Activity enzyme chromogenic compounds. Medical search. Frequent questions

Avicelase has almost exclusively exo-cellulase activity, since avicel is a highly micro-crystalline substrate. (wikipedia.org)
This structure is adapted for working on an insoluble substrate, and it allows the enzyme to diffuse two-dimensionally on a surface in a caterpillar-like fashion. (wikipedia.org)
Chromogenic activity is a result of a chromogenic substrate for the detection of the β-glucosidase enzyme, common to all Listeria spp. (thermalindo.com)
The specific differential activity of this agar is obtained with a proprietary lecithin substrate for the detection of the phospholipase enzyme present in the L. monocytogenes colonies growing on this media. (thermalindo.com)
This enzyme hydrolyzes the chromogenic substrate: TMB, to form a colored compound whose reaction will be stopped by sulfuric acid. (cryopep.com)
The most promising candidates are classified as cutinases (EC 3.1.1.74), but the turnover rates are only low to moderate, reflecting that PET is an unnatural substrate for these enzymes [ 5 ]. (biomedcentral.com)
In addition to possessing identical sequence specificity of cleavage (Ile-Glu-Gly-Arg ) to factor Xa on prothrombin, the macromolecular physiological substrate, notanarin also specifically hydrolyzes a number of factor Xa-specific chromogenic substrates such as CH3SO2-D-leucyl-glycyl-L-arginine-p-nitroanilide and N-benzoyl-L-isoleucyl-L-glutamyl-(piperidyl)-glycyl-L-arginyl-p-nitroanilide hydrochloride. (venomsupplies.com)
The most commonly used methodology is the chromogenic assay, which uses a chromophore-linked substrate of factor Xa. (medscape.com)
Upon cleavage of the substrate by the active enzyme (factor Xa), a colored compound is released. (medscape.com)
Their performance for the detection of Pseudomonas aeruginosa were compared to the lead compound (7-N-(b-alanyl)amino-1-pentylphenoxazin-3-one), the substrate contained in a commercially available medium, chromIDTM ID Ps. (sunderland.ac.uk)
α-Glucosidase (α-D-Glucosidase), a carbohydrate hydrolyzing enzyme, catalyzes the liberation of α-glucose from the non-reducing end of the substrate. (medchemexpress.com)
The activity of this enzyme is commonly assayed using a leucine arylamide chromogenic substrate such as leucyl beta-naphthylamide. (lookformedical.com)
3). Plasmin is supplied in 50% (vol/vol) glycerol/H2O and should be stored at -20°C. Activity is measured by chromogenic substrate assay and purity is judged by SDS gel electrophoresis. (cellsystems.eu)
Specific activity is determined by Chromagenic assay using Spectrozyme-PL (American Diagnostica) as substrate. (cellsystems.eu)
Chromophore-linked substrate (CLS405): probing metallo-β-lactamase activity and inhibition. (ox.ac.uk)
Herein the identification of an inexpensive and stable chromogenic substrate suitable for use in assays of clinically relevant MBLs is described. (ox.ac.uk)

Both binding of substrates and catalysis depend on the three-dimensional structure of the enzyme which arises as a consequence of the level of protein folding. (wikipedia.org)
Display our full list of chromogenic substrates . (megazyme.com)
New chromogenic substrates for the assay of alpha-amylase and (1→4)-β-D-glucanase. (megazyme.com)
With a Δλmax value of 100 nm between the parent and hydrolysis product, a higher analytical accuracy is possible with CLS405 than with commonly used chromogenic substrates. (ox.ac.uk)
Microbial cells have the ability to convert raw materials or substrates into valuable organic compounds, such as butanol, ethanol, and acetone. (pharmacyfreak.com)

In conclusion, our research involves the high-throughput assay for screening small molecules to modulate the AP activity of LTA 4 H. The discovery of small molecules that activate the AP activity of the LTA 4 H enzyme is a potential new therapeutic agent for lung inflammatory diseases, such as chronic obstructive pulmonary disease (COPD). (gmu.edu)
Neogen's Theophylline ELISA (Enzyme-Linked ImmunoSorbent Assay) kit is a qualitative one-step kit designed for use as a screening test for the detection of drugs and/or their metabolites in equine or canine matrices. (neogen.com)
After the candidate compound is mixed with the target, the interaction between the compound and the target can be quantitatively determined by enzyme-linked immunoassay, fluorescence chromogenic assay, nuclear magnetic resonance, and other methods, thus becoming the basis for compound screening. (bocsci.com)
Both the chromogenic antibiotic permeability assay and fluorescent biosensor assay can be adapted to liposomes for higher throughput investigations (microplate assay). (jacobs-university.de)
Pharmaceutical microbiology includes the detection and assay of pharmaceutical and chemical products resulting from microbial activity. (pharmacyfreak.com)

