Activity vitro and cell. Medical search. Frequent questions

Methodologically, the study showed that silicone can be used as reference partitioning phase to transfer in vivo exposure in humans (silicone implants) to in vitro assays (partition-controlled dosing). (nih.gov)
Cell attachment, proliferation and differentiation were studied using enzyme-linked immunosorbant assays, and immunofluorescence microscopy was used to detect integrin subunits and cytoskeletal arrangement 24 h after cellular attachment. (ucl.ac.uk)
Biochemical assays used to measure cellular attachment and differentiation showed that there were no significant differences in the activity of cells grown on the different substrates. (ucl.ac.uk)
The focus-forming cells exhibited stem properties as indicated by stem cell markers expression, sphere formation, and ALDH activity assays. (cdc.gov)
In vivo animal studies supported the in vitro findings and indicated the potential utility of FSC-based assays as a rapid screening tool for fibrogenicity testing of nanomaterial s. (cdc.gov)
We determined their in vitro antioxidant activity using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), nitric oxide (NO) and superoxide anion (SO) assays, and their phytochemical profile by spectrophotometric and LC-MS/MS techniques. (unicatt.it)
I-X action on two acute myeloid leukemia (AML) cell lines viability, apoptosis and cell cycle were evaluated by MTS, western blotting and cytofluorimetric assays. (unicatt.it)
For this purpose, we performed in vitro assays, proving that Lf exerts an antiviral activity against SARS-COV-2 through direct attachment to both SARS-CoV-2 and cell surface components. (frontiersin.org)
In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc. (cdc.gov)
We have generated a high affinity and selective humanized monoclonal IgG4 antibody, TSR-033, which binds human LAG-3 and serves as a functional antagonist, enhancing in vitro T-cell activation both in mixed lymphocyte reactions and staphylococcal enterotoxin B-driven stimulation assays. (aacrjournals.org)

Compared to the 3D binding model where the reactants are described in terms of concentration, the 2D model predicts less sensitivity of trimer formation to varying cell densities, which better matches changes in EC 50 from in vitro cytotoxicity assay data with varying E:T ratios. (springer.com)
In addition, when translating in vitro cytotoxicity data to predict in vivo active clinical dose for blinatumomab, the choice of model leads to a notable difference in dose prediction. (springer.com)
Antiproliferative activity and cytotoxicity of Solanum jamesii tuber extracts on human colon and prostate cancer cells in vitro. (psu.edu)
The objective of this study was to investigate antiproliferative activity and cytotoxicity of tuber extracts from 15 Solanum jamesii accessions on human HT-29 colon and LNCaP prostate cancer cell lines in vitro. (psu.edu)
Dive into the research topics of 'Antiproliferative activity and cytotoxicity of Solanum jamesii tuber extracts on human colon and prostate cancer cells in vitro. (psu.edu)
Even 10,000 µg/ml SPS had no obvious cytotoxicity toward HCE-T and Vero cells. (molvis.org)
Cytotoxicity, antiproliferative and apoptosis-inducing capacity of ACNVs against hepatoma carcinoma cell were assessed. (dovepress.com)
Despite having a limited effect on L929 normal cells, OEO/thymol induced cytotoxicity in MDA-MB231 breast cancer monolayers (2D) and to a lesser extent in MDA-MB231 spheroids (3D). (irost.org)
The purpose of this study was to screen an array of antiviral compounds for anti-FIPV (serotype II) activity, determine cytotoxicity safety profiles of identified compounds with anti-FIPV activity, and strategically combine identified monotherapies to assess compound synergy against FIPV in vitro. (vin.com)
Cytotoxicity was assessed using the MTT assay (cell viability) and Griess reagent (NO release). (bvsalud.org)
The response pattern varied depending on time and type of cell line used for analysis, although the results indicate a higher cytotoxicity for EPH in short-term tests. (bvsalud.org)
In spite of the advantages of the in vitro tests, they are not able to mimic the orchestrated role of cells present inperiradicular region and the long-term cytotoxicity presented by the sealers. (bvsalud.org)
Although the cytotoxicity of endodontic sealers has been extensively investigated, most previous studies have used fibroblast cell lines. (bvsalud.org)
In order to take a new approach to previous in vitro studies on the cytotoxicity of endodontic materials, it would be interesting to evaluate the response of fibroblasts and macrophages, which are cell lines involved in periapical inflammation and repair. (bvsalud.org)
The generated series and parental molecule were tested in vitro for antiparasitic activity against Trypanosoma cruzi, Leishmania braziliensis, and L. amazonensis, and cytotoxicity using RAW cells. (lu.se)

