Acceptor proteins. Medical search. Frequent questions

Unlike other family members, this enzyme shows exclusive specificity for partially GalNAc-glycosylated acceptor substrates and shows no activity with non-glycosylated peptides. (nih.gov)
We have determined crystal structures of human xylosyltransferase 1 (XT1) in complex with the sugar donor, UDP-xylose, and various acceptor peptides. (rcsb.org)
The core experimental aim of the project is to develop a new single-molecule spectroscopic method, dynamic single-molecule Metal Induced Energy Transfer (or dynamic smMIET), which will be suited to study the conformational dynamics of intrinsically disordered peptides (sequences of FG-repeat proteins from the nuclear pore), and of small folding protein motifs, in particular helix-turn-helix (HTH) and WW-domain motifs. (uni-goettingen.de)
This method ( 10 ) mea- chemical-induced skin sensitization is the ability of a chemical, sured the depletion of the peptides after treatment with excess either as such or after ex/in cutaneo activation, to covalently electrophile for 24 h and adopted the percent depletion (dp) as react with a carrier protein or peptide ( 6 ), resulting in an the reactivity index of a given chemical. (cdc.gov)

In two unrelated French families linked to CMH4, we found a mutation in a splice acceptor site of the MyBP-C gene, which causes the skipping of the associated exon and could produce truncated cardiac MyBP-Cs. (nih.gov)

A particular challenge is the synthesis of oligosaccharyltransferases (OSTs), which catalyze the attachment of glycans to specific amino acid residues in target proteins. (biorxiv.org)
A group of enzymes that are dependent on cyclic GMP and catalyzes the phosphorylation of serine or threonine residues of proteins. (harvard.edu)
The enzyme encoded by this gene controls the initiation step of mucin-type O-linked protein glycosylation and transfer of N-acetylgalactosamine to serine and threonine amino acid residues. (nih.gov)
GAG biosynthesis is initiated by peptide O-xylosyltransferases, which transfer xylose onto selected serine residues in the core proteins. (rcsb.org)
This cylinder-shaped protein is composed of 11 (mostly) antiparallel β strands (1 and 6 are parallel) and an alpha helix through its center containing the chromophore that is formed autocatalytically from residues Ser65-Tyr66-Gly67. (ibs.fr)
Moreover, slo1 proteins provide sites that seem to recognize cholesterol and PIP2: seven CRAC motifs in the slo1 cytosolic tail and a string of positively charged residues (Arg329, Lys330, Lys331) immediately after S6, respectively. (bvsalud.org)
If the total charge of the protein is very negative, this may be an indication that many His residues may be HIP. (lu.se)

A constriction within the peptide binding cleft requires the acceptor serine to be followed by glycine or alanine. (rcsb.org)
The biosynthesis of GAG chains is initiated by xylosylation of a serine residue of the core protein, followed by galactosylation by beta 4GalT7. (lu.se)

Add DONOr and ACCEptor statements as needed to your RTF and regenerate the PSF - do not play directly with the PSF. (charmm.org)
The new technique, 'FRET X' will make use of the binding of DNA strands that are labelled (with donor and acceptor fluorophores) to build FRET fingerprints. (tudelft.nl)

In enzymology, a cellobiose dehydrogenase (acceptor) (EC 1.1.99.18) is an enzyme that catalyzes the chemical reaction cellobiose + acceptor ⇌ {\displaystyle \rightleftharpoons } cellobiono-1,5-lactone + reduced acceptor Thus, the two substrates of this enzyme are cellobiose and acceptor, whereas its two products are cellobiono-1,5-lactone and reduced acceptor. (wikipedia.org)
A novel human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, GalNAc-T7, with specificity for partial GalNAc-glycosylated acceptor substrates. (nih.gov)
The biosynthesis can also be initiated by exogenously added beta-D-xylopyranosides with hydrophobic aglycons, which thus can act as acceptor substrates for beta 4GalT7. (lu.se)

Acetate kinase was selected as a control for intracellular proteins. (rawveronica.com)
Vascular smooth muscle cell contractile protein expression is increased through protein kinase G-dependent and -independent pathways by glucose-6-phosphate dehydrogenase inhibition and deficiency. (harvard.edu)

