Increased GSIS after PPM1E silencing was associated with decreased oxidative stress, elevated cytosolic Ca 2+ levels and ATP to ADP ratio, increased hyperpo-larization of the inner mitochondrial membrane, and phosphorylation of CaMKII, AMPK, and acetyl-CoA car-boxylase. (lu.se)
The aim of this study was to examine the state of AMPK phosphorylation histologically in primary breast cancer in relation to clinical and pathological parameters. (biomedcentral.com)
Immunohistochemistry was performed using antibodies to phospho-AMPK (pAMPK), phospho-Acetyl Co-A Carboxylase (pACC) an established target for AMPK, HER2, ERα, and Ki67 on Tissue Micro-Array (TMA) slides of two cohorts of 117 and 237 primary breast cancers. (biomedcentral.com)
One direct downstream target of AMPK is acetyl-Coenzyme A carboxylase (ACC). (biomedcentral.com)
LW1564 also significantly decreased overall ATP levels by inhibiting mitochondrial electron transport chain (ETC) complex I and downregulated mammalian target of rapamycin (mTOR) signaling by increasing the AMP/ATP ratio, which increased AMP-activated protein kinase (AMPK) phosphorylation. (biomed.news)
AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. (arigobio.cn)
Moreover, CG stimulated the phosphorylation of AMP-activated protein kinase (AMPK), and the protective effect of CG on hepatocytes was partially reversed both by the inhibitor of AMPK signaling pathway and overexpression of AMPK-DN. (bvsalud.org)
Here we report a new level of regulation wherein LCFA-CoA esters per se allosterically activate AMP-activated protein kinase (AMPK) β1-containing isoforms to increase fatty acid oxidation through phosphorylation of acetyl-CoA carboxylase. (mcmaster.ca)
Activation of AMPK by LCFA-CoA esters requires the allosteric drug and metabolite site formed between the α-subunit kinase domain and the β-subunit. (mcmaster.ca)
Thus, LCFA-CoA metabolites act as direct endogenous AMPK β1-selective activators and promote LCFA oxidation. (mcmaster.ca)
Genetic deletion of the AMPK β1 subunit in mice ( referred to herein as β1-/- mice ) reduced macrophage AMPK activity, acetyl-CoA carboxylasephosphorylation, and mitochondrial content, resulting in reduced rates of fatty acid oxidation. (edu.au)
Results: We demonstrate that leptin stimulates AMPK activity and increases AMPK Thr172 and acetyl-CoA carboxylase-β Ser222 phosphorylation and fatty acid oxidation in lean myotubes but that in obese subjects leptin-dependent AMPK signaling and fatty acid oxidation are suppressed. (mcmaster.ca)
They were accompanied by increases in both the activity (88%) and mRNA (51%) of malonyl-CoA decarboxylase (MCD) in muscle but no changes in the phosphorylation of AMP kinase (AMPK, Thr172) or of acetyl-CoA carboxylase. (uthscsa.edu)
In addition, YSY promoted lipid metabolism by inducing adiponectin secretion and activation (phosphorylation) of 5' adenosine monophosphate-activated protein kinase (AMPK) and by suppressing the activation of acetyl-CoA carboxylase (ACC) and HMG-CoA reductase (HMGCR). (tmu.edu.tw)
AMPK also increases fatty acid oxidation by phosphorylating and inactivating acetyl-CoA carboxylase, along with the decreasing concentration of malonyl-CoA, an inhibitor of fatty acid transport into mitochondria [ 12 ]. (biomedcentral.com)
Activation of chemomigration-related cell signaling (extracellular signal-regulated kinase (ERK), 5' adenosine monophosphate-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC)) was evaluated by Western blot analysis. (unimi.it)
Moreover, U0126 and Compound C (both 10 μM) counteracted FAC-driven phosphorylation of ACC, an AMPK downstream protein. (unimi.it)
Oxidative phosphorylation5
We, therefore, suggest that LW1564 may be a potent therapeutic agent for a subset of cancers that rely on oxidative phosphorylation for ATP generation. (biomed.news)