High purity dyed, soluble Azo-CM-Cellulose for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis. (megazyme.com)
The purpose of drug screening is to identify and optimize potential drugs before selecting candidates to enter clinical trials, which may involve screening large pools of chemicals for specific biological activities in high-throughput screening assays. (bocsci.com)
We also perform more traditional, intra bacterial permeability assays, using chromogenic antibiotics whose color changes upon enzymatic degradation. (jacobs-university.de)
The use of CLS405 in assays was validated by MBL activity measurements and inhibitor screening that resulted in the identification of N-hydroxythiazoles as new inhibitor scaffolds for MBLs. (ox.ac.uk)

The crystallinity and the limited accessibility to the ester linkages make PET unsusceptible to enzymatic hydrolysis, but a number of ester-cleaving enzymes have been reported active on the polyester [ 1 ]. (biomedcentral.com)
The initial hydrolysis step of PET is facilitated by an extracellular enzyme homologous to cutinases, but with an extraordinary capability to degrade PET, hence classified as a PETase (EC 3.1.1.101). (biomedcentral.com)
Hydrolyzed Fumonisin B2 (HFB2) is a hydrolysis product of fumonisins (HF), which retains biological activity. (medchemexpress.com)
LTA 4 H EH activity catalyzes the hydrolysis of leukotriene A 4 (LTA 4 ) to the neutrophil chemoattractant leukotriene B 4 (LTB 4 ), while AP activity hydrolyzes the tripeptide proline-glycine-proline (PGP) to clear neutrophils in the lungs. (gmu.edu)
The product is marked with 100,000 enzyme activities, and the actual enzyme activity is 60,000, resulting in incomplete hydrolysis and indirectly increasing the cost of enzymes. (starbiozyme.com)
No enzymatic hydrolysis experience and enzyme compounding technology increase labor cost and R&D cycle, which indirectly affects the production schedule. (starbiozyme.com)
β-galactosidase , also called lactase , beta-gal or β-gal , is a glycoside hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides through the breaking of a glycosidic bond . (wikidoc.org)

PCI binds to immobilized urokinase and is then revealed by a monoclonal antibody coupled to the enzyme: peroxidase. (cryopep.com)
TMBZ is a chromogenic reagent utilized for peroxidase detection. (dojindo.com)
An Improved Staining Procedure for the Detection of the Peroxidase Activity of Cytochrome p-450 on Sodium Dodecyl Sulfate Polyacrylamide Gels. (dojindo.com)
The subject ANS analogs are characterized in that they react with MBTH or analogs thereof in the presence of an oxidizing agent, a peroxidase and oxygen to produce a chromogenic, specifically a blue, reaction product that exhibits reduced drift. (allindianpatents.com)
Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx) activities and uric acid (UA) levels were measured in plasma and saliva. (bvsalud.org)
To counteract the oxidative stress mammalian cells have antioxidant system which includes enzymatic activities such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx), and non enzymatic antioxidants including vitamins A, E, C, melatonin, uric acid (UA) and glutathione 6,10 . (bvsalud.org)

Extracellular enzyme profiling of M. chalcea revealed a general wide activity including: polysaccharide hydrolases, proteases, lipases, phosphatases and amino-peptidases. (scialert.net)
The goal of this study is to study the effect of various ratios of sludge, rice straw and M. chalcea inoculum on accelerating the straw decomposition in soil and to investigate the extracellular enzyme profile of M. chalcea that may contribute to accelerating the decomposition process. (scialert.net)

the corresponding nitrohalogenophenoxazinones were all characterised and evaluated for the detection of nitroreductase activity in a range of clinically important microorganisms. (sunderland.ac.uk)
A Double Sandwich Monoclonal Enzyme Immunoassay for Detection of Hepatitis B Surface Antigen. (dojindo.com)
The cellular level of drug screening is a drug screening model that is closer to the physiological condition, the model is proposed to design drug target cells, and the application of cell culture technology to obtain the required cells, these cells interact with candidate compounds, by screening and biochemical levels similar detection technology was developed for the determination of compound action ability, filtered by the compound. (bocsci.com)
For the first time, Western blot detection of LOS at the lower femtomole level was accomplished by both chromogenic and chemiluminescent detection. (go.jp)
The application of pharmaceutical microbiology is essential in the detection, assessment, and utilization of these microbial activities and products. (pharmacyfreak.com)

