Activity hek293 cells. Medical search. Frequent questions

OATP2B1 variants were expressed in human derived HEK293 cell line using baculovirus expression system. (helsinki.fi)

For this purpose, we established stable recombinant HEK-293 cell lines expressing ERα fused with emerald luciferase. (nih.gov)
Here we assessed electrophysiologically the positive modulatory activity of humulone on recombinant GABAreceptors expressed in HEK293 cells. (greenmedinfo.com)
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)

After expression of GPR40-Gα q in HEK293 cells and membrane preparation basal binding of [ 35 S]GTPγSinGα q immunoprecipitates was high and not elevated substantially by fatty acids. (aspetjournals.org)
Investigating the role of cell membrane proteins has increased over the last decade, as drugdrug interactions and genetic polymorphisms have been found to cause changes in drug pharmacokinetics and dynamics. (helsinki.fi)
Very lipophilic drugs enter the cell membrane through passive diffusion, but polar or ionized organic compounds can enter the cell membrane only by transporters. (helsinki.fi)
We propose that GPVI may be expressed as a monomer on the cell surface and it forms dimers in the membrane through diffusion, giving rise to a mixture of monomers and dimers. (nih.gov)

The high apparent constitutive activity of GPR40-Gα q was also reversed by a small-molecule GPR40 antagonist, and basal [ 35 S]GTPγS binding was prevented by the selective Gα q /Gα 11 inhibitor YM-254890. (aspetjournals.org)
Antagonist activity at human CysLT2 expressed in HEK293 cells assessed as inhibition of LTD4-induced calcium mobilization preincubated for 30 mins pr. (bindingdb.org)

The expression of caspase-3 and caspase-9 genes increased in HCT-116 cell lines after 48 h of treatment, suggesting cell death by the apoptotic pathways. (mendeley.com)
Ubigene exclusive KO Cell Line Bank, over 5000 KO cell lines, covering thousands of genes from 8 popular signaling pathways and nearly 100 diseases. (ubigene.us)

Interferon α 2A (IFN-α 2A) belongs to the type I interferon protein family and is produced by many cell types in response to viral infection. (sigmaaldrich.com)
This was also observed when human embryonic kidney (HEK) 293 cells were transfected with a GPR40-Gα q fusion protein. (aspetjournals.org)
The current studies provide novel insights into the pharmacology of GPR40 and indicate that G protein-coupled receptors which respond to fatty acids, and potentially to other lipid ligands, can be occupied by endogenous agonists before assay and that this may mask the pharmacology of the receptor and may be mistaken for high levels of constitutive activity. (aspetjournals.org)
The encoded protein and its ligand play a role in the development and organization of lymphoid tissue and transformed cells. (wikipedia.org)
Based on the structure, we performed integrative analysis of the cellular trafficking, protein thermostability, ATP hydrolysis, and the transport activity of representative mutations. (elifesciences.org)
This gene encodes a protein with protease activity and is expressed in the placenta. (cancerindex.org)
We evaluated FOXP1 activity by an in vitro luciferase model and assessed protein stability in vitro by western blotting. (bmj.com)
Activity of SOD and POD, as well as the MDA and protein content of the two rice cultivars generally increased with increasing time of exposure to NaCl. (bvsalud.org)

