• Lipase from Candida antarctica immobilized on an acrylic resin (Novozym 435) was employed as a biocatalyst. (go.jp)
  • Stability of lipases from Pseudomonas fluorescens (Amano AK), Burkholderia cepacia (Amano PS) and Candida antarctica B (Cal B), immobilized by sol-gel entrapment and sol-gel entrapment combined with adsorption on Celite 545, was investigated by long-term incubation in seven different solvents. (upt.ro)
  • In the present study, immobilisation of Candida rugosa (CRL) lipase onto amino-activated mica is performed via covalent bonding (namely Amino-CRL) and the cross-linking of lipases into nano-reactors through physical adsorption (namely NER-CRL). (researchgate.net)
  • We have immobilized Candida antarctica lipase B (CALB) on different resins in order to obtain a more cost-effective biocatalyst, i.e. to find the cheapest preparation per catalytic activity, for one esterification and one amidation reaction. (lu.se)
  • Spent coffee grounds were treated to obtain suitable carriers for both physical and covalent immobilization of Candida rugosa lipase. (uniroma1.it)
  • A Rh(NHC) phosphonate complex reacts with the lipases cutinase and Candida antarctica lipase B resulting in the first (soluble) artificial metalloenzymes formed by covalent active site-directed hybridization. (ward-lab.ch)
  • In this paper, we describe a simple alternative enzymatic process, in which castor oil is directly converted to an estolide mixture by Candida antarctica lipase A (CALA) catalyzed transesterification. (mdpi.com)
  • We have recently reported on a novel immobilization method for Candida antarctica Lipase B on fumed silica to improve the enzymatic activity in hexane. (inrae.fr)
  • Besthway Immobilized Lipase is developed by Besthway Technology with modern genetic engineering, microbe fermentation and advanced post-process technology, which can efficiently catalyze the ester synthesis and transesterification reactions in the biodiesel production process, and can also be used in other oil and grease processing industries, with the advantages of mild reaction conditions, environmental friendliness, high reusability, and good stability. (foodgradeenzyme.com)
  • In the case of Cal B lipases the operational stability was excelent as well, the transesterification activity remaining unchanged during 14 reuse cycles. (upt.ro)
  • Lipase-catalyzed transesterification (also esterification) from oils or fats for biodiesel production has some advantages over chemical-catalyzed transesterification, such as environmental friendship, lower energy consumption3being widely suitable to low quality oils or fats. (confex.com)
  • Lipozyme, a registered trademark of Novo Nordisk A/S Corp., is a class of industrial enzymes, specifically: lipases. (wikipedia.org)
  • The activity of the immobilized biocatalysts was not significantly altered by the tested organic solvents, while the enantioselectivity values of all immobilized enzymes were higher compared to the native ones. (upt.ro)
  • For the free and immobilized system, the enzymes retained 32% and 92% of their initial activities, respectively, at the end of 48 days of storage. (gazi.edu.tr)
  • The use of hydrophobic ionic liquids enables high specific activities of the enzymes to be obtained, for all types of techniques. (encyclopedia.pub)
  • The inhibitors are perfectly suited for quality control of hydrolytic enzymes like lipases and esterases. (pharma-industry-review.com)
  • No significant losses in enzyme activity or changes in fatty acid selectivity on DAG synthesis were observed during the five pilot productions. (upm.edu.my)
  • The optimized immobilized biocatalyst obtained by adsorption mechanism showed up to 33 U∙g-1 of immobilized enzyme activity, and a higher pH, thermal, and storage stability than the free lipase. (uniroma1.it)
  • The enzyme activities of recombinant strains over-expressing PDI and CPR5 reached the highest levels of 259.47 U/mL and 161.34 U/mL, 635% and 357% higher than the original enzyme activity, respectively. (inra.fr)
  • Transcriptomic analysis of the two recombinant strains in comparison with the control strain showed that the correct folding of proteins assisted by molecular chaperones played a key role in the improvement of FumDM expression and its enzyme activity. (inra.fr)
