• Acetyl-CoA synthetase or Acetate-CoA ligase is an enzyme (EC 6.2.1.1) involved in metabolism of acetate. (creative-enzymes.com)
  • High purity Acetyl-CoA synthetase (B. subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. (creative-enzymes.com)
  • One Unit of acetyl-CoA synthetase is defined as the amount of enzyme required to produce one μmole of NADH from NAD+. (creative-enzymes.com)
  • It is in the ligase class of enzymes, meaning that it catalyzes the formation of a new chemical bond between two large molecules. (creative-enzymes.com)
  • Other names in common use include acetyl-CoA synthetase (ADP-forming), acetyl coenzyme A synthetase (adenosine diphosphate-forming), and acetate thiokinase. (wikipedia.org)
  • Human SIR2A and yeast SIR2 proteins restored growth of SIR2-deficient S. enterica on acetate and propionate, suggesting that eukaryotic cells may also use SIR2 proteins to control the synthesis of acetyl-CoA by the level of acetylation of acetyl-CoA synthetases. (nyu.edu)
  • Consistent with this idea, growth of a quintuple sir2 hst1 hst2 hst3 hst4 mutant strain of the yeast Saccharomyces cerevisiae on acetate or propionate was severely impaired. (nyu.edu)
  • In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. (uniprot.org)
  • RESULTS: Transcriptomic analysis of the mutant deficient in this LuxR-type regulator showed that the acsA gene encoding acetyl-CoA synthetase was down-regulated. (yonsei.ac.kr)
  • Transcriptional control of the yeast acetyl-CoA synthetase gene, ACS1, by the positive regulators CAT8 and ADR1 and the pleiotropic repressor UME6. (nih.gov)
  • The ACS1 gene, encoding one out of two acetyl-CoA synthetase isoenzymes of Saccharomyces cerevisiae, is strictly regulated at the transcriptional level by the carbon source of the medium. (nih.gov)
  • CONCLUSION: This study indicates that the VarS/VarA system plays a role in V. vulnificus metabolism via regulating AcsR, which in turn controls acetate metabolism by activating the transcription of the acetyl-CoA synthetase gene. (yonsei.ac.kr)
  • To facilitate NAD(P)H supplementation, acetaldehyde dehydrogenase 6 (ALD6) and acetyl-CoA synthetase 1 (ACS1) were overexpressed in recombinant Saccharomyces cerevisiae harboring the Pichia stipitis xylose reductase (XR) gene. (elsevier.com)
  • Acetate accumulation was accounted for by strong down-regulation of the acetate CoA ligase gene (acs) in addition to up-regulation of the pta and ackA genes (involved in acetate production), thus conserving ATP while reducing flux through the TCA cycle. (up.ac.za)
  • Propionyl-CoA is an intermediate in the metabolism of propanoate. (hmdb.ca)
  • 2-oxobutyrate is ultimately decomposed by acetate-CoA ligase and produces ATP, thus contributing to ATP metabolism. (wikipedia.org)
  • Specifically, it integrates distributed information from the literature to provide a complete and detailed view for metabolic processes such as acetyl-CoA synthesis, pyruvate synthesis, glycolysis/gluconeogenesis, reductive tricarboxylic acid (RTCA) cycle, non-oxidative pentose phosphate pathway (NOPPP), nitrogen metabolism, amino acid metabolism, and nucleotide biosynthesis. (pubmedcentralcanada.ca)
  • Acetyl-CoA synthetase or Acetate-CoA ligase is an enzyme (EC 6.2.1.1) involved in metabolism of acetate. (creative-enzymes.com)
  • Considering the changes of cell growth, ethanol and acetate production, a remarkable enhancement of xylitol production by ACS1 overexpression seemed be ascribed to energy and NAD(P)H generation through a metabolism from acetaldehyde to acetyl-CoA and TCA cycle. (elsevier.com)
  • The possible role of a hydroxycinnamoyl-CoA thioesterase in phenylpropanoid metabolism is discussed. (ubc.ca)
  • In this report, we review diverse CO 2 assimilation pathways, acetate assimilation, carbohydrate catabolism, the tricarboxylic acid cycle and some key, and/or unconventional enzymes in central carbon metabolism of phototrophic microorganisms. (frontiersin.org)
