• A flow cytometer is similar to a microscope, except that, instead of producing an image of the cell, flow cytometry offers high-throughput, automated quantification of specified optical parameters on a cell-by-cell basis. (wikipedia.org)
  • In order to ensure high fidelity performance, we have implemented a high-speed microscope for in-process quality control, and image-based confluence measurements for automated dilution ratio calculation. (frontiersin.org)
  • The application of both dynamic and static imaging methods over the past two decades has revealed a fundamental aspect of immune function, namely, the key role for the spatial organization of innate and adaptive immune cells within a tissue in mediating effective host defense or producing pathology. (bmj.com)
  • In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. (wikipedia.org)
  • In this review, our aim is to present an overview of the utilization of multiplex imaging in immuno-oncology studies and inform novice researchers about the fundamental principles at each stage of the imaging and analysis process. (bmj.com)
  • citation needed] A flow cytometer has five main components: a flow cell, a measuring system, a detector, an amplification system, and a computer for analysis of the signals. (wikipedia.org)
  • This paper presents methods for using superecliptic pHluorin, a pH-sensitive GFP variant, as a tool for quantification of endocytic events in living cells using quantitative fluorescence microscopy and flow cytometry. (jove.com)
  • Flow cytometry (FC) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. (wikipedia.org)
  • In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. (wikipedia.org)
  • Modern flow cytometers are able to analyze many thousands of particles per second, in "real time" and, if configured as cell sorters, can actively separate and isolate particles with specified optical properties at similar rates. (wikipedia.org)
  • The sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is characteristic to the cells and their components. (wikipedia.org)
  • Other instruments using flow cytometry include cell sorters which physically separate and thereby purify cells of interest based on their optical properties. (wikipedia.org)
  • The flow cell has a liquid stream (sheath fluid), which carries and aligns the cells so that they pass single file through the light beam for sensing. (wikipedia.org)
  • From quantifying the expression of surface antigens, to determining the physiological changes in cells and everything in between, flow cytometry is as indispensable to a cell biologist as a knife is to a surgeon. (bitesizebio.com)
  • In most people's minds a flow cytometer can sort, view and count cells e.g. lymphocytes, thymocytes, cultured cells and even non-mammalian cells such as yeast or bacteria. (bitesizebio.com)
  • Allow the cells to equilibrate for two minutes before beginning imaging. (jove.com)
  • Finally, flow cytometry was used to show that the fluorescence from MitoSOX™ Red, a selective indicator of superoxide in mitochondria, was statistically similar in both control cells and cells incubated in DM-SWNTs. (biomedcentral.com)
  • At the 5th American Engineering Foundation Conference on Automated Cytology in Pensacola (Florida) in 1976 - eight years after the introduction of the first fluorescence-based flow cytometer (1968) - it was agreed to commonly use the name "flow cytometry", a term that quickly became popular. (wikipedia.org)
  • I have worked in flow cytometry for a number of years. (bitesizebio.com)
  • Uses for flow cytometry include: Cell counting Cell sorting Determining cell characteristics and function Detecting microorganisms Biomarker detection Protein engineering detection Diagnosis of health disorders such as blood cancers Measuring genome size A flow cytometry analyzer is an instrument that provides quantifiable data from a sample. (wikipedia.org)
  • So you've got your flow cytometry training booked and are one step closer to that precious data. (bitesizebio.com)
  • Spectral unmixing in flow cytometry is the key to great data from your full spectrum flow cytometry. (bitesizebio.com)
  • I am sure that you are rocking your data acquisition at this point, having perfected your understanding of panel setup, fluorophore usage, and using the flow cytometer of your choice. (bitesizebio.com)
  • Approximately three hours before imaging, measure the OD 600 of each overnight culture. (jove.com)
  • Mack Fulwyler was the inventor of the forerunner to today's flow cytometers - particularly the cell sorter. (wikipedia.org)
  • Soon after, flow cytometry instruments were developed, including the Cytofluorograph (1971) from Bio/Physics Systems Inc. (later: Ortho Diagnostics), the PAS 8000 (1973) from Partec, the first FACS (fluorescence-activated cell sorting) instrument from Becton Dickinson (1974), the ICP 22 (1975) from Partec/Phywe and the Epics from Coulter (1977/78). (wikipedia.org)
  • A flow cytometer is similar to a microscope, except that, instead of producing an image of the cell, flow cytometry offers high-throughput, automated quantification of specified optical parameters on a cell-by-cell basis. (wikipedia.org)
  • citation needed] A flow cytometer has five main components: a flow cell, a measuring system, a detector, an amplification system, and a computer for analysis of the signals. (wikipedia.org)
  • Flow cytometry is a pervasive tool to characterize just about anything in cell biology. (bitesizebio.com)