Vehicle-treated control cells did not show any hallmarks of apoptosis after 18 h of exposure to dimethyl sulphoxide. (up.ac.za)
Only 0.5% of 2 ME-treated cells showed characteristics of apoptosis. (up.ac.za)
Conversely, increased morphological hallmarks of apoptosis were observed in SNO-treated cells after 21.5 h of 2 ME exposure. (up.ac.za)
When compared to the 0.5% in vehicle-treated cells, 4.7% of cells were in apoptosis. (up.ac.za)
The present preliminary study suggests that the accumulation observed in metaphase cells and the increase in Cdc2 kinase activity caused by 2 ME are consistent with morphological hallmarks of mitotic arrest and disrupted mitotic spindle formation, thus leading to induction of apoptosis in SNO cells. (up.ac.za)
ACNVs were found to possess specific antitumor cell proliferation activity associated with an apoptosis-inducing pathway. (dovepress.com)
They activated intrinsic apoptotic pathway, induced a G0/G1 block in leukemic cells and, interestingly, led to apoptosis in patient AML cells. (unicatt.it)
As the scientists increased the CQA concentration, cancer cells died as a result of apoptosis. (sciencedaily.com)
The ability to induce apoptosis, or cell death, is a promising tactic for therapeutic interventions in all types of cancer, she said. (sciencedaily.com)
Apoptosis was determined by cell death assay and western blotting. (biomedcentral.com)
Triphala-mediated reduced cell survival correlated with induction of apoptosis, which was associated with reactive oxygen species (ROS) generation. (biomedcentral.com)
Triphala-induced apoptosis was linked with phosphorylation of p53 at Ser-15 and ERK at Thr-202/Tyr-204 in Capan-2 cells. (biomedcentral.com)
Pretreatment of cells with pifithrin-α or U0126, specific inhibitors of p53 or MEK-1/2, significantly attenuated Triphala-induced apoptosis. (biomedcentral.com)
Similarly, Triphala induced apoptosis in another pancreatic cancer cell line BxPC-3 by activating ERK. (biomedcentral.com)
On the other hand, Triphala failed to induce apoptosis or activate ERK or p53 in normal human pancreatic ductal epithelial (HPDE-6) cells. (biomedcentral.com)
Reduced tumor-growth in Triphala fed mice was due to increased apoptosis in the tumors cells, which was associated with increased activation of p53 and ERK. (biomedcentral.com)

Conditioned media from such cells were then used to stimulate human aortic endothelial cells (HAoECs) to enhance tube-like structure formation in a Matrigel assay. (hindawi.com)
Then, we used atorvastatin-stimulated cells or conditioned media from them in the Matrigel plug in vivo angiogenic assay. (hindawi.com)
This study establishes a new link between actual human internal POP levels and the endocrine active dose in vitro, applying partitioning-controlled dosing from silicone to the H295R steroidogenesis assay: (1) Measured concentrations of POPs in silicone breast implants were taken from a recent study and silicone disks were loaded according to these measurements. (nih.gov)
All extracts were testedfor cytotoxic activity by sulforhodamine B (SRB) assay. (bvsalud.org)
the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to evaluate the metabolic cell activity. (unifi.it)
In Vitro Toxicity Studies: Cell viability (dye exclusion): trypan blue assay etc. (cdc.gov)
In Vitro Toxicity Studies: Cell metabolic activity: Alamar Blue assay etc. (cdc.gov)
Flow cytometry, caspase-3 activity assay in treated monolayers/spheroids and also fluorescence staining and DNA fragmentation in treated monolayers demonstrated apoptotic death mode. (irost.org)
Growth-inhibitory effects of Triphala were evaluated in Capan-2, BxPC-3 and HPDE-6 cells by Sulphoradamine-B assay. (biomedcentral.com)
Gibberellic acid acts as an antagonist to abscisic acid activity in this assay. (usda.gov)

Moreover, JNK1 KO and WT D-JNKI-1 treated OLs, while not showing overt alterations of differentiation in vitro, display a reduced surface compared to controls. (nature.com)

