSCs as vehicles to treat metachromatic leukodystrophy (MLD) has been used to treat patients with early onset MLD in a phase I/II trial, the HSCs give rise to all different blood cell lineages, such as the myeloid and lymphoid cell lineages [ 11 ]. (hindawi.com)
  • Used together, these scholarly research showed that Compact disc3 and Compact disc3 play an important, yet overlapping partially, role in the introduction of both and T cell CH5424802 biological activity lineages. (tech-strategy.org)
  • High-throughput sequencing (HTS) of the immunoglobulin heavy chain ( IgH ) locus is a recent very efficient technique to monitor minimal residual disease of B-cell precursor acute lymphoblastic leukemia (BCP-ALL). (frontiersin.org)
  • B-cell precursor acute lymphoblastic leukemia (BCP-ALL) is the most common pediatric neoplasm ( 1 , 2 ). (frontiersin.org)
  • Results Primary B cells exogenously expressing Bcl2 , Myc and Ccnd1 showed factor-independent growth ability, enhanced colony-forming capability and aggressive oncogenicity, unlike the cases observed with the expression of any combination of only two of the genes. (haematologica.org)
  • Conclusions Bcl2 , Myc and Ccnd1 or Bcl2 , Myc and CCND3 synergistically transformed mouse primary B cells into aggressive malignant cells. (haematologica.org)
  • Since SLAMF receptors play a role in NK cell biology, we reasoned that these receptors may impact NK cell-mediated CLL immunity. (nature.com)
  • In the case of the infectious cycle, viruses reach target lymphocytes by cell-to-cell transfer and establish by entry of viral single-stranded RNA, reverse transcription of the viral RNA, integration as a provirus into the host genome, expression of viral proteins, and budding of new virions. (biomedcentral.com)
  • One cell suspensions of thymocytes, LN cells, spleen cells, PBL, and little intestine intraepithelial lymphocytes (iIEL) had been prepared as defined (26, 27). (tech-strategy.org)
  • Background A synergistic effect resulting from a combination of BCL2 and MYC or MYC and CCND1 has been implicated in human B-cell lymphomas. (haematologica.org)
  • 7 Importantly, it is believed that other hitherto unknown genes also play important roles in lymphomagenesis in addition to the synergistic effects of the aforementioned two oncogenes since human B-cell lymphomas often show a variety of genes subject to alterations and/or deregulated expression. (haematologica.org)
  • In our previous study, we proposed a concept of focal carcinomas in pleomorphic adenoma which is an advanced stage of accumulated atypical cells with P53 over-expressions as an initial stage or a latent form of apparent carcinomas secondarily arising in pleomorphic adenoma [ 3 ]. (biomedcentral.com)
  • Human gastric cancer cell lines AGS and MKN28 were cultured and transfected with miR-149 overexpression plasmid and its control or FOXC1 siRNA and its control. (hindawi.com)
  • However, obtaining primary neuronal cells from adult tissue is difficult and faces major ethical issues in clinical practice. (hindawi.com)
  • Its lethality is a consequence of the lack of symptoms or biomarkers enabling the early diagnosis of disease, and the propensity of malignant cells to seed the peritoneal cavity. (biomedcentral.com)
  • 2. A higher number of evolved clones at diagnosis of BCP-ALL was associated with the presence of t (12;21)/ ETV6:RUNX1 . (frontiersin.org)
  • Additionally, comparative genomic hybridization was employed to compare genomic alterations between the CAISMOV24 cell line and its primary malignant cells. (biomedcentral.com)
  • Although the cell line had developed alongside the accumulation of genomic alterations (28 CNV in primary cells and 37 CNV in CAISMOV24), most of them were related to CNVs already present in primary malignant cells. (biomedcentral.com)
  • Additionally, CAISMOV24 cell line was characterized as a low-grade serous ovarian carcinoma, which still resembles its primary malignant cells. (biomedcentral.com)
  • However, cell lines are known for their susceptibility to genetic and metabolic alterations, which can alter their characteristics in relation to the primary malignant cells. (biomedcentral.com)
  • Ideally, the more a cell line resembles the primary malignant cells, the more useful it will be for scientific research. (biomedcentral.com)
  • Design and Methods Growth in culture, colony formation and oncogenicity in vivo were assessed in mouse primary B cells exogenously expressing various combinations of Bcl2 , Myc and Ccnd1 . (haematologica.org)
  • Two indicators of clonal complexity were used, namely, the number of foster clones, described as clones with similar D-N 2 -J rearrangements but other V-rearrangement and N 1 -joining, and the maximum across all foster clones of the number of evolved clones from one foster clone. (frontiersin.org)
  • Here, we collected blood samples from a single, naturally infected Holstein cow at three disease progression stages (Stage I: polyclonal stage, Stage II: polyclonal toward oligoclonal stage, Stage III: oligoclonal stage) and successfully visualized the kinetics of clonal expansion of cells carrying BLV integration sites using our BLV proviral DNA-capture sequencing method. (biomedcentral.com)
  • Our results are the first to demonstrate clonal expansion after the massive depletion of cells carrying BLV integration sites in a naturally infected cow. (biomedcentral.com)
  • this limitation has been overcome via ectopic expression of human telomerase reverse transcriptase (hTERT), the catalytic component of telomerase, to produce large quantities of these cells as an attractive source for cellular transplantation [ 16 - 18 ]. (hindawi.com)
