Acetobutylicum gene. Medical search. Frequent questions

Clostridium acetobutylicum, ATCC 824, is a commercially valuable bacterium sometimes called the "Weizmann Organism", after Jewish Russian-born biochemist Chaim Weizmann. (wikipedia.org)
ATCC reference organism 824 C.Acetobutylicum. (wikipedia.org)
The integrated model was able to successfully reproduce ABE fermentations of the WT C. acetobutylicum (ATCC 824), as well as its mutants, using data obtained from our own experiments and from literature. (illinois.edu)
To overcome this substrate utilization defect, a predicted glcG gene, encoding enzyme II of the D-glucose phosphoenolpyruvate-dependent phosphotransferase system (PTS), was first disrupted in the ABE-producing model strain Clostridium acetobutylicum ATCC 824, resulting in greatly improved D-xylose and L-arabinose consumption in the presence of D-glucose. (hw.ac.uk)
Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824. (nottingham.ac.uk)
Results: We describe here the construction and validation of a GSM model for C. acetobutylicum ATCC 824, iCac802. (psu.edu)

Clostridium acetobutylicum, which is also known as the 'Weizmann organism,' was … This is mainly because solvents and organic acids could be used for production of ﬁne chemicals such as butyl butyrate, butyl oleate, etc. (thecoffeeparlor.com)
Interestingly, while genes which commonly code for cellusomes, protein complexes which breakdown crystalline cellulose, are present the organism is unable to grow solely on cellulose substrates (7). (thecoffeeparlor.com)
Clostridium acetobutylicum is an organism historically used for. (thecoffeeparlor.com)
In the past I have worked on metabolic engineering of the same organism by disrupting genes to asses their impact on acid and solvent formation. (fairdomhub.org)

Archived 2012-03-18 at the Wayback Machine findarticles.com: Bacteria speeds drug to tumors - use of Clostridium acetobutylicum enzyme to activate cancer drug CB 1954. (wikipedia.org)
When biologists seed a fresh batch of sugary broth with C. acetobutylicum bacteria, the first thing those microbes do is harvest the sugar's energy and multiply-their simple method of reproduction. (icr.org)
In addition to being an important bacteria for industrial use, C. acetobutylicum is studied as model for endospore formation in bacteria. (thecoffeeparlor.com)
Identification and alignment of the promoter regions of the transcriptional start sites of glycolytic genes revealed strong similarities to the σ A consensus promoter sequences of Gram-positive bacteria. (microbiologyresearch.org)
In other research projects, he explores physiology and gene regulation in biotechnologically relevant bacteria like different Bacillus species, Gluconobacter oxydans, and solventogenic Clostridia, and furthermore the biosynthesis of hydrocarbons and natural gene transfer in high-GC Gram-positive bacteria like Micrococcus luteus. (tum.de)
Here we demonstrate the formation of an artificial consortium between two anaerobic bacteria, Clostridium acetobutylicum (Gram-positive) and Desulfovibrio vulgaris Hildenborough (Gram-negative, sulfate-reducing) in which physical interactions between the two partners induce emergent properties. (hal.science)
Some bacteria have the bioY gene clustering with bioB , which encodes biotin synthase, an enzyme that converts dethiobiotin to biotin. (tcdb.org)

