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Stationary phaseMass spectrometrySpectraFluorescenceLayer chromatographyExchange chromatographyChromatographicAdsorbentPolymersSoluteSeparationCompoundsMoleculeMolarMethanolPurificationMetabolismRetentionBiocompatibilityChemistryAdvantagesCompoundSpectroscopyDistinctLiquidConcentrationEluentMethodSolventProcessDiffusionMaximumMeansThinBandsTimeWaterComponents
Stationary phase17
  • The main advantage of column chromatography is the relatively low cost and disposability of the stationary phase used in the process. (wikipedia.org)
  • For the dry method, the column is first filled with dry stationary phase powder, followed by the addition of mobile phase, which is flushed through the column until it is completely wet, and from this point is never allowed to run dry. (wikipedia.org)
  • For the wet method, a slurry is prepared of the eluent with the stationary phase powder and then carefully poured into the column. (wikipedia.org)
  • The individual components are retained by the stationary phase differently and separate from each other while they are running at different speeds through the column with the eluent. (wikipedia.org)
  • The stationary phase or adsorbent in column chromatography is a solid. (wikipedia.org)
  • The most common stationary phase for column chromatography is silica gel, the next most common being alumina. (wikipedia.org)
  • There is an important ratio between the stationary phase weight and the dry weight of the analyte mixture that can be applied onto the column. (wikipedia.org)
  • The eluent is optimized in small scale pretests, often using thin layer chromatography (TLC) with the same stationary phase, using solvents of different polarity until a suitable solvent system is found. (wikipedia.org)
  • Bare silica gel is an acceptable stationary phase. (tsijournals.com)
  • The chemical attachment of suitable groups, usually C18 alkyl groups, onto a carrier, primarily silica gel, produces the practically applicable volume of LC stationary phase. (tsijournals.com)
  • A form of gas chromatography in which the moving phase is a gas and the stationary phase is a surface-active sorbent (charcoal, silica gel, or activated alumina). (termwiki.com)
  • The classical preparative chromatography column, is a glass tube with a diameter from 5 mm to 50 mm and a height of 5 cm to 1 m with a tap and some kind of a filter (a glass frit or glass wool plug - to prevent the loss of the stationary phase) at the bottom. (blogspot.com)
  • The eluent is optimized in small scale pretests, often using thin layer chromatography (TLC) with the same stationary phase. (blogspot.com)
  • Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. (lookformedical.com)
  • Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix. (lookformedical.com)
  • A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. (lookformedical.com)
  • It is commonly used as a desiccating agent and as a stationary phase for CHROMATOGRAPHY. (lookformedical.com)
Mass spectrometry2
Spectra4
  • The composition of the eluent flow can be monitored and each fraction is analyzed for dissolved compounds, e.g. by analytical chromatography, UV absorption spectra, or fluorescence. (wikipedia.org)
  • In principle, peak positions of the absorption and fluorescence spectra are in a linear relationship with the permittivity of the medium molecules, as theoretically derived by Lippert and Mataga 9 . (nature.com)
  • The chemical interaction of the dimethylgermole-containing π-conjugated polymer with fluoride was also examined in terms of the changes observed in the UV-vis absorption spectra. (nature.com)
  • UV absorption spectra arises in which molecule or atoms outer electrons absorb energy, undergoes transition from lower energy level to higher energy level. (pharmaclub.in)
Fluorescence3
  • The novel nanosystem (ZnPR-IONP) has been thoroughly characterized by a variety of techniques such as UV-Vis absorption spectroscopy, fluorescence spectroscopy, X-ray photoloectron spectroscopy (XPS) and transmission electron microscopy (TEM). (docksci.com)
  • 1 In particular, much attention has been paid to near-infrared (NIR) fluorescence imaging within the wavelength range of 700-900 nm due to its low absorption and autofluorescence from organisms and tissues in the NIR spectral range, rendering minimal background interference, deep tissue penetration, and high sensitivity for cancer imaging. (dovepress.com)