We show that PETases can be functionally displayed on the bacterial cell surface, enabling screening of enzyme activity on PET microparticles - both while anchored to the cell and following solubilization of the enzymes. (biomedcentral.com)
Thousands of chemical compounds with effective in vitro inhibition of essential bacterial targets have been identified by pharma companies. (jacobs-university.de)
Strikingly, almost none of them show reasonable antimicrobial activity when used in vivo , most especially in Gram-negative bacterial pathogens. (jacobs-university.de)

Plasmin is a serine protease with broad specificity which, in addition to cleavage of fibrin, is capable of activation and/or degradation of compounds of the coagulation, kinin generation and complement systems. (cellsystems.eu)
Enzymes power for plastics degradation. (toulouse-biotechnology-institute.fr)

Lithium chloride in the base medium and supplementary antimicrobial compounds ceftazidime, polymyxin, nalidixic acid, and cycloheximide provide the medium's selectivity. (thermalindo.com)
L-Dihydroorotic acid can reversibly hydrolyze to yield the acyclic L-ureidosuccinic acid by dihydrowhey enzyme. (medchemexpress.com)
2,6-Dihydroxybenzoic acid is a secondary metabolite of salicylic acid which has been hydrolyzed by liver enzymes during phase I metabolism. (medchemexpress.com)
A zinc containing enzyme of the hydrolase class that catalyzes the removal of the N-terminal amino acid from most L-peptides, particularly those with N-terminal leucine residues but not those with N-terminal lysine or arginine residues. (lookformedical.com)
An octameric enzyme belonging to the superfamily of amino acid dehydrogenases. (lookformedical.com)
A branched-chain essential amino acid that has stimulant activity. (lookformedical.com)
An enzyme that catalyzes the first step in the biosynthetic pathway to LEUCINE , forming isopropyl malate from acetyl-CoA and alpha-ketoisovaleric acid. (lookformedical.com)
These biosensors are engineered antibiotic targets or antibiotic degrading enzymes containing a fluorescent amino-acid in their active site. (jacobs-university.de)
Garcinia Cambodia extract standardized to contain 60% hydroxycitric acid (HCA) - from the point of view of fat loss, the most desirable aspect of HCA is that it functions as a competing inhibitor for ATP citrate lyase enzyme, and, as a result, inhibits the synthesis of fat. (fitnmuscles.com)
Green coffee bean extract standardized to contain 50% chromogenic acid (CGA) - limits the range of the fluctuations of the glucose level by inhibiting its absorption from the intestine, resulting in limited secretion and prolipogenic effect of insulin. (fitnmuscles.com)

Low antigenic and PCI activity values have been determined in patients with disseminated intravascular coagulation (DIC). (cryopep.com)

With that, we provide evidence that the well-defined perforations produced by protoplast feeders are caused by extracellular carbohydrate-active enzymes and made a first step towards establishing the molecular basis of a fascinating, yet poorly understood microbial feeding strategy. (megazyme.com)
O-Mucin-degrading carbohydrate-active enzymes and their possible implication in inflammatory bowel diseases. (toulouse-biotechnology-institute.fr)

Combining both the chromogenic and phospholipase enzyme reactions makes it possible to differentiate Listeria monocytogenes (blue colonies surrounded by an opaque halo) from other Listeria spp. (thermalindo.com)

A PYRIDOXAL PHOSPHATE containing enzyme that catalyzes the reversible transamination of branched-chain AMINO ACIDS to 2-oxoglutarate. (lookformedical.com)
This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. (cancerindex.org)

The high incidence of pneumococcal diseases is compounded by a rising trend of antimicrobial resistance among pneumococcal strains. (frontiersin.org)