Unlike yeast, which can digest only some sugars into alcohol and carbon dioxide, C. acetobutylicum and other Clostridia can digest whey, sugar, starch, cellulose and perhaps certain types of lignin, yielding n-butanol, propionic acid, ether, and glycerin. (wikipedia.org)
In this process, acetone, butanol and ethanol are produced in a mixed fermentation (typically in a ratio of 3:6:1) by the solventogenic Clostridium species Clostridium acetobutylicum , Clostridium beijerinckii , Clostridium saccharobutylicum or Clostridium saccharoperbutylacetonicum from sugar or starch feedstocks 7 . (nature.com)
Integrated, systems metabolic picture of acetone-butanol-ethanol fermentation by Clostridium acetobutylicum. (icr.org)
One canonical example of such processes is acetone-butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum, during which cells convert carbon sources to organic acids that are later reassimilated to produce solvents as a strategy for cellular survival. (illinois.edu)
History of Commercial Use and Products Subject to TSCA Jurisdiction C. acetobutylicum has a long history of safe use in the industrial production of acetone and butanol in fermentation systems using maize mash, molasses, or other feedstocks. (thecoffeeparlor.com)
George, and S.M. Williams and Wilkins, Baltimore, MD. Currently, there is a resurgence of interest in Clostridium acetobutylicum, the biocatalyst of the historical Weizmann process, to produce n-butanol for use both as a bulk chemical and as a renewable alternative transportation fuel. (thecoffeeparlor.com)
In this study, production of butanol directly from hemicellulose was achieved simply through overexpression of an indigenous xylanase in Clostridium acetobutylicum. (thecoffeeparlor.com)
Disruption of the particular genes in proline biosynthesis pathways clarified the essential role of the anabolic ProB-ProA-ProI system over the osmoadaptive ProH-ProA-ProJ system for cellular protection in response to butanol exposure. (biomedcentral.com)
Molecular modifications to increase gene dosage for proline biosynthesis as well as for glutamate acquisition enhanced butanol tolerance of B. subtilis 168 up to 1.8% (vol/vol) under the conditions tested. (biomedcentral.com)
The Gram-positive anaerobic bacterium Clostridium acetobutylicum, known for its excellent capability of producing ABE (acetone, butanol, and ethanol) solvent, is limited in using lignocellulose because of inefficient pentose consumption when fermenting sugar mixtures. (hw.ac.uk)
In particular, Clostridium acetobutylicum has been used to produce butanol on an industrial scale through acetone-butanol-ethanol (ABE) fermentation. (psu.edu)
pH control has been essential for butanol production with Clostridium acetobutylicum . (springeropen.com)

One of the crucial enzymes - a fatty acyl-CoA reductase - came from Clostridium acetobutylicum. (wikipedia.org)
Molecular biology has allowed a detailed understanding of genes and enzymes, required for solventogenesis. (igem.org)
This physical interaction induces changes in expression of two genes encoding enzymes at the pyruvate crossroads, with concomitant changes in the distribution of metabolic fluxes, and allows a substantial increase in hydrogen production without requiring genetic engineering. (hal.science)

University of Illinois Engineering News wrote, 'Researchers from the University of Illinois at Urbana-Champaign have, for the first time, uncovered the complex interdependence and orchestration of metabolic reactions, gene regulation, and environmental cues of clostridial metabolism. (icr.org)
This is when gene regulation comes into play. (icr.org)
For example, in describing one aspect of building their digital version of the bacteria's gene regulation module, the researchers wrote, 'Here, the concentrations of the four key molecules (Spo0A, Spo0A∼P, σF, and σK) were adopted as the main model variables, and their kinetics were described using differential equations. (icr.org)
Microbial metabolism involves complex, system-level processes implemented via the orchestration of metabolic reactions, gene regulation, and environmental cues. (illinois.edu)
Here, we present a system-level computational framework for ABE fermentation that combines metabolic reactions, gene regulation, and environmental cues. (illinois.edu)
Regulation of pyruvate carboxylase isozyme ( PYC1 , PYC2 ) gene expression in Saccharomyces cerevisiae during fermentative and nonfermentative growth. (microbiologyresearch.org)
A systems biology approach to investigate the effect of pH-induced gene regulation on solvent production by Clostridium acetobutylicum in continuous culture. (kimosys.org)
By whole transcriptome analyses, we characterized glucosedependent and CcpA-dependent gene regulation in Clostridium difficile. (studyres.com)
About 18% of all C. difficile genes are regulated by glucose, for which 50% depend on CcpA for regulation. (studyres.com)
These two genes play opposite roles in regulation of colistin production. (hindawi.com)

The CcpA regulon comprises genes involved in sugar uptake, fermentation and amino acids metabolism, confirming the role of CcpA as a link between carbon and nitrogen pathways. (studyres.com)

Both 13 C-MFA and gene deletion data in the ABE fermentation pathway were used to test the predicted flux ranges allowed by the model. (psu.edu)

