Actin of skeletal. Medical search. Frequent questions

In budding yeast, the actin-binding protein Bud6 cooperates with formins Bni1 and Bnr1 to catalyze the assembly of actin filaments. (nih.gov)
These archaeal profilins also retard the spontaneous nucleation of actin filaments, an effect that is reduced in the presence of phospholipids. (rcsb.org)
The name actin-accumulation myopathy derives from characteristic accumulations in muscle cells of filaments composed of a protein called actin . (medlineplus.gov)
ATP is a molecule that supplies energy for cells' activities, and is important in the formation of thin filaments from individual actin molecules . (medlineplus.gov)
Pre-formed actin filaments (PAFs) are supplied as a lyophilized powder. (cytoskeleton.com)
These stringently quality controlled filaments provide highly reliable and reproducible results in assays that require actin filament substrates. (cytoskeleton.com)
Here we show that a minimal reconstituted system consisting of actin filaments, crosslinking molecules and molecular-motor filaments exhibits a generic mechanism of structure formation, characterized by a broad distribution of cluster sizes. (nature.com)
contra-ction - shortening of the muscles cross bridges - the head of a myosin molecule that projects from a myosin filament in muscle and in the sliding filament of muscle contra-ction is held to attach tempor-arily to an adjacent actin filament and draw it into the A band of a sarcomere between the myosin filaments. (cheatography.com)
muscle - body tissue made of long cells that contract when stimulated and produce motion myofibril - contra-ctile unit composed of myosin and actin myosin - fibrous protein that forms (together with actin) the contra-ctile filaments of muscle cells and is also involved in motion in other types of cells. (cheatography.com)
Titin activates myosin filaments in skeletal muscle by switching from an extensible spring to a mechanical rectifier. (unifi.it)
Chimp and human muscles fundamentally work the same, they say, with filaments of actin sliding past thick myosin bands to produce contractions. (discovermagazine.com)
Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. (thermofisher.com)
Thickening of the actin filaments at the cell edge provided the bending force to extrude the virus particles. (cdc.gov)

Here we show that Asgard archaea encode functional profilins and thereby establish that this archaeal superphylum has a regulated actin cytoskeleton, one of the hallmarks of the eukaryotic cell 5 . (rcsb.org)
Structure formation and constant reorganization of the actin cytoskeleton are key requirements for the function of living cells. (nature.com)
Joanny, J-F. & Prost, J. Active gels as a description of the actin-myosin cytoskeleton. (nature.com)
Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. (thermofisher.com)
Dystrophin-glycoprotein complex bridges the inner cytoskeleton (F-actin) and the basal lamina. (medscape.com)

Actin, alpha skeletal muscle is a protein that in humans is encoded by the ACTA1 gene. (wikipedia.org)
Description: A sandwich quantitative ELISA assay kit for detection of Human Actin Alpha 1, Skeletal Muscle (ACTa1) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids. (myelisakit.com)
Description: Quantitative sandwich ELISA for measuring Human Actin, alpha skeletal muscle (ACTA1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. (myelisakit.com)
Description: A competitive ELISA for quantitative measurement of Canine Actin, Alpha skeletal muscle(ACTA1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (therabio.org)
NM is caused by mutations in at least nine genes: Nebulin ( NEB ), α-actin ( ACTA1 ), α-tropomyosin ( TPM3 ), β-tropomyosin ( TPM2 ), troponin T ( TNNT1 ), cofilin-2 ( CFL2 ), Kelch repeat and BTB (POZ) domain-containing 13 ( KBTBD13 ), and Kelch-like family members 40 and 41 ( KLHL40 and KLHL41 ). (biomedcentral.com)
Actin-accumulation myopathy is caused by a mutation in the ACTA1 gene. (medlineplus.gov)
ACTA1 gene mutations that cause actin-accumulation myopathy may affect the way the skeletal α-actin protein binds to ATP. (medlineplus.gov)
In some people with actin-accumulation myopathy, no ACTA1 gene mutations have been identified. (medlineplus.gov)