Here, in the presence of KL+FL+megakaryocyte growth and development factor (MGDF), which recruits virtually all Lin(-)Sca-1(+)kit(+) bone marrow cells into proliferation and promotes their self-renewal under serum-free conditions, IL-3 and IL-11 revealed an indistinguishable ability to further enhance proliferation. (lu.se)
Extracellular ST6GAL1, present in cancer exosomes or the freely soluble recombinant sialyltransferase, compensates for insufficient intrinsic ST6GAL1 by boosting cancer cell proliferation and increasing invasiveness. (nature.com)
Our data indicate that extracellular ST6GAL1 from remote sources can compensate for cellular ST6GAL1-mediated aggressive tumor cell proliferation and invasive behavior and has great clinical potential for extracellular ST6GAL1 as these molecules are in the extracellular space should be easily accessible targets. (nature.com)
OPC architecture, proliferation and oligodendro-/myelino-genesis are finely regulated by the interplay of cell-intrinsic and extrinsic factors. (nature.com)
Our results unveil a novel player in the complex regulation of OPC biology, on the one hand showing that JNK1 ablation cell-autonomously determines alterations of OPC proliferation and branching architecture and, on the other hand, suggesting that JNK1 signaling in OLs participates in myelination in vivo. (nature.com)
Both a cell-intrinsic program and environmental factors participate in the regulation of OPC architecture, proliferation and oligodendro-/myelino-genesis. (nature.com)
ADSC conditioned media (CMADSC) were assessed for paracrine activity on keratinocyte proliferation and migration, with hypoxic conditions for ADSC culture used to generate contrasting effects on cytokine gene expression. (oslomet.no)
The tuber extracts significantly inhibited proliferation of HT-29 and LNCaP cell lines and were not cytotoxic to the cells compared to the control (DMSO). (psu.edu)
In both HT-29 and LNCaP cells, there were no observable significant correlations between antioxidant activity (DPPH and ABTS) and inhibition of cell proliferation or between TP and cell proliferation inhibition. (psu.edu)
Also, glycoalkaloids did not exhibit significant correlations with the inhibition of cancer cell proliferation. (psu.edu)
Correlation results, along with cell proliferation data, suggest that not only the compounds measured in this study but also other bioactive compounds present in the matrix acting additively or synergistically may be more responsible for the antiproliferative effects of potato tuber extracts than higher concentrations of a single or group of compounds. (psu.edu)
In the present study, we demonstrate that Triphala significantly inhibit the proliferation of Capan-2 and BxPC-3 human pancreatic cancer cells. (biomedcentral.com)
Analogous studies in a murine syngeneic tumor model using surrogate antibodies demonstrated significant synergy between LAG-3 and PD-1 blockade-combination treatment led to a marked improvement in therapeutic efficacy, increased T-cell proliferation, IFNγ production, and elicited durable immunologic memory upon tumor rechallenge. (aacrjournals.org)

Taken together, our study suggests that inhibition of HDAC by sorafenib may provide clinical benefits against HCC, and enhancement of HDAC-inhibitory activity of sorafenib may improve its therapeutic efficacy. (oncotarget.com)
The aim of this study was to analyze the in vitro antimicrobial efficacy and the in vitro effects of an ophthalmic formulation containing hexamidine diisethionate 0.05%, polyhexamethylene biguanide (PHMB) 0.0001% disodium edetate (EDTA) 0.01%, dexpanthenol 5% and polyvinyl alcohol 1.25% (Keratosept, Bruschettini, Genova, Italy) on cultured human corneal and conjunctival cells. (unifi.it)
Imatinib has shown preclinical activity against HRPC although minimal clinical therapeutic efficacy. (ingentaconnect.com)
In a humanized mouse non-small cell lung carcinoma model, TSR-033 boosted the antitumor efficacy of PD-1 monotherapy, with a concomitant increase in immune activation. (aacrjournals.org)

Such progress provides us now with new opportunities to consider alternative approaches to sensitization testing, including the design of in vitro test methods. (cdc.gov)
Described here are some of the general requirements of in vitro test methods for skin sensitization, and progress that has been made in developing suitable approaches with particular emphasis on the utility of dendritic cell culture systems. (cdc.gov)
1999), there has been little investment or progress in based solely on in vitro methods. (cdc.gov)

SPS inhibits HSV-1 replication in HCE-T and Vero cells, indicating that SPS has the potential for treating HSV-1 infection, particularly HSV-1 keratitis. (molvis.org)
moreover, it inhibits the growth of HL60 cells with a parallel stimulus of differentiation. (unicam.it)

We found that shRNA knockdown of intrinsic ST6GAL1 expression resulted in decreased ST6GAL1 cargo in the exosome-like vesicles as well as decreased breast tumor cell growth and invasive behavior in 3D in vitro cultures. (nature.com)
Keratinocytes cultured in CMADSC showed a significant increase in cell number compared to serum-free cultures and further significant increases in hypoxic CMADSC. (oslomet.no)
In-vitro evaluation of apoptotic effect of OEO and thymol in 2D and 3D cell cultures and the study of their interaction mode with DNA. (irost.org)
Transcript abundance of the genes causing the increase in cell wall thickness was measured in cultures established under different plant hormone compositions. (usda.gov)

3) Hormone production of the adrenal cells was measured as toxicity endpoint. (nih.gov)
In vivo antitumor activity and toxicity were evaluated in Hep G2 cell xenograft model. (dovepress.com)
In Vitro Toxicity Studies: Cell membrane integrity: Cytoplasmic enzymes leakage (lactate dehydrogenase, ATP enzymes etc. (cdc.gov)

Most of I-X induced a dose/time dependent reduction of cell viability higher than parent extract. (unicatt.it)
IV and VI sub-fractions showed highest cytotoxic activity and, of note, a negligible reduction of healthy cell viability. (unicatt.it)

Arvedson T, Bailis JM, Britten CD, Klinger M, Nagorsen D, Coxon A et al (2022) Targeting solid tumors with bispecific T cell engager immune therapy. (springer.com)
Yoneyama T, Kim M-S, Piatkov K, Wang H, Zhu AZX (2022) Leveraging a physiologically-based quantitative translational modeling platform for designing B cell maturation antigen-targeting bispecific T cell engagers for treatment of multiple myeloma. (springer.com)