  • Caspase-3 activity was expressed as the percentage of enzyme activity compared with the control [14]. (egfrsignals.com)
  • An immobilized Rhizomucor miehei lipase had the greatest ester synthesis activity but had comparatively limited hydrolytic activity. (ipb.ac.id)
  • The immobilized lipase exhibited thermal and pH stability, which is not possible with the free lipase. (eurekaselect.com)
  • Today, immobilization is not just only for the initial "recovery" purpose, with proper design, immobilization is an efficient way to improve the enzymatic features, including activity, selectivity, stability, or resistance to inhibitors and chemical reagents. (frontiersin.org)
  • However, the low stability of the lipase due to alcohol (methanol) has been being regarded as the main hurdle to the industrialization of lipase-catalyzed biodiesel production. (confex.com)
  • A lot of research focused on how to improve the specific activity or stability of lipase. (confex.com)
  • Hydrolytic activity, thermal and solvent stability, and sugar ester synthesis were evaluated with those catalysts. (pucv.cl)
  • The modification process imparted switchable wettability to the surface while maintaining the thermal stability and biocompatibility of the CF. During the ethanol transformation, the rapid, reversible cell adsorption and detachment of Saccharomyces cerevisiae were performed through the modulation of wettability, displaying the enhancement of immobilized biomass and immobilization efficiency from 2.20 g/L and 59.43% to 2.81 g/L and 93.32%, respectively. (edu.au)
  • The objective of this work was contributed in developing techniques to obtain immobilized biocatalysts with low cost and high catalytic activity to be applied in the biodiesel synthesis by ethanol route. (usp.br)
  • The immobilized biocatalysts were incubated in various organic solvents for 7 days, at 25 C. The activity and enantioselectivity were assayed in the acylation reaction of 2-hexanol with vinyl acetate. (upt.ro)
  • A novel ruthenium phosphonate inhibitor was synthesized and covalently anchored to a lipase immobilized on a solid support (CALB, Novozym® 435). (ward-lab.ch)
  • Lipase CALB is immobilized on a solid support (Novozym®435). (ward-lab.ch)
  • Immobilization of lipases has been recognized as an efficient way to recycle lipases and enhance production efficiency. (frontiersin.org)
  • The catalytic potential of the immobilized lipase was evaluated in hydrolytic reactions using p-nitrophenol palmitate as substrate. (eurekaselect.com)
  • Ten commercial sources of lipases were evaluated for comparative hydrolytic (in an aqueous emulsion) and ester synthesis (in microaqueous media) activities. (ipb.ac.id)
  • The phosphonate-based molecules are available with either chromogenic (p-nitrophenyl derivatives) or fluorogenic (4-methyl-umbelliferyl derivative) groups for sensitive detection of hydrolytic activity of lipases and esterases. (pharma-industry-review.com)
  • Extending this work, immobilised lipases have novel capabilities in the synthesis of sugar esters. (researchgate.net)
  • Lipase-catalyzed synthesis of sugar esters, as lactulose palmitate, requires harsh conditions, making it necessary to immobilize the enzyme. (pucv.cl)
  • This solid biocatalyst also preserved 100 % of its activity after four recycles in p-nitrophenyl palmitate hydrolysis in hexane medium and permitted an almost 60 % conversion of milk fats in fatty acids after 18 h of reaction, maintaining this value for three reuses. (uniroma1.it)
  • Regioselective Enzymatic Hydrolysis of Acetylated Pyranosides using Immobilised Lipases. (unipv.it)
  • The microbial lipases offered by EUCODIS Bioscience cover a wide range of substrates and are appropriate for hydrolysis in aqueous solutions and esterifications in organic solvents. (pharma-industry-review.com)
  • It was found that lipase immobilized on macroporous polypropylene (Accurel MP1000) and polymethylmethacrylate (Lewatit) provided biocatalyst preparations where the cost of enzyme and carrier was significantly less than the cost of commercially available Novozym (R) 435, indicating a potential for decreased cost. (lu.se)
