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  • regulate
  • We demonstrate that both NoLS are functional and necessary for nucleolar localization of specifically myosin IC isoform B. Our data provide a first mechanistic explanation for the observed functional differences between the myosin IC isoforms and are an important step toward our understanding of the underlying mechanisms that regulate the various and distinct functions of myosin IC isoforms. (nih.gov)
  • interference
  • In eukaryotes, Argonautes (eAgos) play central roles in RNA interference (RNAi) and micro-RNA (miRNA) pathways that coordinate a wide range of cellular processes including transcriptional and translational gene regulation ( 2 , 3 ), silencing of mobile genetic elements ( 4 , 5 ), and host defense ( 6 ). (pnas.org)
  • Ingestion of genetically modified yeast symbiont reduces fitness of an insect pest via RNA interference. (ucdavis.edu)
  • Here, we used RNA interference to investigate the function of POLID in procyclic T. brucei . (asm.org)
  • Depletion of AIR-2 by RNA interference (RNAi) prevents chromosome separation at both anaphases, with concomitant prevention of meiotic cohesin REC-8 release from meiotic chromosomes. (stemcell8.cn)
  • bind
  • Like sqd , hrb27C mutants lay eggs with dorsoventral defects and Hrb27C can directly bind to grk RNA. (biologists.org)
  • eAgos bind single-stranded RNAs (ssRNA), such as small interfering RNAs (siRNAs), miRNAs, or PIWI (P-element induced wimpy testis)-interacting RNAs (piRNAs), which act as templates for the recognition of complementary ssRNA targets, leading either to Ago cleavage of the targeted RNA ( 7 ) or to the recruitment of additional components of the RNA degradation machinery ( 8 ⇓ - 10 ). (pnas.org)
  • FXR1P muscle-specific isoforms bind p21 mRNA via direct interaction with a conserved G-quadruplex located in its 3′ untranslated region. (prolekare.cz)
  • These residues were reported to bind RNA. (pnas.org)
  • The TEN domain interacts with the ss telomere DNA repeats, the TRBD domain binds multiple sites of TR, and the RT and C-terminal extension domains bind the RNA/DNA hybrid and catalyze the addition of DNA repeats onto the 3′ end ( 27 , 31 - 34 ). (pnas.org)
  • specificity
  • Here we show that using 5-bromo-2′-deoxyuridine (BrdU) as the 5′ nucleotide of gRNAs stabilizes in vitro reconstituted CRISPR-associated Marinitoga piezophila Argonaute-gRNA complexes (MpAgo RNPs) and significantly improves their specificity and affinity for RNA targets. (pnas.org)
  • Using these specificity features, we employed MpAgo RNPs to detect specific adenosine-to-inosine-edited RNAs in a complex mixture. (pnas.org)
  • Altogether, these data indicate that the HBR is unique among basic phosphoinositide-binding domains, because it integrates three regulatory components, PI(4,5)P 2 , myristate, and RNA, to ensure plasma membrane specificity for particle assembly. (pnas.org)
  • transcription
  • Spatiotemporal behavior of nuclear cyclophilin B indicates a role in RNA transcription. (embl-heidelberg.de)
  • Together, this study identifies ZMYND11 as an H3.3-specific reader of H3K36me3 that links the histone-variant-mediated transcription elongation control to tumour suppression. (proteopedia.org)
  • The small, 195-amino-acid form of the hepatitis delta virus (HDV) antigen (δAg-S) is essential for genome replication, i.e., for the transcription, processing, and accumulation of HDV RNAs. (asm.org)
  • These high-resolution structures have significantly advanced our understanding of how TERT catalyzes the reverse transcription of telomere DNA and how TERT could potentially interact with the ss telomere DNA and the template RNA/DNA hybrids. (pnas.org)
  • regulates
  • Through binding to its adenylate-cyclase-coupled receptor MC2R, ACTH stimulates the release of cAMP and the activation of the cAMP-dependent protein kinase A (PKA), which in turn phosphorylates and regulates a number of specific substrates including the steroidogenic acute regulatory protein (StAR) ( 1 ) and the cAMP response element-binding protein (CREB) ( 2 ). (frontiersin.org)