Interestingly, the in silico results strongly supported the hypothesis of a direct recognition between Lf and the spike S glycoprotein, which can thus hinder viral entry into the cells. (frontiersin.org)

Conversely, the PIEZO1 antagonist GsMTx4 reduces glucose-induced Ca 2+ -signaling, electrical activity and insulin secretion. (lu.se)

Cotton ovules, excised from ovaries prior to fertilization, will continue development in vitro under defined conditions when auxin and gibberellic acid are present. (usda.gov)

Development, Characterization and In Vitro Evaluation of Single o. (ingentaconnect.com)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Taken together, PBM is shown capable of restoring the cellular morphology and function of hypertrophic cells. (google.com)
Our preclinical studies demonstrate that Triphala is effective in inhibiting the growth of human pancreatic cancer cells in both cellular and in vivo model. (biomedcentral.com)

The in vivo antitumor activity of ART was similar to that of gemcitabine. (medicdebate.org)
Taken together, our study suggests that ART exhibits antitumor activity against human pancreatic cancer via a novel form of oncosis-like cell death, and that ART should be considered a potential therapeutic candidate for treating pancreatic cancer. (medicdebate.org)

Aggressive breast cancer is a highly heterogeneous disease caused by a variety of distinct cell-intrinsic genetic alterations in mammary epithelial cells, leading to vastly heterogenic disease manifestation in individual patients and predominantly affecting patient prognosis and treatment options [ 14 ]. (nature.com)
Human corneal and conjunctival epithelial cells in vitro were incubated for 5, 10 and 15 min with Keratosept or its components. (unifi.it)
the ophthalmic solution and its components were safe and non-toxic for the corneal and conjunctival epithelial cells for 5 and 10 min at the concentrations analyzed, and dexpanthenol 5% and polyvinyl alcohol promoted the wounding of corneal cells. (unifi.it)
In HHV-1 and HHV-2 oral infections, viral replication within the oral epithelium may cause lysis of epithelial cells, with vesicle formation. (medscape.com)

In vitro cytotoxic activity of Sa-Tri-Lhung-Klod remedy and its herbal ingredients on ovarian and cervical carcinoma cell lines. (bvsalud.org)
To investigate cytotoxic activity ofSa-Tri-Lhung-Klod remedy extracts and its herbal ingredients against human ovarian carcinoma cell line (SKOV-3) and cervical adenocarcinoma (HeLa) cell line . (bvsalud.org)
Ethanolic extract ofSa-Tri-Lhung-Klod remedy displayed cytotoxic activity against SKOV-3 and HeLa with IC50 values of 72.84±1.07 and 47.24±2.83 µg/ml, respectively. (bvsalud.org)
However, Caesalpinia sappan, Mammea siamensis and Curcuma comosa showed high cytotoxic activity against SKOV-3 with IC50 values of 9.55±1.38 13.45±0.82 and 14.21±1.30 µg/ml, respectively. (bvsalud.org)
The ethanolic extracts ofCaesalpiniasappan and Mammea siamensis also exhibited cytotoxic activity against HeLa with IC50 values of 6.30±0.06 and 7.72±0.11 µg/ml, respectively. (bvsalud.org)
ART exhibits selective cytotoxic activity against Panc-1, BxPC-3 and CFPAC-1 pancreatic cancer cells with IC(50) values that are 2.3- to 24-fold less than that of the normal human hepatic cells (HL-7702). (medicdebate.org)
The pan caspase inhibitor zVAD-fmk did not inhibit the cytotoxic activity of ART. (medicdebate.org)

The antiproliferative activity exhibited by tuber extracts was not due to necrosis, because the amount of lactate dehydrogenase (LDH) released from cells incubated with the extracts was not significantly different from that released from cells incubated without extracts (control). (psu.edu)

The compounds showed inhibitory activity in a concentration dependant manner. (hindawi.com)
PSSNa) [ 10 ], have shown their inhibitory activity against HSV-1 replication. (molvis.org)
Our results showed that honey, in general, and particularly manuka honey, has potent inhibitory activity against the influenza virus, demonstrating a potential medicinal value. (umf.org.nz)

Furthermore, the viral fluorescence intensities were inhibited by SPS in a dose-dependent manner when the viruses or cells were preincubated with SPS. (molvis.org)
This glycoprotein possesses antimicrobial activity due to its capability to chelate two ferric ions per molecule, as well as to interact with bacterial and viral anionic surface components. (frontiersin.org)
The cationic features of Lf bind to cells, protecting the host from bacterial and viral injuries. (frontiersin.org)
Anti influenza Viral Effect of Honey In Vitro: Potent High Activity of Manuka Honey. (umf.org.nz)
Therefore, the aim of this study was to evaluate the anti- influenza viral activity of honey from various sources. (umf.org.nz)
[ 5 , 6 ] In a localized primary infection, the virus penetrates the mucosal epithelium and invades the cells of the basal layer, where the viral DNA inserts into the host DNA. (medscape.com)

