• However, several recently developed technologies now allow one to systematically monitor the change of protein glycosylation and glycans in various biological fluids from a large number of individuals. (nih.gov)
  • Oncotarget published " Trends in oligomannosylation and α1,2-mannosidase expression in human cancers " which reported that aberrant protein glycosylation is a prominent cancer feature. (oncotarget.com)
  • The aberrant glycans and glycoconjugates were reported to be the markers for diagnosis and prognostic prediction of the cancer. (tci-thaijo.org)
  • Traditionally, it has been very difficult to study and monitor the alteration of glycosylation and glycans in relation to aging and early initiation of AD. (nih.gov)
  • Up-regulation of sialic acid-containing glycans, termed hypersialylation, is a common feature of cancer-associated glycosylation, driving disease progression and immune escape through the engagement of Siglec receptors on tumor-infiltrating immune cells. (bvsalud.org)
  • The deficiency of hinge-region glycosylation of serum IgA1 showed an increased tendency to self-aggregation and/or the increased binding to circulating glycoproteins and enhanced reaction with specific IgG antibodies directed against IgA1 hinge O-glycans. (biomedcentral.com)
  • Finally, we apply our method on mucin-type O-glycans, gangliosides, and site-specific compositional glycosylation data to reveal tissues and disease-specific glycan presentations. (omicsdi.org)
  • It has been a popular belief that diseases caused by abnormal glycosylation of proteins and lipids contribute to the pathology of those diseases. (wikipedia.org)
  • Glycosylation is a post-translational modification in which a sugar (or carbohydrate) is attached to a hydroxyl or other functional group of a macro molecule (such as DNA, lipids and proteins). (nih.gov)
  • Glycosylation, a post-translational modification affects the biophysical, functional and biochemical properties and provides structural complexity for these proteins. (amrita.edu)
  • Parkinson's Disease (PD) associated state of neuroinflammation due to the aggregation of aberrant proteins is widely reported. (biorxiv.org)
  • Lectin arrays are widely used tools for analyzing glycosylation in proteins and cells. (muni.cz)
  • Glycosylation of those proteins was proven to influence their subcellular locations. (frontiersin.org)
  • Glycosylation is an important post-translational modification of proteins, and glycoproteins are widely involved in various important cellular biological processes and diseases ( 1 ). (frontiersin.org)
  • The epitope that EBC-129 binds to is a specific glycosylation 1 site that is conserved on both carcinoembryonic antigen-related cell adhesion molecules (CEACAM) 5 and 6, which are proteins over-expressed in cancer cells. (a-star.edu.sg)
  • Additionally, evaluation of the EV cargo revealed proteins associated with an AG prostate cancer phenotype elevated in FUT8-transformed EVs, while capturing aberrant activity of the cells endocytic-related machinery. (cancer.gov)
  • UDP-GlcNAc is then used for N-linked and O-linked glycosylation in the ER and Golgi and for O-GlcNAc modification of nuclear and cytoplasmic proteins by OGT (O-GlcNAc transferase). (biomedcentral.com)
  • These glycoproteins are widely involved in important biological processes, such as antigen presentation, cytokine production and glycosylation progression. (frontiersin.org)
  • Dive into the research topics of 'Glycosylation Profiling of Glycoproteins Secreted from Cultured Cells Using Glycan Node Analysis and GC-MS'. Together they form a unique fingerprint. (elsevierpure.com)
  • Glycosylation and complex carbohydrates have been reported to play many critical roles in the early pathogenesis and progression of AD, but the potential of these molecules to serve as biomarkers and targets of disease intervention remains largely unexplored. (nih.gov)
  • Researchers have speculated that the RNA-glycan linkage is likely not due to the direct glycosylation of RNA bases. (wikipedia.org)
  • Despite the importance of glycosylation and altered glycan structures in AD, the aberrant molecular and biochemical function of these glycosylated molecules to serve as disease modifiers remain largely elusive. (nih.gov)
  • Large-scale analysis of glycosylation in organisms includes glycomics (glycan profiling) and glycoproteomics (glycosylation site profiling) ( 5 ). (frontiersin.org)
  • Interestingly, preliminary data from the Evans and Engle labs suggest that elevated levels of carbohydrate antigen (CA) 19-9 glycosylation directly cause pancreatitis and stimulate remodeling of the pancreatic microenvironment. (themarkfoundation.org)
  • Therefore, the goal of this Notice of Special Interest (NOSI) is to invite research projects using state-of-the-art methods of protein carbohydrate analyses to understand the potential impact of glycosylation on the etiology of AD and biomarker discovery. (nih.gov)
