Abstract form biofilms. Medical search. Frequent questions

Using multidrug-resistant A. baumannii strains, an antibiotic susceptibility test was performed using the Gram-negative identification card of the Vitek 2 system (bioMérieux Inc., France), as well as an analysis of resistance genes, the effects of treatment with a light-emitting diode (LED) array using Radachlorin (RADA-PHARMA Co., Ltd., Russia), and transmission and scanning electron microscopy to confirm the biofilm-inhibitory effect of PDT. (ophrp.org)
To corroborate these results, biofilms were observed by scanning electron microscopy (SEM) and confocal microscopy. (biomedcentral.com)

Bacteria form multicellular communities known as biofilms that cause two thirds of all infections and demonstrate increased adaptive resistance to conventional antibiotics. (ubc.ca)
Historically, multicellular bacterial communities, known as biofilms, have been thought to be held together solely by a self-produced extracellular matrix. (nature.com)
A major virulence factor of Candida is its ability to adapt to a variety of different habitats and the consequent formation of surface-attached microbial communities known as biofilms [ 5 ]. (biomedcentral.com)

In addition, 10 clinical NTHi isolates from patients with CF formed biofilms on plastic surfaces. (nih.gov)
These biofilms exhibited decreased susceptibility to antibiotics and were adherent to epithelial surfaces. (nih.gov)
The ability of Streptococcus mutans to adhere to oral surfaces and form biofilm is a key step in the tooth decay process. (cranberryinstitute.org)
There was no significant difference observed in the density of biofilms formed by isolates from different sources but out of the 9 isolates tested previously for the presence of adhesin genes (virulence factors/appendages that facilitates bacteria attachment to host cell/surfaces), moderate biofilm producers harbored multiple adhesin genes whilst weak biofilm producers harbored only one adhesin gene each. (lincoln.ac.uk)
Microbial biofilms forming on marine surfaces are at the root of biofouling problems. (omicsonline.org)
Of particular interest is the occurrence of dispersal events where a subpopulation of cells is released from the biofilm community to colonize new surfaces. (omicsonline.org)
We applied this strategy to study coagulase (Coa), a secreted protein and a major contributor to the formation of a fibrin network in S. aureus biofilms that protects bacteria from the host immune system and increases attachment to host surfaces. (sdu.dk)
Streptococcus mutans , a representative bacteria of tooth decay [ 10 ], forms plaques (biofilm) on tooth surfaces and produces lactate from sugars such as sucrose, which demineralizes teeth and causes dental caries. (biomedcentral.com)
Methods and Results: Biofilm formation on plastic and cFn-coated surfaces by the wild-type M1 and M41 strains, and their isogenid sc/1-inactivated mutants, we compared spectrophotometrically following crystal violet staining. (cdc.gov)
This development is conditioned by complex processes involving bacterial attachment to surfaces, growth, cell-to-cell communication, mobility and production of exoproducts constituting the biofilm matrix. (ifremer.fr)
Many bacteria grow on surfaces forming biofilms. (helmholtz-hzi.de)
In order to form the fruiting bodies, M. xanthus cells must be able to move across solid surfaces. (syr.edu)
Imaging of biofilms directly on catheter surfaces, along with quantification of levels of encrustation and biomass, confirmed that the mutants were attenuated specifically in the ability to form crystalline biofilms compared with that of the wild type. (brighton.ac.uk)
The ability of bacteria to attached on surfaces and form a distinct biofilm can lead to food spoilage. (uthm.edu.my)