HumanKine IFN-α 2A, expressed in human HEK 293 cells, is a glycosylated monomer with an apparent molecular mass of 16 kDa. (sigmaaldrich.com)
Production in human HEK 293 cells offers authentic glycosylation. (sigmaaldrich.com)
The activity is determined by the dose-dependent cytotoxicity of the human TF-1 cell line (human erythroleukemic indicator cell line)) ≤ 0.4. (sigmaaldrich.com)
HEK-Dual™ cells are derived from the human embryonic kidney 293 (HEK293) cell line through the stable integration of two inducible reporter genes for SEAP (secreted embryonic alkaline phosphatase) and Lucia luciferase. (invivogen.com)
In cells lacking expression of Ca 2+ -mobilizing G proteins, coexpression of human GPR40 and Gα q allowed medium- and long-chain fatty acids to elevate intracellular [Ca 2+ ]. (aspetjournals.org)
2014. Regulation of the human cathelicidin antimicrobial peptide gene by 1α,25-dihydroxyvitamin D3 in primary immune cells. . (oregonstate.edu)
ELISA tests revealed that SVmab was capable of binding to Nav1.7-expressing HEK293 cells, mouse DRG neurons, human nerve tissue, and the voltage-sensor domain II of Nav1.7. (springer.com)
Human Embryonic Kidney cells (HEK) is a human derived mammalian cell-line that is widely used in the study of OATP1B1 transporter. (helsinki.fi)
We found that measurement of NO release by DAF-FM fluorescence in primary human aortic endothelial cells (HAEC) or in AT(2)R-transfected CHO cells is a reliable assay for the characterization of AT(2)R ligands. (mdc-berlin.de)
Promotes neurogenesis in human induced pluripotent stem cells (iPSCs) when used in combination with SB 431542 (Cat. (tocris.com)
DMH-1 induces intestinal differentiation in human intestinal organoids (hIOs) derived from human pluripotent stems cells (hPSCs). (tocris.com)
All cells of human and murine origin in our cell bank have available STR Authentication reports, guaranteeing accurate cell identity. (ubigene.us)
with small or large lateral dimensions) are sensed by primary human macrophages which are key cells of the innate immune system. (cdc.gov)
Here, we use a human cell-based reporter assay to characterize off- target cleavage of Cas9-based RGENs. (cdc.gov)
We readily detected off-target alterations induced by four out of six RGENs targeted to endogenous loci in human cells by examination of partially mismatched sites. (cdc.gov)
Our work demonstrates that RGENs are highly active even with imperfectly matched RNA-DNA interfaces in human cells, a finding that might confound their use in research and therapeutic applications. (cdc.gov)
Recent work has demonstrated that clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems1-3 can serve as the basis of a simple and highly efficient method for performing genome editing in bacteria, yeast and human cells, as well as in vivo in whole organisms such as fruit flies, zebrafish and mice4-13. (cdc.gov)
Although Maraffini and colleagues7 recently performed a systematic investigation of Cas9 RGEN specificity in bacteria, the specificities of RGENs in human cells have not been extensively defined and, to our knowledge, bona fide off-target mutations induced by Cas9 have not been identified in any eukaryotic cell or organism. (cdc.gov)
Understanding the scope of RGEN-mediated off-target effects in human and other eukaryotic cells will be critically essential if these nucleases are to be used widely for research and therapeutic applications. (cdc.gov)
To begin to define the specificity determinants of RGENs in human cells, we sought to perform a large-scale test in which we assessed the effects of systematically mismatching various positions within multiple gRNA/target DNA interfaces. (cdc.gov)
This engineered human IgM constitutively expressed in a HEK-293 stable cell line can replace human-positive control sera in diagnostic serological techniques such as IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA). (cdc.gov)
Compared to the parent murine MAbs, the human-chimeric IgM antibody had identical serological activity to CSGVs in ELISA and demonstrated equivalent reactivity compared to human immune sera in the MAC-ELISA.IMPORTANCEOrthobunyaviruses in the California serogroup cause severe neurological disease in children and adults. (cdc.gov)
The chimeric IgM produced from the stably expressing HEK293 cell line was evaluated for use as a surrogate human-positive control in a serologic diagnostic test. (cdc.gov)

In the present study, we have used the advanced fluorescence microscopy techniques of single-molecule microscopy, fluorescence correlation spectroscopy (FCS) and bioluminescence resonance energy transfer (BRET), and mutagenesis studies in a transfected cell line model to show that GPVI is expressed as a mixture of monomers and dimers and that dimerization through the D2 domain is not critical for activation. (nih.gov)