  • 2 , 3 The heart of NIR imaging lies in the development of fulfilling fluorochromes, which must have a high molar absorption coefficient for intense fluorescence, good biocompatibility with low toxicity, and sufficient stability and water solubility in the complex biological environment. (dovepress.com)
Layer chromatography2
Exchange chromatography3
Chromatographic1
  • At 35 °C, two separate liquid chromatographic critical ranges were discovered using non-end-capped silica gel C-18 column packing, poly (methyl methacrylate)s (PMMAs), and mixed eluents including acetonitrile (ACN) and toluene. (tsijournals.com)
Adsorbent2
  • A column is prepared by packing a solid adsorbent into a cylindrical glass or plastic tube. (wikipedia.org)
  • Results: It was determined that the optimum extraction process for the addition was 10 times 85% ethanol for 90 minutes' extraction, the best purification process for the expansion agent cyclohexane-chloroform- acetone was 4:3:3, column height and diameter was 7:1, adsorbent and sample volume was 5.5:1. (bvsalud.org)
Polymers2
Solute2
  • Absorption (enthapic partition), the distribution of a solute between the volumes of the mobile and stationary phases, is another LC retention mechanism. (tsijournals.com)
  • Chromatography on non-ionic gels without regard to the mechanism of solute discrimination. (lookformedical.com)
Separation3
  • A faster flow rate of the eluent minimizes the time required to run a column and thereby minimizes diffusion, resulting in a better separation. (wikipedia.org)
  • A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million. (lookformedical.com)
  • Through the verification of three batches of amplification process, it was shown that the model fit well, and the separation and purification of toad poison ligand by silicone column chromatography had the advantages of simple operation, fast separation speed, and good separation effect. (bvsalud.org)
Compounds6
  • compounds move through the column at different rates, allowing them to be separated into fractions. (wikipedia.org)
  • A thin-layer chromatograph can show how a mixture of compounds will behave when purified by column chromatography. (wikipedia.org)
  • The mobile phase or eluent is a solvent or a mixture of solvents used to move the compounds through the column. (wikipedia.org)
  • Contrary to common misconception, methanol alone can be used as an eluent for highly polar compounds, and does not dissolve silica gel. (wikipedia.org)
  • The similarities and differences of two-dimensional liquid chromatography of low-molecular-mass and polymeric compounds are discussed. (tsijournals.com)
  • Column chromatography in chemistry is a method used to purify individual chemical compounds from mixtures of compounds. (blogspot.com)
Molecule2
  • The oxazine compound has a structure of a general formula F. The oxazine compound described in the disclosure is a photo/acoustic dynamic active organic molecule with near-infrared absorption-emission function as well as photosensitivity and acoustic sensitivity. (justia.com)
  • Ans) When a molecule absorbs the Infrared radiation, it vibrates and gives rise to packed Infrared(IR) absorption spectrum. (pharmaclub.in)
Molar1
Methanol2
  • The residue was purified by column chromatography on silica gel (methanol-dichloro-methanegradientelution)toaffordGlcNGc(0.8g)in15%yield overall. (glassbox.tv)
  • When the extract was separated on a Cosmosil column, the eluate with 70% methanol showed the most potent ability to increase [ 3 H]vinblastine uptake. (aspetjournals.org)
Purification1
  • In the purification technology, most of the cation exchange resin method, reverse chromatography purification technology is also a little research. (rainbowextract.com)
Metabolism1
  • Enrichment analysis showed that ASO intake mainly affected sphingolipid metabolism, fat digestion and absorption, glycerolipid metabolism and glycerophospholipid metabolic pathways in serum and the brain. (bvsalud.org)
Retention4
  • It is chosen so that the retention factor value of the compound of interest is roughly around 0.2 - 0.3 in order to minimize the time and the amount of eluent to run the chromatography. (wikipedia.org)