  • These genes are transcriptionally deregulated as the partner genes of IgH translocation, and are thus thought to perform crucial roles in human B-cell lymphomagenesis. (haematologica.org)
  • 2 A synergistic effect resulting from a combination of two genes from BCL2 , MYC and CCND1 has been implicated in human B-cell lymphomagenesis. (haematologica.org)
  • the development of T cells, we generated a mouse strain having a disruption in both CD3 and CD3 genes (CD3? (tech-strategy.org)
  • A lower number of foster clones was associated with a higher risk group after prephase and t (12;21)/ ETV6:RUNX1 genetic type. (frontiersin.org)
  • Poly(I:C) treatment of pancreatic adenocarcinomas followed by coculture with γδ T cells resulted in an upregulation of CD54 on the tumor cells. (aacrjournals.org)
  • The malignant clone secretes increased amounts of distinct collagenases compared to the non-tumorigenic cell line, thereby verifying a correlation between biological behaviour and the amount of collagenases. (uni-bielefeld.de)
  • the silencing of FOXC1 inhibited the biological function of AGS and MKN28 cells. (hindawi.com)
  • In this study, we investigated the inhibitory effects of miR-149 on the biological functions of gastric cancer cells by targeting FOXC1. (hindawi.com)
  • In contrast to mice deficient in CH5424802 biological activity either CD3 or CD3 chains, early thymic development mediated by pre-TCR is completely clogged, and TCR-+ or TCR-+ T cells were absent in the CD3? (tech-strategy.org)
  • These observations are consistent with the biological evidence that in mature T cells, the TCRC CD3 complex lacking either CD3 or could sometimes be detected on the cell surface at reduced levels. (tech-strategy.org)
  • Since T cells normally take into account only an extremely small percentage of thymocytes and peripheral T cells, we evaluated T cell advancement in the tiny intestine, where T cells represent a significant population from the iIEL in wild-type mice. (tech-strategy.org)
  • This importance is focussed on the target binding site - epitope, where epitope selection as a part of design thinking beyond traditional antigen selection using whole cell or whole protein immunisation can positively impact success. (preprints.org)
  • Furthermore, none of the 6 diatom species tested were toxic on normal human cells. (frontiersin.org)
  • The bovine leukemia virus (BLV) belongs to the family Retroviridae (genus Deltaretrovirus ) and is closely related to human T-cell leukemia viruses (HTLV-1 and II) [ 1 ]. (biomedcentral.com)
  • Together, our data indicate that SLAMF receptors are important modulators of the BCR signaling axis and may improve immune control in CLL by interference with NK cells. (nature.com)
  • Signaling Lymphocytic Activation Molecule Family (SLAMF) receptors are a group of nine type I transmembrane receptors that are mainly expressed on a variety of immune cells. (nature.com)
  • The construction of this cell line is the first promising step in the regulation of GAL secretion from hTERT-immortalized BMSCs, and the potential application of this system may provide a stem cell-based research platform for pain. (hindawi.com)
  • Therefore, these analyses indicate that deficiency in CD3 and blocked T cell advancement beyond the CD8+ stage completely. (tech-strategy.org)
  • In addition, they had ductal arrangements or plasmacytoid appearances of tumor cells and myxoid or hyaline stromata, indicating some characteristics of pleomorphic adenoma. (biomedcentral.com)
  • Cell lines from various tumor types have been developed for use as a tumor model for controlled laboratory studies. (biomedcentral.com)
  • Transcripts of MMP-1, -8, -13 and TIMP-1 were detected in all cell lines by RT-PCR and the corresponding proteins were found in the cytoplasm of all three cell lines by Western blot analysis and/or immunocytochemistry. (uni-bielefeld.de)
  • TIMP-1 was found only in extracts of all three cell lines, predominantly in A5. (uni-bielefeld.de)
  • In addition, we provide clear evidence that MMP-8 is not exclusively found in polymorphonuclear granulocytes, but also in keratinocyte cell lines. (uni-bielefeld.de)
  • Here, an hTERT-BMSCs/Tet-on/GAL cell line was constructed using a single Tet-on-inducible lentivirus system, and subsequent experiments demonstrated that the secretion of rat GAL from hTERT-BMSCs/Tet-on/GAL was switched on and off under the control of an inducer in a dose-dependent manner. (hindawi.com)
  • qRT-PCR was used to detect the expression of miRNA-149 and FOXC1 in gastric cancer tissues and cells. (hindawi.com)
  • These results indicate that clonally expanded Vd4 T cells may represent a reconstituted γδ T cell repertoire after HSCT, which also hints to a relatively better outcome for this case. (psu.edu)
  • In download to the additional essence of scholars, specialists, such evolution, Louisianans, and publications, there have sixteen long welfare searches on unfair posts helping adaptive American light, clone mathsNumber, and richness. (lightseed.com)
  • 3. Patients with a higher number of foster clones were patients in the better prognosis group. (frontiersin.org)
  • Culturing conditions (i.e., nutrient starvation conditions) greatly influenced bioactivity of the majority of the clones/species tested. (frontiersin.org)
  • In B cells, this class of receptors has been shown to be expressed in distinct patterns associated with development and activation [ 11 ]. (nature.com)
  • γδ T-cell cytotoxicity and granzyme A/B production were enhanced after pretreatment of tumor cells with TLR3 [poly(I:C)] or TLR7 ligand (imiquimod). (aacrjournals.org)
  • The interaction of CD54 and the corresponding ligand CD11a/CD18 expressed on γδ T cells is responsible for triggering effector function in γδ T cells. (aacrjournals.org)