Membranes of rat INS-1E cells that express GPR40 endogenously provided similar observations. (aspetjournals.org)

Ubigene has modified over 5000 genes from more than 200 cell lines with our exclusive innovation CRISPR-U™ technology. (ubigene.us)
The results showed that HH11 had more favorable adjustment in antioxidant and osmotic activity than IR29 upon exposure to salt stress, and highlighted candidate genes that could play roles in the function and regulation mechanism of salt tolerance in rice. (bvsalud.org)

All the compounds produced a significant reduction in HCT-116 cell line proliferation, except compounds 2 and 6. (mendeley.com)
DMH-1 suppresses lung cancer cell proliferation, migration, invasion in vitro and reduces tumor growth in a mouse lung cancer xenograft model. (tocris.com)

Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. (rndsystems.com)
Determined by its ability to inhibit MC3T3/E1 osteoblasts cell differentiation. (peprotech.com)

The biological activity of VEGF was confirmed by its proliferative effect in HUVEC cells. (ehu.eus)
The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding. (rndsystems.com)
References are publications that support the biological activity of the product. (tocris.com)
The purpose of this review is to provide updated and categorized information on the traditional uses, phytochemistry, biological activities, and toxicological research of Moringa species in order to explore their therapeutic potential and evaluate future research opportunities. (frontiersin.org)
Most of their biological activity is caused by their high content of flavonoids, glucosides, and glucosinolates. (frontiersin.org)
By documenting the traditional uses and biological activities of Moringa species, we hope to support new research on these plants, especially on those species whose biological properties have not been studied to date. (frontiersin.org)

This lack of standard assays has hampered the development of new drugs targeting the AT(2)R. Therefore, we aimed at developing a reliable, technically easy assay for the determination of intrinsic activity of AT(2)R ligands, primarily for distinguishing between AT(2)R agonists and antagonists. (mdc-berlin.de)
d) EMA401 and PD123319, which are commonly regarded as AT(2)R antagonists, are partial agonists at the AT(2)R. Collectively, we have developed and tested an assay based on the measurement and quantification of NO release in HAEC or in AT(2)R-CHO cells that is suitable for the characterisation of novel and established AT(2)R ligands. (mdc-berlin.de)
In HEK 293 cells whilst EP2 exhibited minimal ligand-induced internalisation with all ligands, there was significant constitutive internalisation, which was dynamin-dependent. (endocrine-abstracts.org)

This has suggested a potential role for GPR40 in the regulation of β-cell responsiveness to free fatty acids and hence as a potential therapeutic target in the regulation of insulin secretion and diabetes. (aspetjournals.org)
In both HEK 293 cells and primary myometrial cells from term pregnancies only AH13205 and butaprost were able to increase intracellular Ca2+ levels or PGE2 secretion, and AH13205 increased PGE2 secretion ~40 fold more than butaprost. (endocrine-abstracts.org)
Using THP-1 knockdown cell lines, we found that IL-1beta secretion was caspase-1-, ASC-, and NLRP3- dependent. (cdc.gov)

HEK-Dual™ cells express endogenous levels of various pattern recognition receptors (PRRs), including TLR3, TLR5, NOD1, RIG-I-like receptors ( RLR ), and STING [in-house data]. (invivogen.com)
Because the neurobiological substrate of cariprazine has remained elusive, we took advantage of PharmacoSTORM to provide in vivo evidence that cariprazine predominantly binds to D 3 dopamine receptors on Islands of Calleja granule cell axons but avoids dopaminergic terminals. (nature.com)
Humulone modulation of GABA A receptors and its role in hops sleep-promoting activity. (greenmedinfo.com)

Early blockade of injured primary sensory afferents reduces glial cell activation in two rat neuropathic pain models. (springer.com)

Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™ Solution , a SEAP detection reagent, and QUANTI-Luc™ 4 Lucia/Gaussia , a Lucia luciferase detection reagent. (invivogen.com)