Myeloid angiogenic cells (MAC) derive from hematopoietic stem/progenitor cells (HSPCs) that are mobilized from the bone marrow. (hindawi.com)
Our study is aimed at investigating whether precursors of myeloid angiogenic cells (PACs) treated with known pharmaceuticals would produce media with better proangiogenic activity in vitro and if such media can be used to stimulate blood vessel growth in vivo . (hindawi.com)
The only stimulant that enhanced PAC paracrine angiogenic activity was atorvastatin, which also had ability to stabilize endothelial tubes in vitro . (hindawi.com)
Concluding, high concentrations of atorvastatin stabilize tubes and enhance the paracrine angiogenic activity of human PAC cells in vitro . (hindawi.com)
Such myeloid angiogenic cells (MAC) [ 1 ] of bone marrow origin were known as endothelial progenitor cells (EPCs). (hindawi.com)
Cells previously known as endothelial progenitor cells are considered now to be rather myeloid angiogenic cells than endothelial precursors [ 4 ]. (hindawi.com)
Therefore, the cells we used should be considered as hematopoietic precursors of myeloid angiogenic cells and are referred as PACs. (hindawi.com)

The dose predicted by the 2D model aligns better with the approved clinical dose and the prediction is robust under variations in the in vitro to in vivo translation assumptions. (springer.com)
The two dosing modes yielded similar dose-dependent hormonal responses of H295R cells. (nih.gov)
We identified that SPS reduced the replication of HSV-1 in HCE-T and Vero cells in a dose-dependent manner. (molvis.org)
Our study presented for the first time an active loading method for imatinib and suggests that the optimized liposomal formulation co-encapsulates both drugs with high encapsulation efficiency (>95%), shows enhanced drug retention under tested conditions and delivers a drug:drug ratio capable of improving tumor cell growth inhibition with a mitoxantrone dose reduction of 2.6-fold as compared to single liposomal formulation. (ingentaconnect.com)
Exposure of Capan-2 cells to the aqueous extract of Triphala for 24 h resulted in the significant decrease in the survival of cells in a dose-dependent manner with an IC50 of about 50 μg/ml. (biomedcentral.com)

In our study, we worked on cells derived from the CD34 + hematopoietic stem and progenitor cells, enriched with CD133 + population, and used culture media promoting growth of hematopoietic progenitors. (hindawi.com)
In order to further understand the potential use of MSCs to treat dogs with neurological disorders, this study examined the paracrine action of adipose-derived canine MSCs on neuronal and endothelial cell models. (aston.ac.uk)
Hence, this study has demonstrated that adipose-derived canine MSC CM stimulated neuronal and endothelial cells probably through the paracrine activity of MSC-secreted factors. (aston.ac.uk)
In this study, the radiolabeling efficiency, in vitro stability, and biodistribution of immunoconjugates with eight different bifunctional chelators labeled with 64 Cu were compared. (acs.org)
This in vitro study investigated the effects of implant material composition and surface roughness on the early behaviour of three transformed human osteoblast-like cell lines, MG-63, HOS and U-2 OS, cultured on commercially pure titanium and zirconium substrates of different surface roughness. (ucl.ac.uk)
The experiments indicate that the cell lines may not discriminate between the surface chemistry and topography of the metals used in the study. (ucl.ac.uk)
Together with gene regulation associated with hypoxia in ADSCs, this study has provided several strong leads for a stem cell-derived approach to TM wound healing. (oslomet.no)
The aim of this work was to study the protective activity of a honey of multifloral origin, standardized for total antioxidant power and analytically profiled (HPLC-MS) in antioxidants, in a cultured endothelial cell line (EA.hy926) subjected to oxidative stress. (researchgate.net)
The current study aimed to assess in vitro-anti-sickling activity of Hyphaene thebaica ( H thebaica) (Doum) fruit. (researchsquare.com)
Blood samples used in the evaluation of the anti-sickling activity of Hyphaene Thebaica Fruits extract in this study was taken from patients known to had Sickle cell disease (HB -SS ) attending the Sickle Cell Clinic in Khartoum state. (researchsquare.com)
This study addresses effects on EDCs on adjacent prepubertal Sertoli TM4 cells, specifically on 1) testicular gap junctional intercellular communication (GJIC), one of the hallmarks of non-genotoxic carcinogenicity, 2) GJIC building blocks connexins (Cx), and 3) mitogen-activated protein kinases MAPKs. (muni.cz)
In a recent study, scientists showed that human colon cancer cells die when they are exposed to the approximate number of bioactive compounds present in one cup of mate tea, which has long been consumed in South America for its medicinal properties. (sciencedaily.com)
In the in vitro study, de Mejia and former graduate student Sirima Puangpraphant isolated, purified, and then treated human colon cancer cells with caffeoylquinic acid (CQA) derivatives from mate tea. (sciencedaily.com)
This in vitro study was published in Molecular Nutrition & Food Research , vol. 55, pp. 1509-1522, in 2011. (sciencedaily.com)
An essential oil in cinnamon called cinnamaldehyde can target your fat cells and make them burn more energy, according to a lab study. (webmd.com)
In this study, we base our research on the plant metabolite pulchrol, a natural benzochromene which has been shown to possess antiparasitic activity against Trypanosoma and Leishmania species. (lu.se)
In a recent study, we investigated how changes in the benzyl alcohol functionality affected the antiparasitic activity, but the importance of B- and C-ring substituents is not understood. (lu.se)