  • Protein immobilization, via covalent bonding or physical adsorption on the support, ensures high stabilities and activities. (encyclopedia.pub)
  • For industrial purposes, it can be immobilized on macroporous ion-exchange resins. (wikipedia.org)
  • Therefore, a study was conducted to evaluate the effect of different chemical surfaces of hierarchical meso-macroporous silica in the immobilization of two lipases from Pseudomonas stutzeri (PsL) and Alcaligenes sp. (pucv.cl)
  • With a very high molar proportion of fatty acid to saccharide when compared to the other studies, the authors suggested that increasing the proportion of fatty acid could negatively affect the lipase conformation due to an acidification of the reaction medium. (researchgate.net)
  • For the five most effective ester synthesizing lipases, each had a characteristic optimum water content for maximum initial reaction rates. (ipb.ac.id)
  • The reaction mixture is analyzed by NMR to determine the estolide number (EN) and MALDI MS to identify individual components, in addition to titration to determine the acid value (AV). Estolide trimers and tetramers (EN 2-3) were formed over 24 h in a system with 2:1 ( v/v ) castor oil-water. (mdpi.com)
  • subsequent direct binding to EziG from crude extracts resulted in an immobilised catalyst with superior activity to Novozym 435. (almacgroup.com)
  • Each immobilization method was investigated to obtain the best insoluble biocatalyst immobilized activity, efficiency, and recovery. (uniroma1.it)
  • Several compounds have been added to the culture medium in order to assess their efficiency as inducers of lipase production. (scialert.net)
  • Based on the accumulation of the biofilm, the fabrication and utilization of PNIPAM-CF improved the efficiency of continuous immobilized fermentation, making the ethanol production reach 26.34 g/L in the sixth batch of fermentation. (edu.au)
  • It involves lipase-catalyzed acylation of betulinic acid with anhydrides as acylating agents in organic solvent. (go.jp)
  • The immobilized bifunctional catalytic system showed activity in both racemization of (S)-1-phenylethanol and selective acylation of 1-phenylethanol. (ward-lab.ch)
  • Some researchers use the free lipase (which is cheaper) as catalyst, but do not recycle lipase, other researcheres use and recycle immobilized lipase which is much expensive. (confex.com)
  • The production of extra cellular lipase in submerged fermentation of Yarrowia lipolytica NCIM 3589 has been investigated. (scialert.net)
  • Biofilm-mediated continuous fermentation with cells immobilized has gained much attention in recent years. (edu.au)
  • Specific activities for Amino-CRL and NER-CRL increased by 2.4 and 2.6-fold, respectively, upon immobilisation. (researchgate.net)
  • Biological applications with a focus on permeability enhancing, antimicrobial activity, and antibiofilm properties of 6′-lactose-based esters are also reported. (researchgate.net)
  • The depigmenting effect of kojic acid and kojic acid esters was evaluated by the inhibitory effect of melanin formation and tyrosinase activity on alpha-stimulating hormone- (α-MSH-) induced melanin synthesis in B16F1 melanoma cells. (annahealth.com)
  • Kojic acid and kojic acid esters also show antioxidant activity that will enhance the depigmenting effect. (annahealth.com)
  • Lipases like Lipozyme and Novozyme (reg.trademark by Novozymes) play a big role in the synthesis of biodiesel. (wikipedia.org)
  • Immobilization by covalent bonding on sepharose and silica afforded a maximum of 380 and 40 IU/g activity, respectively,whereas sol 13gel entrapment provided a maximum of 150 IU/g activity in dry powder. (csircentral.net)
  • Methanol was initially added to the reactors in order to increase the microbial activity of the sludge, except for the last one, in order to compare the effect of methanol on start-up. (ijche.com)
  • EUCODIS Bioscience offers a broad range of microbial lipases for the pharmaceutical industry. (pharma-industry-review.com)
  • EUCODIS has a collection of over 70 advanced lipases of microbial origin, which are specifically chosen and developed for applications in the pharmaceutical industry, as well as the cosmetics and fine chemical industries. (pharma-industry-review.com)