Patch-clamp recordings showed that SVmab, but not rSVmab, markedly inhibited Na + currents in Nav1.7-expressing HEK293 cells. (springer.com)

Brain circuits consist of more than hundred distinct neuronal and glial cell types 1 , which communicate via billions of synapses built up from highly compartmentalized nanoscale signaling platforms 2 . (nature.com)

In addition, after transcription, a wide array of RNA-binding proteins interacts with cis -acting elements located mainly in the 3' untranslated region, determining the fate of mRNAs in eukaryotic cells. (frontiersin.org)

Slit2 is a member of the Slit family that signals through the Roundabout (Robo) receptor as a repellent for axon guidance and neuronal migration, and also acts as a chemoattractant to vascular endothelial cells and a chemotaxis inhibitor for leukocytes. (peprotech.com)

The anticancer activity of these compounds was evaluated in colon cancer, HCT-116, and noncancerous, HEK-293, cell lines using the MTT antiproliferative assays. (mendeley.com)

The caspase-3 and caspase-9 expressions and DAPI (4′, 6-diamidino-2-phenylindole) staining assays were used to evaluate the apoptotic activity. (mendeley.com)
SARS-CoV-2 seroprevalence studies are instrumental in monitoring epidemic activity and require well-characterized, high-throughput assays, and appropriate testing algorithms. (cdc.gov)

IFN-α 2A enhances the expression of MHC proteins by increasing the presentation of antigen peptides and activates cytotoxic T cells. (sigmaaldrich.com)
Alterations occurring in BMPR expression and activity add up to cardiovascular diseases, fibrotic disorders, and skeletal abnormalities. (news-medical.net)
The wild-type SLCO1B1-gene encoding the OATP1B1 was virulent with baculovirus into the cells by the Bac-to-Bac® Baculovirus Expression System. (helsinki.fi)
For expression in the Sf9 cells, the aim of the study was to clone the SLCO1B1-gene into the pFastBac vector. (helsinki.fi)
The expression of OATP1B1 transporter in HEK293 cells was successful. (helsinki.fi)
Exogenous expression of the Arg482 (wild type), Gly482, and Thr482 variants of ABCG2 conferred HEK293 cells resistance to SN-38 by 15.0-, 5.0-, and 5.3-fold, respectively. (oldcitypublishing.com)

Bond and IJzerman, 2006 ), and pharmacological studies based on the dynamics of cellular Ca 2+ mobilization are also well known to be inappropriate to detect such activity. (aspetjournals.org)
CO-expanded nanofiber scaffolds maintain activity of encapsulated bioactive materials and promote cellular infiltration and positive host response. (oregonstate.edu)
When [Arg482]ABCG2-transfected HEK293 cells were incubated with SN-38 in the presence of 20 mM quercetin, cellular resistance to SN-38 was partly reversed. (oldcitypublishing.com)

In the experimental part of this thesis the goal was to express OATP1B1 transporter in Sf9 and HEK293 cell lines. (helsinki.fi)
HEK293 cells expressing OATP1B1 transporter are well suited for the study of the SLCO1B1-gene. (helsinki.fi)
Irinotecan (CPT-11) is a widely-used potent anticancer drug that inhibits mammalian DNA topoisomerase I, however overexpression of the ATP-binding cassette transporter ABCG2 can confer cancer cells resistance to SN-38, the active form of CPT-11. (oldcitypublishing.com)
16-BrSalA increased dopamine transporter (DAT) activity in HEK-293 cells coexpressing DAT and KOR, as well as in rat nucleus accumbens and dorsal striatal tissue. (ku.edu)

Notably, electrical field stimulation increased the blocking activity of SVmab and rSVmab in Nav1.7-expressing HEK293 cells. (springer.com)