Biofilm production for 182 MRSA isolates obtained from clinical culture sites (2004 to 2013) was quantified. (uri.edu)
Of the isolates evaluated, 25.8% (47/182) produced strong biofilms and 40.7% (74/182) produced weak biofilms. (uri.edu)
One of the objectives of this study was to compare the biofilm-forming potential of C. perfringens isolates from broiler chickens, free-range poultry environments and neonatal humans using the traditional protocols of crystal violet staining assays in microtiter plates. (lincoln.ac.uk)
All fifty-four (54) C. perfringens isolates tested from a variety of sources were shown to form biofilms. (lincoln.ac.uk)
We compared oral and systemic isolates for the capacity to form biofilm in an in vitro biofilm model and pathogenicity in the Galleria mellonella infection model. (biomedcentral.com)
The biofilm formation and pathogenicity in Galleria was similar between oral and systemic isolates. (biomedcentral.com)
Importantly, for C. albicans the pathogenicity of oral Candida isolates was similar to systemic Candida isolates, suggesting that Candida isolates have similar biofilm-forming ability and virulence regardless of the infection site from which it was isolated. (biomedcentral.com)
Objectives: Biofilm formation (BF) by fungal isolates may dramatically complicate infection. (unicatt.it)
We determined the ability of Candida parapsilosis isolates from single fungaemia episodes to form biofilms and we analysed biofilm subgroups for antifungal susceptibility and pathogenic potential. (unicatt.it)
Additionally, the biofilm inhibitory concentration values of neofiscalin A against the MRSA and VRE isolates were 96 and 80 μg mL(-1), respectively. (nih.gov)
The biofilm screening of 58 isolates exhibited strong biofilm formation by 17 strains, moderate biofilm formation by 15 strains, weak biofilm formation by 21 strains, and no biofilm formation by 5 strains. (nitrkl.ac.in)

Logistic regression was used to quantify the effect of strong biofilm production (versus weak biofilm production) on clinical outcomes and independent predictors of a strong biofilm. (uri.edu)
95% CI, 0.002 to 0.39) were less likely to harbor strong biofilm-producing MRSA. (uri.edu)
7/54 (13%) were strong biofilm producers, 31/54 (57%) were moderate biofilm producers and 16/54 (30%) were weak biofilm producers according to current criteria. (lincoln.ac.uk)
Out of the 17 strong biofilm former, 8 bacterial strains exhibited tolerance to high concentrations of Cr, Pb, and Cd, i.e., ˃500 mg/L. The potent multi-metal resistant biofilm-forming bacterial strains were identified as Pseudomonas aeruginosa OMCS-1, Staphylococcus sp. (nitrkl.ac.in)

The aim of this study was to investigate a berry (wild blueberry, cranberry, and strawberry) polyphenolic fraction, commercialized as OrophenolR, for its antibacterial, anti-biofilm, and anti-adhesion properties on S. mutans. (cranberryinstitute.org)
Additional screens identified 1018 as a potent anti-biofilm peptide that prevented biofilm formation and led to the eradication of mature biofilms in both Gram-negative and Gram-positive bacteria. (ubc.ca)
Further studies identified even more potent D-enantiomeric anti-biofilm peptides DJK-5 and DJK-6 that also prevented (p)ppGpp accumulation, were highly synergistic with conventional antibiotics and exhibited in vivo activity. (ubc.ca)
This has led to vast increase in research to identify new (and sometimes old) anti-biofilm strategies that demonstrate effectiveness against these tolerant phenotypes. (edu.au)
Furthermore, many biofilm models testing anti-biofilm strategies aimed at the medical arena have not considered the matter of relevance to an intended application. (edu.au)
Expert commentary: This review will explore the issues that need to be considered in developing performance standards for anti-biofilm therapeutics and provide a rationale for the need to standardize models/methods that are clinically relevant. (edu.au)

In addition, the susceptibility of C. perfringens to a range of veterinary or human antimicrobial drugs in conventional or biofilm modes were tested using the broth microdilution method. (lincoln.ac.uk)
Traditional antimicrobial susceptibility studies in clinical microbiology laboratories have lied on the study of planktonic form of microorganisms. (cun.es)
Microbiological diagnosis and antimicrobial susceptibility studies of biofilm-related infections are complex and, nowadays, represent a challenge that clinicians and microbiologists have to address as a team in the absence of consensus or standardized protocols. (cun.es)
Add-back of low levels of NO, in the picomolar and nanomolar range, was able to induce biofilm dispersal and greatly increase the efficacy of various antimicrobial compounds. (omicsonline.org)
Biofilm cultures were treated with NEBB containing enzymes targeted at lipids, proteins, and sugars, also containing the mucolytic compound N-acetyl cysteine, along with antimicrobial extracts from cranberry, berberine, rosemary, and peppermint. (jmb.or.kr)
Using confocal laser scanning microscopy, we demonstrated that the drug damaged bacteria within an established biofilm showing that penetration did not limit the activity of this antimicrobial agent against a biofilm. (montana.edu)
The global spread of antibiotic resistance, coupled with increased tolerance to antimicrobial treatments in biofilm-associated bacteria, highlights the need for novel strategies to overcome treatment hurdles. (cdc.gov)