Although few previous studies have focused on response surface optimization of lignocellulosic enzymes in fermentation media (Cacchio et al . (scialert.net)
2. The use of amylase in the production of alcohol and brewing industry can make the enzymatic activity evenly distributed during fermentation, improve the saccharification, alcohol yield and yeast growth. (starbiozyme.com)
Enzymes produced by microbial cells, both intracellular and extracellular, play a crucial role in the success of pharmaceutical fermentation processes. (pharmacyfreak.com)

Results: The GH5_5 catalytic domain from Orciraptor showed pronounced activity on soluble cellulose derivatives and mixed-linkage glucans, with reaction optima comparable to known GH5_5 representatives. (megazyme.com)

Helical inclusion complexes of amylose with aromatic compounds: crystallographic evidence for new V-type allomorphs. (toulouse-biotechnology-institute.fr)

This enzyme splits the (1→4) bonds from the non-reducing end of a chain releasing beta-maltosyl units, but cannot cleave the (1→6) bonds. (hmdb.ca)

Previously published studies focused on blocking EH activity to inhibit the LTB 4 formation with small molecule inhibitors. (gmu.edu)
Efforts to develop metallo-β-lactamase (MBL) inhibitors require suitable screening platforms to allow the rapid determination of β-lactamase activity and efficient inhibition. (ox.ac.uk)
The findings of this study provide both a useful tool compound for further inhibitor identification, and novel scaffolds for the design of improved MBL inhibitors with potential as antibiotics against resistant strains of bacteria. (ox.ac.uk)
Finally, the high proteolytic activity in COVID-19 lungs suggests considering protease inhibitors as a treatment option. (bvsalud.org)

belongs to the class of organic compounds known as pyridoxines. (ecmdb.ca)
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. (lookformedical.com)

however, as evidenced by genome sequence analysis, the organism lacks common enzymes required for the de novo synthesis of precursors of PGN, which rationalizes its MurNAc auxotrophy. (karger.com)

This review argues that the oxocarbenium ion intermediate reaction mechanism is more rational than the nucleophilic displacement reaction mechanism, as the action mode of glycosidases and related enzymes. (go.jp)

Among them AAT Bioquest's Buccutite™ crosslinking technology offer the most efficient method to conjugate antibodies with macromolecules such as enzyme tags. (stratech.co.uk)

Seven medicinal plants from Chiapas, Mexico, used by Native Americans were analyzed, aiming to improve the understanding of their medicinal properties through the evaluation of various biological activities, i.e., bactericidal, antioxidant, α-glucosidase inhibition, and toxicity, to provide a scientific basis for the management of infectious and hyperglycemic diseases in the Mexican southeast. (bvsalud.org)

Leukotriene A 4 hydrolase (LTA 4 H) is a bi-functional enzyme that promotes a pro-inflammatory epoxy hydrolase (EH) pathway and an anti-inflammatory aminopeptidase (AP) pathway. (gmu.edu)

At the end of the study period (6 weeks), blood pressure, blood glycosylated hemoglobin (HbA 1c ), serum creatinine, blood urea nitrogen (BUN), kidney tissue levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxide (GPx), aldose reductase (AR) activities and malondialdehyde (MDA) concentration were measured. (biomedcentral.com)
Many actinomycetes can degrade cellulose and solubilize the lignin structure as their primary metabolic activity at high nitrogen levels compared to white rot fungi (Eriksson et al . (scialert.net)
Nitrogen-fixing microorganisms provide nitrogen for crop growth, and enzymes aid plant growth, seed germination, and fruiting. (pharmacyfreak.com)

Ozone administration in conjunction with insulin in DM rats reduces oxidative stress markers and improves renal antioxidant enzyme activity which highlights its potential uses in the regimen for treatment of diabetic patients. (biomedcentral.com)

they are lignin-modifying enzymes. (wikipedia.org)
2003). However, cellulose crystallinity and lignin - which offers cellulose physical protection against cellulolytic enzymes-limit the decomposition of polysaccharides present in lignocellulose residues for biotechnological application (Lynd et al . (scialert.net)

Minimum Inhibitory Concentration (MIC) was employed to determine the antibacterial activity against Staphylococcus aureus, Enterobacter faecalis, Escherichia coli, Enterobacter aerogenes, Enterobacter cloacae, Klebsiella pneumoniae, and Pseudomonas aeuroginosa. (bvsalud.org)

Dose-dependent increase in glucose uptake activity (GUA) was observed in both cell lines. (wiley.com)