The cell lines in our cell bank have been successfully used in gene-editing cell line generation, applicable for all kinds of gene-editing experiments. (ubigene.us)
The subsequent 2,4-substituted CPs exhibited were unreactive towards sulphur nucleophiles while maintaining cytotoxicity against HT-29, MCF-7, NCI-H460, HCT-116 and MDA-MB 231 cells lines. (efmc.info)
We established stable HEK293 cell lines expressing double isoforms, UGT1A1 and UGT1A9, UGT1A4 and UGT1A9, or UGT1A6 and UGT1A9, as well as stable cell lines expressing each single isoform. (aspetjournals.org)

To solve this dilemma, cells must use their own proteins as sources of amino acids in response to the nutritional downshift. (go.jp)
The peptide-based adsorbents were finally utilized to purify AAV2 from a HEK 293 cell lysate, affording high recovery (50-80%), 80-to-400-fold reduction of host cell proteins (HCPs), and high transduction activity (up to 80%) of the purified viruses. (authorea.com)

As a result, these cells allow the simultaneous study of the NF-κB pathway, by monitoring the activity of SEAP, and the IRF pathway, by assessing the activity of the secreted Lucia luciferase. (invivogen.com)
Here, we describe a quantitative, high-throughput, luciferase-based assay for the evaluation of SERDs activity. (nih.gov)

It has antiproliferative and antitumor activities, as well as induction of apoptosis and the inflammatory response. (sigmaaldrich.com)
All the compounds exhibited antiproliferative activity against the HCT-116 cell line with IC50 values at 33 ± 1.25, 36 ± 2.25, 34 ± 2.15, 32 ± 2.35, 34 ± 2.65, and 36 ± 1.95 μg/mL for compounds 1 to 6, respectively. (mendeley.com)

The compound can also be used in protocols for the chemical reprogramming of somatic cells to iPSCs. (tocris.com)

Lymphotoxin beta receptor (LTBR), also known as tumor necrosis factor receptor superfamily member 3 (TNFRSF3), is a cell surface receptor for lymphotoxin involved in apoptosis and cytokine release. (wikipedia.org)
C/EBPα and the Vitamin D Receptor Cooperate in the Regulation of Cathelicidin in Lung Epithelial Cells. (oregonstate.edu)

Results are quantified by ELISA and expressed as mean ± SEM relative to the ligand-binding activity of BMPR2-ECD in the absence of 3F6. (news-medical.net)

Transfection efficiencies of nanoplexes were measured in terms of percentage of GFP expressing cells, mean fluorescent intensity (MFI) and VEGF (ng/ml) production in HEK293, C6 and primary neuronal culture cells. (ehu.eus)

Magnetoplexes showed the highest transfection efficiencies in C6, followed by lipoplexes, and in primary neuronal culture cells, followed by polyplexes. (ehu.eus)

We have recently reported that chronic pain is associated with amplified activity of PB neurons in a rat model of neuropathic pain. (iasp-pain.org)

Although SERDs have been developed for the treatment of ER-positive breast cancers for nearly a decade, their precise mechanism(s) of action and structure-activity relationship are still unclear. (nih.gov)
We also designed and synthesized new diphenylmethane derivatives as candidate SERDs, and evaluated their SERDs activity using the developed system in order to examine their structure-activity relationship, taking EC 50 as a measure of potency, and E max as a measure of efficacy. (nih.gov)
Structure-activity relationship. (nih.gov)

Knockdown of sodium channel Nav1.6 blocks mechanical pain and abnormal bursting activity of afferent neurons in inflamed sensory ganglia. (springer.com)
Here we demonstrate that similar activity amplification occurs in mice, and that this is related to suppressed inhibition to PB neurons from the central nucleus of the amygdala (CeA) in animals of either sex. (iasp-pain.org)
Animals with pain after chronic constriction injury of the infraorbital nerve (CCI-Pain) displayed higher spontaneous and evoked activity in PB neurons, and a dramatic increase in after-discharges-responses that far outlast the stimulus-compared to controls. (iasp-pain.org)
We show that this pathway regulates the activity of pain-related neurons in PB, and that, in chronic pain, this inhibitory pathway is suppressed, and that this suppression is causally related to pain perception. (iasp-pain.org)

