• Like other Acholeplasma and Mycoplasma, A. laidlawii has been identified as a common contaminant of growth media for cell culture. (wikipedia.org)
  • Grygorieva, Maxim V. Trushin, Innokentii E. Vishnyakov, Anton V. Sabantsev, Sergei N. Borchsenius, and Olga A. Chernova, "Extracellular membrane vesicles secreted by mycoplasma Acholeplasma laidlawii PG8 are enriched in virulence proteins," Journal of Proteomics , 2014. (hindawi.com)
  • The octadecenoic acid growth-requirement for Acholeplasma ( Mycoplasma ) laidlawii a can be effectively replaced by cis -9,10-methylenehexadecanoic acid but not by m-9,10-hexadecenoic acid. (microbiologyresearch.org)
  • Five species of Mollicutes - Mycoplasma arginini, M. fermentans, M. hyorhinis, M. orale, and Acholeplasma laidlawii - account for ~95% of identified cell culture contamination ( 1 , 3 ). (bioprocessintl.com)
  • This allows for amplification of all Mycoplasma and Acholeplasma species usually encountered as contaminants in cell cultures. (dkfz.de)
  • A panel of 61 mollicutes, including representatives from the Mycoplasma , Acholeplasma , Mesoplasma , Spiroplasma and Ureaplasma genus, were selected to evaluate the sensitivity and specificity of these assays. (biomedcentral.com)
  • The porcine Mycoplasma and Acholeplasma strains used in this study are listed in Table 1 . (asm.org)
  • Mycoplasma pneumoniae, M. hominis, M. arthritidis, M. gallisepticum , and Acholeplasma laidlawii grew on Millipore (mixed cellulose ester) filters and Sepraphore III (cellulose polyacetate) membranes. (asm.org)
  • BLAST for two isolates revealed 99% similarity to Acholeplasma laidlawii from India, another two were 99% similar to MmmLC strain from Sweden, two showed 98% similarity to Mycoplasma sp. (scielo.org.za)
  • The MycoSEQ™ Mycoplasma Detection Assay, based on real-time PCR and Power SYBR® Green detection technology, is designed for the routine screening and detection of Mycoplasma and other closely related species such as Acholeplasma laidlawii and Spiroplasma citri. (thermofisher.com)
  • Intensive bioinformatics analysis was utilized to design amplification primers and reaction conditions to allow comprehensive detection of known Mycoplasma, Acholeplasma, and Spiroplasma species (Table 1), while avoiding detection of related bacterial species. (thermofisher.com)
  • This polyclonal antiserum was evaluated by double immunodiffusion test (DIT) and counter-immune electrophoresis (CIE) against Mycoplasma bovis, Acholeplasma laidlawii and Ureaplasma diversum. (scirp.org)
  • Acholeplasma species serve as natural hosts. (eol.org)
  • There are currently only one genus ( Plasmavirus ), and one species in this family: the type species Acholeplasma virus L2 . (eol.org)
  • The type species is Acholeplasma laidlawii . (thefreedictionary.com)
  • Acholeplasma laidlawii are small bacteria which lack a cell wall. (wikipedia.org)
  • U14905 Acholeplasma laidlawii PG8 ATCC 23206 16S ribosomal RNA gene, partial sequence. (atcc.org)
  • Grigorieva, Marina N. Davydova, Maxim V. Trushin, Olga A. Chernova, and Vladislav M. Chernov, "Adaptation of Mycoplasmas to Antimicrobial Agents: Acholeplasma laidlawii Extracellular Vesicles Mediate the Export of Ciprofloxacin and a Mutant Gene Related to the Antibiotic Target," The Scientific World Journal , vol. 2014, pp. 1-7, 2014. (hindawi.com)
  • A monotopic membrane protein, monoglucosyldiacylglyecerol synthase (MGS) from Acholeplasma laidlawii is known to induce intracellular vesicles when expressed in Escherichia coli . (diva-portal.org)
  • Transport properties of membrane vesicles from Acholeplasma laidlawii. (meta.org)
