This book annotates what is known about the function of the 150 genes encoded by the genome of the baculovirus AcMNPV. (e-booksdirectory.com)
We study the early and late replication stages of the prototype baculovirus, Autographa californica nuclear polyhedrosis virus (AcMNPV) in cultured cells. (wisc.edu)
Previously, we have used the baculovirus, AcMNPV, and the baculovirus GP64 envelope glycoprotein as models for our studies. (btiscience.org)
CpBV promoters showed activity earlier than the polyhedrin promoter and the activity of some of these promoters was superior to that of the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ie-1 promoter in the baculovirus expression assays. (microbiologyresearch.org)
Autographa3
Genetic optimization of the Autographa californica nucleopolyhedrovirus (AcMNPV) genome yields recombinant virus in a single step and eliminates the need for separation of recombinant and parental virus by plaque purification. (fishersci.com)
Very late expression factor (VLF-1) of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is essential for high levels of expression of the very late genes p10 and polh, and evidence suggests VLF-1 may also be involved in viral DNA replication. (elsevierpure.com)
To understand how baculoviruses replicate and process their genomes and the gene products that are involved in these events, a series of mutant virus constructs were generated using the Autographa californica multinucleopolyhedrovirus (AcMNPV) genome propagated as a bacteria artificial chromosome (bacmid). (mediu.edu.my)
Genome1
In this study, investigations determined whether VLF-1 is essential for viral DNA replication by generating a vlf-1 knockout bacmid containing the AcMNPV genome through homologous recombination in Escherichia coli. (elsevierpure.com)
Genes3
Using a genetic complementation assay to identify additional genes which inhibit apoptosis during infection with a p35 mutant, we have isolated a gene from Orgyia pseudotsugata NPV (OpMNPV) that was able to functionally substitute for AcMNPV p35. (embl.de)
AcMNPV uses a complex regulatory scheme to ensure the proper timing and level of expression of essential genes. (wisc.edu)
We have characterized the promoters and enhancers required for proper transcription of early AcMNPV genes and continue to investigate the trans-acting factors involved. (wisc.edu)
We have developed AcMNPV-permissive cell lines to examine both viral envelope protein requirements, as well as cellular factors involved in entry and egress. (btiscience.org)
Nucleopolyhedroviruses (NPVs), a genus of the multiple NPV (AcMNPV) illness and proposed a role for TBP during late viral transcription (50). (kentlandsinitiative.org)
Cells2
found that a warmth shock protein 70 cognate of Sf9 cells is definitely transiently induced at 6 hpi during AcMNPV illness (42). (kentlandsinitiative.org)
The promoter of ORF3004 showed the highest level of activity in insect cells, exhibiting 24 % of the activity obtained with the AcMNPV polyhedrin promoter in Sf9 cells. (microbiologyresearch.org)
Virus2
Find out how LC-MS was used to look for problematic Sf9 and AcMNPV host cell proteins in an adeno-associated virus based product. (alphalyse.com)
The Birnbaum-Saunders distribution was found to be consistent with all three‐virus packaging levels, including nucleocapsids (NCs)/occluded derived virus (ODV), ODVs/polyhedra and polyhedra/cell for both Wild‐type and genetically modified AcMNPV. (iith.ac.in)
Cell1
AcMNPV also causes programmed cell death. (wisc.edu)
Permissive3
AcMNPV in permissive, semipermissive, and nonpermissive cell lines from Arthropoda. (cellosaurus.org)
Ao38, a new cell line from eggs of the black witch moth, Ascalapha odorata (Lepidoptera: Noctuidae), is permissive for AcMNPV infection and produces high levels of recombinant proteins. (cellosaurus.org)