• Acridine dyes are prepared via the condensation of 1,3-diaminobenzene with suitable benzaldehydes. (wikipedia.org)
  • Morphological features of the roots were assessed using a root scanner, and then attempts were made to stain the roots in four types of dyes: 0.01% methylene blue, 0.01% acridine orange, 0.01% malachite green, and 0.01% carbol fuchsin. (scirp.org)
  • Traditional Giemsa-stained thick blood films were compared with 2 fluorescence microscopy techniques, acridine orange (AO) staining of thin blood films and the quantitative buffy coat (QBC) method, for the microscopical diagnosis of malaria. (ox.ac.uk)
  • The cell viability and apoptosis were determined by CCK-8 assay and acridine orange/ethidium bromide (AO/EB) fluorescence staining assay. (greenmedinfo.com)
  • 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, acridine orange, and monodansylcadaverine staining and flow cytometry were performed. (hindawi.com)
  • Apoptosis was checked by acridine orange/ethidium bromine staining assay and by execution of Western blotting analysis. (ijpsonline.com)
  • Acridine orange/ethidium bromine staining assay revealed that piperine induced apoptosis in MCF-7 cells which was further investigated by Western blotting. (ijpsonline.com)
  • Acridine orange is used in epifluorescence microscopy and flow cytometry. (wikipedia.org)
  • When acridine orange is used with flow cytometry, the differential stain is used to measure DNA denaturation and the cellular content of DNA versus RNA in individual cells, or detect DNA damage in infertile sperm cells. (wikipedia.org)
  • Acridine orange is able to withstand low pH environments, allowing the fluorescent dye to penetrate acidic organelles such as lysosomes and phagolysosomes that are membrane-bound organelles essential for acid hydrolysis or for producing products of phagocytosis of apoptotic cells. (wikipedia.org)
  • Apoptotic cells which nucleolus shrinked and rounded could be coloured orange by fluorescent colouration. (researchgate.net)
  • Autophagy induction was confirmed via acridine orange staining. (iasp-pain.org)
  • Autophagy was detected using acridine orange staining and real-time PCR for Lc3 and Beclin1 genes expression. (ijpsonline.com)
  • Acridine orange has been widely accepted and used in many different areas, such as epifluorescence microscopy, and the assessment of sperm chromatin quality. (wikipedia.org)
  • A direct counting method using acridine orange staining and epifluorescence microscopy was attempted to assess the possible magnitude of the error associated with indirect counting. (bioone.org)
  • Acridine orange and fluorescein have a maximum excitation at 502nm and 525 nm (green). (wikipedia.org)
  • When acridine orange associates with RNA, the fluorescent dye experiences a maximum excitation shift from 525 nm (green) to 460 nm (blue). (wikipedia.org)
  • When acridine orange binds to DNA, the dye exhibits a maximum excitation at 502 nm producing a maximum emission of 525 nm. (wikipedia.org)
  • When bound to RNA, acridine orange displays a maximum emission value of 650 nm and a maximum excitation value of 460 nm. (wikipedia.org)
  • The maximum excitation and emission value that occur when acridine orange is bound to RNA are the result of electrostatic interactions and the intercalation between the acridine molecule and nucleic acid-base pairs present within RNA and DNA. (wikipedia.org)
  • When acridine orange is excited by blue light, the fluorescent dye can differentially stain human cells green and prokaryotic cells orange (600 nm), allowing for rapid detection with a fluorescent microscope. (wikipedia.org)
  • Past and present studies comparing acridine orange staining with blind subcultures for the detection of positive blood cultures showed that the acridine orange is a simple, inexpensive, rapid staining procedure that appears to be more sensitive than the Gram stain for detecting microorganisms in cerebrospinal fluid and other clinical and non-clinical materials. (wikipedia.org)
  • Acridine orange staining has to be performed at an acidic pH to obtain the differential staining, which allows bacterial cells to stain orange and tissue components to stain yellow or green. (wikipedia.org)