  • Importantly, we found that RNA also negatively regulates Gag membrane binding. (pnas.org)
  • isoforms
  • two RNA-binding domains, auxiliary glycine-rich and M9-like domains, and existence in multiple protein isoforms. (biologists.org)
  • Three different HOW isoforms are generated that all share a KH RNA-binding domain and differ in their C-terminal tails. (biologists.org)
  • In mammalian cells, three isoforms of myosin IC are expressed that differ only in the addition of short isoform-specific N-terminal peptides. (nih.gov)
  • In this study, we used EGFP-fusion constructs that express truncated or mutated versions of myosin IC isoforms to detect regions that are involved in isoform-specific localization. (nih.gov)
  • We use mass spectrometry-based approaches which can do more than simply determining the identity of a protein isolated in a polyacrylamide gel -- rapidly advancing technologies are allowing us to measure dynamic changes in protein abundances, post-translational modification states, splice isoforms, interaction partners, and localization across multiple cell states. (stanford.edu)
  • affinity
  • Electrophoretic gel mobility shift experiments showed that the Arabidopsis AtPum2 PUM-HD binds with high affinity to BoxB of the Drosophila Nanos Response Element I (NRE1) RNA, whereas a point mutation in the core of the NRE1 resulted in a significant reduction in binding affinity. (biomedcentral.com)
  • By modifying the 5′-nucleotide of the gRNA, we demonstrate that MpAgo-gRNA complexes (RNPs) are easily programmable, have high affinity to fully complementary RNA substrates, and can discriminate by more than 300-fold between substrates that differ by only a single nucleotide. (pnas.org)
  • Several analyses, based on phylogenetic trees for 16S rRNA and the presence/absence of an 11-aa insertion in EF-1α, placed M. kandleri close to the root of the Euryarchaeota and did not suggest any specific affinity with other archaeal methanogens ( 13 - 15 ). (pnas.org)
  • situ
  • In situ RNA hybridization localized PvUPS1 to the nodule endodermis and the endodermis and phloem of the nodule vasculature. (plantphysiol.org)
  • Bio-Techne announced the launch of its RNAscope VS Duplex kit, which delivers simultaneous in situ detection of two RNA species on an automated platform. (genomeweb.com)
  • pathways
  • Although I deal primarily with nuclear RNA export in metazoan cells, it is clear that these export pathways are highly conserved among eukaryotes and I will therefore also rely extensively on data obtained in the yeast Saccharomyces cerevisiae , which has proven to be a powerful genetic system to identify critical components of several nuclear RNA export pathways. (biologists.org)
  • binds
  • RNA binds to the inner concave surface, with the N terminus of the protein bound to the 3′ end of the RNA ( 27 ). (pnas.org)
  • circular
  • HDV particles contain a ribonucleoprotein (RNP) core composed of the circular 1.7-kb RNA genome and multiple copies of the delta antigen (δAg), the only HDV-encoded protein ( 25 ). (asm.org)
  • regulatory
  • They reveal unexpected plasticity in the PUF-RNA interactions achieved via alternative base flipping patterns and add a previously undescribed layer of biological meaning in the signals that govern mRNA regulatory control. (pnas.org)
  • cytoplasmic
  • We also observe a direct physical interaction between Hrb27C and Ovarian tumor (Otu), a cytoplasmic protein implicated in RNA localization. (biologists.org)
  • In most cell types, Gag assembles on the cytoplasmic leaflet of the plasma membrane (PM). MA is required for this PM-specific targeting as well as the lipid bilayer binding of Gag. (pnas.org)
  • intracellular