  • COS are known to have eliciting activities leading to a variety of defence responses in plants in response to microbial infections to reduce the negative impact of diseases on yield and quality of crops. (medcraveonline.com)
  • A series of Grubbs‐type catalysts that contain lipase‐inhibiting phosphoester functionalities have been synthesized and reacted with the lipase cutinase, which leads to artificial metalloenzymes for olefin metathesis. (ward-lab.ch)
  • Therefore, screening of different chemical surfaces on porous silica used as supports for lipase immobilization allowed obtaining active and stable biocatalyst to be employed in the novel synthesis of lactulose palmitate. (pucv.cl)
  • Also, its activity was far higher than the free lipase. (eurekaselect.com)
  • The temperature range generally oscillates between 40 °C and 60 °C, a characteristic interval of the immobilized form, which usually guarantees better conditions because it is more stable than the free form. (researchgate.net)
  • C, a characteristic interval of the immobilized form, which usually guarantees better conditions because it is more stable than the free form. (researchgate.net)
  • This work presents a novel process in which both free and immobilized lipase are applied and successfully recycled. (confex.com)
  • The free lipase will first catalyze more than 90% of the conversion of oils and the obtained products contain still free fatty acid (FFA) which is not accepted by the standard specification of biodiesel. (confex.com)
  • Compared to the process using only free lipase (normally a neutralization with alkaline needed to remove FFA), the novel process can directly get the qualified products and higher yield because of the conversion of FFA. (confex.com)
  • Compared to the process using only immobilized lipase, the dosage of immobilized lipase can be significantly reduced with the novel process because that less than 10% of the conversion is left for the immobilized enzyme after the first conversion with free lipase. (confex.com)
  • K-m values of free and immobilized lipases were found to be 0.290, 0.422 mM while V-max values were calculated as 0.089, 0.080 mM.min(-1), respectively. (gazi.edu.tr)
  • Recently, reports have emerged detailing lipase-catalyzed synthesis of estolides from free hydroxy fatty acids. (mdpi.com)
  • PsL and AsL were immobilized in octyl (OS), glyoxyl (GS), and octyl-glyoxyl silica (OGS). (pucv.cl)
  • The activity assay of the immobilized lipase was carried out by the standard assay method. (eurekaselect.com)
  • Caspase-3 activity assay The activity of caspase-3 was determined using the Caspase-3 activity Kit (Beyotime Institute of Biotechnology, Jiangsu, China). (egfrsignals.com)
  • Results: Immobilization enhanced the lipase activity and the structure of the nanogels made significant difference in enhancing their activity as the lipase immobilized on poly(Acryl azide)-cl-divinylbenzene nanogel exhibited the higher activity than the lipase immobilized on the precursor nanogel. (eurekaselect.com)
  • It is based on the utilization of filamentous fungi whole cells with high lipase activity (glycerol ester hydrolase - E.C.3.1.1.3) immobilized in suitable support. (usp.br)
  • AsL), which exhibit esterase activity. (pucv.cl)
  • Predictive quantitative structure-activity relationship modeling of the antifungal and antibiotic properties of triazolothiadiazine compounds. (usda.gov)
  • This method was applied to the conjugation of biotin and β-linked galactose residues to yield an enzymatically active thermophilic lipase, which revealed specific binding to Erythrina cristagalli lectin by SPR binding studies. (beilstein-journals.org)
  • The well characterized precursor nanogel and its functional derivatives were used as supports to immobilize lipase (triacyl glycerin). (eurekaselect.com)
  • The anticancer activity of betulinic acid and its 3- O -acylated derivatives were then evaluated in vitro against human lung carcinoma (A549) and human ovarian (CAOV3) cancer cell lines. (go.jp)
  • Chitin and its derivatives have become a promising alternative treatment due to its natural character, antifungal activity and elicitation of defense responses in plant tissue. (medcraveonline.com)