Actins are highly conserved proteins that are involved in cell motility, structure and integrity. (wikipedia.org)
SRF may bring a number of other proteins to the promoter of skeletal actin, such as androgen receptor, and thereby contribute to induction of skeletal actin gene expression by androgenic (often termed "anabolic") steroids. (wikipedia.org)
Loki profilin-1, Loki profilin-2 and Odin profilin adopt the typical profilin fold and are able to interact with rabbit actin-an interaction that involves proteins from species that diverged more than 1.2 billion years ago 6 . (rcsb.org)
Actin proteins are important for cell movement and the tensing of muscle fibers (muscle contraction). (medlineplus.gov)
AKF99 is also an excellent substrate for detecting actin binding proteins, see Fig. 2. (cytoskeleton.com)
We also did selective epitope mapping using BLAST and showed similarities and homology between spike, nucleoprotein, and many other SARS-CoV-2 proteins with the human tissue antigens mitochondria M2, F-actin and TPO. (frontiersin.org)
To examine gender-dimorphic regulation of skeletal muscle proteins between healthy control and STZ-induced diabetic rats of both genders, we performed differential proteome analysis using two-dimensional electrophoresis combined with mass spectrometry. (karger.com)
Proteomic analysis of skeletal muscle identified a total of 21 proteins showing gender-dimorphic differential expression patterns between healthy controls and diabetic rats. (karger.com)
Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. (thermofisher.com)
Tropomyosin proteins regulate the tensing of muscle fibers (muscle contraction) by controlling the binding of two muscle proteins, myosin and actin. (medlineplus.gov)
Histopathologic changes were evaluated in medial gastrocnemius muscle samples using antibodies to cellular (actin, desmin, dystrophin, fast myosin) and extracellular (laminin, albumin, complement C3) proteins and markers for neutrophils (W3/13) and macrophages (ED1). (cdc.gov)

Actin-accumulation myopathy is a disorder that primarily affects skeletal muscles, which are muscles that the body uses for movement. (medlineplus.gov)
The severe muscle weakness that occurs in actin-accumulation myopathy also affects the muscles used for breathing. (medlineplus.gov)
This gene provides instructions for making a protein called skeletal alpha (α)-actin, which is a member of the actin protein family found in skeletal muscles. (medlineplus.gov)
The voluntary Skeletal Muscles are long, slender, multinucleate disks with obvious striations have T-tubules utilizing actin and troponin. (bartleby.com)
The force of the myosin motor sets cooperativity in thin filament activation of skeletal muscles. (unifi.it)
Where we differ, however, is in the type of fibers that together make up our skeletal muscles. (discovermagazine.com)
Limb-girdle muscular dystrophy refers to a group of genetic disorders that cause progressive weakness and wasting of the skeletal muscles, predominantly around the shoulders and hips. (medscape.com)
Slow muscle α-tropomyosin is found in skeletal muscles, which are the muscles used for movement. (medlineplus.gov)
Type I fibers are the primary component of skeletal muscles that are resistant to fatigue. (medlineplus.gov)
The only ways for you to express an idea are with the muscles of your larynx, mouth and tongue (spoken words), with the muscles of your fingers (written words or 'talking with your hands') or with the skeletal muscles (body language, dancing, running, building or fighting, to name a few). (howstuffworks.com)
Skeletal muscles attach to the skeleton and come in pairs -- one muscle to move the bone in one direction and another to move it back the other way. (howstuffworks.com)
In skeletal muscles, acetylcholine is released by nerve terminals at the motor end plate-the point of synaptic communication between motor neurons and muscle fibers. (jove.com)
Unlike skeletal muscles, smooth muscles present in the walls of internal organs are innervated by the autonomic nervous system and undergo involuntary contractions. (jove.com)
Prevention of histopathologic changes from 30 repeated stretches of active rat skeletal muscles by long inter-stretch rest times. (cdc.gov)

Actin alpha 1 which is expressed in skeletal muscle is one of six different actin isoforms which have been identified. (wikipedia.org)
Nebulin is a giant (600 to 900 kDa), thin-filament, actin-binding protein, and the gene comprises a total of 183 exons, of which at least 17 are alternatively spliced, producing hundreds of different NEB isoforms [ 20 ]. (biomedcentral.com)
Alpha, beta and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. (thermofisher.com)
Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates. (thermofisher.com)
Whether the differential expression of actin isoforms in human diseases is functionally relevant is not yet fully established, although studies on human actin mutations, actin null mice, and the N-terminal end of alpha-smooth muscle actin support this possibility. (unige.ch)