  • Acridine orange is recommended for the use of fluorescent microscopic detection of microorganisms in smears prepared from clinical and non-clinical materials. (wikipedia.org)
  • Acridine orange is useful in the rapid screening of ordinarily sterile specimens. (wikipedia.org)
  • Rapid diagnosis of malaria using acridine orange (AO) staining and a light microscope with a halogen lamp and interference filter was deployed in some malaria-endemic countries. (biomedcentral.com)
  • Acridine has antimicrobial factors useful in drug-resistant bacteria and isolating bacteria in various environments. (wikipedia.org)
  • Acridine orange in the mid-twentieth century was used to examine the microbial content found in soil and direct counts of aquatic bacteria. (wikipedia.org)
  • Additionally, the method of acridine orange direct count (AODC) proved useful in the enumeration of bacteria found within landfills. (wikipedia.org)
  • The use of acridine orange in clinical applications has become widely accepted, mainly focusing on highlighting bacteria in blood cultures. (wikipedia.org)
  • Acridine orange is an organic compound that serves as a nucleic acid-selective fluorescent dye with cationic properties useful for cell cycle determination. (wikipedia.org)
  • The ability to penetrate the cell membranes of acidic organelles and cationic properties of acridine orange allows the dye to differentiate between various types of cells (i.e., bacterial cells and white blood cells). (wikipedia.org)
  • Direct epifluorescent filter technique (DEFT) using acridine orange is a method known for examining the microbial content within food and water. (wikipedia.org)
  • When the pH of the environment is 3.5, acridine orange becomes excited by blue light (460 nm). (wikipedia.org)
  • Eth)high cells display a reduced incorporation of the cardiolipin-specific dye nonyl-acridine orange (NAO), showing a structural defect of the cardiolipin-containing inner mitochondrial membrane. (nih.gov)
  • Anticancer effects of the HDAC inhibitor, 3β,6β‑dihydroxyurs‑12‑en‑27‑oic acid, in MCF‑7 breast cancer cells via the inhibition of Akt/mTOR pathways. (iasp-pain.org)
  • In vitro, lysosomal destabilization, likely a result of particle uptake, was evident by acridine orange staining. (cdc.gov)
  • Acridine orange is able to withstand low pH environments, allowing the fluorescent dye to penetrate acidic organelles such as lysosomes and phagolysosomes that are membrane-bound organelles essential for acid hydrolysis or for producing products of phagocytosis of apoptotic cells. (wikipedia.org)
  • In addition, frankly apoptotic cells showed lower cardiolipin levels (by nonyl-acridine orange staining). (nih.gov)
  • The efficiency of energy transfer between nonyl-acridine orange and CMXRos was slightly lower in camptothecin-treated nonapoptotic cells and reduced to zero in frankly apoptotic cells. (nih.gov)
  • The tube is centrifuged and stained with a fluorescent stain, acridine orange. (healthy.net)
  • Unlike methods used in conventional blood analyzers, undiluted blood samples are stained with nucleic acid stain acridine orange. (caltech.edu)
  • With respect to apoptosis, the presence of mitochondrial membrane potential can be probed with rhodamine 123 while the structure and integrity of mitochondria can be assessed using 10-N-nonyl-acridine orange. (enzolifesciences.com)
  • The so-called potential-independent dyes like MitoView™ Green , MitoTracker® Green, and Nonyl Acridine Orange are much more hydrophobic than potential-responsive dyes like MitoView™ 633 , Rhodamine 123 , and JC-1 . (biotium.com)
  • For example, Nonyl Acridine Orange is reported to bind cardiolipin, a lipid that is enriched in mitochondrial membranes. (biotium.com)
  • The cell viability and apoptosis were determined by CCK-8 assay and acridine orange/ethidium bromide (AO/EB) fluorescence staining assay. (greenmedinfo.com)
  • After infection of cells using LV-DSCR1 + , acridine orange and ethidium bromide staining was performed to investigation of apoptosis and autophagy. (techscience.com)
  • Fragmented bright orange nucleuses and vacuoles were observed due to the cell apoptosis and autophagy after acridine orange and ethidium bromide staining. (techscience.com)
  • Acridine Orange hydrochloride solution has been used to study autophagic cell death. (umass.edu)