  • Aberrant sialylated carbohydrate synthesis is frequently noted in various cancers. (biomedcentral.com)
  • Glycosylation alterations are a hallmark of pancreatic cancer. (rupress.org)
  • This review discusses the contributions of aberrant glycosylation to protumorigenic signaling, metastasis, and remodeling the tumor immune microenvironment in pancreatic cancer. (rupress.org)
  • Glycosylation is known to affect various cellular and physiological functions including regulation of enzymatic activities, cell differentiation and morphogenesis. (nih.gov)
  • This study establishes evidence of how altered cellular glycosylation enzyme expression impacts EV vesicle abundance and the protein content of EVs secreted from prostate cancer cells via dysregulation of the cell's endocytic pathway. (cancer.gov)
  • Therefore, we analyzed mutation frequencies of genes of the cellular glycosylation machinery in MSI-H tumors, focusing on frameshift mutations in coding MNRs (cMNRs). (embl.de)
  • lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3). (omicsdi.org)
  • The mechanisms contributed to aberrant galactosylation of IgA1 molecule in patients with IgAN were still unclear. (biomedcentral.com)
  • Glucocorticoids Reduce Aberrant O-Glycosylation of IgA1 in IgA Nephropathy Patients. (imtm.cz)
  • Importantly, changes in glycosylation patterns were observed in various tumors, and the aberrant glycosylation is often associated with malignancy potential, tumor immune surveillance, and patients´ prognosis. (muni.cz)
  • However, aberrant glycosylation has been largely ignored in microsatellite unstable (MSI-H) colorectal tumors. (embl.de)
  • It also shows how differences in EV protein cargo, including glycosite occupancy and glycosylation patterns, may contribute to prostate carcinogenesis - making a case for EV-based diagnostics that incorporate glycosylation information for prostate cancer screening. (cancer.gov)
  • This process results in differing glycosylation patterns that can be used to detect cancer. (sc.edu)
  • In order to study these unique glycosylation patterns, synthetic lectins have been developed that are used in a cross-reactive sensor array. (sc.edu)
  • The discovery of glycoRNAs points researchers in the direction of an alternative molecule that may be responsible for the disrupted glycosylation network in human diseases. (wikipedia.org)
  • The fabricated lectin arrays will be used to map the glycosylation changes at different stages of cell differentiation and under different physiopathological conditions. (muni.cz)
  • Mass spectrometry and lectin arrays are common technologies publicly adopted in glycosylation analysis. (frontiersin.org)
  • The glycosylation of cells reflects the biological species, tissue, and physiological state of the organism. (muni.cz)
  • Aberrant expression and glycosylation of mucins contribute to tumour survival and proliferation in many cancers, which in turn activates numerous signalling pathways such as NF-kB, ERα, HIF, MAPK, p53, c-Src, Wnt and JAK-STAT, etc. (amrita.edu)
  • After restricting the N-glycosylation profile of thymocytes to high-mannose structures, using specific glycoengineered mice (Rag1CreMgat1fl/fl), we showed remarkable defects in key developmental checkpoints, including ß-selection, regulatory T-cell generation and γδT-cell development, associated with increased susceptibility to colon and kidney inflammation and infection. (for2953-sia.de)
  • However, other two somatic mutations of C1GALT1C1 gene contributed to the aberrant galactosylation of Tn syndrome [ 14 ]. (biomedcentral.com)
  • From the earliest onset of cancer, changes can be detected in the type and amount of carbohydrates expressed by the cancer cells, i.e., aberrant glycosylation. (sc.edu)
  • Aberrant glycosylation can also greatly affect the potential of cancer cells to metastasize and can be used to stage the disease as well. (sc.edu)
  • While many tumour-associated glycoepitopes have been reported, advances in glycoanalytics continue to uncover new associations between glycosylation and cancer. (oncotarget.com)
  • We previously reported aberrant glycosylation of lumican, one of small leucine-rich proteoglycans (SLRPs), in aortic valves in patients with aortic valve stenosis (AS). (oatext.com)
  • Comparative analysis of transferrin and IgG N-glycosylation in two human populations. (mpg.de)
  • Glycosylation is a major posttranslational modification present in virtually all cells, including T-lymphocytes, that regulates activity/functions. (for2953-sia.de)
  • Then amounts of three molecules with or without removal of N-glycosylation by PGNase were compared between the AS-C and AS-N areas. (oatext.com)
  • As a result, the amounts of PRELP with moderate and/or low amounts of N-glycosylation were found decreased in the AS-C areas compared to the AS-N areas. (oatext.com)
  • Mass spectrum-based high-throughput characterization of glycosylation as well as proteomic analysis has been used for years ( 7 - 11 ). (frontiersin.org)