Nebulin is a giant (600 to 900 kDa) filamentous protein constituting part of the skeletal muscle thin filament. (biomedcentral.com)
The Bud6 binding site on actin overlaps with that of the formin FH2 domain and is also incompatible with inter-subunit contacts in F-actin, suggesting that Bud6 interacts only transiently with actin monomers during filament nucleation. (nih.gov)
Dysfunctional actin-ATP binding may result in abnormal thin filament formation and impair muscle contraction, leading to muscle weakness and the other signs and symptoms of actin-accumulation myopathy. (medlineplus.gov)
Bornemann A, Petersen MB, Schmalbruch H. Fatal congenital myopathy with actin filament deposits. (medlineplus.gov)
They have been shown to move cargoes such as vesicles along actin filament tracks. (cytoskeleton.com)

The immunogen used for this product shares 77% homology with Gamma actin/actin cytoplasmic 2. (abcam.com)

These mutations change single amino acids in slow muscle α-tropomyosin and are thought to impair the protein's ability to interact with myosin and actin within type I skeletal muscle fibers, disrupting muscle contraction. (medlineplus.gov)

Around 90% of the primary structure of nebulin is composed of approximately 35-residue α-helical domains, which form super repeats that bind actin with high affinity. (biomedcentral.com)
Myosin motors that cannot bind actin leave their folded OFF state on activation of skeletal muscle. (unifi.it)

Newly developed antibodies against alpha-skeletal and alpha-cardiac actins have allowed the detection of subpopulations of alpha-skeletal positive cardiomyocytes in adult, hypertrophic, and failing heart. (unige.ch)

One key transcription factor that activates skeletal actin gene expression is Serum Response Factor ("SRF"), a protein that binds to specific sites on the promoter DNA of the actin gene. (wikipedia.org)
The nucleation-enhancing activity of Bud6 requires both a "core" domain that binds to the formin and a "flank" domain that binds monomeric actin. (nih.gov)
one binds in a groove at the barbed end of the actin monomer in a manner closely resembling the helix of WH2 domains, a motif found in many actin nucleation factors. (nih.gov)

We demonstrate for the first time the existence of direct tropomyosin-nebulin interactions in vitro , and show that nebulin interactions with actin and tropomyosin are altered by disease-causing mutations in nebulin and tropomyosin. (biomedcentral.com)
The specific effects of these TPM3 gene mutations are unclear, but researchers suggest they may interfere with normal actin-myosin binding, impairing muscle contraction and resulting in the muscle weakness that occurs in cap myopathy. (medlineplus.gov)

We performed F-actin and tropomyosin-binding experiments for the nebulin super repeats, using co-sedimentation and GST (glutathione-S-transferase) pull-down assays. (biomedcentral.com)
WT nebulin was shown to interact with actin and tropomyosin. (biomedcentral.com)
Super repeats containing the deletion p.Val3924_Asn3929del showed similar affinity for actin and tropomyosin as that seen with WT super repeats. (biomedcentral.com)
Slow muscle α-tropomyosin helps regulate muscle contraction in type I skeletal muscle fibers. (medlineplus.gov)

Skeletal actin mRNA increases in the human heart during ontogenic development and is the major isoform of control and failing adult hearts. (jci.org)
In this report, we investigated the isoactin mRNA composition in ventricles from 21 control patients (4 fetal, 5 juvenile, 12 adult) and from 15 patients undergoing cardiac transplantation (5 idiopathic dilated cardiomyopathies, 5 ischemic myopathies with myocardial infarcts, 5 diverse etiologies) by two different and complementary techniques: RNA dot blot analysis with specific cDNA probes, and primer extensions with an oligonucleotide common to alpha-cardiac and alpha-skeletal actins. (jci.org)
In the case of dot blot analysis, quantification of each isoform was performed by using as standards RNA transcripts obtained from cloned human alpha-actin sequences, and the total amount of sarcomeric actin mRNA was evaluated as a function of total poly(A+)RNA. (jci.org)

Expression of the two sarcomeric actins, alpha-skeletal and alpha-cardiac, is regulated in the rodent heart in response to developmental, hormonal, and hemodynamic stimuli. (jci.org)
These observations demonstrate that cardiac development in man, in contrast to rodent heart, is characterized by an up-regulation of the skeletal actin gene, the expression of which does not change in hypertrophied and failing hearts, and suggest that the actin and myosin heavy chain families are independently regulated in human heart. (jci.org)
Does not cross-react with adult cardiac, smooth, or skeletal muscle actin. (abcam.com)
2. Delineate between the 3 types of muscle (skeletal, cardiac, and smooth)? (fsu.edu)