He found that, even in the presence of ample oxygen, cancer cells prefer to metabolize glucose by glycolysis, a seeming paradox as glycolysis, when compared to oxidative phosphorylation, is a less efficient pathway for producing ATP (Warburg, 1956). (pharmaceuticalintelligence.com)
UCP1 is specific to the mitochondria of brown fat, and functions as a proton transporter, uncoupling oxidative phosphorylation from ATP synthesis and allowing the resulting energy to dissipate in the form of heat. (mrsupps.com)
Free circulating insulin activates insulin receptors on the surface of target cells eliciting a signaling cascade initiated by the activation of insulin receptor substrates (IRS 1-4) followed by phosphorylation of phosphoinositide 3-kinase (PI3K), which manages metabolic response including PDK1 and Akt stimulation by phosphorylation. (encyclopedia.pub)
Phosphorylation of TSC2 following insulin or IGF1 receptor-mediated activation on the MAP and PI3K kinase pathways market. (rockinhibitor.com)
Then, activation of cGMP-dependent protein kinase strengthens the phosphorylation of cardiac troponin C, the affinity of troponin C to Ca2+ decreases, and the activity of K+ channels on the muscle cell membrane also decreases, resulting in vasodilation. (mgm-biochem.com)
4] The ATP depletion is due to activation of Na+/K+ -ATPase, which, in turn, is a consequence of decreased phosphorylation by protein kinase C. Activation of the NMDA receptor is probably the cause of seizures in acute hyperammonemia. (medscape.com)
Enzyme8
The systematic name of this enzyme class is ATP:[acetyl-CoA carboxylase] phosphotransferase. (wikipedia.org)
The PathScan ® Phospho-Acetyl-CoA Carboxylase (Ser79) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of acetyl-CoA carboxylase (ACC) when phosphorylated at Ser79. (cellsignal.com)
Silencing acetyl-CoA carboxylase 1 (an enzyme producing malonyl-CoA) normalized malonyl-CoA levels and reactivated mTOR in FASN(KD) ECs. (ugent.be)
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. (polabo.com)
ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. (polabo.com)
The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. (polabo.com)
Pyruvate dehydrogenase is a multi-enzyme complex responsible for the generation of acetyl CoA from pyruvate for the Krebs cycle. (msdmanuals.com)
Pyruvate carboxylase is an enzyme important for gluconeogenesis from pyruvate and alanine generated in muscle. (msdmanuals.com)
Hormone-sensit3
It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. (arigobio.cn)
regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. (arigobio.cn)
NO can increase the phosphorylation of hormone-sensitive lipase and translocate it to neutral fat granules, thereby activating fat degradation. (mgm-biochem.com)
Coenzyme A carboxylase2
Phosphorylation state of acetyl-coenzyme A carboxylase. (wikipedia.org)
N terminal acetylation or CH3CO as epigenetic regulation of Anti-Acetyl Coenzyme A Carboxylase alpha (Ser80) by NATs.The Anti-Acetyl Coenzyme A Carboxylase alpha (Ser80) is a α- or alpha protein sometimes glycoprotein present in blood.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. (polabo.com)
Protein5
The relationship between the protein concentration of lysates from untreated and H 2 O 2 -treated Hep G2 cells and the absorbance at 450 nm using the PathScan ® Phospho-Acetyl-CoA Carboxylase (Ser79) Sandwich ELISA Kit is shown. (cellsignal.com)
Regulation of protein phosphorylation in β-cells has been extensively investigated, but less is known about protein dephosphorylation. (lu.se)
Thus, protein de-phosphorylation via PPM1E abrogates GSIS. (lu.se)
2001). Activation of Acetyl-CoA Carboxylase by a Glutamate and Magnesium-Sensitive Protein Phosphatase in the Islet b-Cell. (iribb.org)
Pathway1
While the salicylate group implicates in the anti-inflammatory and anti-cancer properties via targeting cyclin A2/CDK2, HMGB1 and NF-κB pathway [ 11 - 13 ], the acetyl group causes the inactivation of cyclooxygenases (COXs) through acetylation of serine residues [ 14 ]. (oncotarget.com)