Cofactors are protein molecules which assist enzyme function. (flashcardmachine.com)
Our studies have revealed a surprise: the protein nanowires have a core of metal-containing molecules called hemes .By "sequencing" with cryo-electron microscopy, we found that hemes line up to create a continuous path along which electrons travel. (yale.edu)
The barrel structure produces a very tight packing of the interior of the protein, so that the chromophore and water molecules are largely fixed into place by hydrogen bonds, with limited (but not impossible) diffusion of ions or small molecules. (ibs.fr)
One means through which AGEs modulate cellular functions is through binding to specific cell surface acceptor molecules. (diabetesjournals.org)
SmMIET is based on the distance-dependent quenching of the fluorescence emission of fluorescent molecules by metallic or semi-metallic thin films, and it requires labeling of the peptide/protein of interest by only one dye, in contrast to single-molecule Förster Resonance Energy Transfer, which requires labeling with a donor and an acceptor dye label. (uni-goettingen.de)

A novel splice acceptor mutation in the DSPP gene causing dentinogenesis imperfecta type II. (medlineplus.gov)

I will start by outlining the most common problems encountered during the expression of recombinant proteins in E. coli and specific solutions will be given for each problem. (neb.com)
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. (rndsystems.com)

Fluorescently-tagged proteins are ideal reporters for studying the physiological status of living cells. (uni-marburg.de)
Protein diffusion in living cells is effectively measured by fluorescence correlation spectroscopy (FCS), a fluctuation-based approach that allows to carry out measurements at a physiological protein expression level. (uni-marburg.de)

This enzyme is a type II transmembrane protein and shares common sequence motifs with other family members. (nih.gov)
Numerous transmembrane protein ion channels have now been observed to utilize hydrophobic gating in their activation and regulation. (bvsalud.org)

A CALMODULIN-dependent enzyme that catalyzes the phosphorylation of proteins. (bvsalud.org)

Mutations in the cardiac MyBP-C gene likely cause chromosome 11-linked hypertrophic cardiomyopathy, further supporting the hypothesis that hypertrophic cardiomyopathy results from mutations in genes encoding contractile proteins. (nih.gov)
Deep sequencing has revealed thousands of eukaryotic protein-coding genes that defy the central dogma, producing circular noncoding RNAs rather than linear messenger RNAs. (bcm.edu)
For some genes, the abundance of the circular RNA exceeds that of the associated linear mRNA by a factor of 10, raising the interesting possibility that the function of some protein-coding genes may actually be to produce circular noncoding RNAs, not proteins. (bcm.edu)
Using RNAi screening in Drosophila cells, we identified many hnRNPs, SR proteins, core spliceosome, and transcription termination factors that control the outputs of reporter and endogenous genes. (bcm.edu)
In total, these results indicate that inhibition or slowing of canonical pre-mRNA processing events shifts the steady-state output of protein-coding genes towards circular RNAs, which likely helps explain why and how circular RNAs show tissue-specific expression profiles. (bcm.edu)

The observed GMC-oxidoreductase protein fold features an FAD-binding and a substrate-binding subdomain. (wikipedia.org)
In the closed-state the haem b accepts an electron from the FAD after substrate oxidation and it donates the electron to an external electron acceptor in the open-state. (wikipedia.org)
Xylose appears to be the optimal acceptor substrate for galactosylation by beta 4GalT7. (lu.se)

This protein fold was a new fold observed for a cytochrome. (wikipedia.org)
Cyt c554 functions in the latter process by accepting pairs of electrons from HAO and transferring them to a cytochrome acceptor. (rcsb.org)

The systematic name of this enzyme class is cellobiose:acceptor 1-oxidoreductase. (wikipedia.org)

cGMP via PKG activates 26S proteasomes and enhances degradation of proteins, including ones that cause neurodegenerative diseases. (harvard.edu)
Donor-Acceptor Distance Sampling Enhances the Performance of "Better than Nature" Nicotinamide Coenzyme Biomimetics. (manchester.ac.uk)
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (rndsystems.com)

NfsB is a dimeric flavoprotein enzyme that has broad electron acceptor specificity 15 and has been used in conjunction with prodrugs to selectively ablate cells in vertebrates. (cdc.gov)

G protein-coupled receptor signaling is known to govern the hypertrophic response through the regulation of ion channel activity and downstream signaling in failing cardiomyocytes. (duke.edu)
OBJECTIVE: Transient receptor potential canonical (TRPC) channels are G protein-coupled receptor operated channels previously implicated in cardiac hypertrophy. (duke.edu)
Kim E, Cho KO, Rothschild A, Sheng M. Heteromultimerization and NMDA receptor-clustering activity of Chapsyn-110, a member of the PSD-95 family of proteins. (umassmed.edu)
Studies of the assembly and plasma membrane trafficking of G protein-coupled receptor (GPCR) signalling complexes will play an important role in the development of new drugs with fewer side effects. (umontreal.ca)