Actin, alpha 1 has been shown to interact with TMSB4X, MIB2 and PRKCE. (wikipedia.org)

PAFs have been prepared from rabbit skeletal muscle actin protein that is greater than 99% pure (Cat. (cytoskeleton.com)
Confocal immunofluorescent analysis of COS-7 cells using β-Actin (13E5) Rabbit mAb (Alexa Fluor ® 647 Conjugate) (blue pseudocolor). (cellsignal.com)
Flow cytometric analysis of Hela cells using β-Actin (13E5) Rabbit mAb (Alexa Fluor ® 647 Conjugate) (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control (Alexa Fluor ® 647 Conjugate) #2985 (dashed line). (cellsignal.com)

Alpha actins are a major constituent of the contractile apparatus. (wikipedia.org)
Skeletal alpha actin expression is induced by stimuli and conditions known to cause muscle formation. (wikipedia.org)
In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. (thermofisher.com)
Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. (thermofisher.com)
A voluntary skeletal muscle contraction begins in the brain as a conscious effort from the frontal lobe to the primary motor cortex, before activating, an alpha motor neuron located in the anterior horn of the spinal cord. (jove.com)
p-Tau-Ser356 and PP2A-alpha (MAB1653) levels were normalized to the total amount of β-actin (NB600-501) protein in the brain tissue of diabetic (D) rats injected with insulin receptor inhibitor IOMe and treated with NSO (Nigella sativa oil). (novusbio.com)
alpha-Smooth muscle actin has proven to be a reliable marker for identifying (a) vascular smooth muscle cells during vascular development and vascular diseases, and (b) myofibroblasts during wound healing, fibrocontractive diseases, and stromal reaction to epithelial tumours. (unige.ch)
The hallmark of a differentiated myofibroblast relies on the acquisition of an organized contractile apparatus characterized by alpha-smooth muscle actin-expressing stress fibres. (unige.ch)
More and more data suggest that alpha-smooth muscle actin plays a direct role in myofibroblast contractile activity through its N-terminal domain AcEEED. (unige.ch)

The results obtained by administering Blebbistatin propose a titin-actin interaction that depends on XB-binding or active XB-based force production. (frontiersin.org)
Matching Mechanics and Energetics of Muscle Contraction Suggests Unconventional Chemomechanical Coupling during the Actin-Myosin Interaction. (unifi.it)
Identification of icsA, a plasmid locus of Shigella flexneri that governs bacterial intra- and intercellular spread through interaction with F-actin. (microbiologyresearch.org)

In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. (thermofisher.com)
Skeletal muscle is made up of two types of muscle fibers: type I (slow twitch fibers) and type II (fast twitch fibers). (medlineplus.gov)
A myosin-based mechanism for stretch activation and its possible role revealed by varying phosphate concentration in fast and slow mouse skeletal muscle fibers. (umass.edu)
After 2 days, histopathologic changes were present only in samples from the S40 protocol including desmin negative fibers [162 (32)] which stained for albumin, complement C3, laminin, actin and fast myosin. (cdc.gov)

Skeletal actin itself, when expressed, causes expression of several other "myogenic genes", which are essential to muscle formation. (wikipedia.org)
Because Asgard archaea are also predicted to encode potential eukaryotic-like genes involved in membrane-trafficking and endocytosis 3,4 , imaging is now necessary to elucidate whether these organisms are capable of generating eukaryotic-like membrane dynamics that are regulated by actin, such as are observed in eukaryotic cell movement, podosomes and endocytosis. (rcsb.org)

The following product was used in this experiment: smooth muscle actin Monoclonal Antibody (1E9A11) from Thermo Fisher Scientific, catalog # 67735-1-IG. (thermofisher.com)

They function as actin-based molecular motors. (nih.gov)
My research goal is to understand how structural and functional alterations at the molecular and single fiber levels affect whole skeletal muscle contraction and whole body function, primarily to find potential countermeasures to the loss of skeletal muscle performance under a variety of conditions, including aging, obesity, fatigue and illness (influenza). (umass.edu)
An important aspect of my research is the ability to measure the fundamental molecular determinants of contractile function, such as myosin-actin cross-bridge kinetics. (umass.edu)
By identifying the specific molecular mechanisms that lead to alterations in whole muscle function, my findings will impact clinical care by providing novel information about the treatment of conditions that reduce skeletal muscle performance. (umass.edu)
Straight CR, Ringham OR, Bartley JM, Keilich SR, Kuchel GA, Haynes L, Miller MS. Influenza infection has fiber type-specific effects on cellular and molecular skeletal muscle function in aged mice. (umass.edu)
Moderate-intensity resistance exercise alters skeletal muscle molecular and cellular structure and function in inactive, older adults with knee osteoarthritis. (umass.edu)