  • Steric exclusion, based on changes in conformational entropy of coiled macromolecules entering the pores of the column packing, is the basic retention mechanism. (tsijournals.com)
  • Basic information on retention mechanisms in synthetic polymer liquid chromatography is provided. (tsijournals.com)
  • Q15) How to calculate Retention factor in paper chromatography? (pharmaclub.in)
Biocompatibility1
  • In addition, due to more advantages of silica, such as nontoxicity, biocompatibility chemical inertness, no swelling, and transparency, it is a possible option to develop the nontoxic, biocompatible, and recoverable Zn 2+ -selective fluorescent sensors on Fe 3 O 4 @SiO 2 NPs surface. (hindawi.com)
Chemistry1
Advantages1
  • Through the amplification process validation of three batches, it showed that the WZG prepared by the optimized excipients and process parameters have the advantages of high molding rate, good melting and low moisture absorption rate. (bvsalud.org)
Compound3
  • After drying (NaiSO 4 ) and evaporation in vacuo, the resulting brownish solid was purified by column chromatography (SiO 2 ) using pentane/ethyl acetate (9:1) to give 1.04 g (60 percent) of the title compound, mp 137-138 0 C (lit. (ambeed.com)
  • The residue was purified by column chromatography (hexanes/ethyl acetate: 9/1) to afford compound 2002 as a white solid (270 mg, 27percent yield). (ambeed.com)
  • Ans) Qualitative analysis involves identification of the compound or chemical based on their chemical(absorption, emission )or physical properties(e.g Melting point, boiling point). (pharmaclub.in)
Spectroscopy1
  • Gas chromatography-mass spectroscopy (GCMS), on the other hand, is another advanced and sophisticated technology used to identify and quantify unknown phytocompound in a complex mixture. (springeropen.com)
Distinct1
Liquid2
Concentration1
  • Mulberry leaves were extracted with 70% ethanol, concentrated, macroporous adsorption resin, over-cation exchange resin, distilled water, 0.5mol/L ammonia elution, purified by silica gel after concentration, combined with pure components containing DNJ, concentrated and vacuum dried. (rainbowextract.com)
Eluent4
  • Eluent is slowly passed through the column to advance the organic material. (wikipedia.org)
  • During the entire chromatography process the eluent is collected in a series of fractions. (wikipedia.org)
  • Adsorption is usually caused by polar interactions between active sites on macromolecules and on the column packing surface, which are influenced by the polarity of the eluent and, less frequently, by temperature. (tsijournals.com)
  • Often a spherical eluent reservoir or an eluent-filled and stoppered separating funnel is put on top of the column. (blogspot.com)
Method1
  • Two methods are generally used to prepare a column: the dry method and the wet method. (wikipedia.org)
Solvent2
  • Column chromatography can be done using gravity to move the solvent, or using compressed gas to push the solvent through the column. (wikipedia.org)
  • A solvent reservoir may be attached at the top of the column. (wikipedia.org)
Process1
  • Results: The optimum proportion of excipients was the dextrin with 1:3 of the dosage of dry paste powder, 1:15 carboxymethy starch sodium, 1:90 of the micro-powder silica gel, the best granulation process for rolling wheel pressure 9.5 MPa, rolling speed 14.0 Hz, and feed speed 13.2 Hz. (bvsalud.org)
Diffusion1
  • Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. (lookformedical.com)
Maximum1
  • The absorption maximum and onset of the obtained polymer were observed at 465 and 535 nm, respectively, in the UV-vis spectrum, from which the optical band gap of the polymer was estimated to be 2.31 eV. (nature.com)
Means1
Thin1
Bands1
  • Third, when several fluorescent dyes are used concurrently, simultaneous excitation becomes difficult because the absorption bands of the dyes are widely separated. (justia.com)
Time2
Water1
  • Infrared absorption measurements of the broad band for water near 3500 cm -1 can also sometimes be used fro detecting water in non-hydroxylic substances. (glassbox.tv)
Components1
  • For silica column chromatography, this ratio lies within 20:1 to 100:1, depending on how close to each other the analyte components are being eluted. (wikipedia.org)