  • An energy-conserving pyruvate-to-acetate pathway in Entamoeba histolytica. (wikipedia.org)
  • Pyruvate synthase and acetate:coenzyme A (CoA) ligase (ADP-forming) activities were detected only in heterotrophically grown cultures. (umass.edu)
  • Catalysis of the reaction: ATP + biotin + apo-(methylmalonyl-CoA:pyruvate carboxytransferase) = AMP + diphosphate + (methylmalonyl-CoA:pyruvate carboxytransferase). (leibniz-fli.de)
  • Below are the list of possible Acyl-CoA synthetase short-chain family products. (mybiosource.com)
  • Also known as Acyl-CoA synthetase short-chain family member 3, mitochondrial. (mybiosource.com)
  • In enzymology, an acetate-CoA ligase (ADP-forming) (EC 6.2.1.13) is an enzyme that catalyzes the chemical reaction ATP + acetate + CoA ⇌ {\displaystyle \rightleftharpoons } ADP + phosphate + acetyl-CoA The 3 substrates of this enzyme are ATP, acetate, and CoA, whereas its 3 products are ADP, phosphate, and acetyl-CoA. (wikipedia.org)
  • Ligase that catalyzes the synthesis of acetyl-CoA from acetate and coenzyme A. (roche.com)
  • Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. (uniprot.org)
  • An enzyme that catalyzes the conversion of acetate esters and water to alcohols and acetate. (bioportfolio.com)
  • It is in the ligase class of enzymes, meaning that it catalyzes the formation of a new chemical bond between two large molecules. (creative-enzymes.com)
  • Catalyzes the production of acetyl-CoA. (ymdb.ca)
  • ACS1_YEAST ] Catalyzes the production of acetyl-CoA. (proteopedia.org)
  • ACS catalyzes the two-step activation of acetate to acetyl-CoA: formation of an acetyl-AMP intermediate from acetate and ATP and the transfer of the acetyl group to CoA. (proteopedia.org)
  • T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighbouring 3'-hydroxyl and 5'-phosphate ends in double-stranded DNA. (roche.com)
  • ACSS2 / ACAS2 is a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. (lsbio.com)
  • In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA. (uniprot.org)
  • The product of this reaction, acetyl-CoA, is the starting point for the citric acid cycle. (wikipedia.org)
  • This was previously masked by the fact that recombinant 4CL could convert most hydroxycinnamic acids to hydroxycinnamoyl-CoA thioesters faster than the thioesterase could perform the reverse reaction. (ubc.ca)
  • Catalysis of the reaction: ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. (leibniz-fli.de)
  • Catalysis of the reaction: succinate + CoA + nucleotide triphosphate = nucleotide diphosphate + phosphate + succinyl-CoA. (leibniz-fli.de)
  • Catalysis of the reaction: ATP + succinate + CoA = ADP + succinyl-CoA + phosphate. (leibniz-fli.de)
  • The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. (lsbio.com)
  • ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. (uniprot.org)
  • The citric acid cycle begins with the transfer of a two-carbon acetyl group from acetyl-CoA to the four-carbon acceptor compound (oxaloacetate) to form a six-carbon compound (citrate). (wikipedia.org)
  • Acetylated citrate lyase, acetate:CoA ligase (AMP forming), and phosphoenolpyruvate carboxylase activities increased in autotrophic and acetate-containing cultures. (umass.edu)
  • These data suggest that citrate lyase and AMP-forming acetate:CoA ligase, but not ATP citrate synthase, work opposite citrate synthase to control the direction of carbon flow in the citric acid cycle. (umass.edu)
  • Acetyl-coenzyme A synthetase (ACS) belongs to the family of AMP-forming enzymes that also includes acyl-CoA synthetases, firefly luciferase, and nonribosomal peptide synthetases. (proteopedia.org)
  • The reactions of the cycle are carried out by eight enzymes that completely oxidize acetate (a two carbon molecule), in the form of acetyl-CoA, into two molecules each of carbon dioxide and water. (wikipedia.org)