Biofilms are multicellular communities that were so far thought to be held together solely by a self-produced organic extracellular matrix. (nature.com)
Bacterial biofilms are matrix-bound multicellular communities. (biorxiv.org)

Phylogeny of the ring-forming bacterium Arcicella aquatica gen. nov., sp. (wikipedia.org)
For this reason this project focused in the marine biofilm-forming bacterium Pseudoalteromonas sp. (ifremer.fr)
The adsorption behavior and interaction mechanisms of extracellular polymeric substances (EPS) of a biofilm-forming bacterium, Pseudomonas aeruginosa OMCS-1, towards Cr, Pb, and Cd were investigated. (nitrkl.ac.in)
Background: The treatment of a bacterial infection when the bacterium is growing in a biofilm is a vexed issue. (iisc.ac.in)

To further investigate the pathogenesis of NTHi in respiratory infections, we developed a novel in vitro coculture model of NTHi biofilm formation on polarized human airway epithelial cells grown at the air-liquid interface. (nih.gov)
Biofilms are the predominant form of microbial life in the natural environment and although they are the leading cause of chronic infections, they are equally deeply connected to our ability to bioremediate waste and toxic materials. (dundee.ac.uk)
Furthermore, it showed that alternative therapeutic strategies have the potential to control biofilm related contamination and infections in animals and people. (lincoln.ac.uk)
Biofilm-related infections represent a serious health problem, accounting for 65- 80% of all infections. (cun.es)
The infections are generally chronic and characterized by the persistence of the microorganism, due to the increased resistance of biofilms to both the immune system and antimicrobials. (cun.es)
Targeting biofilms represents a novel approach against drug-resistant bacterial infections. (ubc.ca)
In developed countries, over 60% of bacterial infections requiring treatment involve biofilm formation [ 12 , 13 ]. (ophrp.org)
Anguka J. Effects of antimicrobials in vitro on Staphylococcus saprophyticus in planktonic and biofilm forms isolated from patients with urinary tract infections J. Environ. (pulsus.com)
The formation of a Pseudomonas aeruginosa biofilm, a complex structure enclosing bacterial cells in an extracellular polymeric matrix, is responsible for persistent infections in cystic fibrosis patients leading to a high rate of morbidity and mortality. (montana.edu)
This study confirms the potential of CSA-13 as a new strategy to combat persistent infections involving biofilms formed by P. aeruginosa. (montana.edu)
Combining Biofilm-Controlling Compounds and Antibiotics as a Promising New Way to Control Biofilm Infections. (helmholtz-hzi.de)
Therefore, a combination of biofilm-destroying compounds and antibiotics to handle biofilm infections is proposed. (helmholtz-hzi.de)
In this article, different approaches to combine biofilm-controlling compounds and antibiotics to fight biofilm infections are discussed, as well as the balance between biofilm formation and virulence. (helmholtz-hzi.de)
Going beyond the Control of Quorum-Sensing to Combat Biofilm Infections. (helmholtz-hzi.de)
In the medical field alone, biofilm infections have reportedly cost over five billion USD in additional healthcare expenses. (uwm.edu)
abstract = " Streptococcus pyogenes causes a multitude of local and systemic infections, the most common being pharyngitis in children. (lu.se)

Biofilms containing Pseudomonas aeruginosa, Micrococcus luteus, Stenotrophomonas maltophilia, Elizabethkingia anophelis, Cupriavidus metallidurans, and Methylobacterium fujisawaense were established in the CBR p-trap model for a period of 28 d. (cdc.gov)

Biofilm formation on Candida auris , C. albicans , and C. glabrata yeast strains. (cdc.gov)
On 3 separate occasions, 8 biofilms of each Candida species were grown in flat-bottomed, 96-well polystyrene microtiter plates and incubated for 24 h at 37°C, after which biomass was assessed by crystal violet assay ( 12 ). (cdc.gov)
Materials and Methods Candida albicans biofilms on acrylic resin plates were treated for 15 minutes with either nystatin, fluconazole, Polident, 220µM erythrosine + 1% (w/w) nano-TiO 2 + 15 J/cm 2 blue light photodynamic therapy (Ery PDT), or distilled water. (thieme-connect.de)
4 Han Y, Liu X, Cai Y. Effects of two peroxide enzymatic denture cleaners on Candida albicans biofilms and denture surface. (thieme-connect.de)
However, all Candida species produced a similar biofilm on acrylic resin, material used in dental prostheses. (biomedcentral.com)
We found that on silicone pads as well as in the Galleria model, biofilm formation and virulence depends on the Candida species. (biomedcentral.com)
Treatment of established biofilm with NEBB resulted in biofilm-disruption, involving significant reductions in biofilm mass and metabolic activity for Candida and both Staphylococcus species. (jmb.or.kr)