  • Fluorescent dyes with aromatic amino or guanidine groups, such as propidium iodide (PI), diaminophenylindole (DAPI), acridine orange (AO), and Hoechst dyes, interact with nucleotides to emit fluorescence. (dojindo.com)
  • 11. Comparison of two flow cytometric assays for cellular RNA--acridine orange and propidium iodide. (nih.gov)
  • Acridine orange is an organic compound that serves as a nucleic acid-selective fluorescent dye with cationic properties useful for cell cycle determination. (wikipedia.org)
  • The maximum excitation and emission value that occur when acridine orange is bound to RNA are the result of electrostatic interactions and the intercalation between the acridine molecule and nucleic acid-base pairs present within RNA and DNA. (wikipedia.org)
  • Past and present studies comparing acridine orange staining with blind subcultures for the detection of positive blood cultures showed that the acridine orange is a simple, inexpensive, rapid staining procedure that appears to be more sensitive than the Gram stain for detecting microorganisms in cerebrospinal fluid and other clinical and non-clinical materials. (wikipedia.org)
  • Comparative study of subculture, Gram staining and acridine orange staining for early detection of positive blood cultures. (bmj.com)
  • Acridine Orange is also used to analyze autophagy. (umass.edu)
  • The ability to penetrate the cell membranes of acidic organelles and cationic properties of acridine orange allows the dye to differentiate between various types of cells (i.e., bacterial cells and white blood cells). (wikipedia.org)
  • Acridine orange staining has to be performed at an acidic pH to obtain the differential staining, which allows bacterial cells to stain orange and tissue components to stain yellow or green. (wikipedia.org)
  • At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in the acidic organelle giving a bright red fluorescence. (umass.edu)
  • SNP elevated phrase amounts of g62, ATG7, LC3-II and Beclin-1, as regular autophagic indicators and increased acidic autophagolysosomal vacuoles, discovered by acridine tangerine discoloration. (exposed-skin-care.net)
  • Samples may also be analyzed with Acridine Orange or Hoechst stain to detect lysosomal organelle structure or nuclear morphology respectively. (immunochemistry.com)
  • Additionally, the method of acridine orange direct count (AODC) proved useful in the enumeration of bacteria found within landfills. (wikipedia.org)
  • Direct epifluorescent filter technique (DEFT) using acridine orange is a method known for examining the microbial content within food and water. (wikipedia.org)
  • Thin smears obtained from 548 Thai and Burmese patients were reviewed by an acridine orange staining method, and many mixed infections with two to four species, including P. malariae and P. ovale, were detected. (nih.gov)
  • Acridine orange in the mid-twentieth century was used to examine the microbial content found in soil and direct counts of aquatic bacteria. (wikipedia.org)
  • Total acridine orange direct cell counts of the flushed and nonflushed soils decreased over the 15-week testing period, but after 5 weeks, the flushed soils maintained higher cell counts than the nonflushed soils. (nih.gov)
  • were the first to report the effectiveness of acridine orange (AO) treatment as an adjuvant therapy in soft tissue sarcomas in various different studies ( 2 - 8 ). (iiarjournals.org)
  • Split red/green fluorescence, DIC and overlay images captured by Paige Bothwell, featuring SK Hep cells, a human hepatocellular carcinoma cell line, stained with acridine orange. (niu.edu)
  • Acridine orange is cell-permeable, which allows the dye to interact with DNA by intercalation, or RNA via electrostatic attractions. (wikipedia.org)
  • Although a few studies have shown that adjuvant therapy with acridine orange (AO) is effective for local control of primary STS, there have been no reports examining its effectiveness for local recurrence. (iiarjournals.org)
  • Moreover, acridine orange staining revealed no increased cell death upon scylla/charybdis coexpression. (sdbonline.org)
  • The differential staining capability of acridine orange provides quick scanning of specimen smears at lower magnifications of 400x compared to Gram stains that operate at 1000x magnification. (wikipedia.org)