  • The glucose transport system of membrane vesicles isolated from Acholeplasma laidlawii is saturable, with a Km of 21.2 mum and V of 0.68 nmol min-1 (mg protein)-1. (meta.org)
  • A. laidlawii has a relatively small genome comprising 1.5Mbp. (wikipedia.org)
  • The A. laidlawii genome has been sequenced. (wikipedia.org)
  • Type strain of Acholeplasma laidlawii at BacDive - the Bacterial Diversity Metadatabase A. laidlawii genome sequence from NCBI. (wikipedia.org)
  • Acholeplasma laidlawii PG-8A, complete genome. (atcc.org)
  • CC Acholeplasma laidlawii PG-8A chromosome, complete genome. (univ-lyon1.fr)
  • Each batch of media was tested for the ability to support the growth of a standard strain of Acholeplasma laidlawaii obtained from the Department of Veterinary Public Health, College of Veterinary Sciences, Chaudhary Charan Singh Haryana Agricultural University, Hisar. (thefreedictionary.com)
  • The fatty acid composition of the membrane polar lipids of A. laidlawii a grown with cis -11,12-octadecenoic and cis -9,10-methylenehexadecanoic acids shows each acid to be incorporated to a similar extent. (microbiologyresearch.org)
  • A. Wieslander and O.P. Karlsson , Regulation of Bilayer and Non-Bilayer Forming Lipids in Acholeplasma laidlawii . (elsevier.com)
  • Among those, the foreign monotopic glycosyltransferase MGS from Acholeplasma laidlawii triggers such massive lipid synthesis when overexpressed. (diva-portal.org)
  • I en studie fra Canada hvor kyllingene fikk ulike antibiotika, blant annet narasin, heter det: «Our study clearly shows that broiler chickens act as a reservoir for commensal E. coli strains carrying large numbers of antibiotic resistance genes. (tidsskriftet.no)
  • A cell-free system from A. laidlawii a synthesized less cw-11,12- methyleneoctadecanoic and tetradecanoic acids but more octadecanoic acid when compared with the fatty acid composition of the intact organism. (microbiologyresearch.org)
  • Chernov V.M., O.A. Chernova, E.S. Medvedeva, A.A. Mouzykantov, A.A. Ponomareva, G.F. Shaymardanova, O.V. Gorshkov, Trushin M.V. Unadapted and adapted to starvation Acholeplasma laidlawii cells induce different responses of Oryza sativa, as determined by proteome analysis // Journal of Proteomics. (kpfu.ru)
  • Fourier transform infrared spectroscopy is applied to the study of intact and deproteinated plasma membranes of Acholeplasma laidlawii, enriched biosynthetically with perdeuteriopalmitoyl chains. (canada.ca)
  • X15508 Acholeplasma laidlawii gene for tRNA-Trp. (atcc.org)
  • The expression of the mellitin gene in A. laidlawii andM. (deepdyve.com)
  • The presence of A. laidlawii in broth powders is a serious problem in routine biopharmaceutical operations where filtration is used as a sterilisation procedure. (wikipedia.org)
  • Acholeplasma appears as a contaminant in tissue culture media that have been supplemented with animal sera. (thefreedictionary.com)
  • A subcommittee has chosen A. laidlawii grown to a small size as a model to demonstrate the greatest filter challenge. (bioprocessintl.com)
  • Acholeplasma laidlawii may contaminate bovine serum and also occurs in serum-free cell culture media products. (wikipedia.org)
  • A. laidlawii may flourish and survive for prolonged periods at refrigeration and ambient temperatures in serum-free cell culture media. (wikipedia.org)
  • After transformation of Acholeplasma laidlawii andMycoplasma hominiscells with recombinant plasmid DNAs by electroporation, cell growth was suppressed. (deepdyve.com)
  • It is a two-layer, highly asymmetric, 0.6-µm filter validated for 1010 A. laidlawii reduction or better. (bioprocessintl.com)