Clinical and genetic risk factors for biofilm-forming Staphylococcus a" by Megan Luther, M. Parente et al. (uri.edu)
The molecular and clinical factors associated with biofilm-forming methicillin-resistant Staphylococcus aureus (MRSA) are incompletely understood. (uri.edu)
Staphylococcus aureus is a major human pathogen that utilises many surface-associated and secreted proteins to form biofilms and cause disease. (sdu.dk)

This thesis focuses on the development and characterisation of a waveguide-based photonic sensing platform for the detection of biofilm. (dcu.ie)
This work proved the concept of employing a waveguide-based photonic platform for the early detection of biofilm formation, including the induction phase, and as such, I believe this system has immense potential for future applications as a label-free and real-time biosensor platform for bioenvironmental applications. (dcu.ie)

NTHi formed adherent biofilms on the apical surface airway epithelia with decreased susceptibility to antibiotics, and respiratory cells exhibited inflammatory and host defense responses-evidence of a dynamic host-pathogen interaction. (nih.gov)
Susceptibility to single antibiotics and to antibiotic combinations were not different between planktonically or biofilm-grown bacteria derived from sputum, as compared to those obtained by bronchoalveolar lavage and protected brush. (ersjournals.com)
In summary, the results in this thesis show that C. perfringens grown in biofilm mode independent of the source of isolation could reduce the effective susceptibility of antimicrobials. (lincoln.ac.uk)
Antifungal susceptibility of preformed biofilms was assessed, and virulence was studied using the Galleria mellonella model. (unicatt.it)

Retrieved bacteria were genotyped, and grown in planktonic cultures and as biofilms, and susceptibilities to individual antibiotics and to antibiotic combinations were determined. (ersjournals.com)
Combination of antibiotic and nanoparticles improved biofilm reduction compared to antibiotic alone, but this was not generally significant at the concentration tested. (lincoln.ac.uk)
Thus the peptide represents a novel strategy to potentiate antibiotic activity against biofilms. (ubc.ca)
Microbial biofilms are resilient, immune-evasive, often antibiotic-resistant health challenges, and increasingly the target for research into novel therapeutic strategies. (jmb.or.kr)
Overall, our findings provide fundamental new insight into crystalline biofilm formation by P. mirabilis, including the link between biofilm formation and antibiotic resistance in this organism, and indicate a potential role for efflux pump inhibitors in the treatment or prevention of P. mirabilis crystalline biofilms. (brighton.ac.uk)

Furthermore, relA and spoT mutations blocking production of (p)ppGpp replicated the effects of the peptide, leading to reduced biofilm formation. (ubc.ca)

The intrinsic resistance of microorganisms in biofilms towards standard antimicrobials makes it very difficult if not impossible to effectively apply current antifouling strategies without imposing a heavy stress on the marine environment. (omicsonline.org)

Although it is well established that pathogenic bacteria form biofilms extensively in nature to survive and resist adverse conditions by attaching to each other and to a substrate with extracellular polymeric substance (EPS). (lincoln.ac.uk)
In this study, we evaluated the ability of two types of biosurfactants (rhamnolipids and surfactin) to inhibit growth and biofilm formation ability of oral pathogenic bacteria such as Aggregatibacter actinomycetemcomitans , Streptococcus mutans , and Streptococcus sanguinis . (biomedcentral.com)
Controlling biofilms of gram-positive pathogenic bacteria. (helmholtz-hzi.de)

The observations were largely in concordance with the biofilm assay results. (biomedcentral.com)
The post-treatment biofilm mass was evaluated by crystal-violet staining, and metabolic activity was measured using the MTT assay. (jmb.or.kr)

This study aimed to test the effect of photodynamic therapy (PDT) on the inhibition and removal of biofilms containing multidrug-resistant Acinetobacter baumannii . (ophrp.org)
Therefore, removal of biofilms formed by periodontopathogenic bacteria prevents a variety of diseases. (biomedcentral.com)

