Oocytes have been activated using calcium ionophore (A23187) in previous clinical cases of ICSI fertilization failure. (nih.gov)
However, studies on the efficiency of calcium ionophore (A23187) activation, its effects on the developmental potential of embryos, and its effects on pregnancy outcomes after embryo transfer are relatively limited. (nih.gov)
In this study, we investigated the safety and long-term efficacy of calcium ionophore (A23187) by analyzing its effects on fertilization, embryonic development, aneuploidy, and pregnancy outcomes in patients undergoing preimplantation genetic testing (PGT) cycles. (nih.gov)
Ionomycin is a calcium ionophore commonly derived from produced by the bacterium Streptomyces conglobatus, which means that it helps to transport calciumions across cell membranes. (sigmaaldrich.com)
12. Inhibition of HSP70 expression by calcium ionophore A23187 in human cells. (nih.gov)
Histamine release stimulated by calcium ionophore A23187 was also inhibited by this compound. (eurekamag.com)
This response was increased by cotreatment with the calcium ionophore A23187. (nih.gov)
Stimulating secretion with elevated extracellular potassium, with the calcium ionophore ionomycin, or with thyrotropin releasing hormone or vasoactive intestinal polypeptide resulted in abundant exocytic structures. (rupress.org)
Activation of the gene by KCl-induced depolarization or the calcium ionophore A23187 was dependent upon and synergistic with cAMP in PC12 and C6-glioma cells, but neither depolarization nor treatment with A23187 affected cAMP levels. (jneurosci.org)
Low density lipoprotein modified by oxidation (ox-LDL) induced a concentration-dependent inhibition of EDRF activity that had been released from bovine aortic endothelial cells (BAEC) stimulated with bradykinin or the calcium ionophore A23187. (jci.org)
The calcium ionophore A-23187 increases [Ca2+]c in the endothelial cells. (fapesp.br)
Vascular efects induced by calcium ionophore A23187 in renal hypertensive rat aorta. (fapesp.br)
During the past year we studied the regulation of NPY mRNA abundance in PC12 cells by cAMP, phorbol esters, glucocorticoids, and calcium ionophore. (nih.gov)
Acrosome reaction can therefore be induced through co-incubation of spermatozoa with calcium ionophore A23187 in vitro [31], [32]. (biosemiotics2013.org)
The calcium ionophore A23187 has been reported not to induce EPO production by human hepatoma (Hep3B) cells (Goldberg 1988). (lavoixdesrroms.org)
Compounds used for the study included modulators of the extracellular Ca2+ concentration (CaCl2, EGTA), a calcium ionophore (ionomycin), membrane permeant Ca2+ chelators (BAPTA-AM, EGTA-AM) and an endoplasmic reticulum Ca2+-ATPase inhibitor (thapsigargin). (lavoixdesrroms.org)
Amylase secretion from isolated acini stimulated by CCK-8, carbachol, phorbol ester, forskolin, and calcium ionophore (A23187) was present at birth. (uaeu.ac.ae)
Adenosine inhibits superoxide generation by PMNs in response to stimuli which cause a rise in the intracellular free calcium concentration, such as formylmethionyl-leucyl-phenylalanine (FMLP) or the calcium ionophore A23187. (docksci.com)
Using a calcium ionophore A23187 and a specific inhibitor of calpains (CS peptide) we have also demonstrated for the first time that activated m-calpain is able to decrease both PKCa and MARCKS levels in caveolae. (myobase.org)
Bradykinin2
Isofluane attenuated pulmonary vasorelaxation induced by bradykinin, A23187, and cromakalim but had no effect on relaxation induced by acetylcholine or SIN-1. (silverchair.com)
Neither the nitric oxide-mediated nor the prostacyclin-mediated components of relaxation induced by bradykinin and A23187 were altered by ISO. (silverchair.com)
Vitro2
We propose the development and study of novel sperm treatments for cattle in vitro production: transient application of Ca2+ ionophore to sperm (A23187 pulse), and the use of Sperm Energy restriction and Recovery (SER). (umass.edu)
Therefore, we studied the inhibitory effect of increasing concentrations of verapamil on both in vitro antigen-induced degranulation and ionophore A23187-induced release of labelled serotonin by rat peritoneal mast cells. (epiphanyasd.com)
Cations2
Ionophores selective for cations and anions have found many applications in analysis. (wikipedia.org)
Among divalent cations, the ionophore monensin shows high activity and selectivity for the transport of lead ions (Pb 2+ ) across phospholipid membranes. (nih.gov)
RBCs1
According to Setty and colleagues, PS can be exposed on human RBC by the action of ionophore on control RBCs. (medscape.com)
Endothelial cells3
These data suggest that Ca2+ influx induced by A23187, in the endothelial cells, is involved in the activation of eNOS. (fapesp.br)