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for HOS4 . (yeastrc.org)
Researcher Chirlmin Joo of TU Delft will receive a VICI grant for developing a method to sequence proteins one at a time. (tudelft.nl)
This can be done by sequencing: the determination of the amino-acid sequence of a protein since each protein has a unique amino acid sequence. (tudelft.nl)
Small sample size, with the high number of cellular proteins, and the necessity to distinguish 20 different amino acids are only a few of the obstacles currently limiting our ability to sequence proteins. (tudelft.nl)
Joo therefore proposes a novel sequencing technique that probes the protein profile to retrieve the sequence. (tudelft.nl)
FRET X will generate higher resolution data than standard FRET, and these will then be compared with protein databases for sequence prediction. (tudelft.nl)

A model metal-reducing bacterium with the capacity of reducing both insoluble and soluble metals can be strains, may use electrode areas as terminal electron acceptors also, allowing era of energy [8]C[10]. (rawveronica.com)
How do bacteria breathe without oxygen or soluble electron acceptors? (yale.edu)

Cyclic GMP-Dependent Protein Kinases" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
This graph shows the total number of publications written about "Cyclic GMP-Dependent Protein Kinases" by people in Harvard Catalyst Profiles by year, and whether "Cyclic GMP-Dependent Protein Kinases" was a major or minor topic of these publication. (harvard.edu)
Below are the most recent publications written about "Cyclic GMP-Dependent Protein Kinases" by people in Profiles. (harvard.edu)

Tunneling analysis that takes the dynamical fluctuations of the macromolecules into explicit account provides a unified view that links structure and function in protein electron transfer. (aps.org)
Protein diffusion plays a crucial role in determining what function a protein serves within the cell and how, when and where it may physically interact with other proteins and macromolecules in response to external stimuli. (uni-marburg.de)

Structures, functions and electron transfer mechanisms of proteins nanowires. (yale.edu)
Mechanism and energetics of green fluorescent protein chromophore synthesis revealed by trapped intermediate structures. (proteopedia.org)
Barondeau DP, Putnam CD, Kassmann CJ, Tainer JA, Getzoff ED. Mechanism and energetics of green fluorescent protein chromophore synthesis revealed by trapped intermediate structures. (proteopedia.org)

Regulating protein breakdown through proteasome phosphorylation. (harvard.edu)

Metronidazole is heterocyclic nitro derivative in which the nitro group of metronidazole is reduced by an electron transport protein such as NADPH in an anaerobic micro-organism [6,7,12,13]. (who.int)

446 013 single nucleotide variants which alter the codon to make an altered amino acid in the protein product. (lu.se)

This reactivity of chemicals to cutaneous the peptide reactivity assay ( 10 ), quantitative LC-MS was proteins is the basis for most, if not all, current nonanimal- exploited by Natsch et al. (cdc.gov)

strain CBDB1 used hexachlorobenzene and pentachlorobenzene as electron acceptors in an energy-conserving process with hydrogen as electron donor. (ufz.de)
Poor coupling across nonbonded interfaces produces a strong bias in favor of covalent and hydrogen-bonded pathways between redox sites in proteins. (aps.org)

A low-molecular-weight, non-protein organic compound participating in enzymatic reactions as dissociable acceptor or donor of chemical groups or electrons. (ebi.ac.uk)
Deep in the ocean or underground, where there is no oxygen, diverse microbes "breathe" by projecting tiny protein filaments called "nanowires" into the soil, to dispose of excess electrons resulting from the conversion of nutrients to energy. (yale.edu)
We have found a novel electron escape route in proteins to avoid oxidative damage ( PNAS 2021) and how cooling speeds up electrons ( Science Adv . 2022). (yale.edu)
Reactions in which electrons tunnel long distances from donors (D) to acceptors (A) pervade solid-state physics, chemistry and biology. (aps.org)

In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. (nih.gov)

Specifically, by using lipid nanodiscs as cellular membrane mimics, we obtained yields of up to 440 µg/mL for the single-subunit OST enzyme, 'Protein glycosylation B' (PglB) from Campylobacter jejuni , as well as for three additional PglB homologs from Campylobacter coli, Campylobacter lari , and Desulfovibrio gigas . (biorxiv.org)
The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. (nih.gov)
PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity. (nih.gov)
Their activity, however, is suited to tissue-specific physiology largely due to their association with regulatory subunits (ß and Î³ types), chaperone proteins, localized signaling, and the channel's lipid microenvironment. (bvsalud.org)
S-Acylation, the reversible post-translational lipid modification of proteins, is an important mechanism to control the properties and function of ion channels and other polytopic transmembrane proteins. (bvsalud.org)