Antibiofilm assays showed that examined extracts of spinach, chard, and orache caused changes in the biofilm-forming capacity of investigated bacterial pathogens. (jmbfs.org)
These results indicate that the biofilm habitat can reduce the efficacy of irradiation in eliminating pathogens from leafy vegetables. (usda.gov)
Oral pathogens that form persisters may cause recurrence of oral diseases via regrowth. (biomedcentral.com)
Therefore, inhibiting the growth of oral pathogens, as well as effectively preventing biofilm formation contribute to reducing these risks. (biomedcentral.com)
All these approaches lead to the destruction of the biofilm but not the killing of the pathogens. (helmholtz-hzi.de)
Biofilms formed by these health care-associated pathogens can lead to negative and costly health outcomes. (cdc.gov)
ABSTRACT This study evaluated the role of periodontal pathogens in 50 hospitalized patients with hospital- acquired pneumonia compared with 30 healthy controls. (who.int)
Dental plaque biofilm may promote oral and oropharyngeal colonization of respiratory pathogens in hospitalized subjects. (who.int)

In vitro studies have shown thatbiofilm-forming P. aeruginosa are significantly less susceptible to antibiotics compared to nonadherent forms 3 , 7 . (ersjournals.com)
PDT involving a combination of LED and Radachlorin significantly eliminated the biofilm of multidrug-resistant A. baumannii . (ophrp.org)
Both WT strains formed significantly richer biofilm on cFn than on plastic, indicating that cell anchoring with ECM strengthens the overall biofilm structure. (cdc.gov)
For P. aeruginosa , low doses of NEBB significantly reduced biofilm mass and metabolic activity while higher doses of NEBB increased biofilm mass and metabolic activity. (jmb.or.kr)
Results: Four compounds in this series significantly inhibit the growth and formation of biofilm by Mycobacterium smegmatis (mc(2) 155 strain) at 50 mu g/ml. (iisc.ac.in)
Biofilm bio-volume, mean thickness, and heterotrophic plate counts were significantly reduced and roughness coefficient significantly increased by Soda compared with D5W, Shake, or ATW. (cdc.gov)
Interestingly, internalization and persistence of planktonic bacteria varied significantly between strains, whereas biofilm bacteria were internalized in similar and higher numbers, and all strains persisted beyond 44 hours, showing a more homogenous phenotype. (lu.se)

NO, which is produced endogenously in mature biofilms, was shown to trigger a signaling pathway involving the intracellular secondary messenger cyclic di-GMP, which in turn activates a range of effectors that result in dispersal. (omicsonline.org)

Formation of biofilm in milking equipment could in turn cause bacterial contamination of bulk tank milk. (slu.se)

Bacteria with high biofilm-forming ability attach to host cells more easily or colonize medical devices and hospital environments, promoting horizontal gene transfer between bacteria in biofilms [ 14 , 15 ]. (ophrp.org)

The most active microorganisms in biofilms were Thermoanaerobacter sp. (edu.au)

Together they form a unique fingerprint. (dundee.ac.uk)

The present authors hypothesised that bronchoscopy with protected specimen brush may sample biofilm-forming bacteria adherent to the airway wall, whereas traditional sputum collection may not. (ersjournals.com)
Recent evidence suggests that P. aeruginosa may actually grow as dense communities of exopolysaccharide-encapsulated biofilms adherent to CF patients' airways 2 - 4 . (ersjournals.com)
A microbial biofilm is a community of adherent microbial cellular forms with properties that help protect the microbial community from disruption by physical, chemical, or immunological attack. (jmb.or.kr)

Following on the discovery of NO as a key signal regulating biofilm dispersal, he led a range of projects to develop innovative, non-toxic solutions to biofilm control in industrial and clinical settings. (omicsonline.org)
A study examining the biofilm-forming ability of multidrug-resistant A. baumannii strains recently isolated from a clinical sample indicated that all resistant bacteria exhibited higher biofilm-forming ability than the standard A. baumannii strain ATCC 19606 [ 11 ]. (ophrp.org)

ABSTRACT Optical density is a proxy of total biomass concentration and is commonly used for measuring the growth of bacterial cultures. (microbial-ecology.net)
Toxicity and mutagenicity, as well as changes in the bacterial biofilm biomass , physiological activity, and the number of living cells in its composition in the presence of glyphosate were assessed using the Escherichia coli model. (bvsalud.org)

