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  • Fluorescent In Situ Hybri
  • The peptide nucleic acid fluorescent in situ hybridization (PNA FISH) test for Enterococcus faecalis and other enterococci (EFOE) is a multicolor probe that differentiates E. faecalis from other enterococcal species within 3 h directly from blood cultures demonstrating gram-positive cocci in pairs and chains (GPCPC). (asm.org)
  • Derivatives
  • In the present communication, we have conjugated five different MTD peptides, penetratin, tat peptide, transportan-27, and two of its truncated derivatives, transportan-21 and transportan-22, to a 16mer PNA targeted to the TAR region of the HIV-1 genome. (pubmedcentralcanada.ca)
  • The review illustrates the structural requirements of a noteworthy peptide TAT-PTD and other derivatives chiefly to exemplify their implication in gene therapy. (ovid.com)
  • A 80 KDa poly(methyl vinyl ether-alt-maleic acid) (PA) polymer was conjugated with multiple copies of PNA and with multiple copies of poly(ethylene glycol) (PEG) by reacting the NHS derivative of PA with the amine derivatives of PNA and PEG. (umassmed.edu)
  • Proteins
  • The role of peptides in the interrelations between the structure and function of proteins is widely investigated, especially by using computer-assisted methods. (medsci.org)
  • Fusion proteins comprising non-IL-15 signal peptide linked to IL-15 protein sequences and fusion proteins comprising an IgE signal peptide linked to non-IgE protein sequences are disclosed. (google.com)
  • polyamide
  • Earlier, we have shown that an anti-TAR polyamide nucleotide analog (PNA TAR ) conjugated to a membrane transducing (MTD) peptide, transportan, is efficiently taken up by the cells and displays potent antiviral and virucidal activity [B. Chaubey, S. Tripathi, S. Ganguly, D. Harris, R. A. Casale and V. N. Pandey (2005) Virology , 331, 418- (pubmedcentralcanada.ca)
  • detection
  • The aim of this study is to prove higher detection rate of EGFR mutation with peptide nucleic acid (PNA) clamping compared to direct sequencing. (aacrjournals.org)
  • The model reported here has a potential capability of applying in developing PNA based biosensors for specific nucleic acid detection at room temperature. (nsti.org)
  • Abstract 62: Detection and comparison of EGFR mutations in matched tumor tissue, cell block, pleural effusion and serum with PNA clamping and direct sequencing in NSCLC with malignant pleural effusion. (aacrjournals.org)
  • The objective of this research project is to develop an electrochemical nucleic acid hybridization assay that is sensitive (picomole to femtomole detection level), stable with storage (6 months), portable, and easy to use in a simple assay format. (epa.gov)
  • probes
  • A PNA array prepared using the PNA conjugated with multi-amine linkers exhibits improved sensitivity and specificity of signals for detecting target nucleic acids as compared to a PNA array using PNA probes having only one amine group. (patentsencyclopedia.com)
  • Our protocol includes details on procedures to isolate mitotic chromosomes from adherent cell cultures, FISH using peptide nucleic acid (PNA) probes on chromosomes in suspension and flow cytometry setup and data acquisition. (scienceexchange.com)
  • ligands
  • Use of the post-insertion technique to insert peptide ligands into pre-formed Stealth liposomes with retention of binding activity and cytotoxicity" Pharmaceutical Research 19(3):265-269, 2002. (patentgenius.com)
  • monomers
  • In this study, RAFT polymerization of three monomers with markedly different properties, N , N -dimethylaminoethyl methacrylate (DMAEMA, cationic), n -butyl methacrylate (BMA, hydrophobic) and oligo(ethylene glycol) methyl ether methacrylate (OEGMA, hydrophilic) was conducted using an RGD peptide based RAFT agent as a chain transfer agent (RGD-CTA). (rsc.org)
  • Ligation
  • After ligation to the modular peptide-based BioShuttle carrier, which was utilized to facilitate the delivery of the functional modules into the cells' cytoplasm, the modules were scrutinized. (medsci.org)
  • clinically
  • Feasible patient-specific theranostic platforms in the personalized medicine are particularly dependent on efficient and clinically applicable peptide constructs. (medsci.org)
  • Blocks
  • A new method to install benzyl groups at the 4- and 6-positions on the trichloroacetyl protected glucosamine and at the 2- and 3-positions on glucuronic acid allowed the synthesis of the more electron rich hyaluronic disaccharide building blocks. (iastate.edu)
  • Linker
  • With a disulphide dimethacrylate (DSDMA) redox-cleavable cross-linker, the above linear polymers were combined via an "arm-first" technique to access MAS polymers containing peptide arms. (rsc.org)
  • Biological
  • In summary, we explore strategies to improve the peptide-mediated delivery of ASOs and small molecule chemotherapeutics across biological barriers. (mit.edu)
  • virucidal
  • These results indicate that anti-HIV-1 PNA conjugated with MTD peptides are not only inhibitory to HIV-1 replication in vitro but are also potent virucidal agents which render HIV-1 virions non-infectious upon brief exposure. (pubmedcentralcanada.ca)
  • Methods
  • DAPI nucleic acid stain and fluorescine labeling methods were used to prove the binding of PNA with its cDNA on nano FET surface from 10 nM solution, using fluorescent microscopic imaging. (nsti.org)
  • Here we explore methods for the peptide-mediated delivery of antisense oligonucleotides (ASOs) and chemotherapeutics. (mit.edu)