Therefore, this study aims to investigate the cellular mechanisms of the vascular relaxation caused by the [Ca2+]c increased influx promoted by the Ca2+ ionophore in the endothelial cells of normotensive (2K) and renal hypertensive (2K-1C) rat aorta and to investigate the contribution of ROS for these effects. (fapesp.br)
With this purpose, we will use the A23187 in the vascular reactivity studies in the isolated aortic rings as well the flow cytometry studies in isolated endothelial cells from the 2K and 2K-1C rat aorta in order to measure ROS. (fapesp.br)
Nigericin1
The term ionophore (from Greek ion carrier or ion bearer) was proposed by Berton Pressman in 1967 when he and his colleagues were investigating the antibiotic mechanisms of valinomycin and nigericin. (wikipedia.org)
Inhibitor2
A comparative analysis with A23187 and the endoplasmic reticulum Ca(2+)-ATPase inhibitor thapsigargin. (nih.gov)
Both palmitate- and oxalate- activated Ca 2+ accumulation in the presence of GTP was inhibited by the anion transport inhibitor 4,4-diisothiocyanatostiibene-2, 2disulfonic acid (DIDS) suggesting their entry is mediated by a similar nonselective anion channel To examine the nature of the palmitate- and oxalate-induced Ca 2+ complexes, the releasabililty of Ca 2+ in response to InsP, and the ionophore A23187 were compared. (psu.edu)
It appears that the palmitate-Ca 2+ complex lies close to the ER membrane and hence highly accessible to the action of A23187. (psu.edu)
Membranes3
Ionophores catalyze ion transport across hydrophobic membranes, such as liquid polymeric membranes (carrier-based ion selective electrodes) or lipid bilayers found in the living cells or synthetic vesicles (liposomes). (wikipedia.org)
Ionophores are chemical compounds that reversibly bind and transport ions through biological membranes in the absence of a protein pore. (wikipedia.org)
Ionophores modify the permeability of biological membranes toward certain ions to which they show affinity and selectivity. (wikipedia.org)
Hydrophilic1
Ions are bound to the hydrophilic center and form an ionophore-ion complex. (wikipedia.org)
The co-incubation of spermatozoa with anti-VDAC2 antibody did not impact the acrosomal integrity and acrosome reaction, but inhibited ionophore "type":"entrez-nucleotide","attrs":"text":"A23187″,"term_id":"833253″,"term_text":"A23187″A23187-induced intracellular Ca2+ increase. (biosemiotics2013.org)
Mast cells1
Broncho-Vaxom (BV) inhibited in dose-dependent manner the release of histamine from and degranulation of isolated rat peritoneal mast cells stimulated with compound 48/80 and the ionophore A23187. (nih.gov)
Effect2
The effect of MPTP opening at low Ca 2+ concentrations is similar to that of Ca 2+ -ionophore, A-23187. (ukrbiochemjournal.org)
The Ca2+ ionophore A23187 mimicked the effect of BzATP suggesting that an intracellular Ca2+ increase was sufficient to evoke MV shedding. (unife.it)
Pulmonary2
In rats exposed to fine TiO2, A23187 infusion elicited vasodilations that were blunted in proportion to pulmonary particle deposition. (cdc.gov)
In rats exposed to ultrafine TiO2, A23187 infusion produced arteriolar constrictions or significantly impaired vasodilator responses as compared to the responses observed in control rats or those exposed to a similar pulmonary load of fine particles. (cdc.gov)
Rats2
The actions of monensin on Pb dynamics in rats suggest the possibility of using ionophores in the treatment of human Pb intoxication. (nih.gov)
In control rats, A23187 infusion produced dose-dependent arteriolar dilations. (cdc.gov)
Several chemical factors affect the ionophore activity. (wikipedia.org)
The activity of an ionophore-metal complex depends on its geometric configuration and the coordinating sites and atoms which create coordination environment surrounding the metal center. (wikipedia.org)
Se les registró el peso durante 17 semanas y, en la semana 15 se les administró suero fisiológico (control negativo), dicromato de potasio 25 mg/kg de peso corporal (pc) (control positivo) y tartrazina a dosis de 0,75 mg/kg pc, 7,5 mg/kg pc y 75 mg/kg pc, durante siete días, a excepción del control positivo que fue en dosis única. (bvsalud.org)
Los tres tratamientos con tartrazina no causaron diferencias significativas en el peso de ratones a la semana 15, pero sí produjeron diferencias significativas en la frecuencia de linfocitos micronucleados, siendo el tratamiento con tartrazina de 75 mg/kg pc el de mayor efecto genotóxico, induciendo un promedio de 1,63 ± 0,08 linfocitos micronucleados, comparado con el control positivo que generó un promedio de 1,42 ± 0,08 linfocitos micronucleados. (bvsalud.org)