In order to control biofilm formation, sequester proteolytic enzymes, and provide a biocompatible scaffold to support healing, the investigators utilize a purified collagen matrix containing polyhexamethylene biguanide (PCMP) in a case series of 9 wounds on 8 patients with multiple comorbidities who did not respond to previous conventional or adjuvant therapy. (medscape.com)

Microbial biofilms form on liquid/solid interfaces in nature, such as rocks and clay particles and decaying plant materials. (jmb.or.kr)
The results of this study suggest the potential of phages to control CPKP and other carbapenemase-producing organisms associated with microbial biofilms in the healthcare environment. (cdc.gov)

Average biofilm mass and metabolic activity for NEBB-treated biofilms were compared to the average of untreated control cultures. (jmb.or.kr)
For B. burgdorferi , we observed reduced biofilm mass, but the remaining residual biofilm showed a mild increase in metabolic activity, suggesting a shift from metabolically quiescent, treatment-resistant persister forms of B. burgdorferi to a more active form, potentially more recognizable by the host immune system. (jmb.or.kr)
All the studied concentrations of the pesticide completely or partially suppress the matrix and structure of the E. coli CDC F-50 biofilm formation, as well as the bacterial cells metabolic activity in the biofilm . (bvsalud.org)

We report here that myricetin, but not its glycosylated form, can remarkably decrease the production of several S. aureus virulence factors, including adhesion, biofilm formation, hemolysis and staphyloxanthin production, without interfering with growth. (nature.com)
Here, live respiratory epithelial cells were inoculated with broth-grown or biofilm bacteria of different M-types, as well as with isogenic mutants lacking common virulence factors. (lu.se)

Here, using mutation analyses and complementation, we demonstrated that both the ebp (encoding endocarditis and biofilm-associated pili) operon and srtC are important for biofilm production of E. faecalis strain OG1RF. (jci.org)
Eliminating (p)ppGpp expression after 2 days of biofilm growth by removal of arabinose from a strain expressing relA behind an arabinose-inducible promoter, reciprocated the effect of peptide added at the same time, leading to loss of biofilm. (ubc.ca)
Leaves of romaine lettuce and baby spinach were dip inoculated in a three strain cocktail of Salmonella and stored at 4 deg C for various times (0, 24, 48, 72h) to allow biofilms to form. (usda.gov)
Streptococcus sanguinis is another plaque-forming strain that has been reported to cause infective endocarditis by forming biofilms on the endocardium and heart valves [ 11 ]. (biomedcentral.com)
The primary objective of our study was to formulate a phage cocktail capable of targeting a CPKP strain (CAV1016) at different stages of colonization within polymicrobial drinking water biofilms using a CDC biofilm reactor (CBR) p-trap model. (cdc.gov)

The importance of biofilm in the context of increasing bacterial resistance to antibiotics]. (helmholtz-hzi.de)
The thesis elucidates the multi-metal resistance potential, biofilm-forming ability, and enhanced heavy metal removal efficiency of bacteria isolated from metal contaminated sites. (nitrkl.ac.in)

Abstract Introduction mass contamination by the new coronavirus caused enough damage for the pandemic to extend indefinitely Objective the objective of this study was to analyze the emotional factors acquired by students from a dentistry course during the COVID-19 pandemic period and the influences related to the resumption of face-to-face activities. (scielo.br)

Enzymes capable of cleaving essential components of the biofilm matrix, e.g. polysaccharides or extracellular DNA, and thus weakening the biofilm architecture have been identified. (helmholtz-hzi.de)

50% to 0.1% of Acquorsol solution completely inhibited the growth of four C. perfringens planktonic grown strains while 50% to 0.78% of Acquorsol solution completely prevented the growth of four C. perfringens biofilm grown strains. (lincoln.ac.uk)
When biofilms were grown during exposure to shear forces and to a continuous medium flow allowing the development of robust structures with a complex architecture, CSA-13 reached the bacteria entrapped in the biofilm within 30 min. (montana.edu)
DOI: https://dx.doi.org/10.1556/1886.2015.00049 Abstract Lyme borreliosis, caused by the spirochete Borrelia burgdorferi sensu lato, has grown into a major public health problem. (canlyme.com)
PTMs containing polymicrobial biofilms grown in autoclaved municipal tap water (ATW) supplemented with 5% dextrose in water (D5W), nutritional shake (Shake), sugar-based soft drink (Soda), or ATW were inoculated with K. pneumoniae ST258 KPC+ (ST258) or K. pneumoniae CAV1016 (CAV1016) and sampled after 7, 14, and 21 d. (cdc.gov)

