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DyesApoptosisDAPIEthidiumSmearsGiemsaTransmission electron mZincCell viabilityMetachromatic dyeCellsDiagnosisNucleic acidsStainsChromatinLysosomal46005SpermSmearFACScanBacteriaMoleculeMoleculesMutagenStainingPeripheral bloodDerivativesNeutralConcentrationAqueousAcidicHigh purityBindsDimethylaminobenzaldehydeGreenGramProbeEvaluation
Dyes3
Apoptosis4
  • Cell viability was measured by MTS assay and apoptosis was assessed by Annexin V/PI and acridine orange staining. (nih.gov)
  • Acridine orange staining was used to visualize apoptosis in A172 (A) and U87 (B). Cells were treated with 0, 10 or 100 ng/ml rhTRAIL WT and 0, 25 or 50 μM DMC. (nih.gov)
  • Vital staining with the dye, acridine orange, has been previously used to analyze patterns of apoptosis in both vertebrate and invertebrate embryos (3,4). (alpfmedical.info)
  • During apoptosis, cells fragment into a number of apoptotic bodies that are chromatin-rich and are intensely stained by acridine orange. (alpfmedical.info)
DAPI3
Ethidium3
Smears5
  • Acridine orange stain is particularly useful in the rapid screening of normally sterile specimens, and its recommended for the use of fluorescent microscopic detection of microorganisms in direct smears prepared from clinical and non-clinical materials. (wikipedia.org)
  • The use of acridine orange stained buffy coat smears was assessed as a rapid screening test for bacteremia in adults. (hindawi.com)
  • Direct staining of blood smears with acridine orange and enumeration using fluorescent microscopy allowed quantitation of Haemophilus influenzae in blood at densities from 10(5) to 10(8) cfu/ml. (curehunter.com)
  • Fluorescent (acridine orange) microscopical examination of capillary centrifuged blood (quantitative buffy coat [QBC] analysis) and Giemsa stained thick blood smears (GTS) were compared for diagnosis of malaria in blood specimens from adults living in malaria transmission areas of the States of Bolivar and Amazonas in southeastern and south Venezuela, respectively. (nih.gov)
  • Comparison of the automicrobic system, acridine orange-stained smears, and gram-stained smears in detecting bacteriuria. (asm.org)
Giemsa3
  • Giemsa staining, acridine orange (AO) staining and semi-nested multiplex PCR. (curehunter.com)
  • The present study compares the value of the Acridine Orange technique and the Giemsa staining technique for the detection of Dipetalonema perstans in blood films. (journals.co.za)
  • The study revealed that the Acridine technique was at least as sensitive as the older Giemsa technique, but proved quicker and less tiring to use. (journals.co.za)
Transmission electron m2
  • Autophagy were assessed by acridine orange staining, MDC staining and Transmission electron microscopy. (nih.gov)
  • Autophagic cell death induced by SAHA was confirmed by acridine orange staining and transmission electron microscopy (TEM) in TAMR/MCF-7 cells. (medsci.org)
Zinc1
  • We offer a highly purified form of acridine orange while most of the other commercially available grades are either of low purity or in zinc chloride complex form. (polysciences.com)
Cell viability1
Metachromatic dye2
Cells13
  • Thus, acridine orange can be used to visualize primary lysosomes and phagolysosomes that may include products of phagocytosis of apoptotic cells. (wikipedia.org)
  • At a low pH (3.5), when acridine orange is excited by blue light, it can differentially stain human cells green while staining prokaryotes bright orange for detection with a fluorescence microscope. (wikipedia.org)
  • Acridine orange is a versatile fluorescence dye used to stain acidic vacuoles (lysosomes, endosomes, and autophagosomes), RNA, and DNA in living cells. (wikipedia.org)
  • After 24h, cells were stained using acridine orange dye. (nih.gov)
  • Moreover, it helps in staining human cells with and prokaryotes with green and bright orange color, respectively. (alphachemikaindia.com)
  • This is done during the excitation of Acridine Orange under blue light at pH level of 3.5 while detecting the cells with the help of fluorescence microscope. (alphachemikaindia.com)
  • The acridine orange fluorescence stain reliably demonstrated malignant cells based on the differential fluorescence - a cytochemical criterion. (bvsalud.org)
  • However, the acridine orange fluorescence stain did not differentiate effectively between malignant cells and rapidly proliferating cells, as the technique is based on the nucleic acid content. (bvsalud.org)
  • Liapunova E.A., Zelenin A.V., The effect of acridine orange on the early steps of protein biosynthesis in tissue culture cells, Voprosy meditsinskoi khimii, 1966, vol: 12(2), 218-220. (msk.ru)
  • Cells were then stained with acridine orange 1: Control group. (nih.gov)
  • We used the acridine orange test (AOT) and the terminal deoxynucleotidyl transferase -mediated dUDP nick-end labeling ( TUNEL ) assay to assess the DNA status of sperm cells preserved with different lyophilization media. (bvsalud.org)
  • VectaCell Acridine Orange and VectCell Rhodamine 123 reagents offer convenience and ease of use for visualizing different cellular components in live cells. (vectorlabs.com)
  • The two micrographs (right) show live mononuclear cells stained with acridine orange in green, and dead cells stained with PI in red. (nexcelom.com)
Diagnosis2
Nucleic acids3
Stains4
Chromatin1
  • Acridine orange has been widely accepted and used in many different areas, such as epifluorescence microscopy, the assessment of sperm chromatin quality. (wikipedia.org)
Lysosomal1
460051
Sperm3
Smear3
FACScan2
  • We do routinely use acridine orange using FACScan. (purdue.edu)
  • creswelk at georgetown.edu ] Sent: Friday, June 04, 2004 1:17 PM To: cyto-inbox Subject: Acridine Orange Does anyone routinely run acridine orange through their FACScan/FACSort? (purdue.edu)
Bacteria3
Molecule1
Molecules1
Mutagen1
Staining10
Peripheral blood1
Derivatives2
  • We have synthesized acridine orange derivatives which comprise an imidazole-2-carbaldehyde function for coordination to the Re(CO)(3)](+) or (99)mTc(CO)(3)](+) core. (uzh.ch)
  • Derivatives of acridine are successful as antibacterial agents, finding their principal use as local antiseptics. (thefreedictionary.com)
Neutral1
  • Non-toxic levels of neutral red and acridine orange were suitable tracers: they were visible in the stylet and gut after feeding for 24 h, and may also attract aphids to feed. (mdpi.com)
Concentration3
Aqueous1
Acidic2
High purity1
Binds1
Dimethylaminobenzaldehyde1
Green4
  • Bright orange organisms are easily detected against a black to faint green background. (wikipedia.org)
  • red indicates high interlinkedness of a term with other terms, orange, yellow and green decreasing interlinkedness. (uzh.ch)
  • Acridine yellow, also known as acridine yellow G, acridine yellow H107, basic yellow K, and 3,6-diamino-2,7-dimethylacridine, is a yellow dye with strong bluish-green fluorescence. (wikipedia.org)
  • polychromatic erythrocytes (PCE) stained red/orange, nonchromatic erythrocytes (NCE) are unstained (dull green), and micronuclei stain bright yellow. (europa.eu)
Gram2
  • Later on, Lauer, Reller and Mirret performed a similar study, compared acridine orange with the Gram stain for detecting the microorganisms in cerebrospinal fluid and other clinical materials. (wikipedia.org)
  • The sensitivity of Gram stain (GS) was 98.26% while Acridine Orange (AO) stain proved to be more sensitive (100%) with a Positive and Negative Predictive Value of 100% each. (deepdyve.com)
Probe2
Evaluation1