Loading...
  • polyclonal
  • Immunohistochemistry (below the yellow line) (G-O) was performed using an anti-apoC-II polyclonal antibody (G to I, K to M, O) or goat IgG as negative control (J, N). Positive signals (red) were found in secretory granule-like structures in distal epithelial cells on GD 19.5 (H) and PN0 (G) but not in later timepoints. (nih.gov)
  • macrophages
  • The major site of apoC-II mRNA synthesis (positive signal, blue) changed after birth (compare A to C). Positive signals were found in newly-formed septa (D, E) and macrophages (E) on PN 5. (nih.gov)
  • protein
  • Recently, we showed expression of apolipoprotein C-II (apoC-II), the essential cofactor of lipoprotein lipase (LPL), in the fetal mouse lung and found the protein on the day of the surge of surfactant synthesis (gestation day 17.5) in secretory granule-like structures in the distal epithelium. (nih.gov)
  • In the present study, we will answer the following questions: Does apoC-II protein localization change according to the stage of lung development, thus according to the need in surfactant? (nih.gov)
  • Do the sites of apoC-II and LPL gene expression change according to the stage of lung development and to protein localization? (nih.gov)
  • The present study investigated whether the sites of apoC-II and LPL mRNA and protein accumulation are regulated in the mouse lung between gestation day 15 and postnatal day 10. (nih.gov)
  • Distribution of apolipoprotein C-II mRNA and protein in the perinatal mouse lung. (nih.gov)
  • fatty
  • This study demonstrates that apoC-II and LPL mRNAs correlate temporally and geographically with surfactant lipid synthesis in preparation for birth and suggests that fatty acid recruitment from the circulation by apoC-II-activated LPL is regionally modulated by apoC-II secretion. (nih.gov)
  • expression
  • The major sites of apoC-II and LPL gene expression changed over time and were found mainly in the distal epithelium at the end of gestation but not after birth. (nih.gov)
  • proteins
  • Studying brain cells derived from a type of induced human stem cells, the researchers found that APOE4 promotes the accumulation of the beta amyloid proteins that cause the characteristic plaques seen in the brains of Alzheimer's patients. (scitechdaily.com)
  • 2.Next, we examined if such proteins were involved in the degeneration processes of neurons after ischemia and target deprivation. (nii.ac.jp)
  • The 3 lipin proteins (lipin 1, lipin 2, and lipin 3) each have PAP activity, but have distinct tissue distributions, with lipin 1 being the predominant PAP enzyme in many metabolic tissues. (jci.org)
  • TAG synthesis in small intestinal enterocytes utilizes 2-monoacylglycerol and does not require the PAP reaction, making the role of lipin proteins in enterocytes unclear. (jci.org)
  • type 2 diabet
  • His work may reveal new strategies to treat dysregulated nutrient metabolism and type 2 diabetes. (childrenshospital.org)
  • The last American Diabetes Association (ADA) technical review of exercise and type 2 diabetes (formerly known as non-insulin dependent diabetes) was published in 1990. (diabetesjournals.org)
  • Large clinical trials demonstrating that lifestyle interventions (diet and exercise) reduce incidence of type 2 diabetes in people with impaired glucose tolerance (IGT). (diabetesjournals.org)
  • Clinical trials showing effectiveness of resistance training (such as weight lifting) for improving glycemic control in type 2 diabetes. (diabetesjournals.org)
  • The present review focuses on type 2 diabetes. (diabetesjournals.org)
  • CMR Short Reviews The Concept of CMR Historical background on global cardiometabolic risk, epidemiological aspects of obesity and type 2 diabetes, ABCs of cardiovascular disease risk factors, intra-abdominal adiposity, metabolic syndrome and contribution to cardiometabolic risk. (amazonaws.com)
  • Activation of LXRs with the selective synthetic agonist T0901317 significantly decreases blood glucose levels in a rodent model of type 2 diabetes ( 4 ). (diabetesjournals.org)
  • apoC-II secretion
  • Taken together, our results suggested that fatty acid recruitment from the circulation by apoC-II-activated LPL could be regionally controlled by modulation of apoC-II secretion [ 12 ] for the purpose of surfactant synthesis. (biomedcentral.com)
  • This study demonstrates that apoC-II and LPL mRNAs correlate temporally and geographically with surfactant lipid synthesis in preparation for birth and suggests that fatty acid recruitment from the circulation by apoC-II-activated LPL is regionally modulated by apoC-II secretion. (nih.gov)
  • phospholipid
  • Lipin 2/3 deficiency caused phosphatidic acid accumulation and mammalian target of rapamycin complex 1 (mTORC1) activation, which were associated with enhanced protein levels of a key phospholipid biosynthetic enzyme (CTP:phosphocholine cytidylyltransferase α) and altered membrane phospholipid composition. (jci.org)
  • Impaired chylomicron synthesis in lipin 2/3 deficiency could be rescued by normalizing phospholipid synthesis levels. (jci.org)
  • These data implicate lipin 2/3 as a control point for enterocyte phospholipid homeostasis and chylomicron biogenesis. (jci.org)
  • lung
  • In the present study, we will answer the following questions: Does apoC-II protein localization change according to the stage of lung development, thus according to the need in surfactant? (nih.gov)
  • transient
  • Three different positive signals with the anti-apoA-II antibody were found: one transient signal in the nucleus of a portion of mesenchymal cells, a second at lower levels throughout the mesenchyme, and another in capillaries with a specific increase from gestation day 17.5/18.5. (biomedcentral.com)
  • surfactant
  • A noticeable increase in surfactant lipid content was measured before the end of gestation day 18, which correlates temporally with the presence of apoC-II in secretory granules in distal epithelium with no or small lumina but not with large lumina. (nih.gov)
  • We propose a model where apoC-II is retained in secretory granules in distal epithelial cells until the lumina reaches a minimum size, and is then secreted when the rate of surfactant production becomes optimal. (nih.gov)
  • SNPs
  • Six phenotypes were observed as a result of two single nucleotide polymorphisms (SNPs) at amino acid positions 112 and 158. (dovepress.com)
  • Despite having 80% power to detect associations that account for as little as 0.2% of the variance of BMI, none of the 10 SNPs were significantly associated with BMI at any age, although two SNPs showed trends in the expected direction. (biomedcentral.com)
  • plaque
  • A number of metals including Fe(II)/Fe(III), Al(III), Zn(II) and Cu(II) are found co-localised with ß-sheets of Aß42 in senile plaque cores in AD brain. (j-alz.com)
  • There are data from in vitro studies which may begin to explain some of these unanswered questions and in considering these I have summised that Al(III) and Fe(III)/Fe(II) are directly involved in the precipitation of ß-sheets of Aß42 in senile plaque cores whereas the presence of Cu(II) and Zn(II) is adventitious. (j-alz.com)
  • endogenous
  • B ) Localization of endogenous lipin 2 (green) and lipin 3 (red) in mouse duodenum shown by confocal fluorescence microscopy. (jci.org)
  • Pathogen-associated molecular patterns (PAMPs) and endogenous molecules created upon tissue injury, since called damage-associated molecular patterns (DAMPs), signal the threat of either infection or injury to the organism, independently of their non-self- or self-identity [ 2 - 5 ]. (hindawi.com)