• Effects of mitochondrial uncouplers on intracellular calcium, pH and membrane potential in rat carotid body type I cells. (ox.ac.uk)
  • In addition to confocal microscopy and flow cytometry, ASCs were analyzed for metabolic (OXPHOS, glycolysis), morphological (cytoskeleton), and mitochondrial (ATP production, membrane potential) changes. (bvsalud.org)
  • The time effects of acidic pH e stress were determined with respect to tumor cell proliferation, phenotypic regulation, autophagic control, metabolic plasticity, mitochondrial network dynamics, and metastatic potentials. (biomedcentral.com)
  • Both 2,4-dinitrophenol (DNP) and carbonyl cyanide p-trifluoromethoxyphenyl hydrazone (FCCP) caused an increase in [Ca2+]i which was largely inhibited by removal of extracellular Ca2+ or Na+, or by the addition of 2 mM Ni2+. (ox.ac.uk)
  • In this study we investigated the effects of over-expressing or silencing of sFRP4 in ovarian cancer lines with different chemosensitivity in an in vitro model. (biomedcentral.com)
  • Furthermore, in vitro culturing conditions can have a large effect on cell growth (11-13) and thus, cellular metabolism. (researchsquare.com)
  • A few studies have documented biological effects of urolithins in vitro, including antiproliferation in cancer cell models10, anti-inflammation11 and benefits on lipid metabolism12, although a clear biological pathway has not yet been described. (lsd1receptor.com)
  • In addition, ASCs exposed to M1-inflammatory stress demonstrated reduced migration, switched to a glycolysis-dominated metabolism with reduced ATP production, and increased levels of inflammatory cytokines such as IL1B, IL8, and MCP1. (bvsalud.org)
  • To test the first possibility, we measured the effect of UA on bacterial metabolism by measuring the growth of two Escherichia coli strains, OP50 and HT115, after treatment with UA. (lsd1receptor.com)
  • 4. FCCP and DNP also depolarized type I cell mitochondria. (ox.ac.uk)
  • These effects are unlikely to be caused by cell acidification. (ox.ac.uk)
  • We tested four major extracts of M. charantia and found that momordicine I reduced glioma cell viability without serious cytotoxic effects on astrocytes. (excli.de)
  • We further explored whether the antiglioma effect of momordicine I may be related to cell cycle modulation and DLGPA5 expression. (excli.de)
  • In addition to identifying VHL as a critical regulator of nephron progenitors' metabolic switching, the authors' findings demonstrate that this switch also plays a large role in the differentiation process, and suggest that VHL is required for normal kidney development. (lww.com)
  • Bitter melon is attracting increasing attention because of its beneficial effects against obesity and type 2 diabetes-associated metabolic disorder (Fan et al. (excli.de)
  • This shift in metabolic pathways is called "The Warburg Effect" and results in the accumulation of lactate regardless of oxygen availability (4). (researchsquare.com)
  • This can confound metabolic analyses compared with the primary tumour due to the effects from the tumour microenvironment (10). (researchsquare.com)
  • The biological effects of urolithins remain poorly characterized, despite wide-spread human exposure via the dietary consumption of their metabolic precursors, the ellagitannins, which are found in the pomegranate fruit, as well as in nuts and berries. (lsd1receptor.com)
  • We have therefore investigated their effects upon isolated type I cells. (ox.ac.uk)
  • Our results indicate that the cytotoxic effect of momordicine I on glioma cells suggests its potential therapeutic application to GBM treatment. (excli.de)
  • Using the mitochondrial uncoupler carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) and thapsigargin to empty intracellular Ca 2+ stores, we found that the initial increase originates from emptying of the endoplasmic reticulum and consequent influx of extracellular Ca 2+ , whereas the late increase originates primarily from mitochondria. (nih.gov)
  • Because MTG was recently reported to be sensitive to MMP, we have reevaluated the effects of loss of MMP on MTG and CMXRos fluorescence, using both flow cytometry and laser scanning confocal microscopy (LSCM). (nih.gov)
  • METHODS: Using flow cytometry, the relative fluorescence of CMXRos, R123, and MTG was determined in human lymphoblastoid cell lines (LCLs) with or without carbonyl cyanide p-trifluoromethoxylphenyl-hydrazone (FCCP), used to collapse the MMP. (nih.gov)
  • LSCM analysis was also used to evaluate the effect of FCCP on MTG and CMXRos fluorescence of mouse cells and viable lenses in culture. (nih.gov)
  • The metabolic phenomenon describing increased glycolytic capacity in cancer cells, known as "the Warburg effect" [ 6 ] , stimulated decades of research directed towards the characterization of the reprogramming of energy metabolism during cellular transformation and its role in tumor development. (oaepublish.com)
  • Warburg effect or aerobic glycolysis is a common feature of cancer cells. (oncotarget.com)
  • To further evaluate the effects of the overexpression of the Bcl-2 homologue Buffy , this study analysed lifespan and climbing ability in both parkin -RNAi- and Pink1 -RNAi-expressing flies. (sdbonline.org)
  • Increasing concentrations of brominated flame retardants, in particular polybrominated diphenyl ethers (PBDEs), in the environment, human food chain, and human tissues raise concern about possible neurotoxic effects. (nih.gov)
  • These effects were inhibited by addition of a cyclooxygenase 2 inhibitor (NS398) or an E-prostanoid receptor 4 antagonist (AH23848) to MMDD1 or CD44+ cells, respectively. (bvsalud.org)
  • In addition, current prognostic markers are unable to accurately predict the risk of metastasis development and approximately 30% of patients first diagnosed with earlier-stage breast cancer will eventually develop recurrent metastatic disease [ 3 ] . (oaepublish.com)
  • In addition, enhanced miR-448 level was significantly associated with poor clinical outcomes of GC patients. (oncotarget.com)