This is the first report that proposes a role for a multicopper oxidase-like protein in an anaerobic organism. (nih.gov)

Oxygen is the electron acceptor and is reduced to water. (sigmaaldrich.com)
One of the most interesting features of the GFP chromophore is that it does not require a catalytic system to be formed, but is autocatalytically assembled after protein production, requiring only molecular oxygen as a cofactor for its full maturation. (ibs.fr)

This technique for detecting protein BMS 599626 in unchanged conductive biofilms works with a model where in fact the usage of redox protein adjustments with depth. (rawveronica.com)
The coupling disparity between bonded and nonbonded interfaces accounts in large part for the finding that protein electron-transfer rates do not exhibit a uniform dependence on distance, but instead depend critically on the composition of the medium between redox sites. (aps.org)

Important cytoprotective mechanisms activated by oxidative inflammatory conditions are mediated by nitrated fatty acids that covalently modify proteins to limit inflammation and oxidant stress. (aspetjournals.org)
The crystal structure of cyt c554 at 2.6 A resolution shows a predominantly alpha-helical protein with four covalently attached hemes. (rcsb.org)

This function represents one of the most important posttranslational modification of proteins. (sav.sk)
After sample fixation and cell wall permeabilization, leaves are treated with Sytox Orange, a nucleic acid dye that can function as a FRET acceptor. (nature.com)
Hudson, W.H. & Ortlund, E.A. The structure, function and evolution of proteins that bind DNA and RNA. (nature.com)
This protein may function as a follow-up enzyme in the initiation step of O-glycosylation. (nih.gov)
I am a plant cell biologist and protein biochemist at Oxford Brookes University with expertise in the structure and function of the plant endoplasmic reticulum (ER), membrane proteins and auxin biosynthesis using biochemical techniques as well as high-resolution live cell imaging. (brookes.ac.uk)
As the design and function of signaling networks depends on protein-protein interactions, which in turn are affected by protein diffusion, resolving such properties in space and time helps to understand signaling up to system-level properties such as signal integration and amplification. (uni-marburg.de)

The PGs consist of a core protein, with one or several GAG chains attached. (lu.se)

DSPP gene mutations alter the proteins made from the gene, leading to the production of abnormally soft dentin. (medlineplus.gov)

Recently the human cardiac myosin binding protein-C (MyBP-C) gene was mapped to chromosome 11p11.2 (ref. 8), making this gene a good candidate for the fourth locus, CMH4 (ref. 5). (nih.gov)
A second group of methods, which includes DNA microarrays and proteomics, have advantages that overcome the limitations implicit in signature-tagged mutagenesis and in vivo expression technology, namely, the ability to directly measure expression (gene or protein) levels on a true genome-wide scale, but their application to analysis of bacterial pathogens during real infections is still in its infancy. (cdc.gov)
956 958 transcript variants of a non-coding RNA gene (located within a gene that does not code for a protein). (lu.se)
The DSPP gene provides instructions for making two proteins that are essential for normal tooth development. (medlineplus.gov)

To illustrate our approach, we provide experimental procedures to detect the interaction between plant DNA and two proteins (the AeCRN13 effector from the oomycete Aphanomyces euteiches and the AtWRKY22 defensive transcription factor from Arabidopsis ). (nature.com)
Tools for this purpose have already been developed, but a more versatile tool which would allow assessment of multiple aspects of GPCR trafficking and protein/protein interaction could greatly facilitate and expedite these studies. (umontreal.ca)

PDE1 inhibition facilitates proteasomal degradation of misfolded proteins and protects against cardiac proteinopathy. (harvard.edu)

Motion, G.B., Howden, A.J.M., Huitema, E. & Jones, S. DNA-binding protein prediction using plant specific support vector machines: validation and application of a new genome annotation tool. (nature.com)
The current study was undertaken to elucidate the relative utility of these prediction tools over a range of drug lipophilicity and microsomal protein concentration. (aspetjournals.org)

Here we show that ABHD17a (α/ß-hydrolase domain-containing protein 17a) deacylates the stress-regulated exon domain of large conductance voltage- and calcium-activated potassium (BK) channels inhibiting channel activity independently of effects on channel surface expression. (bvsalud.org)

My additional independent research on ER localisation and splicing in auxin biosynthesis showed for the first time ER-localisation for an auxin biosynthetic protein. (brookes.ac.uk)

OmpB is predicted to be a multicopper protein, with highest homology to the manganese oxidase, MofA, from Leptothrix discophora. (nih.gov)