Using the viable count method to determine the effect of treatment showed minimal (2%/0.1 log) to good (28% /2.5 log) biofilm inhibition and reduction. (lincoln.ac.uk)
Additionally, we performed hyphae inhibition and biofilm tests. (who.int)
E demonstrated no effective action against biofilms, though SE showed inhibition against biofilms of all strains. (who.int)

The draft genome identifying various proteins involved in biofilm formation ( 8 ), coupled with recent descriptions of aggregative and nonaggregative phenotypes, the latter of which are more virulent in vivo ( 9 ), indicate the possibility of heterogeneous C. auris biofilm formation, as described for C. albicans ( 10 ). (cdc.gov)
This study investigated ability of NTHi to form biofilms and its impact on airway epithelia using in vivo and in vitro analyses. (nih.gov)

However, studies have not previously been done to determine whether bacteria retrieved from sputum are more susceptible to antibiotics compared to bacteria retrieved directly from the airway biofilm. (ersjournals.com)
Further, the peptide was found to be synergistic with different classes of antibiotics to prevent and eradicate bacterial biofilms. (ubc.ca)
Additional studies on the effective elimination of biofilms containing multidrug-resistant bacteria are necessary, and we hope that a treatment method superior to sterilization with antibiotics will be developed in the future. (ophrp.org)
In this structure, they are well protected and often high dosages of antibiotics cannot clear infectious biofilms. (helmholtz-hzi.de)
[ 8-10 ] Biofilms differ genotypically and phenotypically from planktonic bacteria, resulting in higher tolerance to antibiotics and to the host immune response. (medscape.com)

We report that this highly virulent yeast has the capacity to form antifungal resistant biofilms sensitive to the disinfectant chlorhexidine in vitro. (cdc.gov)
Bacteria in mature microbial communities such as biofilms are relatively resistant to chemical treatments, but little is known about the response of leaf surface biofilms to irradiation. (usda.gov)
Bacteriophages have reemerged as potential biocontrol agents, particularly against biofilm-associated, drug-resistant microorganisms. (cdc.gov)

Biofilms are three-dimensional structures consisting of various microbial populations attached to a surface by extracellular polymeric substances (EPS) produced by these microorganisms [ 1 ]. (biomedcentral.com)
Bacteria secrete extracellular polymeric substances (EPS) that form biofilm-like structures in which the cells aggregate. (phys.org)
Secretion of extracellular polymeric substances (EPSs) by growing bacteria is an integral part of forming biofilm-like structures. (phys.org)

Biofilms can be located to almost every human body tissue and on exogenous devices such as catheters, pacemakers, prosthetic material, implants, urinary catheters, etc. (cun.es)
Proteus mirabilis forms extensive crystalline biofilms on urethral catheters that occlude urine flow and frequently complicate the management of long-term-catheterized patients. (brighton.ac.uk)

C. albicans displayed the greatest biofilm mass ( Figure 1 , panel A), consistent with previous findings ( 10 ). (cdc.gov)
At 6 hours, Ery PDT reduced the number of viable C. albicans in biofilms by 0.28log 10 CFU/mL, which was equal to the effect of fluconazole and Polident. (thieme-connect.de)
Conclusion Photodynamic therapy with erythrosine + nano-TiO 2 + blue light at low light power density (15 J/cm 2 ) was as effective at inhibiting C. albicans biofilm on acrylic resin as fluconazole and Polident. (thieme-connect.de)
On silicone pads, C. albicans and C. dubliniensis produced more biofilm (1.12 to 6.61 mg) than the other species (0.25 to 3.66 mg). (biomedcentral.com)

Low levels of the peptide led to biofilm dispersal, while higher doses triggered biofilm cell death. (ubc.ca)
Recently, the simple gas and ubiquitous biological signal nitric oxide (NO) was identified as a key regulator of biofilm dispersal. (omicsonline.org)

Effect of a berry polyphenolic fraction on biofilm formation, adherence properties and gene expression of Streptococcus mutans and its biocompatibility with oral epithelial cells. (cranberryinstitute.org)
Role of serotype and virulence determinants of Streptococcus pyogenes biofilm bacteria in internalization and persistence in epithelial cells in vitro. (lu.se)
Dive into the research topics of 'Role of serotype and virulence determinants of Streptococcus pyogenes biofilm bacteria in internalization and persistence in epithelial cells in vitro. (lu.se)