Phosphorylation at Thr-172 and consequent activation occurs in response to metabolic stresses that deplete cellular energy levels and thus increase the AMP/ATP ratio [ 1 , 2 ]. (biomedcentral.com)
Antibody1
The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676 (left panel) or Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody #3661 (right panel) are shown in the bottom figure. (cellsignal.com)
NO produced by arginine can stimulate the phosphorylation of Therefore, by inhibiting the activity of acetyl COA carboxylase and activating the activity of malonyl COA decarboxylase, the content of malonyl COA can be reduced, and the genes related to fat and glycogen synthesis can be reduced. (mgm-biochem.com)
Oxidation3
When it is inactivated by phosphorylation, a decrease in malonyl-CoA occurs, thereby increasing the mitochondrial import and oxidation of long chain fatty acids (LCFAs), resulting in the generation of ATP [ 12 ]. (biomedcentral.com)
LCFA-CoA esters promote their own oxidation by acting as allosteric inhibitors of acetyl-CoA carboxylase, which reduces the production of malonyl-CoA and relieves inhibition of carnitine palmitoyl-transferase 1, thereby promoting LCFA-CoA transport into the mitochondria for β-oxidation2-6. (mcmaster.ca)
Ordinary white fat stores energy in the form of fat droplets, one droplet per adipocyte, which can be broken down by lipolysis into free fatty acids which are circulated into the blood stream and transported to the fuel-requiring tissue where they undergo beta-oxidation to acetyl-CoA which can be used to generate energy through the Krebs cycle. (mrsupps.com)
Increases3
Exercise training decreases the concentration of malonyl-CoA and increases the expression and activity of malonyl-CoA decarboxylase in human muscle. (uthscsa.edu)
In conclusion, the concentration of malonyl-CoA is diminished in muscle after physical training, most likely because of PGC-1alpha-mediated increases in MCD expression and activity. (uthscsa.edu)
Dive into the research topics of 'Exercise training decreases the concentration of malonyl-CoA and increases the expression and activity of malonyl-CoA decarboxylase in human muscle. (uthscsa.edu)
These include beta-hydroxybutyrate dehydrogenase and acetoacetyl coenzyme A (CoA) synthetase (see the image below). (medscape.com)
Catalyzes the conversion1
Pyruvate carboxylase catalyzes the conversion of pyruvate to oxaloacetate (OAA) in the Krebs cycle. (lifespan.io)
Insulin1
On the other hand, SB elicited insulin gene expression in rat islets through increasing H3K18bu occupation in its promoter, without stimulating CREB phosphorylation. (bvsalud.org)
Activity2
Those composition could be used as an booster of palm oil production which related to acetyl co-A carboxylase activity (ACC). (iribb.org)
Furthermore, stearoyl-CoA desaturase-1 (SCD1), a transcriptional target of SREBP1, mediates the ferroptosis-suppressing activity of SREBP1 by producing monounsaturated fatty acids. (biomed.news)
Stimulates1
Treatment of Hep G2 cells with H 2 O 2 stimulates phosphorylation of ACC at Ser79, detected by the PathScan ® Phospho-ACC (Ser79) Sandwich ELISA Kit #7986, but does not affect the levels of total ACC detected by PathScan ® Total ACC Sandwich ELISA Kit #7996. (cellsignal.com)
The study was designed to evaluate whether changes in malonyl-CoA and the enzymes that govern its concentration occur in human muscle as a result of physical training. (uthscsa.edu)
Significant decreases (25-30%) in the concentration of malonyl-CoA were observed after training, 24-36 h after the last bout of exercise. (uthscsa.edu)
Metformin1
Administration of metformin considerably inhibited ERK1/2 phosphorylation in obese rat endometrium compared with TBK1 drug non-metformin treated controls (Figure 4B). (rockinhibitor.com)