And then Mehmet Berkman will highlight the SHuffle line of E. coli strains for producing proteins with complex disulphide bonds. (neb.com)

I am committed to interdisciplinary research, and an example of the successes gained from this approach is the project with Prof A Nabok (Engineering Sheffield Hallam University) using total internal reflection ellipsometry to quantify protein-membrane interactions on native plant membranes and human cell lines. (brookes.ac.uk)
In order to fully understand biological processes, it is critical to be able to identify and quantify the proteins in cells. (tudelft.nl)

Program access grant to the STFC Harwell Laser Facility 'The Plant Cell Initiative: Protein interactions in the higher plant secretory pathway' 2017-2021 (approximate value of £200K). (brookes.ac.uk)

Following me will be Corinna Tuckey, who will describe the utility and advantages of the PURExpress in vitro protein synthesis kit. (neb.com)
NEB also offers solutions for expressing these types of proteins, either in vitro or in vivo. (neb.com)
Various experimental approaches exist for the study of nucleic acid-protein interactions in vitro and in vivo , but the detection of such interactions at the subcellular level remains challenging. (nature.com)

I published the first report of plant ER reticulon protein interactors by Co-IP and FRET-FLIM. (brookes.ac.uk)

Here we describe how to detect nucleic acid-protein interactions in plant leaves by using a fluorescence resonance energy transfer (FRET) approach coupled to fluorescence lifetime imaging microscopy (FLIM). (nature.com)

[7] However, when growing with sulfur as the final electron acceptor, no ATP is produced. (eol.org)

Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. (proteopedia.org)

Protein glycosylation, or the attachment of sugar moieties (glycans) to proteins, is important for protein stability, activity, and immunogenicity. (biorxiv.org)
Furthermore, we demonstrate the ability of cell-free synthesized OSTs to glycosylate multiple target proteins with varying N -glycosylation acceptor sequons. (biorxiv.org)

If a membrane protein expresses at a high level, almost certainly some of this protein will be found in the insoluble fraction. (neb.com)

Engineered resonance energy transfer (RET) between a mTurquoise2 fluorescent protein and FMN-bound As LOV2 increases switch sensitivity. (nature.com)
This generalises to other fluorescent protein tags and As LOV2-based actuators. (nature.com)
As shown on the figure (so called Jablonski diagram), when a molecule (e.g. a fluorescent protein) in the electronic ground state absorbs a photon of suitable wavelength, it accesses the first electronically excited state. (ibs.fr)
For FPs, one often compares the MB to that of enhanced Green Fluorescent Protein (EGFP), which is about 30000 M -1 .cm -1 . (ibs.fr)
Green fluorescent protein has revolutionized cell labeling and molecular tagging, yet the driving force and mechanism for its spontaneous fluorophore synthesis are not established. (proteopedia.org)

Protein-protein interactions in living cells are monitored in a quantitative time- and space-resolved fashion by the microscopy-based Förster Resonance Energy Transfer (FRET) approach. (uni-marburg.de)
Skruzny M, Pohl E, Gnoth S, Malengo G, Sourjik V (2020) The protein architecture of the endocytic coat analyzed by FRET microscopy. (uni-marburg.de)

Here, we address this challenge by establishing a bacterial cell-free protein synthesis platform that enables rapid production of a variety of OSTs in their active conformations. (biorxiv.org)

Anatomy2

Organisms11

Chemicals and Drugs103

Analytical, Diagnostic and Therapeutic Techniques and Equipment18

Phenomena and Processes46

Disciplines and Occupations1

Information Science5

Health Care1

Peptides4

Splice acceptor site1

Residues7

Serine2

DONOr and ACCEptor2

Substrates3

Kinase2

Molecules5

Mutation1

Recombinant proteins2

Physiological2

Transmembrane protein2

Catalyzes the phosphorylation1

Genes5

Substrate3

Cytochrome2

Oxidoreductase1

Enhances3

Specificity1

Receptor4

Sequence6

Soluble2

Kinases3

Macromolecules2

Structures3

Phosphorylation1

Electron Transport1

Amino acid1

Peptide1

Hydrogen2

Electrons4

Cholesterol1

Lipid5

Anaerobic1

Oxygen2

Redox2

Covalently2

Function6

Chains1

Mutations1

Gene4

Interaction2

Cardiac1

Prediction2

Exon1

Biosynthesis1

Oxidase1

Strains1

Quantify2

20211

Vitro3

FRET-FLIM1

Nucleic acid1

Sulfur1

Aequorin1

Glycosylation2

Membrane protein1

Fluorescent protein5

Microscopy2

Synthesis1