Quorum sensing and bacterial biofilms. (helmholtz-hzi.de)

In order to form an open promoter complex and begin transcription, σ54-RNA polymerase utilizes enhancer binding proteins as activators. (syr.edu)
The M3 protein, but not the M1 or M5 proteins, was required for optimal uptake and persistence of both planktonic and biofilm bacteria inside cells. (lu.se)

One problem with relying on sputum samples to guide therapy is that biofilm bacteria may not be adequately sampled via sputum, since sputum expectoration only samples P. aeruginosa that have detached and dispersed from the airway biofilm 5 , 6 . (ersjournals.com)
Thus, it is possible that PSB may directly recover P. aeruginosa bacteria that have aggregated as biofilms and adhered to the surface of the airway. (ersjournals.com)
The purpose of the present study was to determine whether the PSB can sample directly from the airway biofilm and, thus, more accurately represent the state of P. aeruginosa in CF patients' lungs. (ersjournals.com)

This pore-forming toxin has been reported as an important protein that mediates tissue damage promoted by S. aureus . (nature.com)

This study aimed to determine the effect of LW on biofilm formation and CPKP colonization in a P-Trap model (PTM). (cdc.gov)

The group A streptococcal collagen-like protein-1 (SCL1) is a key surface adhesin for biofilm formation. (cdc.gov)

We studied, for the first time, the effects of mutants defective in biomineralization and calcite formation on biofilm development, resilience and morphology. (nature.com)
Biofilm formed on nECM was comparable to that formed on commercial cFn and there was a statistically significant decrease in biofilm formed by both scl1 mutants as compared with the WT strains. (cdc.gov)
However, the biofilm-deficient phenotype of these mutants was not due to deficiencies in attachment to catheter biomaterials, and defects in later stages of biofilm development were indicated. (brighton.ac.uk)

We demonstrated that an intrinsic rise in carbon dioxide levels within the biofilm is a strong trigger for the initiation of calcite-dependent patterning. (nature.com)
The results clearly demonstrated that rhamnolipids inhibit biofilm formation after the initiation process, however, they do not affect attachment or maturation. (biomedcentral.com)

These compounds are unique in the sense that they are more potent against Mycobacterium smegmatis in the biofilm state compared to the planktonic form. (iisc.ac.in)

Biofilms represent a major form of microbial life on Earth and serve as a model active nematic system, in which activity results from growth of the rod-shaped bacterial cells. (biorxiv.org)
Biofilm formation relies on three main parameters including the bacterial cells, the surface attached, and the surrounded medium but the attachment of bacteria to the surface is the initial process of biofilm. (uthm.edu.my)

0.05) in biofilm mode and under different heavy metal concentrations. (nitrkl.ac.in)

Anatomy1

Organisms9

Diseases3

Chemicals and Drugs13

Analytical, Diagnostic and Therapeutic Techniques and Equipment7

Phenomena and Processes16

Technology, Industry, Agriculture2

Information Science2

Scanning electron m2

Known as biofilms3

Surfaces14

Isolates11

Strong biofilm4

Anti-biofilm6

Antimicrobial7

Multicellular communities2

Bacterium4

Infections16

Pseudomonas1

Candida7

Staphylococcus3

Detection of biofilm2

Susceptibility4

Antibiotic5

Reduced biofilm formation1

Antimicrobials1

Pathogenic bacteria3

Assay2

Removal of biofilms2

Pathogens8

Significantly7

Mature biofilms1

Formation of biofilm1

Bacteria in biofilms1

Microorganisms in biofilms1

Unique fingerprint1

Adherent3

Clinical2

Biomass2

Control biofilm1

Microbial biofilms2

Metabolic activity3

Virulence factors2

Strain5

Resistance2

Contamination1

Enzymes1

Grown4

Inhibition3

Vivo2

Antibiotics5

Resistant3

Extracellular polymeric3

Catheters2

Albicans4

Dispersal2

Epithelial cells3

Quorum1

Proteins2

Aeruginosa3

Aureus1

Colonization1

Adhesin1

Mutants3

Initiation2

Planktonic form1

Bacterial cells2

0.051