dexpanthenol 5% and polyvinyl alcohol accelerated the wounding of corneal cells in vitro. (unifi.it)

The main finding was that POPs at the levels at which they are found in humans can interfere with steroidogenesis in a human adrenocortical cell line. (nih.gov)
The sialyltransferase ST6GAL1 that adds α2-6 linked sialic acids to N-glycans of cell surface and secreted glycoproteins is prominently associated with many human cancers. (nature.com)
We investigated in vitro the paracrine effects of human adipose-derived stem cells (ADSCs) on wound healing mechanisms for human tympanic membrane-derived keratinocytes (hTM) and immortalized human keratinocytes (HaCaT). (oslomet.no)
Each of these infectious diseases is designated by the major target cell: human monocytic ehrlichiosis is caused by Ehrlichia chaffeensis, and human granulocytic ehrlichiosis by an E. equi -like organism. (cdc.gov)
Herein we report the identification of artesunate (ART), a derivative of artemisinin, as a potent and selective antitumor agent against human pancreatic cancer cells in vitro and in vivo. (medicdebate.org)
The caffeine derivatives in mate tea not only induced death in human colon cancer cells, they also reduced important markers of inflammation," said Elvira de Mejia, a U of I associate professor of food chemistry and food toxicology. (sciencedaily.com)
Lymphocyte activation gene-3 (LAG-3) is a coinhibitory receptor associated with impaired T-cell function and is frequently coexpressed with programmed cell death protein-1 (PD-1) in the context of human cancers. (aacrjournals.org)

Electron microscopy of ART-treated cells revealed severe cytoplasmic swelling and vacuolization, swollen and internally disorganized mitochondria, dilation (but not fragmentation) of the nuclei without chromatin condensation, and cell lysis, yielding a morphotype that is typical of oncosis. (medicdebate.org)
Histologically, ALCL is characterized by sheets of large pleomorphic cells with abundant cytoplasm, horseshoe- or wreath-shaped nuclei, and multiple prominent nucleoli. (medscape.com)

The well-known antiviral activity of Lf has been demonstrated against DNA, RNA, and enveloped and naked viruses and, therefore, Lf could be efficient in counteracting also SARS-CoV-2 infection. (frontiersin.org)
From this group, 26 drugs were deemed to have antiviral activity against FIPV. (vin.com)

The formation of this trimolecular complex (i.e. trimer) mimics the immune synapse, leading to therapeutic-dependent T-cell activation and killing of tumor cells. (springer.com)
Due to the high mortality rate of sickle cell patients, previous studies have been indicated that some medicinal plants have shown an anti-sickling activity, which indicates a new therapeutic way to manage people who are affected by these disorders. (researchsquare.com)
The examination of women for the activation of NK, allows specialized scientists to apply a targeted therapeutic approach in order to regulate the function of NK cells. (articlebiz.com)
Taken together, the pharmacologic activity of TSR-033 demonstrates that it is a potent anti-LAG-3 therapeutic antibody and supports its clinical investigation in cancer patients. (aacrjournals.org)

Native honey (1% w/v pH 7.4, 10(6) cells) showed strong quenching activity against lipophilic cumoxyl and cumoperoxyl radicals, with significant suppression/prevention of cell damage, complete inhibition of cell membrane oxidation, of intracellular ROS production and recovery of intracellular GSH. (researchgate.net)
Exosomes are a type of membrane vesicles secreted by living cells. (dovepress.com)
EDCs rapidly dysregulated GJIC in Sertoli TM4 cells mainly via MAPK p38 and/or Erk1/2 pathways by the intermediate hyper- or de-phosphorylation of Cx43 (Ser368, Ser282) and translocation of Cx43 from the plasma membrane, suggesting disturbed intracellular trafficking of Cx43 protein. (muni.cz)
The ART-treated cells exhibited a loss of mitochondrial membrane potential (DeltaPsim) and ART-induced cell death was inhibited in the presence of the reactive oxygen species (ROS) scavenger N-acetyl-cysteine (NAC). (medicdebate.org)
Indeed, OEO/thymol increased the Reactive Oxygen Species (ROS) level leading to mitochondrial membrane potential (MMP, ΔΨm) loss, caspase-3 activation and DNA damage caused S-phase cell cycle arrest. (irost.org)
Yoda1, a specific PIEZO1 agonist, activates a small membrane current and thereby triggers β-cell electrical activity with resultant stimulation of Ca 2+ -influx and insulin secretion. (lu.se)
This paradox is resolved by our finding that PIEZO1 translocates from the plasmalemma into the nucleus (where it cannot influence the membrane potential of the β-cell) under experimental conditions emulating T2D (high glucose culture). (lu.se)
Anaplastic large cell lymphomas (ALCLs) are distinguished from other lymphomas by their anaplastic cytology and constant membrane expression of the CD30 antigen (an activation marker for B or T cells). (medscape.com)