This will facilitate the functional analyses of genes as well as metabolic engineering for the production of biofuels and bioproducts from biomass. (biomedcentral.com)
To develop commercial processes for the production of bulk chemicals via a metabolic engineering approach it is necessary to better characterize both the primary metabolism and … C. acetobutylicum requires anaerobic conditions in order to grow in its vegetative state. (thecoffeeparlor.com)
This genome-scale model (iHN637) consisting of 637 genes, 785 reactions, and 698 metabolites captures all the major central metabolic and biosynthetic pathways, in particular pathways involved in carbon fixation and energy conservation. (biomedcentral.com)
Analysis of the iHN637 metabolic model revealed that flavin based electron bifurcation played a key role in energy conservation during autotrophic growth and helped identify genes for some of the critical steps in this mechanism. (biomedcentral.com)
The COBRA approach relies on a genome-scale metabolic network reconstruction, which enumerates the metabolic transformations and the genes encoding them in a mathematical format. (biomedcentral.com)
The pathways were constructed based on the presence or absence of key metabolic genes in D. hafniense DCB-2. (screeninglibraries.com)
Taken together, our data demonstrated that starch instead of glucose can promote colistin production probably by affecting the expression of colistin biosynthesis-related genes, as well as reducing the repression of glucose to a secondary metabolic product. (hindawi.com)

A bacterial strain producing two antimicrobial peptides was isolated from a rhizosphere soil sample and identified as Bacillus subtilis based on both phenotypic and 16S rRNA gene sequence phylogenetic analysis. (springeropen.com)

All other glycolytic genes except pps, glk and pgi were transcribed as monocistronic mRNAs under all tested conditions. (microbiologyresearch.org)
Growth in the presence of glucose gave rise to abundant expression of most glycolytic genes, with the level of gapA transcript being the highest. (microbiologyresearch.org)
Glucose depletion led to a rapid repression of most glycolytic genes and a corresponding two- to fivefold increased expression of the gluconeogenic genes pps, pck and malE , which are induced by pyruvate, lactate, acetate and/or other organic acids. (microbiologyresearch.org)

Bacterial cells from different species can combine into unique hybrid cells by fusing their cell walls and membranes and sharing cellular contents, including proteins and ribonucleic acid (RNA), the molecules which regulate gene expression and control cell metabolism. (azolifesciences.com)

Two buy Staurosporine copies of the gene (Dhaf_1324 and Dhaf_2460) coding for tryptophanase which converts tryptophan to indole, pyruvate, and ammonia were identified in association with two permease genes (Dhaf_1325 and Dhaf_2459). (screeninglibraries.com)

As a result, the expression of genes directly involved in colistin biosynthesis and secretion increased, indicating that at the transcriptional level spo0A and abrB played opposite roles in regulating colistin biosynthesis in P. polymyxa . (hindawi.com)
Many bioY genes are located at loci encoding biotin biosynthesis or are unlinked to either biotin synthesis or other biotin transport genes. (tcdb.org)

The depletion of DOM over time was strongly correlated with increases in expression of many genes associated with heterotrophy (e.g., amino acid, fatty acid, and carbohydrate metabolism) belonging to a Hydrogenophaga strain that accounted for a relatively large percentage (~8%) of the metatranscriptome. (frontiersin.org)
A basic pattern of gene expression and of relative expression levels during different growth phases was obtained for Corynebacterium glutamicum R grown on different carbon sources. (microbiologyresearch.org)
In addition, use of starch stimulated the expression of regulatory gene spo0A but repressed the expression of another regulatory gene abrB . (hindawi.com)
Expression of truncated bio transport operons, each with one gene deleted, resulted in stable BioMN complexes but revealed only low amounts of BioMY and BioNY aggregates in the absence of the respective third partner. (tcdb.org)

This Hydrogenophaga strain also expressed genes indicative of chemolithoautotrophy, including CO 2 fixation, H 2 oxidation, S-compound oxidation, and denitrification. (frontiersin.org)
To increase the variety of chemical products that A. woodii can produce, a novel strain was created using genes from another anaerobic bacterium, Clostridium acetobutylicum . (engicoin.eu)

The gene was neither found in the Y51 genome, nor in those of other members of the Peptococcaceae family listed in IMG (Integrated Microbial Genomes), suggesting that this group of organisms may have evolved an unconventional dihydrofolate reductase for the synthesis of THF. (screeninglibraries.com)

Nitrogen-fixation genes and nitrogenase activity in Clostridium acetobutylicum and Clostridium beijerinckii. (thecoffeeparlor.com)

1981. Jones and Woods (1986) have thoroughly documented its history of use … This plasmid seems to be contain nearly all genes involved with solvent production and is aptly named pSOL1. (thecoffeeparlor.com)
Edited by Mark Hower, student of Rachel Larsen and Kit Pogliano, From MicrobeWiki, the student-edited microbiology resource, "Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum. (thecoffeeparlor.com)