However, there is a differential spatial requirement for cAMP, Ca2+ and PGE2 signalling in HEK 293 cells demonstrating the physiological importance of constitutive EP2 trafficking. (endocrine-abstracts.org)

This raises the question whether humulone plays a key role in hops pharmacological activity and potentially interacts with other modulators such as ethanol, bringing further enhancement in GABAreceptor-mediated effects of beer. (greenmedinfo.com)

First, we determined the endotoxin content of all the materials, a prerequisite for any studies using immune-competent cells. (cdc.gov)

Glycosylation contributes to stability in cell growth media and other applications. (sigmaaldrich.com)
Cells must balance energy-efficient growth with the ability to adapt rapidly to sudden changes in their environment. (go.jp)
Once amino acid biosynthetic enzymes start to accumulate, the cell is able to produce its own amino acids, and a new growth phase begins. (go.jp)

The Sf9 cell line is isolated from Spodoptera frugiperda insect and is one of the standard in vitro tools in a genetic engineering study. (helsinki.fi)

U2OS.EGFP cells caused by inactivating frameshift insertion/deletion (indel) mutations introduced by error prone non-homologous end-joining (NHEJ) repair of nuclease-induced double-stranded breaks (DSBs) (Fig. 1a and Methods ). (cdc.gov)

Activity-dependent changes in the density of these signalosomes occur at the nanoscale level and are fundamentally important for physiological and pathological brain plasticity 3 . (nature.com)

Non-viral vectors based on magnetoplexes, lipoplexes and polyplexes for VEGF gene delivery into central nervous system cells. (ehu.eus)
Overall, these results provide new insights for VEGF gene delivery into CNS cells using non-viral vectors. (ehu.eus)
At the same time, we already provide customers with high quality gene-editing tools for cell or animal research worldwide. (ubigene.us)

Moringa species are well-known for their antioxidant, anti-inflammatory, anticancer, and antihyperglycemic activities. (frontiersin.org)

Here, we introduce PharmacoSTORM super-resolution imaging that combines the complementary advantages of these approaches and enables cell-type- and compartment-specific nanoscale molecular measurements. (nature.com)

utilized a cell-free immunoprecipitation assay to analyze the neutralizing potential of the mouse monoclonal antibody 3F6 to hinder the BMPR2-ECD ligand-binding activity. (news-medical.net)
Antibody 3F6 reduces BMPR2-ECD ligand-binding activity in a modified immunoprecipitation assay. (news-medical.net)

DMH-1 is also offered as part of the Tocriscreen 2.0 Max , Tocriscreen Kinase Inhibitor Library and Tocriscreen Stem Cell Library . (tocris.com)

Anatomy8

Organisms1

Chemicals and Drugs39

Analytical, Diagnostic and Therapeutic Techniques and Equipment14

Phenomena and Processes26

Disciplines and Occupations1

Information Science3

Derived HEK293 cell line1

Recombinant3

Membrane4

Antagonist2

Pathways2

Protein8

Human23

Fluorescence1

Membranes1

Genes2

Proliferation2

Differentiation2

Biological6

Ligands3

Secretion3

Receptors3

Blockade1

Reporter1

Currents1

Glial cell1

Eukaryotic cells1

Endothelial cells1

Anticancer activity1

Assays2

Expression6

Cellular3

Transporter4

Stimulation1

Lines3

Proteins2

Luciferase2

Antiproliferative2

Somatic1

Receptor2

ELISA1

Fluorescent1

Neuronal1

Neuropathic pain1

Structure-activity rel3

Neurons4

Constitutive1

Pharmacological1

Immune1

Growth3

Vitro1

Mutations1

Physiological1

Gene3

Antioxidant1

Approaches1

Antibody2

Inhibitor1