The in vitro antimicrobial activity was tested on Staphylococcus aureus, Methicillin-Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, Streptococcus pneumoniae, Streptococcus pyogenes and Streptococcus mitis. (unifi.it)

Tumor-native ST6GAL1 promotes tumor cell behaviors such as invasion and resistance to cell stress and chemo- and radio-treatments. (nature.com)
The impact of this non-canonical extrinsic mechanism of ST6GAL1 on tumor cell pathobiology is not known. (nature.com)

In vitro experimental analyses demonstrated that sorafenib indirectly inhibited HDAC activity in both sorafenib-sensitive and -resistant HCC cells. (oncotarget.com)

Although long-term repopulating hematopoietic stem cells (HSC) can self-renew and expand extensively in vivo, most efforts at expanding HSC in vitro have proved unsuccessful and have frequently resulted in compromised rather than improved HSC grafts. (lu.se)
article{ff9b980d-2e89-453c-b22b-428d2e903c6f, abstract = {{Although long-term repopulating hematopoietic stem cells (HSC) can self-renew and expand extensively in vivo, most efforts at expanding HSC in vitro have proved unsuccessful and have frequently resulted in compromised rather than improved HSC grafts. (lu.se)
Mesenchymal stem cells (MSCs) have been used in cell replacement therapies for connective tissue damage, but also can stimulate wound healing through paracrine activity. (aston.ac.uk)
Stem Cells Dev, 26(6), 405-418. (oslomet.no)
Fibroblast stem cells or stemlike cells (FSCs) are proposed to play a pivotal role in extracellular matrix (ECM) regeneration by serving as a key source of ECM-producing fibroblasts. (cdc.gov)

3] So that the higher antioxidant property of an antisickling agent enables it to reduce oxidative stress that contributes to sickle cell crisis. (researchsquare.com)
doum fruit extracts contain high amount of flavonoids, phenols used as antioxidant and antibacterial activities which can alleviate the adverse effects of oxidative stress. (researchsquare.com)
The antioxidant, antidiabetic, anticholinesterase and in vitro antitumor activities of AG extracts were recently reported. (unicatt.it)
Different polyphenol, flavonoid and terpenoid amount, and antioxidant activity were found among all samples. (unicatt.it)
Above mentioned effects were significantly blocked when the cells were pretreated with an antioxidant N-acetylcysteine (NAC), suggesting the involvement of ROS generation. (biomedcentral.com)

Experiments with endothelial cells fortified with the isolated fraction from native honey enriched in antioxidants, exposed to peroxyl radicals from 1,1-diphenyl-2-picrylhydrazyl (AAPH, 10 mM) and to hydrogen peroxide (H2O2, 50-100 microM), indicated that phenolic acids and flavonoids were the main causes of the protective effect. (researchgate.net)
Additionally, in-vitro experiments, indicated that OEO/thymol interacts with DNA via minor grooves confirmed by docking method. (irost.org)

To potentiate the neuroprotective effect of HN, we synthesized a hybrid peptide named Colivelin composed of activity-dependent neurotrophic factor (ADNF) C-terminally fused to AGA-(C8R)HNG17, a potent HN derivative. (jneurosci.org)

A hallmark of renal cell carcinoma RCC) invasion is its ability to degrade ECM by local production of gelatinase enzymes. (drrathresearch.org)
Photoactivation of AKT inhibited the transcriptional activity of Forkhead box transcription factor O1 (FoxO1), reducing expression of lipolytic enzymes and FFA generation and release. (google.com)
1975. [Effect of methyl parathion or zineb administration on the activity of some hepatic enzymes in rats]. (cdc.gov)
Ultimately cancer cells died with the induction of two specific enzymes, caspase-3 and caspase-8, de Mejia said. (sciencedaily.com)

Preliminary In Vitro and In Vivo Evaluation of Antidiabetic Activity of Ducrosia anethifolia Boiss. (hindawi.com)
There have been little detailed phytochemical reports on this genus and the antidiabetic activity of this plant is not yet evaluated. (hindawi.com)
Preliminary in vitro evaluation of the antidiabetic activity of crude extract and its furanocoumarins was carried out ( α -amylase, α -glucosidase, and β -galactosidase). (hindawi.com)
Herein, we investigated the phytochemical constituents of D. anethifolia and the potential antidiabetic activity of its crude extract and its major isolated linear furanocoumarins. (hindawi.com)