Skeletal abnormalities are frequent complications of patients with a congenital myopathy. (medscape.com)

Mutational analysis verifies the importance of these Bud6-actin contacts for nucleation-enhancing activity. (nih.gov)

Kane, R. E. Interconversion of structural and contractile actin gels by insertion of myosin during assembly. (nature.com)

Exposure of cells to soluble factors that damage cell membranes results in the release of actin into the extracellular spaces. (microbiologyresearch.org)
These results indicate that extracellular actin enhances haemolysin production by E. coli and may have implications in the pathogenesis of E. coli infections. (microbiologyresearch.org)

Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. (thermofisher.com)
Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. (thermofisher.com)
Multiple alternatively spliced variants of smooth muscle actin have been identified. (thermofisher.com)
It can stretch in a limited way, like smooth muscle, and contract with the force of a skeletal muscle. (howstuffworks.com)

Homozygotes for a targeted null mutation exhibit retarded growth, impaired fertility, skeletal dysplasias, and peri- and postnatal lethality. (jax.org)

Here, we describe the structure of the Bud6 flank domain in complex with actin. (nih.gov)
Skeletal muscle has a complex structure that is essential to how it contracts. (howstuffworks.com)

Structural transitions of F-actin upon ATP hydrolysis at near-atomic resolution revealed by cryo-EM. (expasy.org)

Modulation of actin isoform expression is a well-established feature of developmental phenomena. (unige.ch)

Myofiber-specific ablation of IRE1α dampens Notch signaling and canonical NF-κB pathway in skeletal muscle of adult mice. (elifesciences.org)

Residual force depression (rFD) following active muscle shortening and residual force enhancement (rFE) following active muscle stretch ( Abbott and Aubert, 1952 ) are fundamentally accepted mechanical properties of skeletal muscle ( Rassier, 2017 ). (frontiersin.org)

EN)do-mysium- connective tissue that covers the muscle fiber. (cheatography.com)
This prevents transmission of electrical signals between the motor neuron and muscle fiber and impairs skeletal muscle contraction. (jove.com)

These studies were conducted to determine if actin influences growth and haemolytic activity of E. coli. (microbiologyresearch.org)
Growth of E. coli in the presence of actin resulted in culture supernatant haemolytic activity that was 2.4-, 2.7- and 3.3-fold greater than that of E. coli grown in medium containing BSA, non-supplemented medium, or medium containing heat-denatured actin, respectively. (microbiologyresearch.org)
Actin induced a 2.9-fold increase in alkaline phosphatase activity in E. coli CC118 with a TnphoA insertion in the hlyB determinant of the recombinant haemolysin piasmid pWAM04. (microbiologyresearch.org)

The enhanced haemolytic activity occurred only when actin was present during the growth phase and there was no effect when actin was added to culture supernatants containing haemolysin. (microbiologyresearch.org)

Skeletal muscle, the most abundant tissue of the body, has remarkable regenerative capacity mainly due to its resident muscle stem cells, also known as satellite cells. (elifesciences.org)

Anatomy33

Organisms12

Diseases1

Chemicals and Drugs113

Analytical, Diagnostic and Therapeutic Techniques and Equipment19

Phenomena and Processes37

Disciplines and Occupations2

Technology, Industry, Agriculture3

Information Science5

Filaments13

Cytoskeleton5

ACTA18

Proteins11

Muscles14

Isoforms5

Filament5

Cytoplasmic1

Myosin and actin1

Bind actin2

Antibodies1

Binds3

Mutations2

Tropomyosin4

MRNA3

Cardiac4

Interact1

Rabbit3

Alpha9

Interaction3

Fibers4

Genes2

Antibody1

Molecular6

Congenital1

Nucleation1

Contractile1

Extracellular2

Smooth4

Postnatal1

Complex2

Structural1

Modulation1

Adult1

20171

Muscle fiber2

Coli3

Supernatants1

Abundant1