All genes involved in glycolysis were coordinately expressed in medium containing glucose. (microbiologyresearch.org)

When inserted into A. woodii , these genes allow it to produce acetone, a valuable industrial chemical, from waste CO 2 . (engicoin.eu)
Attempts at introducing vectors containing the Cas9-encoding gene into Acetobacterium woodii have previously been unsuccessful, largely due to the presence of a restriction- modification system recognition site and the toxicity of the nuclease itself. (plasmidvectors.com)
All A. woodii vectors (HERE) contain the catP gene for chloramphenicol or thiamphenicol selection and the pCD6 Gram-positive replicon. (plasmidvectors.com)

Clostridium acetobutylicum has played an important role in biotechnology throughout the 20th century. (thecoffeeparlor.com)

Did evolutionary processes really have the ability or forward-thinking insight to construct the metabolism in C. acetobutylicum ? (icr.org)

on genome sequence should be carefully addressed, as observed in Clostridium acetobutylicum where both functional oxidative and reductive TCA cycles were confirmed experimentally in contrast to the previous genomic interpretation [15]. (screeninglibraries.com)

In Clostridium acetobutylicum, the genes encoding γ-GCS and putative GS were found to be immediately adjacent by BLAST search, and had amino acid sequence homology with S. agalactiae γ-GCS-GS, respectively. (elsevierpure.com)

iCac802 spans 802 genes and includes 1,137 metabolites and 1,462 reactions, along with gene-protein-reaction associations. (psu.edu)

We confirmed that the proteins expressed from each gene showed γ-GCS and GS activity, respectively. (elsevierpure.com)

Furthermore, the C. difficile toxin genes (tcdA and tcdB) and their regulators (tcdR and tcdC) are direct CcpA targets. (studyres.com)

The gapA-pgk-tpi-ppc gene cluster was transcribed as a mono- or polycistronic mRNA, depending on the growth phase. (microbiologyresearch.org)

The acetyl-CoA degradation and related genes are shown in more detail (boxed). (screeninglibraries.com)

cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. (systemsbiology.net)

Biological hydrogen production by Clostridium acetobutylicum in an unsaturated flow reactor. (thecoffeeparlor.com)

C. acetobutylicum GS had broad substrate specificities and synthesized several kinds of γ-glutamyltripeptide, γ-Glu-Cys-X aa . (elsevierpure.com)

A genomic-library based discovery of a novel, possibly synthetic, acid-tolerance mechanism in Clostridium acetobutylicum involving non-coding RNAs and ribosomal RNA processing. (jefferson.edu)

Emended descriptions of Clostridium acetobutylicum and Clostridium beijerinckii, and descriptions of Clostridium saccharoperbutylacetonicum sp. (thecoffeeparlor.com)

The main module page also shows the location of these motifs within the upstream sequences of the module member genes. (systemsbiology.net)
cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. (systemsbiology.net)

The tricarboxylic acid cycle (TCA) of D. hafniense DCB-2 and Y51 appears incomplete since they lack the gene coding for 2-oxoglutarate Chlormezanone dehydrogenase, and the cycle lacks the anaplerotic glyoxylate bypass (Figure 2). (screeninglibraries.com)

Experiments to determine what causes degeneration have shown that pSOL1 contains four genes which are vital for alcohol and acetone production. (thecoffeeparlor.com)

EPA Clostridium acetobutylicum Final Risk Assessment Genetic Engineering of Clostridium acetobutylicum for Enhanced Production of Hydrogen Gas: Penn State University. (wikipedia.org)

d) pMTL-MPD24: positive control for cargo delivery at the pheA locus through carriage of the FAST reporter gene flanked by the thiolase ( thl ) promoter (P thl ) and pyrE-hydA (orotate phosphoribosyltransferase and hydrogenase I) terminator from Clostridium acetobutylicum . (plasmidvectors.com)

Clostridium acetobutylicum is an anaerobic, spore-forming bacterium with the ability to ferment starch and sugars into solvents. (igem.org)

Deletion of the cbeI gene removes a substantial barrier to routine DNA transformation and chromosomal modification of C. bescii . (biomedcentral.com)

The research team, led by Eleftherios (Terry) Papoutsakis, Unidel Eugene Du Pont Chair of Chemical and Biomolecular Engineering, studied interactions between Clostridium ljungdahlii and C. acetobutylicum . (azolifesciences.com)

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