  • Unexpectedly, two other HBR residues, Lys25 and Lys26, suppress membrane binding in the absence of PI(4,5)P 2 and prevent promiscuous intracellular localization of Gag. (pnas.org)
  • interactions
  • These findings broaden our mechanistic understanding of the interactions of Argonautes with guide and substrate RNAs, and demonstrate that MpAgo RNPs with 5′-BrdU-modified gRNAs can be used as a highly specific RNA-targeting platform to probe RNA biology. (pnas.org)
  • structural
  • Structural studies show that in addition to the trimethyl-lysine binding by an aromatic cage within the PWWP domain, the H3.3-dependent recognition is mediated by the encapsulation of the H3.3-specific 'Ser 31' residue in a composite pocket formed by the tandem bromo-PWWP domains of ZMYND11. (proteopedia.org)
  • human diseases
  • Failure of such mechanisms, in particular misexpression of RNA-BP, has been linked to several human diseases. (frontiersin.org)
  • Telomerase deficiency because of mutations in human telomerase RNA (hTR) has also been linked to several inherited human diseases, such as dyskeratosis congenita, aplastic anemia, myelodysplasia, and idiopathic pulmonary fibrosis ( 13 - 26 ). (pnas.org)
  • functional
  • This will be useful for the researchers in their proper usage thereby guiding them toward novel hypotheses generation and saving time and costs involved in extensive experimental processes in these three different novel functional RNAs. (deepdyve.com)
  • The protein TERT is highly conserved across different species, and it usually contains four major functional domains: the TERT N-terminal domain (TEN), the TERT RNA binding domain (TRBD), the reverse transcriptase domain (RT), and the C-terminal extension ( 27 , 30 - 32 ). (pnas.org)
  • interaction
  • We sought to probe several PUF/RNA complexes to identify those features of the interaction that were general, as well as those that were idiosyncratic. (pnas.org)
  • We and others have shown that Gag interacts with a plasma-membrane-specific acidic phospholipid, phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P 2 ], via the HBR, and that this interaction is important for efficient membrane binding and plasma membrane targeting of Gag. (pnas.org)
  • In addition, MA, especially the HBR, has been shown to interact with RNA ( 15 ⇓ ⇓ ⇓ - 19 ) although the role of this interaction in virus assembly is less well understood. (pnas.org)
  • biology
  • New discoveries and accelerating progresses in the field of noncoding RNAs (ncRNAs) continuously challenges our deep-rooted doctrines in biology and sometimes our imagination. (mdpi.com)
  • repeats
  • Telomerase is a unique reverse transcriptase that catalyzes the addition of telomere DNA repeats onto the 3′ ends of linear chromosomes and plays a critical role in maintaining genome stability. (pnas.org)
  • The 3′ ends of telomeres are replicated by telomerase, a unique reverse transcriptase discovered almost three decades ago ( 7 ), which catalyzes the addition of telomere DNA repeats onto the ends of linear chromosomes using an embedded RNA as the template ( 8 , 9 ). (pnas.org)
  • degradation
  • 2001). In Saccharomyces cerevisiae, activation of separase at anaphase I and II results in the degradation of the meiotic-specific cohesin molecule, Rec8p (Buonomo et al. (stemcell8.cn)
  • presumably
  • under these conditions, the RNA presumably enters the cells already complexed with a mixture of δAg-S and δAg-L ( 1 ). (asm.org)
  • structures
  • In addition to allowing super-resolution imaging, SPLM provides inherent molecular-specific spectroscopic information of targeted structures visualized when dyes are used. (spiedigitallibrary.org)
  • To better understand this requirement, we used purified recombinant δAg-S and HDV RNA synthesized in vitro to assemble high-molecular-weight ribonucleoprotein (RNP) structures. (asm.org)
  • cleavage
  • It stabilizes HDV RNA circles ( 18 ), and it facilitates ribozyme cleavage ( 13 ). (asm.org)