• We have cloned and sequenced the structural gene for this ACP and find that it does encode a discrete small protein. (asm.org)
  • In this system the ACP was efficiently expressed at levels 10 to 20% of total cell protein. (asm.org)
  • Fatty acid synthesis in S. erythraea has previously been proposed to involve a discrete acyl carrier protein (ACP), as in most prokaryotic fatty acid synthases. (asm.org)
  • In the biosynthesis of fatty acids, the β‐ketoacyl‐acyl carrier protein (ACP) synthases catalyze chain elongation by the addition of two‐carbon units derived from malonyl‐ACP to an acyl group bound to either ACP or CoA. (embopress.org)
  • β-ketoacyl-acyl carrier protein (ACP) synthases (KAS) from various bacterial species have been cloned, expressed and purified in large quantities for detailed enzymological, structural and screening studies. (eurekaselect.com)
  • Sanjay S. Khandekar, Robert A. Daines and John T. Lonsdale, " Bacterial β-ketoacyl-Acyl Carrier Protein Synthases as Targets for Antibacterial Agents", Current Protein & Peptide Science (2003) 4: 21. (eurekaselect.com)
  • Christensen QH, Grove TL, Booker SJ, Greenberg EP (2013) A high-throughput screen for quorum-sensing inhibitors that target acyl-homoserine lactone synthases. (springer.com)
  • Many fungal PKSs have an architecture based on single modular iterative type I polyketide synthases (iPKSs), containing ketosynthase (KS), acyltransferase (AT) and acyl carrier protein (ACP) domains. (biomedcentral.com)
  • Our results also suggest that the MAT does not discriminate against α-substituted acyl-CoA thioesters solely on the basis of substrate size. (elsevier.com)
  • Using acetyl-CoA and malonyl-CoA as the initiating substrate and building block, respectively, these organisms share a common set of biochemical reactions to extend fatty acyl chains by two carbon atoms per cycle [ 1 - 3 ]. (beds.ac.uk)
  • We have cloned and sequenced the structural gene for this ACP and find that it does encode a discrete small protein. (asm.org)
  • The peroxisome proliferator (PP) response element (PPRE) upstream of the human acyl CoA oxidase gene is inactive in a sample human population: significance for species differences in response to PPs. (thefreedictionary.com)
  • cite the finding of an inactive PP response element upstream of the acyl CoA oxidase gene in a sample human population (15) as additional evidence for why humans may be less responsive than rodents to PPs. (thefreedictionary.com)
  • Here, we report on the development of a clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated protein 9 (CRISPR-Cas9)-mediated strategy for creating gene disruption mutants and the application of this technique for exploring roles of known and hypothesized virulence factors. (asm.org)
  • Gene annotation of the identified proteins was corrected by experimental data, and an interesting case of alternative splicing was discovered. (plantcell.org)
  • Importantly, the sequenced genome also makes it possible to take advantage of the dramatic improvements in biological mass spectrometry to study gene expression directly at the protein level and to determine protein localization and post-translational modifications efficiently in a systematic manner. (plantcell.org)
  • The sequence of ENR is highly conserved within higher plants and a homology model of Arabidopsis ENR was derived from the crystal structure of the protein from Brassica napus . (plantphysiol.org)
  • RNA and protein-blot analysis in Arabidopsis and rapeseed ( Brassica napus ) showed that the endogenous AtFATB1 expression was highest in flowers and lower in leaves. (plantphysiol.org)
  • Small volumes of fabZ recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. (mybiosource.com)
  • In order to investigate whether the relative activities of the plastidial acyl-acyl carrier protein (ACP):glycerol 3-phosphate acyltransferase (EC 2.3.1.15) and acyl-ACP hydrolase play a role in controlling the acyl flux through the prokaryotic and eukaryotic pathway, we determined these enzymic activities in stroma fractions from 16:3- and 18:3-plants using glycerol 3-phosphate and labelled acyl-ACP as substrates. (springer.com)
  • Consequently the relative activities of the plastidial glycerol 3-phosphate acyltransferase and acyl-ACP hydrolase can play a decisive role in controlling the acyl flux through the different pathways at least in these plants. (springer.com)
  • Douady, D., Dubacq, J.-P. (1987) Purification of acyl-CoA: glycerol 3-phosphate acyltransferase from pea leaves. (springer.com)
  • The structural studies revealed that attachment of butyryl acyl intermediate to ACP alters the conformation of ACP. (rcsb.org)
  • All of the proteins required to catalyze one cycle of fatty acid synthesis from acetyl-CoA plus malonyl-CoA to butyryl-ACP in vitro were purified. (semanticscholar.org)
  • The structures of a number of acyl carrier proteins have been solved using various NMR and crystallography techniques. (wikipedia.org)
  • To investigate the role of the minor states of an acyl carrier protein, NMR techniques were applied to determine the number of minor states and characterize their structures and kinetics. (nus.edu.sg)
  • The ability to rapidly obtain structures of protein-ligand complexes using X-ray crystallography is central to drug discovery, but the typical cryocooling of samples and the effects of the X-ray beam may distort the observed ligand binding. (iucr.org)
  • X-ray free-electron lasers (XFELs) have the promise to solve these issues, but methods to rapidly produce structures of protein-ligand complexes at XFELs have not yet been realized. (iucr.org)
  • Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). (uniprot.org)
  • Most Streptomyces chromosomal DNA molecules are about 8-Mb-long, with terminal-inverted repeats and covalently bound terminal proteins supposedly at the 5′ end. (pnas.org)
  • The work outlined in this thesis aims to answer questions that remain about how acyl carrier proteins interact with bound acyl groups and with catalytic domains in these important metabolic pathways. (escholarship.org)
  • Each module contains a set of three domains-a ketosynthase, an acyltransferase (AT), and an acyl carrier protein (ACP)-that catalyze a 2-carbon extension of the growing polyketide chain (Fig. 1 ) ( 9 ). (pnas.org)
  • Experimental proteome analysis was combined with a genome-wide prediction screen to characterize the protein content of the thylakoid lumen of Arabidopsis chloroplasts. (plantcell.org)
  • If you cannot find the target and/or product is not available in our catalog, please click here to contact us and request the product or submit your request for custom elisa kit production , custom recombinant protein production or custom antibody production . (mybiosource.com)
  • View conserved domains detected in this protein sequence using CD-search. (nih.gov)
  • See the reference protein sequence for acyl carrier protein (NP_627461.1). (nih.gov)
  • Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (uniprot.org)
  • The availability of the Arabidopsis genome now allows the classification of proteins according to predicted domain(s) and subcellular localization. (plantcell.org)
  • Luca Picone-Chiodo, who was at high risk of having MCAD (Medium-chain acyl CoA dehydrogenase) a genetic condition that affects the metabolism, died at his family home in Huddersfield on October 16, 2009. (thefreedictionary.com)
  • Diagnosis of medium chain acyl CoA dehydrogenase deficiency by measurement of cis-4-decenoic acid in dried blood spots. (thefreedictionary.com)