PMA and TNF-α, with increased concentration, increased MMP-9 secretion, while IL-1ß and LPS did not significantly modify MMP-9 activity. (drrathresearch.org)
Among them, AGA-(C8R)HNG17, one of the most potent derivatives of HN, completely suppresses neuronal cell death by AD-relevant insults at a concentration of 10 p m in vitro . (jneurosci.org)
This activity varied according to concentration (100/500 μg/ml), multiplicity of infection (0.1/0.01), and cell type (Vero E6/Caco-2 cells). (frontiersin.org)

One may speculate that more mature MAC would have better paracrine proangiogenic activity than PAC. (hindawi.com)

There are also some combination therapies that are possible, and they may prove to be more effective in live clinical trials than they were in these cell populations. (vin.com)

Objective: To investigate the effect of sodium polyanethol sulfonate (SPS) on herpes simplex virus type 1 (HSV-1) infection in vitro. (molvis.org)
Fourteen drugs were carried into the advanced studies involving infection of kidney cells. (vin.com)

The effects of 1 × 10-6 M exogenous 2-methoxyestradiol (2 ME) were determined on cell morphology and cell division cycle (Cdc) 2 kinase activity in SNO oesophageal carcinoma cells. (up.ac.za)
Eliminating ROS elimination or inhibiting AKT blocked the effects of the laser therapy in vivo and in vitro. (google.com)
Our previous In Vitro studies demonstrated that simultaneous combination of imatinib with mitoxantrone yielded additive growth inhibition effects against PC-3 cell line. (ingentaconnect.com)
Therefore, we used OEO and its main component, thymol, to explore their effects on cell growth inhibition and anticancer activity. (irost.org)

In vitro studies revealed a 5a-reductase inhibition activity of 1:5600 compared to finasteride but is at minimum 1:18000 in vivo. (wikipedia.org)

A purely mechanistic theory of the cell-substrate interactions may not be sufficient to account for the variations in the reported behaviour of cells grown on different substrates. (ucl.ac.uk)
In studies using animals or cells grown in labs, cinnamon has shown promise for its ability to slow cancer growth and even kill tumor cells. (webmd.com)
Feline kidney cells were grown in the lab and infected with FIPV serotype II. (vin.com)

1 , 2 In recent years, mammalian cell-derived exosomes (a type of extracellular vesicle) have been widely used in the field of biomedicine, including diagnosis, regulation of disease progression and drug delivery. (dovepress.com)

Predicting nanotube fibrogenicity through stem cell-mediated fibroblast focus and spheroid formation. (cdc.gov)

Humanin (HN) is a short bioactive peptide abolishing neuronal cell death induced by various familial AD (FAD)-causative genes and amyloid-β (Aβ) in vitro . (jneurosci.org)
It has been generally accepted that increased levels of toxic amyloid-βs (Aβs) are closely related to AD pathogenesis, including neuronal cell death (for review, see Hardy and Selkoe, 2002 ). (jneurosci.org)

However, the features initially ascribed to endothelial progenitor cells, i.e ., phenotype, expression of endothelial markers, and ability to form tube-like structures on Matrigel, can be mimicked by blood monocytes [ 3 ]. (hindawi.com)
During Central Nervous System ontogenesis, myelinating oligodendrocytes (OLs) arise from highly ramified and proliferative precursors called oligodendrocyte progenitor cells (OPCs). (nature.com)
Myelinating OLs originate from parenchymal precursors expressing the neuron-glia antigen 2 (NG2) chondroitin sulphate proteoglycan and the platelet derived growth factor receptor alpha (PDGFRα), commonly referred to as oligodendrocyte progenitor cells (OPCs) 4 . (nature.com)

If we can reduce the activity of NF-kappa-B, the important marker that links inflammation and cancer, we'll be better able to control the transformation of normal cells to cancer cells," she added. (sciencedaily.com)

Organisms1

Diseases2

Chemicals and Drugs3

Information Science1

Assays10

Cytotoxicity15

Apoptosis16

Assay10

Differentiation in vitro1

Proliferation13

Efficacy4

Methods3

Inhibits2

Cultures4

Toxicity3

Viability2

20222

Silico1

Antagonist1

Fertilization1

Characterization1

Cellular3

Antitumor activity2

Epithelial cells4

Cytotoxic activity7

Lactate dehydrogenase1

Inhibitory activity3

Viral6

Angiogenic7

Dose5

Study17

Corneal1

Human7

Nuclei2

Antiviral activity2

Therapeutic4

Membrane8

Antimicrobial1

Tumor cell2

Analyses1

Stem cells5

Antioxidant5

Experiments2

Neurotrophic1

Enzymes4

Antidiabetic4

Concentration3

Paracrine1

Clinical1

Infection2

Effects4

Studies revealed1

Grown3

Mammalian1

Fibroblast1

Neuronal cell2

Progenitor cells3

Inflammation1