Currently, the permeability of the chromogenic β-lactam nitrocefin is being investigated in bacteria expressing the β-lactamase AmpC. (jacobs-university.de)

3. The alpha amylase enzyme activity can be maintained up to 90% for 6 months when stored at room temperature. (starbiozyme.com)

Resveratrol, which is abundant in grape skins and berries, may improve metabolic adaptation by stimulating enzymes involved in fat metabolism. (mid-day.com)
oritavancin will decrease the level or effect of carbamazepine by affecting hepatic/intestinal enzyme CYP3A4 metabolism. (medscape.com)
oritavancin will decrease the level or effect of disopyramide by affecting hepatic/intestinal enzyme CYP3A4 metabolism. (medscape.com)
oritavancin will increase the level or effect of fosphenytoin by affecting hepatic enzyme CYP2C9/10 metabolism. (medscape.com)

This enzyme activity will result in a halo of precipitation surrounding the target colonies. (thermalindo.com)

Crystalline cellulose was not digested by the enzyme, which suggests a typical endocellulase activity. (megazyme.com)

6 key control point inspections, escorting the whole process of quality, using extraction, membrane separation, vacuum freeze-drying, spray drying and enzyme activity protection technologies to produce products that fully meet the requirements of global customers. (starbiozyme.com)

To assess the importance of this carbohydrate-active enzyme in the feeding act of Orciraptor , we recombinantly produced its catalytic domain and studied the enzymatic activity, cellular localisation and function. (megazyme.com)

Lack of enzyme protection technology and quality control, unstable enzyme activity, and differences in product specifications for each batch, resulting in product quality fluctuations. (starbiozyme.com)

Coleus forskholi extract standardized to contain 10% forskolin - forskolin's basic mechanism is to activate the adenylyl cyclase enzyme, to increase the cyclical level of AMP (camp) and to activate related functions. (fitnmuscles.com)
Here we show that complement triggers contraction of TH1 responses by inducing intrinsic expression of the vitamin D (VitD) receptor and the VitD-activating enzyme CYP27B1, permitting T cells to both activate and respond to VitD. (bvsalud.org)

Many adult humans lack the lactase enzyme, which has the same function of beta-gal, so they are not able to properly digest dairy products. (wikidoc.org)

Further evaluation of the identified N-hydroxythiazoles against a panel of clinically relevant MBLs showed that they possess inhibitory activities in the mid- to low-micromolar range. (ox.ac.uk)

1 production line of ultra concentrate liquid enzyme. (starbiozyme.com)

Notanarin has comparable activity to factor Xa in cleaving fusion proteins. (venomsupplies.com)

After temperature changes, the combined effects of low lipid diet and low temperature conditions resulted in higher pepsin activity, while trypsin, chymotrypsin, and lipase activities were generally higher at high lipid content. (frontiersin.org)
This occurs in tissue cell cytosol, with high activity in the duodenum, liver , and kidney. (lookformedical.com)
It refers to the process of selecting compounds with high activity for a specific target from a large number of compounds or new compounds by standardized experimental means. (bocsci.com)
The enzymes will lose activity at high temperature or under strong sunlight. (starbiozyme.com)

This technique has been widely used in applied sciences such as optimizing enzyme production and assessment of drug interactions (Kalil et al . (scialert.net)

L-tyrosine - acts as a precursor for adrenaline and noradrenaline securing their level growth and the mental and physical activity thus helping to increase caloric expenditure. (fitnmuscles.com)

Chemicals and Drugs8

Phenomena and Processes1

Technology, Industry, Agriculture1

Information Science1

Substrate16

Substrates5

Assay5

Assays4

Hydrolysis7

Peroxidase6

Extracellular enzyme2

Detection5

Bacterial3

Degradation2

Acid10

Disseminated intravascul1

Carbohydrate-active enzymes2

Phospholipase1

Catalyzes2

Antimicrobial1

Fermentation3

Derivatives1

Aromatic1

Cleave1

Inhibitors4

Organic2

Synthesis1

Reaction1

Conjugate1

Inhibition1

Hydrolase1

Nitrogen3

Antioxidant1

Lignin2

Escherichia1

Glucose1

Bacteria1

Alpha-amylase1

Metabolism4

Colonies1

Cellulose1

Extraction1

Enzymatic activity1

Fluctuations1

Activate2

Lack1

Evaluation1

Concentrate1

Proteins1

High4

Assessment1

Growth1