  • The next circle … Table 4 Number of genes associated with the general COG functional categories Insights from the genome Annotation of the genome indicated that strain MZ1T has complete glycolytic and citric acid cycle pathways along with two complete acetate assimilation pathways with the key enzymes being acetate-CoA ligase and acetate kinase-phosphate acetyl transferase, respectively, thereby allowing MZ1T to utilize acetate as a carbon source [31]. (hormonessignaling.com)
  • Two independent pathways in Escherichia coli convert acetate to acetyl CoA: reversal of acetate production by phosphotransacetylase and acetate kinase, and the acetyl-CoA synthetase (Acs) pathway that scavenges acetate. (nih.gov)
  • Activates acetate so that it can be used for lipid synthesis or for energy generation. (abcam.com)
  • RLA controls the activities of many proteins, including the acetyl coenzyme A (acetyl-CoA) synthetase (Acs), by modulating the degree of Acs acetylation. (asm.org)
  • Provides the acetyl-CoA source for histone acetylation in the nucleus. (ymdb.ca)
  • P. islandicum was grown at 95 degrees C autotrophically, heterotrophically, and mixotrophically with acetate, H2, and small amounts of yeast extract and with thiosulfate as the terminal electron acceptor. (umass.edu)
  • The growth rates on H2 and 0.001% yeast extract with and without 0.05% acetate were the same, but the maximum concentration of cells was fourfold higher with acetate. (umass.edu)
  • There was no growth with acetate if 0.001% yeast extract was not present, and addition of H2 to acetate-containing medium greatly increased the growth rates and maximum concentrations of cells. (umass.edu)
  • Citrate synthase activity decreased in autotrophic and acetate-containing cultures compared to the activity in heterotrophic cultures. (umass.edu)
  • The activity of the AMP-forming acetyl-CoA synthetase is posttranslationally modified by the protein acetyltransferase Pat. (asm.org)
  • A protein complex that includes a ubiquitin-protein ligase and enables ubiquitin protein ligase activity. (leibniz-fli.de)
  • Među identificiranim proteinima, pojačanu ekspresiju u sesilnim stanicama imali su fosfofruktokinaza, alkohol dehidrogenaza, dugolančana-masna-kiaselina-CoA-ligaza, univerzalni stres protein i protuprijenosna podjedinica mnhE2, dok su fosforibozilglicinamid formiltransferaza i acetat kinaza bili pojačano eksprimirani u planktonsim stanicama. (nsk.hr)
  • Propionic aciduria is caused by an autosomal recessive disorder of propionyl coenzyme A (CoA) carboxylase deficiency (EC 6.4.1.3). (hmdb.ca)
  • Citric acid cycle in the hyperthermophilic archaeon Pyrobaculum islandicum grown autotrophically, heterotrophically, and mixotrophically with acetate. (umass.edu)
  • The citric acid cycle ( CAC ) - also known as the TCA cycle (tricarboxylic acid cycle) or the Krebs cycle [1] [2] - is a series of chemical reactions used by all aerobic organisms to release stored energy through the oxidation of acetyl-CoA derived from carbohydrates , fats , and proteins . (wikipedia.org)
  • Through catabolism of sugars, fats, and proteins, the two-carbon organic product acetyl-CoA (a form of acetate) is produced which enters the citric acid cycle. (wikipedia.org)
  • 4-coumarate:CoA ligase (4CL) activity is required for the activation of hydroxycinnamic acids prior to their being fed into the lignin specific branch pathway. (ubc.ca)
  • An iolR mutant strain displayed a growth defect in minimal medium containing 10 mM acetate, a condition under which RLA function is critical to control Acs activity. (asm.org)
  • In Salmonella enterica, SIR2 function was required for activity of the acetyl-CoA synthetase (Acs) enzyme. (nyu.edu)
  • After 10 minutes heat denaturation at +65°C ligase activity is stopped. (roche.com)
  • One unit T4 DNA Ligase is defined as the amount of enzyme which converts 1 nmol of [ 32 P] from pyrophosphate into Norit-absorbable material in 20 minutes at +37°C. (roche.com)